AutoMorphoTrack 2.0.1__tar.gz → 2.1.0__tar.gz

{automorphotrack-2.0.1 → automorphotrack-2.1.0}/MANIFEST.in

@@ -1,3 +1,4 @@
 include README.md
 include LICENSE.md
+include pyproject.toml
 recursive-include automorphotrack *.py

automorphotrack-2.1.0/PKG-INFO

@@ -0,0 +1,258 @@
+Metadata-Version: 2.4
+Name: automorphotrack
+Version: 2.1.0
+Summary: Automated pipeline for mitochondrial and lysosomal detection, tracking, morphology, and colocalization analysis in microscopy images.
+Home-page: https://github.com/abayatibrain/AutoMorphoTrack
+Author: Armin Bayati, Ph.D.
+Author-email: "Armin Bayati, Ph.D." <a.bayati.brain@gmail.com>
+License: MIT
+Project-URL: Documentation, https://github.com/abayatibrain/AutoMorphoTrack
+Project-URL: Source, https://github.com/abayatibrain/AutoMorphoTrack
+Project-URL: Tracker, https://github.com/abayatibrain/AutoMorphoTrack/issues
+Keywords: image-processing,microscopy,organelle-tracking,mitochondria,lysosomes
+Classifier: Programming Language :: Python :: 3
+Classifier: Programming Language :: Python :: 3.9
+Classifier: Programming Language :: Python :: 3.10
+Classifier: Programming Language :: Python :: 3.11
+Classifier: License :: OSI Approved :: MIT License
+Classifier: Operating System :: OS Independent
+Classifier: Intended Audience :: Science/Research
+Classifier: Topic :: Scientific/Engineering :: Image Processing
+Requires-Python: >=3.9
+Description-Content-Type: text/markdown
+License-File: LICENSE.md
+Requires-Dist: numpy>=1.23.0
+Requires-Dist: pandas>=1.5.0
+Requires-Dist: matplotlib>=3.6.0
+Requires-Dist: seaborn>=0.12.0
+Requires-Dist: opencv-python>=4.6.0
+Requires-Dist: scikit-image>=0.19.0
+Requires-Dist: scipy>=1.9.0
+Requires-Dist: tifffile>=2022.8.12
+Dynamic: author
+Dynamic: home-page
+Dynamic: license-file
+Dynamic: requires-python
+
+# AutoMorphoTrack
+
+**AutoMorphoTrack** is a comprehensive, modular image-analysis pipeline for automated detection, morphology classification, shape profiling, motility tracking, and colocalization analysis of mitochondria and lysosomes in multichannel fluorescence microscopy data.
+
+Developed by **Armin Bayati, Ph.D.**
+
+---
+
+## Overview
+
+AutoMorphoTrack processes time-lapse `.tif` stacks (typically two-channel: mitochondria + lysosomes) and generates **publication-ready visual and quantitative outputs** at every analysis step. The pipeline integrates seamlessly into scientific workflows and prioritizes **transparency, reproducibility, and ease of use**.
+
+---
+
+## Key Features
+
+- **Automated organelle detection** with morphological filtering
+- **Integrated tracking** of mitochondria and lysosomes across time
+- **Shape profiling** with publication-ready violin plots
+- **Motility analysis** with velocity and displacement quantification
+- **Colocalization metrics** (Manders, Pearson coefficients)
+- **Comprehensive validation tools** for segmentation and tracking accuracy
+- **Colorblind-safe visualizations** throughout
+- **High-resolution outputs** (600 DPI publication-ready figures)
+
+---
+
+## Installation
+
+### Via PyPI (Recommended)
+```bash
+pip install automorphotrack
+```
+
+### From Source
+```bash
+git clone https://github.com/abayatibrain/AutoMorphoTrack.git
+cd AutoMorphoTrack
+pip install -e .
+```
+
+### Development Install
+```bash
+git clone https://github.com/abayatibrain/AutoMorphoTrack.git
