HMAP-tool 1.0__tar.gz

hmap_tool-1.0/HMAP/HMAP.py

@@ -0,0 +1,1298 @@
+import pyro
+import pyro.distributions as dist
+from pyro.optim import ExponentialLR
+from pyro.infer import SVI, JitTraceEnum_ELBO, TraceEnum_ELBO, config_enumerate
+
+import torch
+import torch.nn as nn
+from torch.utils.data import DataLoader
+from torch.distributions.utils import logits_to_probs, probs_to_logits, clamp_probs
+from torch.distributions import constraints
+from torch.distributions.transforms import SoftmaxTransform
+
+import argparse
+import os
+import time as tm
+import random 
+import itertools
+import pandas as pd
+import numpy as np
+import datatable as dt
+import networkx as nx
+from pathlib import Path
+import warnings
+warnings.filterwarnings("ignore")
+
+from .utils.utils import convert_to_tensor, tensor_to_numpy, CustomDataset, CustomDataset2, CustomDataset4
+from .utils.custom_mlp import MLP, Exp
+from .graph.graph import compute_metacell_diffusion_kernel,visualize_metacell_igraph_with_fa2
+from .atac import binarize
+
+from tqdm import tqdm
+from typing import Literal
+import dill as pickle
+import gzip
+import scanpy as sc 
+from scipy import sparse
+
+def set_random_seed(seed):
+    # Set seed for PyTorch
+    torch.manual_seed(seed)
+    
+    # If using CUDA, set the seed for CUDA
+    if torch.cuda.is_available():
+        torch.cuda.manual_seed(seed)
+        torch.cuda.manual_seed_all(seed)  # For multi-GPU setups.
+    
+    # Set seed for NumPy
+    np.random.seed(seed)
+    
+    # Set seed for Python's random module
+    random.seed(seed)
+
+    # Set seed for Pyro
+    pyro.set_rng_seed(seed)
+    
+class HMAP(nn.Module):
+    def __init__(self,
+                 input_size: int,
+                 undesired_size: int = 0,
+                 codebook_size: int = 30,   # size of metacell codebook
+                 supervised_mode: bool = False,
+                 use_cell_factor: bool = False,
+                 d_dim: int = 3,            # dimension of a metacell variable
+                 d_dist: Literal['normal','laplacian','caucy','studentt','vonmises','gumbel'] = 'normal',
+                 z_dim: int = 10,
+                 z_dist: Literal['normal','laplacian','cauchy','studentt','gumbel'] = 'laplacian',
+                 loss_func: Literal['negbinomial','poisson','multinomial','bernoulli'] = 'multinomial',
+                 hidden_layers: list =[300],
+                 hidden_layer_activation: Literal['relu','softplus','leakyrelu','linear'] = 'relu',
+                 inverse_dispersion: float = 10.0,
+                 nn_dropout: float = 0.1,
+                 post_layer_fct: list = ['layernorm'],
+                 post_act_fct: list = None,
+                 config_enum: str = 'parallel',
+                 use_cuda: bool = False,
+                 seed: int = 42,
+                 dtype = torch.float32, # type: ignore
+                 ):
+        super().__init__()
+
+        # initialize the class with all arguments provided to the constructor
+        self.input_size = input_size
+        self.undesired_size = undesired_size
+        self.inverse_dispersion = inverse_dispersion
+        self.z_dim = z_dim
+        self.hidden_layers = hidden_layers
+        self.use_undesired = True if self.undesired_size>0 else False
+        self.allow_broadcast = config_enum == 'parallel'
+        self.use_cuda = use_cuda
+        self.loss_func = loss_func
+        self.options = None
+        self.code_size=codebook_size
+        self.D_size=d_dim
+        self.z_dist = z_dist
+        self.d_dist = d_dist
+        self.G = None
+        self.supervised_mode = supervised_mode
+        self.dtype = dtype
+        self.use_cell_factor = use_cell_factor
+        self.normalize = True
+
+        self.nn_dropout = nn_dropout
+        self.post_layer_fct = post_layer_fct
+        self.post_act_fct = post_act_fct
+        self.hidden_layer_activation = hidden_layer_activation
+
+        assert loss_func in ['poisson','multinomial','negbinomial','bernoulli']
+        
+        if seed is not None:
+            set_random_seed(seed)
+            
+        # define and instantiate the neural networks representing
+        # the parameters of various distributions in the model
+        self.setup_networks()
+
+    def setup_networks(self):
+        z_dim = self.z_dim
+        hidden_sizes = self.hidden_layers
+
+        nn_layer_norm, nn_batch_norm, nn_layer_dropout = False, False, False
+        na_layer_norm, na_batch_norm, na_layer_dropout = False, False, False
+
+        if self.post_layer_fct is not None:
+            nn_layer_norm=True if ('layernorm' in self.post_layer_fct) or ('layer_norm' in self.post_layer_fct) else False
+            nn_batch_norm=True if ('batchnorm' in self.post_layer_fct) or ('batch_norm' in self.post_layer_fct) else False
+            nn_layer_dropout=True if 'dropout' in self.post_layer_fct else False
+
+        if self.post_act_fct is not None:
+            na_layer_norm=True if ('layernorm' in self.post_act_fct) or ('layer_norm' in self.post_act_fct) else False
+            na_batch_norm=True if ('batchnorm' in self.post_act_fct) or ('batch_norm' in self.post_act_fct) else False
+            na_layer_dropout=True if 'dropout' in self.post_act_fct else False
+
+        if nn_layer_norm and nn_batch_norm and nn_layer_dropout:
+            post_layer_fct = lambda layer_ix, total_layers, layer: nn.Sequential(nn.Dropout(self.nn_dropout),nn.BatchNorm1d(layer.module.out_features), nn.LayerNorm(layer.module.out_features))
+        elif nn_layer_norm and nn_layer_dropout:
+            post_layer_fct = lambda layer_ix, total_layers, layer: nn.Sequential(nn.Dropout(self.nn_dropout), nn.LayerNorm(layer.module.out_features))
+        elif nn_batch_norm and nn_layer_dropout:
+            post_layer_fct = lambda layer_ix, total_layers, layer: nn.Sequential(nn.Dropout(self.nn_dropout), nn.BatchNorm1d(layer.module.out_features))
+        elif nn_layer_norm and nn_batch_norm:
+            post_layer_fct = lambda layer_ix, total_layers, layer: nn.Sequential(nn.BatchNorm1d(layer.module.out_features), nn.LayerNorm(layer.module.out_features))
+        elif nn_layer_norm:
+            post_layer_fct = lambda layer_ix, total_layers, layer: nn.LayerNorm(layer.module.out_features)
+        elif nn_batch_norm:
+            post_layer_fct = lambda layer_ix, total_layers, layer:nn.BatchNorm1d(layer.module.out_features)
+        elif nn_layer_dropout:
