AutoMorphoTrack 2.0.0__tar.gz → 2.0.2__tar.gz
This diff represents the content of publicly available package versions that have been released to one of the supported registries. The information contained in this diff is provided for informational purposes only and reflects changes between package versions as they appear in their respective public registries.
- automorphotrack-2.0.2/LICENSE.md +21 -0
- automorphotrack-2.0.2/PKG-INFO +133 -0
- automorphotrack-2.0.2/README.md +93 -0
- {automorphotrack-2.0.0 → automorphotrack-2.0.2}/automorphotrack/__init__.py +1 -1
- {automorphotrack-2.0.0 → automorphotrack-2.0.2}/automorphotrack/colocalization.py +13 -6
- {automorphotrack-2.0.0 → automorphotrack-2.0.2}/automorphotrack/detection.py +1 -1
- {automorphotrack-2.0.0 → automorphotrack-2.0.2}/automorphotrack/lyso_count.py +1 -1
- {automorphotrack-2.0.0 → automorphotrack-2.0.2}/automorphotrack/morphology.py +1 -1
- {automorphotrack-2.0.0 → automorphotrack-2.0.2}/automorphotrack/shape_features.py +1 -1
- automorphotrack-2.0.2/automorphotrack.egg-info/PKG-INFO +133 -0
- {automorphotrack-2.0.0 → automorphotrack-2.0.2}/automorphotrack.egg-info/SOURCES.txt +2 -0
- {automorphotrack-2.0.0 → automorphotrack-2.0.2}/setup.py +1 -1
- automorphotrack-2.0.0/PKG-INFO +0 -36
- automorphotrack-2.0.0/automorphotrack.egg-info/PKG-INFO +0 -36
- {automorphotrack-2.0.0 → automorphotrack-2.0.2}/MANIFEST.in +0 -0
- {automorphotrack-2.0.0 → automorphotrack-2.0.2}/automorphotrack/.___init__.py +0 -0
- {automorphotrack-2.0.0 → automorphotrack-2.0.2}/automorphotrack/._colocalization.py +0 -0
- {automorphotrack-2.0.0 → automorphotrack-2.0.2}/automorphotrack/._detection.py +0 -0
- {automorphotrack-2.0.0 → automorphotrack-2.0.2}/automorphotrack/._lyso_count.py +0 -0
- {automorphotrack-2.0.0 → automorphotrack-2.0.2}/automorphotrack/._morphology.py +0 -0
- {automorphotrack-2.0.0 → automorphotrack-2.0.2}/automorphotrack/._motility.py +0 -0
- {automorphotrack-2.0.0 → automorphotrack-2.0.2}/automorphotrack/._shape_features.py +0 -0
- {automorphotrack-2.0.0 → automorphotrack-2.0.2}/automorphotrack/._shape_profiling.py +0 -0
- {automorphotrack-2.0.0 → automorphotrack-2.0.2}/automorphotrack/._summary.py +0 -0
- {automorphotrack-2.0.0 → automorphotrack-2.0.2}/automorphotrack/._tracking.py +0 -0
- {automorphotrack-2.0.0 → automorphotrack-2.0.2}/automorphotrack/._tracking_overlay.py +0 -0
- {automorphotrack-2.0.0 → automorphotrack-2.0.2}/automorphotrack/._utils.py +0 -0
- {automorphotrack-2.0.0 → automorphotrack-2.0.2}/automorphotrack/motility.py +0 -0
- {automorphotrack-2.0.0 → automorphotrack-2.0.2}/automorphotrack/shape_profiling.py +0 -0
- {automorphotrack-2.0.0 → automorphotrack-2.0.2}/automorphotrack/summary.py +0 -0
- {automorphotrack-2.0.0 → automorphotrack-2.0.2}/automorphotrack/tracking.py +0 -0
- {automorphotrack-2.0.0 → automorphotrack-2.0.2}/automorphotrack/tracking_overlay.py +0 -0
- {automorphotrack-2.0.0 → automorphotrack-2.0.2}/automorphotrack/utils.py +0 -0
- {automorphotrack-2.0.0 → automorphotrack-2.0.2}/automorphotrack.egg-info/dependency_links.txt +0 -0
- {automorphotrack-2.0.0 → automorphotrack-2.0.2}/automorphotrack.egg-info/requires.txt +0 -0
- {automorphotrack-2.0.0 → automorphotrack-2.0.2}/automorphotrack.egg-info/top_level.txt +0 -0
- {automorphotrack-2.0.0 → automorphotrack-2.0.2}/pyproject.toml +0 -0
- {automorphotrack-2.0.0 → automorphotrack-2.0.2}/setup.cfg +0 -0
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MIT License
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Copyright (c) 2025 Armin Bayati
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Permission is hereby granted, free of charge, to any person obtaining a copy
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of this software and associated documentation files (the "Software"), to deal
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in the Software without restriction, including without limitation the rights
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to use, copy, modify, merge, publish, distribute, sublicense, and/or sell
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copies of the Software, and to permit persons to whom the Software is
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furnished to do so, subject to the following conditions:
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The above copyright notice and this permission notice shall be included in all
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copies or substantial portions of the Software.