+cd AutoMorphoTrack
+pip install -e ".[dev]"
+```
+
+---
+
+## Quick Start
+
+```python
+from automorphotrack import (
+    detect_organelles,
+    count_lysosomes_per_frame,
+    classify_morphology,
+    analyze_shape_features,
+    profile_shape_data,
+    track_organelles,
+    track_overlay,
+    analyze_motility,
+    analyze_colocalization,
+    summarize_integrated_data
+)
+
+tif_path = "path/to/Composite.tif"
+
+# Run full pipeline
+detect_organelles(tif_path)
+count_lysosomes_per_frame(tif_path)
+classify_morphology(tif_path)
+analyze_shape_features(tif_path)
+profile_shape_data()
+track_organelles(tif_path)
+track_overlay(tif_path)
+analyze_motility()
+analyze_colocalization(tif_path)
+summarize_integrated_data()
+```
+
+---
+
+## Module Overview
+
+| Module | Function | Inputs | Outputs | Purpose |
+|--------|----------|--------|---------|---------|
+| **detection** | `detect_organelles()` | TIF file | PNG, MP4, masks | Segment mitochondria and lysosomes |
+| **lyso_count** | `count_lysosomes_per_frame()` | TIF file | PNG, CSV, MP4 | Quantify lysosomes per frame |
+| **morphology** | `classify_morphology()` | TIF file | PNG, CSV, MP4 | Classify mitochondria as elongated vs. punctate |
+| **shape_features** | `analyze_shape_features()` | TIF file | PNG, CSV | Extract circularity, solidity, aspect ratio |
+| **shape_profiling** | `profile_shape_data()` | CSVs | PNG, CSV | Generate combined violin plots |
+| **tracking** | `track_organelles()` | TIF file | PNG, CSV, MP4 | Track organelle trajectories |
+| **tracking_overlay** | `track_overlay()` | TIF file | PNG, CSV, MP4 | Overlay tracks on intensity images |
+| **motility** | `analyze_motility()` | CSVs | PNG, CSV | Compute velocity and displacement |
+| **colocalization** | `analyze_colocalization()` | TIF file | PNG, CSV, MP4 | Quantify mitochondrial-lysosomal overlap |
+| **summary** | `summarize_integrated_data()` | CSVs | PNG, CSV | Correlation matrix across all metrics |
+| **validation** | `validate_segmentation()` | Masks | dict | Compute Dice, IoU, F1, precision, recall |
+| **validation** | `validate_tracking()` | CSVs | dict | Track accuracy metrics |
+| **validation** | `sensitivity_analysis()` | TIF + params | CSV, plots | Parameter sweep analysis |
+| **validation** | `generate_validation_report()` | Results | PNG, CSV | Comprehensive validation summary |
+
+---
+
+## Output Files
+
+| Step | Output Type | Example Files |
+|------|--------------|---------------|
+| **Detection** | PNG + MP4 | `Mito_Frame0.png`, `Mitochondria_Detection.mp4` |
+| **Lysosome Count** | PNG + CSV + MP4 | `Lyso_Count_Plot.png`, `Lysosome_Counts.csv` |
+| **Morphology** | PNG + MP4 + CSV | `Morphology_Frame0_Labeled.png`, `Morphology_Labeled.mp4` |
+| **Shape Features** | PNG + CSV | `Shape_Distributions.png`, `Mito_ShapeMetrics.csv` |
+| **Shape Profiling** | PNG + CSV | `Shape_ViolinPlots.png`, `Combined_ShapeData.csv` |
+| **Tracking** | PNG + MP4 + CSV | `Cumulative_Mito.png`, `Mito_Tracks.csv` |
+| **Tracking Overlay** | PNG + MP4 | `Cumulative_Composite.png`, `Composite_CumulativeTracks.mp4` |
+| **Motility** | PNG + CSV | `Motility_Distributions.png`, `Motility_Scatter.png` |
+| **Colocalization** | PNG + MP4 + CSV | `Colocalization_Frame0.png`, `Colocalization.csv` |
+| **Summary** | PNG + CSV | `Integrated_CorrelationMatrix.png`, `Integrated_Merged_Data.csv` |