+            post_layer_fct = lambda layer_ix, total_layers, layer: nn.Dropout(self.nn_dropout)
+        else:
+            post_layer_fct = lambda layer_ix, total_layers, layer: None
+
+        if na_layer_norm and na_batch_norm and na_layer_dropout:
+            post_act_fct = lambda layer_ix, total_layers, layer: nn.Sequential(nn.Dropout(self.nn_dropout),nn.BatchNorm1d(layer.module.out_features), nn.LayerNorm(layer.module.out_features))
+        elif na_layer_norm and na_layer_dropout:
+            post_act_fct = lambda layer_ix, total_layers, layer: nn.Sequential(nn.Dropout(self.nn_dropout), nn.LayerNorm(layer.module.out_features))
+        elif na_batch_norm and na_layer_dropout:
+            post_act_fct = lambda layer_ix, total_layers, layer: nn.Sequential(nn.Dropout(self.nn_dropout), nn.BatchNorm1d(layer.module.out_features))
+        elif na_layer_norm and na_batch_norm:
+            post_act_fct = lambda layer_ix, total_layers, layer: nn.Sequential(nn.BatchNorm1d(layer.module.out_features), nn.LayerNorm(layer.module.out_features))
+        elif na_layer_norm:
+            post_act_fct = lambda layer_ix, total_layers, layer: nn.LayerNorm(layer.module.out_features)
+        elif na_batch_norm:
+            post_act_fct = lambda layer_ix, total_layers, layer:nn.BatchNorm1d(layer.module.out_features)
+        elif na_layer_dropout:
+            post_act_fct = lambda layer_ix, total_layers, layer: nn.Dropout(self.nn_dropout)
+        else:
+            post_act_fct = lambda layer_ix, total_layers, layer: None
+
+        if self.hidden_layer_activation == 'relu':
+            activate_fct = nn.ReLU
+        elif self.hidden_layer_activation == 'softplus':
+            activate_fct = nn.Softplus
+        elif self.hidden_layer_activation == 'leakyrelu':
+            activate_fct = nn.LeakyReLU
+        elif self.hidden_layer_activation == 'linear':
+            activate_fct = nn.Identity
+
+        # define the neural networks used later in the model and the guide.
+        if self.supervised_mode:
+            self.encoder_n = MLP(
+                [self.input_size] + hidden_sizes + [self.code_size],
+                activation=activate_fct,
+                output_activation=None,
+                post_layer_fct=post_layer_fct,
+                post_act_fct=post_act_fct,
+                allow_broadcast=self.allow_broadcast,
+                use_cuda=self.use_cuda,
+            )
+        else:
+            self.encoder_n = MLP(
+                [self.D_size] + hidden_sizes + [self.code_size],
+                activation=activate_fct,
+                output_activation=None,
+                post_layer_fct=post_layer_fct,
+                post_act_fct=post_act_fct,
+                allow_broadcast=self.allow_broadcast,
+                use_cuda=self.use_cuda,
+            )
+
+        self.encoder_zn = MLP(
+            [self.input_size] + hidden_sizes + [[z_dim, z_dim]],
+            activation=activate_fct,
+            output_activation=[None, Exp],
+            post_layer_fct=post_layer_fct,
+            post_act_fct=post_act_fct,
+            allow_broadcast=self.allow_broadcast,
+            use_cuda=self.use_cuda,
+        )
+
+        self.encoder_d = MLP(
+            [self.z_dim] + hidden_sizes + [[self.D_size, self.D_size]],
+            activation=activate_fct,
+            output_activation=[None, Exp],
+            post_layer_fct=post_layer_fct,
+            post_act_fct=post_act_fct,
+            allow_broadcast=self.allow_broadcast,
+            use_cuda=self.use_cuda,
+        )
+        
+        if self.use_cell_factor:
+            self.cell_factor = MLP(
+                [self.input_size] + hidden_sizes + [1],
+                activation=activate_fct,
+                output_activation=None,
+                post_layer_fct=post_layer_fct,
+                post_act_fct=post_act_fct,
+                allow_broadcast=self.allow_broadcast,
+                use_cuda=self.use_cuda,
+            )
+
+        if self.use_undesired:
+            self.decoder_concentrate = MLP(
+                [self.undesired_size + self.z_dim] + hidden_sizes + [self.input_size],
+                activation=activate_fct,
+                output_activation=None,
+                post_layer_fct=post_layer_fct,
+                post_act_fct=post_act_fct,
+                allow_broadcast=self.allow_broadcast,
+                use_cuda=self.use_cuda,
+            )
+        else:
+            self.decoder_concentrate = MLP(
+                [self.z_dim] + hidden_sizes + [self.input_size],
+                activation=activate_fct,
+                output_activation=None,
+                post_layer_fct=post_layer_fct,
+                post_act_fct=post_act_fct,
+                allow_broadcast=self.allow_broadcast,
+                use_cuda=self.use_cuda,
+            )
+
+        if self.d_dist == 'studentt':
+            self.codebook = MLP(
+                [self.code_size] + hidden_sizes + [[self.D_size,self.D_size]],
+                activation=activate_fct,
+                output_activation=[Exp, None],
+                post_layer_fct=post_layer_fct,
+                post_act_fct=post_act_fct,
+                allow_broadcast=self.allow_broadcast,
+                use_cuda=self.use_cuda,
+            )
+        else:
+            self.codebook = MLP(
+                [self.code_size] + hidden_sizes + [self.D_size],
+                activation=activate_fct,
+                output_activation=None,
+                post_layer_fct=post_layer_fct,
+                post_act_fct=post_act_fct,
+                allow_broadcast=self.allow_broadcast,
+                use_cuda=self.use_cuda,
+            )
+
+        if self.z_dist == 'studentt':
+            self.decoder_zn = MLP(
+                [self.D_size] + hidden_sizes + [[self.z_dim,self.z_dim]],
+                activation=activate_fct,
+                output_activation=[Exp,None],
+                post_layer_fct=post_layer_fct,
+                post_act_fct=post_act_fct,
+                allow_broadcast=self.allow_broadcast,
+                use_cuda=self.use_cuda,
+            )
+        else:
+            self.decoder_zn = MLP(
+                [self.D_size] + hidden_sizes + [self.z_dim],
+                activation=activate_fct,
+                output_activation=None,
+                post_layer_fct=post_layer_fct,
+                post_act_fct=post_act_fct,
+                allow_broadcast=self.allow_broadcast,