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THE SOFTWARE IS PROVIDED "AS IS", WITHOUT WARRANTY OF ANY KIND, EXPRESS OR
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IMPLIED, INCLUDING BUT NOT LIMITED TO THE WARRANTIES OF MERCHANTABILITY,
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FITNESS FOR A PARTICULAR PURPOSE AND NONINFRINGEMENT. IN NO EVENT SHALL THE
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AUTHORS OR COPYRIGHT HOLDERS BE LIABLE FOR ANY CLAIM, DAMAGES OR OTHER
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LIABILITY, WHETHER IN AN ACTION OF CONTRACT, TORT OR OTHERWISE, ARISING FROM,
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OUT OF OR IN CONNECTION WITH THE SOFTWARE OR THE USE OR OTHER DEALINGS IN THE
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SOFTWARE.
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Metadata-Version: 2.4
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Name: automorphotrack
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Version: 2.0.2
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Summary: Automated pipeline for mitochondrial and lysosomal detection, tracking, morphology, and colocalization analysis in microscopy images.
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Home-page: https://github.com/abayatibrain/AMTpackage
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Author: Armin Bayati, Ph.D.
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Author-email: a.bayati.brain@gmail.com
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License: MIT
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Project-URL: Documentation, https://github.com/abayatibrain/AMTpackage
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Project-URL: Source, https://github.com/abayatibrain/AMTpackage
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Project-URL: Tracker, https://github.com/abayatibrain/AMTpackage/issues
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Classifier: Programming Language :: Python :: 3
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Classifier: License :: OSI Approved :: MIT License
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Classifier: Operating System :: OS Independent
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Classifier: Intended Audience :: Science/Research
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Classifier: Topic :: Scientific/Engineering :: Image Processing
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Requires-Python: >=3.9
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Description-Content-Type: text/markdown
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License-File: LICENSE.md
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Requires-Dist: numpy>=1.23.0
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Requires-Dist: pandas>=1.5.0
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Requires-Dist: matplotlib>=3.6.0
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Requires-Dist: seaborn>=0.12.0
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Requires-Dist: opencv-python>=4.6.0
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Requires-Dist: scikit-image>=0.19.0
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Requires-Dist: scipy>=1.9.0
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Requires-Dist: tifffile>=2022.8.12
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Dynamic: author
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Dynamic: author-email
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Dynamic: classifier
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Dynamic: description
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Dynamic: requires-dist
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Dynamic: requires-python
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Dynamic: summary
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# AutoMorphoTrack
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**AutoMorphoTrack** is a modular image-analysis pipeline for automated detection, morphology classification, shape profiling, motility tracking, and colocalization analysis of mitochondria and lysosomes in multichannel fluorescence microscopy data.
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Developed by **Armin Bayati, Ph.D.**
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---
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## 🧬 Overview
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AutoMorphoTrack processes time-lapse `.tif` stacks (typically two-channel: mitochondria + lysosomes) and generates publication-ready visual and quantitative outputs at every step.
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**Pipeline stages:**
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1. **Detection** – Organelle segmentation and outline visualization
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2. **Lysosomal Counting** – Per-frame lysosome counts and plots
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3. **Morphology Classification** – Elongated vs. punctate mitochondria
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4. **Shape Feature Extraction** – Circularity, solidity, aspect ratio, orientation
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5. **Shape Profiling** – Combined violin plots of mitochondrial and lysosomal metrics
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6. **Tracking** – Cumulative organelle trajectories (mitochondria, lysosomes, composite)
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7. **Tracking Overlay** – Tracks drawn on real-intensity images
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8. **Motility Analysis** – Velocity and displacement distributions + scatter plots
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9. **Colocalization** – Bright-blue overlap visualization with Manders and Pearson coefficients
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10. **Integrated Summary** – Correlation matrix across all extracted metrics
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---
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## 📁 Installation
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```bash
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pip install automorphotrack
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```
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Or clone directly:
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```bash
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git clone https://github.com/abayatibrain/AutoMorphoTrack.git
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cd AutoMorphoTrack
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pip install -e .