+| **Validation** | PNG + CSV | `Validation_Report.png`, `Validation_Summary.csv` |
+
+---
+
+## Dependencies
+
+**Core Requirements:**
+- Python ≥ 3.9
+- numpy ≥ 1.23.0
+- pandas ≥ 1.5.0
+- matplotlib ≥ 3.6.0
+- seaborn ≥ 0.12.0
+- opencv-python ≥ 4.6.0
+- scikit-image ≥ 0.19.0
+- scipy ≥ 1.9.0
+- tifffile ≥ 2022.8.12
+
+**Optional:**
+- scikit-learn (for validation metrics)
+
+---
+
+## Visualization Design
+
+**Colorblind-Safe Palette:**
+- Mitochondria: `#0173B2` (blue)
+- Lysosomes: `#DE8F05` (orange)
+
+All visualizations use colorblind-safe colors to ensure accessibility and publication readiness.
+
+---
+
+## Statistical Notes
+
+**Correlation Analysis (Summary Module):**
+The integrated summary module uses **Spearman rank correlation** to compute relationships between extracted metrics, providing a distribution-free assessment of feature associations.
+
+---
+
+## Comparison with Existing Tools
+
+| Feature | AutoMorphoTrack | CellProfiler | MiNA | MitoGraph | Imaris |
+|---------|-----------------|--------------|------|-----------|--------|
+| **Ease of Use** | High (Python API) | Medium (GUI-heavy) | Medium | Medium | Low (proprietary) |
+| **Transparency** | Full (open source) | Partial | Partial | Partial | Closed |
+| **Lightweight** | Yes | No | No | No | No |
+| **Publication-Ready Outputs** | Yes | Yes | Partial | Partial | Yes |
+| **Tracking** | Yes | Optional | Yes | Yes | Yes |
+| **Shape Profiling** | Yes | Limited | Yes | Yes | Yes |
+| **Colocalization** | Yes | Yes | Limited | Limited | Yes |
+| **Customizable** | Yes (modular) | Yes | Limited | Limited | No |
+| **Cost** | Free | Free | Free | Free | Expensive |
+
+**What Makes AutoMorphoTrack Different:**
+- **Integrated workflow**: All analyses in one package without external dependencies
+- **Full transparency**: Complete source code and methodology documentation
+- **Lightweight**: Minimal dependencies, fast execution
+- **Publication-ready**: High-DPI outputs and colorblind-safe visualizations by default
+- **Modular design**: Use only the modules you need
+
+---
+
+## AI Assistance Note
+
+This package was developed with support from AI language models (e.g., ChatGPT, Claude). The term "AI assistance" refers to using external LLMs to help generate, review, and refine analysis code—**NOT** a built-in AI interface or algorithmic component. All algorithms are explicit, interpretable, and scientifically grounded.
+
+---
+
+## License
+
+MIT License – See [LICENSE.md](LICENSE.md) for details.
+
+---
+
+## Citation
+
+If you use this pipeline in your work, please cite:
+
+```bibtex
+@article{bayati2025automorphotrack,
+  title={AutoMorphoTrack: Automated Organelle Tracking and Morphometric Profiling Toolkit},
+  author={Bayati, Armin and others},
+  year={2025}
+}
+```
+
+Or in text:
+
+> **Bayati, A. et al.** AutoMorphoTrack: Automated Organelle Tracking and Morphometric Profiling Toolkit (2025)
+
+---
+
+## Documentation
+
+For detailed documentation, examples, and tutorials, visit:
+- **GitHub**: [github.com/abayatibrain/AutoMorphoTrack](https://github.com/abayatibrain/AutoMorphoTrack)
+- **Issues & Support**: [github.com/abayatibrain/AutoMorphoTrack/issues](https://github.com/abayatibrain/AutoMorphoTrack/issues)
+
+---
+
+## Author
+
+**Armin Bayati, Ph.D.**
+Email: [a.bayati.brain@gmail.com](mailto:a.bayati.brain@gmail.com)