+                use_cuda=self.use_cuda,
+            )
+
+        # using GPUs for faster training of the networks
+        if self.use_cuda:
+            self.cuda()
+
+    def cutoff(self, xs, thresh=None):
+        eps = torch.finfo(xs.dtype).eps
+        
+        if not thresh is None:
+            if eps < thresh:
+                eps = thresh
+
+        xs = xs.clamp(min=eps)
+
+        if torch.any(torch.isnan(xs)):
+            xs[torch.isnan(xs)] = eps
+
+        return xs
+
+    def softmax(self, xs):
+        #soft_enc = nn.Softmax(dim=1)
+        #xs = soft_enc(xs)
+        #xs = clamp_probs(xs)
+        #xs = ft.normalize(xs, 1, 1)
+        xs = SoftmaxTransform()(xs)
+        return xs
+    
+    def sigmoid(self, xs):
+        sigm_enc = nn.Sigmoid()
+        xs = sigm_enc(xs)
+        xs = clamp_probs(xs)
+        return xs
+
+    def softmax_logit(self, xs):
+        eps = torch.finfo(xs.dtype).eps
+        xs = self.softmax(xs)
+        xs = torch.logit(xs, eps=eps)
+        return xs
+
+    def logit(self, xs):
+        eps = torch.finfo(xs.dtype).eps
+        xs = torch.logit(xs, eps=eps)
+        return xs
+
+    def dirimulti_param(self, xs):
+        xs = self.dirimulti_mass * self.sigmoid(xs)
+        return xs
+
+    def multi_param(self, xs):
+        xs = self.softmax(xs)
+        return xs
+    
+    def get_device(self):
+        return next(self.parameters()).device
+
+    def model(self, xs, embeds=None, ks2=None):
+        # register this pytorch module and all of its sub-modules with pyro
+        pyro.module('HMAP', self)
+
+        eps = torch.finfo(xs.dtype).eps
+        batch_size = xs.size(0)
+        self.options = dict(dtype=xs.dtype, device=xs.device)
+
+        if self.loss_func == 'negbinomial':
+            total_count = pyro.param("inverse_dispersion", self.inverse_dispersion * torch.ones(1, self.input_size, **self.options),
+                                     constraint=constraints.positive)
+            
+        acs_scale = pyro.param("codebook_scale", torch.ones(1, self.D_size, **self.options),
+                                 constraint=constraints.positive)
+        zn_scale = pyro.param("z_scale", torch.ones(1, self.z_dim, **self.options),
+                                 constraint=constraints.positive)
+        
+        I = torch.eye(self.code_size)
+        if self.d_dist == 'studentt':
+            acs_dof,acs_loc = self.codebook(I)
+        else:
+            acs_loc = self.codebook(I)
+
+        with pyro.plate('data'):
+            ###############################################
+            # p(zn)
+            prior = torch.zeros(batch_size, self.code_size, **self.options)
+            ns = pyro.sample('n', dist.OneHotCategorical(logits=prior))
+
+            if self.d_dist == 'studentt':
+                d_dof = torch.matmul(ns,acs_dof)
+            d_loc = torch.matmul(ns,acs_loc)
+            d_scale = acs_scale
+            if self.d_dist == 'normal':
+                ds = pyro.sample('d', dist.Normal(d_loc, d_scale).to_event(1))
+            elif self.d_dist == 'laplacian':
+                ds = pyro.sample('d', dist.Laplace(d_loc, d_scale).to_event(1))
+            elif self.d_dist == 'cauchy':
+                ds = pyro.sample('d', dist.Cauchy(d_loc, d_scale).to_event(1))
+            elif self.d_dist == 'vonmises':
+                ds = pyro.sample('d', dist.VonMises(d_loc, d_scale).to_event(1))
+            elif self.d_dist == 'gumbel':
+                ds = pyro.sample('d', dist.Gumbel(d_loc, d_scale).to_event(1))
+            elif self.d_dist == 'studentt':
+                ds = pyro.sample('d', dist.StudentT(df=d_dof, loc=d_loc, scale=d_scale).to_event(1))
+
+            if self.z_dist == 'studentt':
+                zn_dof,zn_loc = self.decoder_zn(ds)
+            else:
+                zn_loc = self.decoder_zn(ds)
+            if self.z_dist == 'laplacian':
+                if embeds is None:
+                    zns = pyro.sample('zn', dist.Laplace(zn_loc, zn_scale).to_event(1))
+                else:
+                    zns = pyro.sample('zn', dist.Laplace(zn_loc, zn_scale).to_event(1), obs=embeds)
+            elif self.z_dist == 'cauchy':
+                if embeds is None:
+                    zns = pyro.sample('zn', dist.Cauchy(zn_loc, zn_scale).to_event(1))
+                else:
+                    zns = pyro.sample('zn', dist.Cauchy(zn_loc, zn_scale).to_event(1), obs=embeds)
+            elif self.z_dist == 'normal':
+                if embeds is None:
+                    zns = pyro.sample('zn', dist.Normal(zn_loc, zn_scale).to_event(1))
+                else:
+                    zns = pyro.sample('zn', dist.Normal(zn_loc, zn_scale).to_event(1), obs=embeds)
+            elif self.z_dist == 'studentt':
+                if embeds is None:
+                    zns = pyro.sample('zn', dist.StudentT(df=zn_dof, loc=zn_loc, scale=zn_scale).to_event(1))
+                else:
+                    zns = pyro.sample('zn', dist.StudentT(df=zn_dof, loc=zn_loc, scale=zn_scale).to_event(1), obs=embeds)
+            elif self.z_dist == 'gumbel':
+                if embeds is None:
+                    zns = pyro.sample('zn', dist.Gumbel(zn_loc, zn_scale).to_event(1))
+                else:
+                    zns = pyro.sample('zn', dist.Gumbel(zn_loc, zn_scale).to_event(1), obs=embeds)
+
+            ###############################################
+            # p(a | zys, zk2s)
+            if self.use_undesired:
+                zs = [ks2, zns]
+            else:
+                zs = zns
+
+            concentrate = self.decoder_concentrate(zs)
+            if self.use_cell_factor:
+                cf = self.cell_factor(xs)
+                concentrate += cf 
+                
+            if self.normalize:
+                rate = concentrate.exp()
+                if self.loss_func != 'poisson':
+                    theta = dist.DirichletMultinomial(total_count=1, concentration=rate).mean
+            else:
+                if self.loss_func == 'poisson':
+                    rate = concentrate.exp()
+                else:
+                    logits = concentrate
+
+            if self.loss_func == 'negbinomial':
+                if self.normalize:
+                    pyro.sample('x', dist.NegativeBinomial(total_count=total_count, probs=theta).to_event(1), obs=xs)
+                else:
+                     pyro.sample('x', dist.NegativeBinomial(total_count=total_count, logits=logits).to_event(1), obs=xs)