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```
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---
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## 🚀 Basic Usage
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```python
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from automorphotrack import *
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tif_path = "Composite.tif"
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detect_organelles(tif_path)
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count_lysosomes_per_frame(tif_path)
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classify_morphology(tif_path)
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analyze_shape_features(tif_path)
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profile_shape_data()
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track_organelles(tif_path)
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track_overlay(tif_path)
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analyze_motility()
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analyze_colocalization(tif_path)
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summarize_integrated_data()
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```
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---
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## 📦 Outputs
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| Step | Output Type | Example Files |
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|------|--------------|---------------|
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| Detection | PNG + MP4 | `Mito_Frame0.png`, `Mitochondria_Detection.mp4` |
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| Lysosome Count | PNG + CSV + MP4 | `Lyso_Count_Plot.png`, `Lysosome_Counts.csv` |
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| Morphology | PNG + MP4 + CSV | `Morphology_Frame0_Labeled.png`, `Morphology_Labeled.mp4` |
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| Shape Features | PNG + CSV | `Shape_Distributions.png`, `Mito_ShapeMetrics.csv` |
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| Shape Profiling | PNG + CSV | `Shape_ViolinPlots.png`, `Combined_ShapeData.csv` |
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| Tracking | PNG + MP4 + CSV | `Cumulative_Mito.png`, `Mito_Tracks.csv` |
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| Tracking Overlay | PNG + MP4 | `Cumulative_Composite.png`, `Composite_CumulativeTracks.mp4` |
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| Motility | PNG + CSV | `Motility_Distributions.png`, `Motility_Scatter.png` |
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| Colocalization | PNG + MP4 + CSV | `Colocalization_Frame0.png`, `Colocalization.csv` |
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| Summary | PNG + CSV | `Integrated_CorrelationMatrix.png`, `Integrated_Merged_Data.csv` |
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---
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## 🔧 Dependencies
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- Python ≥ 3.9
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- numpy, pandas, matplotlib, seaborn, opencv-python, scikit-image, scipy, tifffile
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---
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## 🧩 Citation
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If you use this pipeline in your work, please cite:
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> **Bayati, A. et al.** AutoMorphoTrack: Automated Organelle Tracking and Morphometric Profiling Toolkit (2025)
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# AutoMorphoTrack
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**AutoMorphoTrack** is a modular image-analysis pipeline for automated detection, morphology classification, shape profiling, motility tracking, and colocalization analysis of mitochondria and lysosomes in multichannel fluorescence microscopy data.
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Developed by **Armin Bayati, Ph.D.**
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---
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## 🧬 Overview
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AutoMorphoTrack processes time-lapse `.tif` stacks (typically two-channel: mitochondria + lysosomes) and generates publication-ready visual and quantitative outputs at every step.
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**Pipeline stages:**
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1. **Detection** – Organelle segmentation and outline visualization
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2. **Lysosomal Counting** – Per-frame lysosome counts and plots
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3. **Morphology Classification** – Elongated vs. punctate mitochondria
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4. **Shape Feature Extraction** – Circularity, solidity, aspect ratio, orientation
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5. **Shape Profiling** – Combined violin plots of mitochondrial and lysosomal metrics
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6. **Tracking** – Cumulative organelle trajectories (mitochondria, lysosomes, composite)
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7. **Tracking Overlay** – Tracks drawn on real-intensity images
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8. **Motility Analysis** – Velocity and displacement distributions + scatter plots
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9. **Colocalization** – Bright-blue overlap visualization with Manders and Pearson coefficients
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10. **Integrated Summary** – Correlation matrix across all extracted metrics
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---
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## 📁 Installation
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```bash
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pip install automorphotrack
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```
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Or clone directly:
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```bash
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git clone https://github.com/abayatibrain/AutoMorphoTrack.git
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cd AutoMorphoTrack
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pip install -e .