automorphotrack-2.1.0/README.md

@@ -0,0 +1,222 @@
+# AutoMorphoTrack
+
+**AutoMorphoTrack** is a comprehensive, modular image-analysis pipeline for automated detection, morphology classification, shape profiling, motility tracking, and colocalization analysis of mitochondria and lysosomes in multichannel fluorescence microscopy data.
+
+Developed by **Armin Bayati, Ph.D.**
+
+---
+
+## Overview
+
+AutoMorphoTrack processes time-lapse `.tif` stacks (typically two-channel: mitochondria + lysosomes) and generates **publication-ready visual and quantitative outputs** at every analysis step. The pipeline integrates seamlessly into scientific workflows and prioritizes **transparency, reproducibility, and ease of use**.
+
+---
+
+## Key Features
+
+- **Automated organelle detection** with morphological filtering
+- **Integrated tracking** of mitochondria and lysosomes across time
+- **Shape profiling** with publication-ready violin plots
+- **Motility analysis** with velocity and displacement quantification
+- **Colocalization metrics** (Manders, Pearson coefficients)
+- **Comprehensive validation tools** for segmentation and tracking accuracy
+- **Colorblind-safe visualizations** throughout
+- **High-resolution outputs** (600 DPI publication-ready figures)
+
+---
+
+## Installation
+
+### Via PyPI (Recommended)
+```bash
+pip install automorphotrack
+```
+
+### From Source
+```bash
+git clone https://github.com/abayatibrain/AutoMorphoTrack.git
+cd AutoMorphoTrack
+pip install -e .
+```
+
+### Development Install
+```bash
+git clone https://github.com/abayatibrain/AutoMorphoTrack.git
+cd AutoMorphoTrack
+pip install -e ".[dev]"
+```
+
+---
+
+## Quick Start
+
+```python
+from automorphotrack import (
+    detect_organelles,
+    count_lysosomes_per_frame,
+    classify_morphology,
+    analyze_shape_features,
+    profile_shape_data,
+    track_organelles,
+    track_overlay,
+    analyze_motility,
+    analyze_colocalization,
+    summarize_integrated_data
+)
+
+tif_path = "path/to/Composite.tif"
+
+# Run full pipeline
+detect_organelles(tif_path)
+count_lysosomes_per_frame(tif_path)
+classify_morphology(tif_path)
+analyze_shape_features(tif_path)
+profile_shape_data()
+track_organelles(tif_path)
+track_overlay(tif_path)
+analyze_motility()
+analyze_colocalization(tif_path)
+summarize_integrated_data()
+```
+
+---
+
+## Module Overview
+
+| Module | Function | Inputs | Outputs | Purpose |
+|--------|----------|--------|---------|---------|
+| **detection** | `detect_organelles()` | TIF file | PNG, MP4, masks | Segment mitochondria and lysosomes |
+| **lyso_count** | `count_lysosomes_per_frame()` | TIF file | PNG, CSV, MP4 | Quantify lysosomes per frame |
+| **morphology** | `classify_morphology()` | TIF file | PNG, CSV, MP4 | Classify mitochondria as elongated vs. punctate |
+| **shape_features** | `analyze_shape_features()` | TIF file | PNG, CSV | Extract circularity, solidity, aspect ratio |
+| **shape_profiling** | `profile_shape_data()` | CSVs | PNG, CSV | Generate combined violin plots |
+| **tracking** | `track_organelles()` | TIF file | PNG, CSV, MP4 | Track organelle trajectories |
+| **tracking_overlay** | `track_overlay()` | TIF file | PNG, CSV, MP4 | Overlay tracks on intensity images |
+| **motility** | `analyze_motility()` | CSVs | PNG, CSV | Compute velocity and displacement |
+| **colocalization** | `analyze_colocalization()` | TIF file | PNG, CSV, MP4 | Quantify mitochondrial-lysosomal overlap |
+| **summary** | `summarize_integrated_data()` | CSVs | PNG, CSV | Correlation matrix across all metrics |
+| **validation** | `validate_segmentation()` | Masks | dict | Compute Dice, IoU, F1, precision, recall |
+| **validation** | `validate_tracking()` | CSVs | dict | Track accuracy metrics |
+| **validation** | `sensitivity_analysis()` | TIF + params | CSV, plots | Parameter sweep analysis |
+| **validation** | `generate_validation_report()` | Results | PNG, CSV | Comprehensive validation summary |
+
+---
+
+## Output Files
+
+| Step | Output Type | Example Files |
+|------|--------------|---------------|
+| **Detection** | PNG + MP4 | `Mito_Frame0.png`, `Mitochondria_Detection.mp4` |