+            elif self.loss_func == 'multinomial':
+                if self.normalize:
+                    pyro.sample('x', dist.Multinomial(total_count=int(1e8), probs=theta), obs=xs)
+                else:
+                    pyro.sample('x', dist.Multinomial(total_count=int(1e8), logits=logits), obs=xs)
+            elif self.loss_func == 'bernoulli':
+                if self.normalize:
+                    pyro.sample('x', dist.Bernoulli(probs=theta).to_event(1), obs=xs)
+                else:
+                    pyro.sample('x', dist.Bernoulli(logits=logits).to_event(1), obs=xs)
+            elif self.loss_func == 'poisson':
+                pyro.sample('x', dist.Poisson(rate=rate).to_event(1), obs=xs.round())
+
+    def model2(self, xs, ys=None, embeds=None, ks2=None):
+        # register this pytorch module and all of its sub-modules with pyro
+        pyro.module('HMAP', self)
+
+        eps = torch.finfo(xs.dtype).eps
+        batch_size = xs.size(0)
+        self.options = dict(dtype=xs.dtype, device=xs.device)
+
+        if self.loss_func == 'negbinomial':
+            total_count = pyro.param("inverse_dispersion", self.inverse_dispersion * torch.ones(1, self.input_size, **self.options),
+                                     constraint=constraints.positive)
+        acs_scale = pyro.param("codebook_scale", torch.ones(1, self.D_size, **self.options),
+                                 constraint=constraints.positive)
+        zn_scale = pyro.param("z_scale", torch.ones(1, self.z_dim, **self.options),
+                                 constraint=constraints.positive)
+        
+        I = torch.eye(self.code_size)
+        if self.d_dist == 'studentt':
+            acs_dof,acs_loc = self.codebook(I)
+        else:
+            acs_loc = self.codebook(I)
+
+        with pyro.plate('data'):
+            ###############################################
+            # p(zn)
+            if ys is None:
+                prior = torch.zeros(batch_size, self.code_size, **self.options)
+                ns = pyro.sample('n', dist.OneHotCategorical(logits=prior))
+            else:
+                prior = self.encoder_n(xs)
+                ns = pyro.sample('n', dist.OneHotCategorical(logits=prior), obs=ys)
+
+            if self.d_dist == 'studentt':
+                d_dof = torch.matmul(ns,acs_dof)
+            d_loc = torch.matmul(ns,acs_loc)
+            d_scale = acs_scale
+            if self.d_dist == 'normal':
+                ds = pyro.sample('d', dist.Normal(d_loc, d_scale).to_event(1))
+            elif self.d_dist == 'laplacian':
+                ds = pyro.sample('d', dist.Laplace(d_loc, d_scale).to_event(1))
+            elif self.d_dist == 'cauchy':
+                ds = pyro.sample('d', dist.Cauchy(d_loc, d_scale).to_event(1))
+            elif self.d_dist == 'vonmises':
+                ds = pyro.sample('d', dist.VonMises(d_loc, d_scale).to_event(1))
+            elif self.d_dist == 'gumbel':
+                ds = pyro.sample('d', dist.Gumbel(d_loc, d_scale).to_event(1))
+            elif self.d_dist == 'studentt':
+                ds = pyro.sample('d', dist.StudentT(df=d_dof, loc=d_loc, scale=d_scale).to_event(1))
+
+            if self.z_dist == 'studentt':
+                zn_dof,zn_loc = self.decoder_zn(ds)
+            else:
+                zn_loc = self.decoder_zn(ds)
+            if self.z_dist == 'laplacian':
+                if embeds is None:
+                    zns = pyro.sample('zn', dist.Laplace(zn_loc, zn_scale).to_event(1))
+                else:
+                    zns = pyro.sample('zn', dist.Laplace(zn_loc, zn_scale).to_event(1), obs=embeds)
+            elif self.z_dist == 'cauchy':
+                if embeds is None:
+                    zns = pyro.sample('zn', dist.Cauchy(zn_loc, zn_scale).to_event(1))
+                else:
+                    zns = pyro.sample('zn', dist.Cauchy(zn_loc, zn_scale).to_event(1), obs=embeds)
+            elif self.z_dist == 'normal':
+                if embeds is None:
+                    zns = pyro.sample('zn', dist.Normal(zn_loc, zn_scale).to_event(1))
+                else:
+                    zns = pyro.sample('zn', dist.Normal(zn_loc, zn_scale).to_event(1), obs=embeds)
+            elif self.z_dist == 'studentt':
+                if embeds is None:
+                    zns = pyro.sample('zn', dist.StudentT(df=zn_dof, loc=zn_loc, scale=zn_scale).to_event(1))
+                else:
+                    zns = pyro.sample('zn', dist.StudentT(df=zn_dof, loc=zn_loc, scale=zn_scale).to_event(1), obs=embeds)
+            elif self.z_dist == 'gumbel':
+                if embeds is None:
+                    zns = pyro.sample('zn', dist.Gumbel(zn_loc, zn_scale).to_event(1))
+                else:
+                    zns = pyro.sample('zn', dist.Gumbel(zn_loc, zn_scale).to_event(1), obs=embeds)
+
+            ###############################################
+            # p(a | zys, zk2s)
+            if self.use_undesired:
+                zs = [ks2, zns]
+            else:
+                zs = zns
+
+            concentrate = self.decoder_concentrate(zs)
+            if self.use_cell_factor:
+                cf = self.cell_factor(xs)
+                concentrate += cf 
+                
+            if self.normalize:
+                rate = concentrate.exp()
+                if self.loss_func != 'poisson':
+                    theta = dist.DirichletMultinomial(total_count=1, concentration=rate).mean
+            else:
+                if self.loss_func == 'poisson':
+                    rate = concentrate.exp()
+                else:
+                    logits = concentrate
+
+            if self.loss_func == 'negbinomial':
+                if self.normalize:
+                    pyro.sample('x', dist.NegativeBinomial(total_count=total_count, probs=theta).to_event(1), obs=xs)
+                else:
+                     pyro.sample('x', dist.NegativeBinomial(total_count=total_count, logits=logits).to_event(1), obs=xs)
+            elif self.loss_func == 'multinomial':
+                if self.normalize:
+                    pyro.sample('x', dist.Multinomial(total_count=int(1e8), probs=theta), obs=xs)
+                else:
+                    pyro.sample('x', dist.Multinomial(total_count=int(1e8), logits=logits), obs=xs)
+            elif self.loss_func == 'bernoulli':
+                if self.normalize:
+                    pyro.sample('x', dist.Bernoulli(probs=theta).to_event(1), obs=xs)
+                else:
+                    pyro.sample('x', dist.Bernoulli(logits=logits).to_event(1), obs=xs)
+            elif self.loss_func == 'poisson':
+                pyro.sample('x', dist.Poisson(rate=rate).to_event(1), obs=xs.round())
+
+    def guide(self, xs, embeds=None, ks2=None):
+        # inform Pyro that the variables in the batch of xs, ys are conditionally independent
+        with pyro.plate('data'):
+            # q(zn | x)
+            if embeds is None:
+                zn_loc, zn_scale = self.encoder_zn(xs)
+                zns = pyro.sample('zn', dist.Normal(zn_loc, zn_scale).to_event(1))
+            else:
+                zns = embeds
+            
+            d_loc,d_scale = self.encoder_d(zns)
+            ds = pyro.sample('d', dist.Normal(d_loc, d_scale).to_event(1))
+            
+            alpha = self.encoder_n(ds)
+            ns = pyro.sample('n', dist.OneHotCategorical(logits=alpha))
+
+    def guide2(self, xs, ys=None, embeds=None, ks2=None):
+        # inform Pyro that the variables in the batch of xs, ys are conditionally independent
+        with pyro.plate('data'):
+            # q(zn | x)
+            if embeds is None:
+                zn_loc, zn_scale = self.encoder_zn(xs)
+                zns = pyro.sample('zn', dist.Normal(zn_loc, zn_scale).to_event(1))
+            else:
+                zns = embeds
+
+            d_loc,d_scale = self.encoder_d(zns)
+            ds = pyro.sample('d', dist.Normal(d_loc, d_scale).to_event(1))
+            
+            if ys is None:
+                alpha = self.encoder_n(ds)
+                ns = pyro.sample('n', dist.OneHotCategorical(logits=alpha))
+
+    def _codebook(self):
+        I = torch.eye(self.code_size, **self.options)
+        if self.d_dist == 'studentt':
+            _,loc = self.codebook(I)
+        else:
+            loc = self.codebook(I)
+        return loc
+    
+    def get_codebook(self):
+        I = torch.eye(self.code_size, **self.options)
+        if self.d_dist == 'studentt':
+            _,cb = self.codebook(I)
+        else:
+            cb = self.codebook(I)
+
+        if self.z_dist == 'studentt':
+            _,zs = self.decoder_zn(cb)
+        else:
+            zs = self.decoder_zn(cb)
+        return tensor_to_numpy(cb), tensor_to_numpy(zs)
+    
+    def _code(self, xs):
+        if self.supervised_mode:
+            alpha = self.encoder_n(xs)
+        else:
+            zns,_ = self.encoder_zn(xs)
+            ds,_ = self.encoder_d(zns)
+            alpha = self.encoder_n(ds)
+        return alpha
+    
+    def _l0_embedding(self,xs):
+        ns = self._soft_assignments(xs)
+        acs = self._codebook()
+        return torch.matmul(ns, acs)
+    
+    def _l1_embedding(self,xs,zs=None):
+        if zs is None:
+            zs = self._l2_embedding(xs)
+        ds,_ = self.encoder_d(zs)
+        return ds
+    
+    def _l2_embedding(self, xs):
+        zns, _ = self.encoder_zn(xs)
+        return zns
+    
+    def get_l0_embedding(self, xs, batch_size=1024):
+        xs = convert_to_tensor(xs, device=self.get_device())
+        dataset = CustomDataset(xs)
+        dataloader = DataLoader(dataset, batch_size=batch_size, shuffle=False)
+
+        Z = []
+        with tqdm(total=len(dataloader), desc='', unit='batch') as pbar:
+            for X_batch, _ in dataloader:
+                zns = self._l0_embedding(X_batch)
+                Z.append(tensor_to_numpy(zns))
+                pbar.update(1)
+
+        Z = np.concatenate(Z)
+        return Z
+    
+    def get_l1_embedding(self, xs, zs=None, batch_size=1024):
+        xs = convert_to_tensor(xs, device=self.get_device())
+        if zs is not None:
+            zs = convert_to_tensor(zs, device=self.get_device())
+        dataset = CustomDataset2(xs, zs)
+        dataloader = DataLoader(dataset, batch_size=batch_size, shuffle=False)
+
+        Z = []
+        with tqdm(total=len(dataloader), desc='', unit='batch') as pbar:
+            for X_batch, Z_batch, _ in dataloader:
+                if zs is None:
+                    Z_batch = None
+                zns = self._l1_embedding(X_batch, zs=Z_batch)
+                Z.append(tensor_to_numpy(zns))
+                pbar.update(1)
+
+        Z = np.concatenate(Z)
+        return Z
+    
+    def get_l2_embedding(self,xs,batch_size=1024):
+        xs = convert_to_tensor(xs, device=self.get_device())
+        dataset = CustomDataset(xs)
+        dataloader = DataLoader(dataset, batch_size=batch_size, shuffle=False)
+
+        Z = []
+        with tqdm(total=len(dataloader), desc='', unit='batch') as pbar:
+            for X_batch, _ in dataloader:
+                zns = self._l2_embedding(X_batch)
+                Z.append(tensor_to_numpy(zns))
+                pbar.update(1)
+
+        Z = np.concatenate(Z)
+        return Z
+    
+    def _soft_assignments(self, xs):
+        alpha = self._code(xs)
+        alpha = self.softmax(alpha)
+        return alpha
+       
+    def soft_assignments(self, xs, batch_size=1024):
+        xs = convert_to_tensor(xs, device=self.get_device())
+        dataset = CustomDataset(xs)
+        dataloader = DataLoader(dataset, batch_size=batch_size, shuffle=False)
+
+        A = []
+        with tqdm(total=len(dataloader), desc='', unit='batch') as pbar:
+            for X_batch, _ in dataloader:
+                a = self._soft_assignments(X_batch)
+                A.append(tensor_to_numpy(a))
+                pbar.update(1)
+
+        A = np.concatenate(A)
+        return A
+    
+    def _hard_assignments(self, xs):
+        alpha = self._code(xs)
+        res, ind = torch.topk(alpha, 1)
+        ns = torch.zeros_like(alpha).scatter_(1, ind, 1.0)
+        return ns
+    
+    def hard_assignments(self, xs, batch_size=1024):
+        xs = convert_to_tensor(xs, device=self.get_device())
+        dataset = CustomDataset(xs)
+        dataloader = DataLoader(dataset, batch_size=batch_size, shuffle=False)
+
+        A = []
+        with tqdm(total=len(dataloader), desc='', unit='batch') as pbar:
+            for X_batch, _ in dataloader:
+                a = self._hard_assignments(X_batch)
+                A.append(tensor_to_numpy(a))
+                pbar.update(1)
+
+        A = np.concatenate(A)
+        return A
+    
+    def mmd_gaussian(self, x, y, sigma=None):
+        """
+        Compute MMD with Gaussian kernel between samples x and y.