+| **Lysosome Count** | PNG + CSV + MP4 | `Lyso_Count_Plot.png`, `Lysosome_Counts.csv` |
+| **Morphology** | PNG + MP4 + CSV | `Morphology_Frame0_Labeled.png`, `Morphology_Labeled.mp4` |
+| **Shape Features** | PNG + CSV | `Shape_Distributions.png`, `Mito_ShapeMetrics.csv` |
+| **Shape Profiling** | PNG + CSV | `Shape_ViolinPlots.png`, `Combined_ShapeData.csv` |
+| **Tracking** | PNG + MP4 + CSV | `Cumulative_Mito.png`, `Mito_Tracks.csv` |
+| **Tracking Overlay** | PNG + MP4 | `Cumulative_Composite.png`, `Composite_CumulativeTracks.mp4` |
+| **Motility** | PNG + CSV | `Motility_Distributions.png`, `Motility_Scatter.png` |
+| **Colocalization** | PNG + MP4 + CSV | `Colocalization_Frame0.png`, `Colocalization.csv` |
+| **Summary** | PNG + CSV | `Integrated_CorrelationMatrix.png`, `Integrated_Merged_Data.csv` |
+| **Validation** | PNG + CSV | `Validation_Report.png`, `Validation_Summary.csv` |
+
+---
+
+## Dependencies
+
+**Core Requirements:**
+- Python ≥ 3.9
+- numpy ≥ 1.23.0
+- pandas ≥ 1.5.0
+- matplotlib ≥ 3.6.0
+- seaborn ≥ 0.12.0
+- opencv-python ≥ 4.6.0
+- scikit-image ≥ 0.19.0
+- scipy ≥ 1.9.0
+- tifffile ≥ 2022.8.12
+
+**Optional:**
+- scikit-learn (for validation metrics)
+
+---
+
+## Visualization Design
+
+**Colorblind-Safe Palette:**
+- Mitochondria: `#0173B2` (blue)
+- Lysosomes: `#DE8F05` (orange)
+
+All visualizations use colorblind-safe colors to ensure accessibility and publication readiness.
+
+---
+
+## Statistical Notes
+
+**Correlation Analysis (Summary Module):**
+The integrated summary module uses **Spearman rank correlation** to compute relationships between extracted metrics, providing a distribution-free assessment of feature associations.
+
+---
+
+## Comparison with Existing Tools
+
+| Feature | AutoMorphoTrack | CellProfiler | MiNA | MitoGraph | Imaris |
+|---------|-----------------|--------------|------|-----------|--------|
+| **Ease of Use** | High (Python API) | Medium (GUI-heavy) | Medium | Medium | Low (proprietary) |
+| **Transparency** | Full (open source) | Partial | Partial | Partial | Closed |
+| **Lightweight** | Yes | No | No | No | No |
+| **Publication-Ready Outputs** | Yes | Yes | Partial | Partial | Yes |
+| **Tracking** | Yes | Optional | Yes | Yes | Yes |
+| **Shape Profiling** | Yes | Limited | Yes | Yes | Yes |
+| **Colocalization** | Yes | Yes | Limited | Limited | Yes |
+| **Customizable** | Yes (modular) | Yes | Limited | Limited | No |
+| **Cost** | Free | Free | Free | Free | Expensive |
+
+**What Makes AutoMorphoTrack Different:**
+- **Integrated workflow**: All analyses in one package without external dependencies
+- **Full transparency**: Complete source code and methodology documentation
+- **Lightweight**: Minimal dependencies, fast execution
+- **Publication-ready**: High-DPI outputs and colorblind-safe visualizations by default
+- **Modular design**: Use only the modules you need
+
+---
+
+## AI Assistance Note
+
+This package was developed with support from AI language models (e.g., ChatGPT, Claude). The term "AI assistance" refers to using external LLMs to help generate, review, and refine analysis code—**NOT** a built-in AI interface or algorithmic component. All algorithms are explicit, interpretable, and scientifically grounded.
+
+---
+
+## License
+
+MIT License – See [LICENSE.md](LICENSE.md) for details.
+
+---
+
+## Citation
+
+If you use this pipeline in your work, please cite:
+
+```bibtex
+@article{bayati2025automorphotrack,
+  title={AutoMorphoTrack: Automated Organelle Tracking and Morphometric Profiling Toolkit},
+  author={Bayati, Armin and others},
+  year={2025}
+}
+```
+
+Or in text:
+
+> **Bayati, A. et al.** AutoMorphoTrack: Automated Organelle Tracking and Morphometric Profiling Toolkit (2025)
+
+---
+
+## Documentation
+
+For detailed documentation, examples, and tutorials, visit:
+- **GitHub**: [github.com/abayatibrain/AutoMorphoTrack](https://github.com/abayatibrain/AutoMorphoTrack)
+- **Issues & Support**: [github.com/abayatibrain/AutoMorphoTrack/issues](https://github.com/abayatibrain/AutoMorphoTrack/issues)
+
+---
+
+## Author
+
+**Armin Bayati, Ph.D.**
+Email: [a.bayati.brain@gmail.com](mailto:a.bayati.brain@gmail.com)