+    
+        Args:
+            x: Tensor of shape (n_samples, n_features)
+            y: Tensor of shape (m_samples, n_features)
+            sigma: Bandwidth of the Gaussian kernel. If None, uses median heuristic.
+    
+        Returns:
+            mmd: Squared MMD value.
+        """
+        n, m = x.size(0), y.size(0)
+    
+        if sigma is None:
+            # Median heuristic for bandwidth
+            xy = torch.cat([x, y], dim=0)
+            pairwise_dist = torch.cdist(xy, xy, p=2)
+            sigma = torch.median(pairwise_dist[pairwise_dist > 0]).detach()
+    
+        # Kernel matrices
+        xx = torch.exp(-torch.cdist(x, x, p=2)**2 / (2 * sigma**2))
+        yy = torch.exp(-torch.cdist(y, y, p=2)**2 / (2 * sigma**2))
+        xy = torch.exp(-torch.cdist(x, y, p=2)**2 / (2 * sigma**2))
+    
+        # Compute MMD
+        mmd = (xx.sum() - xx.diag().sum()) / (n * (n - 1)) + \
+              (yy.sum() - yy.diag().sum()) / (m * (m - 1)) - \
+            2 * xy.mean()
+        return mmd
+
+    def sinkhorn_distance(self, x, y, epsilon=0.01, max_iters=100):
+        """
+        Compute regularized Wasserstein distance using Sinkhorn algorithm.
+    
+        Args:
+            x: Tensor of shape (n, d)
+            y: Tensor of shape (m, d)
+            epsilon: Regularization parameter
+            max_iters: Number of Sinkhorn iterations
+    
+        Returns:
+            wasserstein: Approximated Wasserstein distance.
+        """
+        n, m = x.size(0), y.size(0)
+        C = torch.cdist(x, y, p=2)**2  # Cost matrix
+    
+        # Initialize dual variables
+        u, v = torch.zeros(n, device=x.device), torch.zeros(m, device=y.device)
+    
+        for _ in range(max_iters):
+            u = (torch.logsumexp((C - v[None, :]) / epsilon, dim=1)) / (1 / epsilon)
+            v = (torch.logsumexp((C - u[:, None]) / epsilon, dim=0)) / (1 / epsilon)
+    
+        # Compute transport plan and distance
+        P = torch.exp((u[:, None] + v[None, :] - C) / epsilon)
+        return torch.sum(P * C)
+
+    def metacell_distance(self, xs, ys=None, metric:Literal['mmd','sinkhorn']='mmd', epsilon:float=0.01, max_iters:int=100):
+        n_metacells = ys.shape[1]
+        dm2m = torch.zeros(n_metacells, n_metacells)
+        mc = np.argmax(ys, axis=1)
+
+        xs = convert_to_tensor(xs, device=self.get_device())
+        combinations = itertools.product(np.arange(n_metacells), np.arange(n_metacells))
+        for i,j in combinations:
+            i_cells = np.where(mc==i)
+            j_cells = np.where(mc==j)
+            if metric=='mmd':
+                dm2m[i,j] = self.mmd_gaussian(xs[i_cells],xs[j_cells])
+            else:
+                dm2m[i,j] = self.sinkhorn_distance(xs[i_cells],xs[j_cells],epsilon=epsilon,max_iters=max_iters)
+
+        return tensor_to_numpy(dm2m)
+    
+    def metacell_similarity(self, xs, ys=None, embed: Literal['l1','l2']='l1', n_neighbors=50, sigma=1, use_diffuse=False, diffusion_time=1):
+        if ys is None:
+            ys = self.soft_assignments(xs)
+        if not use_diffuse:
+            ys = convert_to_tensor(ys, device=self.get_device())
+            m2m = torch.matmul(ys.T / torch.sum(ys.T, dim=1, keepdim=True), ys)
+            m2m = tensor_to_numpy(m2m)
+        else:
+            if embed=='l1':
+                zs = self.get_l1_embedding(xs)
+            else:
+                zs = self.get_l2_embedding(xs)
+            m2m = compute_metacell_diffusion_kernel(zs, ys, n_neighbors=n_neighbors, sigma=sigma, diffusion_time=diffusion_time)
+        return m2m
+    
+    def metacell_network(self, affinity_matrix,
+                         #xs, ys=None, 
+                         #k=10, 
+                         exclude_metacells: list = None):
+        #affinity_matrix = self.metacell_similarity(xs, ys, use_diffuse=use_diffuse)
+        self.G = nx.Graph()
+        self.G.add_nodes_from(np.arange(self.code_size))
+        
+        #if k < affinity_matrix.shape[1]:
+        if True:
+            for i in np.arange(self.code_size):
+                arr = affinity_matrix[i,:]
+                #kth_largest_value = np.partition(arr, -k)[-k]
+                #arr[arr<kth_largest_value] = 0
+                #affinity_matrix[i,:] = arr
+
+                if exclude_metacells is None:
+                    for j in np.arange(len(arr)):
+                        if (arr[j]>0) and (j!=i):
+                            self.G.add_edge(i,j,weight=1/arr[j])
+                elif not (i in exclude_metacells):
+                    for j in np.arange(len(arr)):
+                        if (arr[j]>0) and (j!=i) and (not j in exclude_metacells):
+                            self.G.add_edge(i,j,weight=1/arr[j])
+            
+        return self.G
+    
+    def metacell_fa2(self, G, max_iter=100):
+        return visualize_metacell_igraph_with_fa2(G, iterations=max_iter)
+    
+    def metacell_tree(self, G, root_metacell=0):
+        T = nx.minimum_spanning_tree(G)
+        sorted(T.edges(data=True))
+        tree = nx.dfs_tree(T, root_metacell)
+
+        return tree
+    
+    def fit(self, xs, 
+            ys = None,
+            zs = None,
+            us = None, 
+            num_epochs: int = 200, 
+            learning_rate: float = 0.0001, 
+            batch_size: int = 512, 
+            algo: Literal['adam','rmsprop','adamw'] = 'adam', 
+            beta_1: float = 0.9, 
+            weight_decay: float = 0.005, 
+            decay_rate: float = 0.9,
+            threshold: int = 0,
+            normalize: bool = True,
+            config_enum: str = 'parallel',
+            use_jax: bool = False):
+        """
+        Train the SURE model.
+
+        Parameters
+        ----------
+        xs
+            Single-cell experssion matrix. It should be a Numpy array or a Pytorch Tensor. Rows are cells and columns are features.
+        us
+            Undesired factor matrix. It should be a Numpy array or a Pytorch Tensor. Rows are cells and columns are undesired factors.
+        num_epochs
+            Number of training epochs.
+        learning_rate
+            Parameter for training.
+        batch_size
+            Size of batch processing.
+        algo
+            Optimization algorithm.
+        beta_1
+            Parameter for optimization.
+        weight_decay
+            Parameter for optimization.
+        decay_rate 
+            Parameter for optimization.
+        use_jax
+            If toggled on, Jax will be used for speeding up. CAUTION: This will raise errors because of unknown reasons when it is called in
+            the Python script or Jupyter notebook. It is OK if it is used when runing SURE in the shell command.
+        """
+        self.normalize = normalize
+        
+        if self.loss_func == 'bernoulli':
+            ad = sc.AnnData(xs)
+            binarize(ad, threshold=threshold)
+            xs = ad.X.copy()
+        else:
+            xs = np.round(xs)
+            
+        if sparse.issparse(xs):
+            xs = xs.toarray()
+            
+        xs = convert_to_tensor(xs, dtype=self.dtype, device=self.get_device())
+        if ys is not None:
+            ys = convert_to_tensor(ys, dtype=self.dtype, device=self.get_device())
+        if zs is not None:
+            zs = convert_to_tensor(zs, dtype=self.dtype, device=self.get_device())
+        if us is not None:
+            us = convert_to_tensor(us, dtype=self.dtype, device=self.get_device())
+        else:
+            self.use_undesired = False
+        self.options = dict(dtype=xs.dtype, device=xs.device)
+
+        dataset = CustomDataset4(xs, ys, zs, us)
+        dataloader = DataLoader(dataset, batch_size=batch_size, shuffle=True)
+
+        # setup the optimizer
+        optim_params = {'lr': learning_rate, 'betas': (beta_1, 0.999), 'weight_decay': weight_decay}
+
+        if algo.lower()=='rmsprop':
+            optimizer = torch.optim.RMSprop
+        elif algo.lower()=='adam':
+            optimizer = torch.optim.Adam
+        elif algo.lower() == 'adamw':
+            optimizer = torch.optim.AdamW
+        else:
+            raise ValueError("An optimization algorithm must be specified.")
+        scheduler = ExponentialLR({'optimizer': optimizer, 'optim_args': optim_params, 'gamma': decay_rate})
+
+        pyro.clear_param_store()
+
+        # set up the loss(es) for inference, wrapping the guide in config_enumerate builds the loss as a sum
+        # by enumerating each class label form the sampled discrete categorical distribution in the model
+        Elbo = JitTraceEnum_ELBO if use_jax else TraceEnum_ELBO
+        elbo = Elbo(max_plate_nesting=1, strict_enumeration_warning=False)
+        if ys is None:
+            guide = config_enumerate(self.guide, config_enum, expand=True)
+            loss_basic = SVI(self.model, guide, scheduler, loss=elbo)
+        else:
+            guide = config_enumerate(self.guide2, config_enum, expand=True)
+            loss_basic = SVI(self.model2, guide, scheduler, loss=elbo)
+
+        # build a list of all losses considered
+        losses = [loss_basic]
+        num_losses = len(losses)
+
+        with tqdm(total=num_epochs, desc='Training', unit='epoch') as pbar:
+            for epoch in range(num_epochs):
+                epoch_losses = [0.0] * num_losses
+                for batch_x, batch_y, batch_z, batch_u, _ in dataloader:
+                    if us is None:
+                        batch_u = None
+                    if ys is None:
+                        batch_y = None
+                    if zs is None:
+                        batch_z = None
+
+                    for loss_id in range(num_losses):
+                        if batch_y is None:
+                            new_loss = losses[loss_id].step(batch_x, batch_z, batch_u)
+                            epoch_losses[loss_id] += new_loss
+                        else:
+                            new_loss = losses[loss_id].step(batch_x, batch_y, batch_z, batch_u)
+                            epoch_losses[loss_id] += new_loss
+
+                avg_epoch_losses_ = map(lambda v: v / len(dataloader), epoch_losses)
+                avg_epoch_losses = map(lambda v: "{:.4f}".format(v), avg_epoch_losses_)
+
+                # store the loss
+                str_loss = " ".join(map(str, avg_epoch_losses))
+
+                # Update progress bar
+                pbar.set_postfix({'loss': str_loss})
+                pbar.update(1)
+        
+        assigns = self.soft_assignments(xs)
+        assigns = convert_to_tensor(assigns, dtype=self.dtype, device=self.get_device())
+        self.codebook_weights = torch.sum(assigns, dim=0)
+        self.codebook_weights = self.codebook_weights / torch.sum(self.codebook_weights)
+
+    @classmethod
+    def save_model(cls, model, file_path, compression=False):
+        """Save the model to the specified file path."""
+        file_path = os.path.abspath(file_path)
+
+        model.eval()
+        if compression:
+            with gzip.open(file_path, 'wb') as pickle_file:
+                pickle.dump(model, pickle_file)
+        else:
+            with open(file_path, 'wb') as pickle_file:
+                pickle.dump(model, pickle_file)
+
+        print(f'Model saved to {file_path}')
+
+    @classmethod
+    def load_model(cls, file_path):
+        """Load the model from the specified file path and return an instance."""