{automorphotrack-2.0.1 → automorphotrack-2.1.0}/automorphotrack/__init__.py

@@ -33,7 +33,7 @@
 #     )
 # ============================================================
 
-__version__ = "2.0.1"
+__version__ = "2.1.0"
 __author__ = "Armin Bayati"
 
 # --- Utility Imports ---
@@ -55,6 +55,17 @@ from automorphotrack.tracking_overlay import track_overlay
 from automorphotrack.motility import analyze_motility
 from automorphotrack.colocalization import analyze_colocalization
 from automorphotrack.summary import summarize_integrated_data
+from automorphotrack.temporal_dynamics import analyze_temporal_dynamics
+from automorphotrack.spatial_statistics import analyze_spatial_statistics
+from automorphotrack.network_analysis import analyze_network_topology
+
+# --- Validation Module Imports ---
+from automorphotrack.validation import (
+    validate_segmentation,
+    sensitivity_analysis,
+    validate_tracking,
+    generate_validation_report
+)
 
 # --- Convenience Alias (optional shortcut API) ---
 __all__ = [
@@ -71,5 +82,12 @@ __all__ = [
     "track_overlay",
     "analyze_motility",
     "analyze_colocalization",
-    "summarize_integrated_data"
+    "summarize_integrated_data",
+    "analyze_temporal_dynamics",
+    "analyze_spatial_statistics",
+    "analyze_network_topology",
+    "validate_segmentation",
+    "sensitivity_analysis",
+    "validate_tracking",
+    "generate_validation_report"
 ]

{automorphotrack-2.0.1 → automorphotrack-2.1.0}/automorphotrack/colocalization.py

@@ -17,7 +17,7 @@ def analyze_colocalization(
     lyso_channel=1,
     out_dir="Colocalization_Outputs",
     fps=5,
-    upscale=2.0,
+    upscale=4.0,
     overlay_alpha=0.6):
 
     ensure_dir(out_dir)
@@ -77,15 +77,22 @@ def analyze_colocalization(
         mito_sum.append(mito.sum())
         lyso_sum.append(lyso.sum())
 
-        # Visualization with bright-blue overlay
-        mito_norm = (mito / mito.max() * 255).astype(np.uint8)
-        lyso_norm = (lyso / lyso.max() * 255).astype(np.uint8)
+        # Visualization with CB-safe blue (mito) / orange (lyso) overlay
+        mito_norm = (mito / (mito.max() + 1e-12) * 255).astype(np.uint8)
+        lyso_norm = (lyso / (lyso.max() + 1e-12) * 255).astype(np.uint8)
         rgb = np.zeros((*mito.shape, 3), np.uint8)
-        rgb[..., 0], rgb[..., 1] = mito_norm, lyso_norm
+        # Mito → blue (1, 115, 178)
+        rgb[..., 0] = (mito_norm * (1 / 255)).astype(np.uint8)
+        rgb[..., 1] = (mito_norm * (115 / 255)).astype(np.uint8)
+        rgb[..., 2] = (mito_norm * (178 / 255)).astype(np.uint8)
+        # Lyso → orange (222, 143, 5) added on top
+        rgb[..., 0] = np.clip(rgb[..., 0].astype(int) + (lyso_norm * (222 / 255)).astype(int), 0, 255).astype(np.uint8)
+        rgb[..., 1] = np.clip(rgb[..., 1].astype(int) + (lyso_norm * (143 / 255)).astype(int), 0, 255).astype(np.uint8)
+        rgb[..., 2] = np.clip(rgb[..., 2].astype(int) + (lyso_norm * (5 / 255)).astype(int), 0, 255).astype(np.uint8)
 
         mask = overlap_mask.astype(bool)
-        bright_blue_overlay = np.full_like(rgb[mask], (0, 150, 255), dtype=np.uint8)
-        rgb[mask] = cv2.addWeighted(rgb[mask], 1 - overlay_alpha, bright_blue_overlay, overlay_alpha, 0)
+        white_overlay = np.full_like(rgb[mask], (255, 255, 255), dtype=np.uint8)
+        rgb[mask] = cv2.addWeighted(rgb[mask], 1 - overlay_alpha, white_overlay, overlay_alpha, 0)
 
         frames_out.append(upscale_frame(rgb))
 

{automorphotrack-2.0.1 → automorphotrack-2.1.0}/automorphotrack/detection.py

@@ -23,7 +23,7 @@ def detect_organelles(
     out_dir="Detection_Outputs",
     mito_channel=0,
     lyso_channel=1,
-    upscale_factor=2,
+    upscale_factor=4,
     fps=5):
 
     ensure_dir(out_dir)

{automorphotrack-2.0.1 → automorphotrack-2.1.0}/automorphotrack/lyso_count.py

@@ -22,7 +22,7 @@ def count_lysosomes_per_frame(
     tif_path="Composite.tif",
     out_dir="Lyso_Count_Outputs",
     lyso_channel=1,
-    upscale_factor=2,
+    upscale_factor=4,
     fps=5):
 
     ensure_dir(out_dir)

{automorphotrack-2.0.1 → automorphotrack-2.1.0}/automorphotrack/morphology.py

@@ -34,7 +34,7 @@ def classify_morphology(
     ecc_thresh_elongated=0.80,
     area_thresh_punctate=0.03,
     ecc_thresh_punctate=0.60,
-    upscale_factor=2,
+    upscale_factor=4,
     fps=5):
 
     ensure_dir(out_dir)