+        print(f'Model loaded from {file_path}')
+
+        file_path = os.path.abspath(file_path)
+        if file_path.endswith('gz'):
+            with gzip.open(file_path, 'rb') as pickle_file:
+                model = pickle.load(pickle_file)
+        else:
+            with open(file_path, 'rb') as pickle_file:
+                model = pickle.load(pickle_file)
+        
+        return model
+
+
+EXAMPLE_RUN = (
+    "example run: HMAP --help"
+)
+
+def parse_args():
+    parser = argparse.ArgumentParser(
+        description="HMAP\n{}".format(EXAMPLE_RUN))
+
+    parser.add_argument(
+        "--cuda", action="store_true", help="use GPU(s) to speed up training"
+    )
+    parser.add_argument(
+        "--jit", action="store_true", help="use PyTorch jit to speed up training"
+    )
+    parser.add_argument(
+        "-n", "--num-epochs", default=200, type=int, help="number of epochs to run"
+    )
+    parser.add_argument(
+        "-enum",
+        "--enum-discrete",
+        default="parallel",
+        help="parallel, sequential or none. uses parallel enumeration by default",
+    )
+    parser.add_argument(
+        "-data",
+        "--data-file",
+        default=None,
+        type=str,
+        help="the data file",
+    )
+    parser.add_argument(
+        "-undesired",
+        "--undesired-factor-file",
+        default=None,
+        type=str,
+        help="the file for the record of undesired factors",
+    )
+    parser.add_argument(
+        "-64",
+        "--float64",
+        action="store_true",
+        help="use double float precision",
+    )
+    parser.add_argument(
+        "--z-dist",
+        default='studentt',
+        type=str,
+        choices=['normal','laplacian','cauchy','studentt','gumbel'],
+        help="distribution model for latent representation",
+    )
+    parser.add_argument(
+        "-zd",
+        "--z-dim",
+        default=10,
+        type=int,
+        help="size of the tensor representing the latent variable z",
+    )
+    parser.add_argument(
+        "-cs",
+        "--codebook_size",
+        default=30,
+        type=int,
+        help="size of vector quantization codebook",
+    )
+    parser.add_argument(
+        "-dd",
+        "--d-dim",
+        default=2,
+        type=int,
+        choices=[2,3],
+        help="size of the vector quantization codeword",
+    )
+    parser.add_argument(
+        "--d-dist",
+        default='normal',
+        type=str,
+        choices=['normal','laplacian','cauchy','vonmises','gumbel','studentt'],
+        help="distribution model for visual representation",
+    )
+    parser.add_argument(
+        "-hl",
+        "--hidden-layers",
+        nargs="+",
+        default=[300],
+        type=int,
+        help="a tuple (or list) of MLP layers to be used in the neural networks "
+        "representing the parameters of the distributions in our model",
+    )
+    parser.add_argument(
+        "-hla",
+        "--hidden-layer-activation",
+        default='relu',
+        type=str,
+        choices=['relu','softplus','leakyrelu','linear'],
+        help="activation function for hidden layers",
+    )
+    parser.add_argument(
+        "-plf",
+        "--post-layer-function",
+        nargs="+",
+        default=['layernorm'],
+        type=str,
+        help="post functions for hidden layers, could be none, dropout, layernorm, batchnorm, or combination, default is 'dropout layernorm'",
+    )
+    parser.add_argument(
+        "-paf",
+        "--post-activation-function",
+        nargs="+",
+        default=['none'],
+        type=str,
+        help="post functions for activation layers, could be none or dropout, default is 'none'",
+    )
+    parser.add_argument(
+        "-id",
+        "--inverse-dispersion",
+        default=10.0,
+        type=float,
+        help="inverse dispersion prior for negative binomial",
+    )
+    parser.add_argument(
+        "-lr",
+        "--learning-rate",
+        default=0.0001,
+        type=float,
+        help="learning rate for Adam optimizer",
+    )
+    parser.add_argument(
+        "-dr",
+        "--decay-rate",
+        default=0.9,
+        type=float,
+        help="decay rate for Adam optimizer",
+    )
+    parser.add_argument(
+        "--layer-dropout-rate",
+        default=0.1,
+        type=float,
+        help="droput rate for neural networks",
+    )
+    parser.add_argument(
+        "-b1",
+        "--beta-1",
+        default=0.95,
+        type=float,
+        help="beta-1 parameter for Adam optimizer",
+    )
+    parser.add_argument(
+        "-bs",
+        "--batch-size",
+        default=1000,
+        type=int,
+        help="number of cells to be considered in a batch",
+    )
+    parser.add_argument(
+        "-likeli",
+        "--likelihood",
+        default='poisson',
+        type=str,
+        choices=['negbinomial', 'multinomial', 'poisson'],
+        help="specify the distribution likelihood function",
+    )
+    parser.add_argument(
+        "--seed",
+        default=None,
+        type=int,
+        help="seed for controlling randomness in this example",
+    )
+    parser.add_argument(
+        "--save-model",
+        default=None,
+        type=str,
+        help="path to save model for prediction",
+    )
+    args = parser.parse_args()
+
+    return args
+
+def set_random_seed(seed):
+    # Set seed for PyTorch
+    torch.manual_seed(seed)
+    
+    # If using CUDA, set the seed for CUDA
+    if torch.cuda.is_available():
+        torch.cuda.manual_seed(seed)
+        torch.cuda.manual_seed_all(seed)  # For multi-GPU setups.
+    
+    # Set seed for NumPy
+    np.random.seed(seed)
+    
+    # Set seed for Python's random module
+    random.seed(seed)
+
+    # Set seed for Pyro
+    pyro.set_rng_seed(seed)
+
+
+def main():
+    args = parse_args()
+
+    assert (
+        (args.data_file is not None) and (
+            os.path.exists(args.data_file))
+    ), "data file must be provided"
+
+    if args.float64:
+        dtype = torch.float64
+        torch.set_default_dtype(torch.float64)
+    else:
+        dtype = torch.float32
+        torch.set_default_dtype(torch.float32)
+
+    xs = dt.fread(file=args.data_file, header=True).to_numpy()
+    us = None 
+    if args.undesired_factor_file is not None:
+        us = dt.fread(file=args.undesired_factor_file, header=True).to_numpy()
+
+    input_size = xs.shape[1]
+    undesired_size = 0 if us is None else us.shape[1]
+
+    z_dist = args.z_dist
+    d_dist = args.d_dist
+
+    # batch_size: number of cells (and labels) to be considered in a batch
+    hmap = HMAP(
+        input_size=input_size,
+        undesired_size=undesired_size,
+        codebook_size=args.codebook_size,
+        d_dim=args.d_dim,
+        d_dist=d_dist,
+        z_dim=args.z_dim,
+        z_dist=z_dist,
+        hidden_layers=args.hidden_layers,
+        hidden_layer_activation=args.hidden_layer_activation,
+        loss_func=args.likelihood,
+        inverse_dispersion=args.inverse_dispersion,
+        nn_dropout=args.layer_dropout_rate,
+        use_cuda=args.cuda,
+        config_enum=args.enum_discrete,
+        post_layer_fct=args.post_layer_function,
+        post_act_fct=args.post_activation_function,
+        dtype=dtype,
+        seed=args.seed,
+    )
+
+    hmap.fit(xs, us = us, 
+             num_epochs=args.num_epochs,
+             learning_rate=args.learning_rate,
+             batch_size=args.batch_size,
+             beta_1=args.beta_1,
+             decay_rate=args.decay_rate,
+             use_jax=args.jit,
+             config_enum=args.enum_discrete,
+             )
+
+    if args.save_model is not None:
+        HMAP.save_model(hmap, args.save_model)
+
+
+if __name__ == "__main__":
+    main()