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```
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---
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## 🚀 Basic Usage
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```python
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from automorphotrack import *
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tif_path = "Composite.tif"
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detect_organelles(tif_path)
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count_lysosomes_per_frame(tif_path)
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classify_morphology(tif_path)
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analyze_shape_features(tif_path)
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profile_shape_data()
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track_organelles(tif_path)
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track_overlay(tif_path)
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analyze_motility()
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analyze_colocalization(tif_path)
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summarize_integrated_data()
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```
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---
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## 📦 Outputs
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| Step | Output Type | Example Files |
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|------|--------------|---------------|
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| Detection | PNG + MP4 | `Mito_Frame0.png`, `Mitochondria_Detection.mp4` |
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| Lysosome Count | PNG + CSV + MP4 | `Lyso_Count_Plot.png`, `Lysosome_Counts.csv` |
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| Morphology | PNG + MP4 + CSV | `Morphology_Frame0_Labeled.png`, `Morphology_Labeled.mp4` |
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| Shape Features | PNG + CSV | `Shape_Distributions.png`, `Mito_ShapeMetrics.csv` |
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| Shape Profiling | PNG + CSV | `Shape_ViolinPlots.png`, `Combined_ShapeData.csv` |
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| Tracking | PNG + MP4 + CSV | `Cumulative_Mito.png`, `Mito_Tracks.csv` |
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| Tracking Overlay | PNG + MP4 | `Cumulative_Composite.png`, `Composite_CumulativeTracks.mp4` |
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| Motility | PNG + CSV | `Motility_Distributions.png`, `Motility_Scatter.png` |
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| Colocalization | PNG + MP4 + CSV | `Colocalization_Frame0.png`, `Colocalization.csv` |
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| Summary | PNG + CSV | `Integrated_CorrelationMatrix.png`, `Integrated_Merged_Data.csv` |
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---
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## 🔧 Dependencies
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---
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## 🧩 Citation
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If you use this pipeline in your work, please cite:
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> **Bayati, A. et al.** AutoMorphoTrack: Automated Organelle Tracking and Morphometric Profiling Toolkit (2025)
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# Visualization with
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mito_norm = (mito / mito.max() * 255).astype(np.uint8)
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# Visualization with CB-safe blue (mito) / orange (lyso) overlay
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mito_norm = (mito / (mito.max() + 1e-12) * 255).astype(np.uint8)
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lyso_norm = (lyso / (lyso.max() + 1e-12) * 255).astype(np.uint8)
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# Mito → blue (1, 115, 178)
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rgb[..., 0] = (mito_norm * (1 / 255)).astype(np.uint8)
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rgb[..., 1] = (mito_norm * (115 / 255)).astype(np.uint8)
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rgb[..., 2] = (mito_norm * (178 / 255)).astype(np.uint8)
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# Lyso → orange (222, 143, 5) added on top
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rgb[..., 1] = np.clip(rgb[..., 1].astype(int) + (lyso_norm * (143 / 255)).astype(int), 0, 255).astype(np.uint8)
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rgb[..., 2] = np.clip(rgb[..., 2].astype(int) + (lyso_norm * (5 / 255)).astype(int), 0, 255).astype(np.uint8)
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rgb[mask] = cv2.addWeighted(rgb[mask], 1 - overlay_alpha, white_overlay, overlay_alpha, 0)
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frames_out.append(upscale_frame(rgb))
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"""Detect lysosomes in a single channel frame."""
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def detect_mask(frame, ch, thr_factor=0.8, min_size=10):
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"""Threshold a channel to obtain clean mitochondrial mask."""
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def detect_mask(frame, ch, min_size=3, thr_factor=0.8):
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@@ -0,0 +1,133 @@
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Metadata-Version: 2.4
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Name: automorphotrack
|
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3
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+
Version: 2.0.2
|
|
4
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+
Summary: Automated pipeline for mitochondrial and lysosomal detection, tracking, morphology, and colocalization analysis in microscopy images.
|
|
5
|
+
Home-page: https://github.com/abayatibrain/AMTpackage
|
|
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|
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Author: Armin Bayati, Ph.D.
|
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Author-email: a.bayati.brain@gmail.com
|
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License: MIT
|
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Project-URL: Documentation, https://github.com/abayatibrain/AMTpackage
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Project-URL: Source, https://github.com/abayatibrain/AMTpackage
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Project-URL: Tracker, https://github.com/abayatibrain/AMTpackage/issues
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Classifier: Programming Language :: Python :: 3
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Classifier: License :: OSI Approved :: MIT License
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Classifier: Operating System :: OS Independent
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Classifier: Intended Audience :: Science/Research
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Classifier: Topic :: Scientific/Engineering :: Image Processing
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Requires-Python: >=3.9
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Description-Content-Type: text/markdown
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License-File: LICENSE.md
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Requires-Dist: numpy>=1.23.0
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Requires-Dist: seaborn>=0.12.0
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Requires-Dist: opencv-python>=4.6.0
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Requires-Dist: scikit-image>=0.19.0
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Requires-Dist: scipy>=1.9.0
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Requires-Dist: tifffile>=2022.8.12
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Dynamic: author
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Dynamic: author-email
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Dynamic: classifier
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Dynamic: description
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|
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# AutoMorphoTrack
|
|
42
|
+
|
|
43
|
+
**AutoMorphoTrack** is a modular image-analysis pipeline for automated detection, morphology classification, shape profiling, motility tracking, and colocalization analysis of mitochondria and lysosomes in multichannel fluorescence microscopy data.
|
|
44
|
+
|
|
45
|
+
Developed by **Armin Bayati, Ph.D.**
|
|
46
|
+
|
|
47
|
+
---
|
|
48
|
+
|
|
49
|
+
## 🧬 Overview
|
|
50
|
+
|
|
51
|
+
AutoMorphoTrack processes time-lapse `.tif` stacks (typically two-channel: mitochondria + lysosomes) and generates publication-ready visual and quantitative outputs at every step.
|
|
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|
+
|
|
53
|
+
**Pipeline stages:**
|
|
54
|
+
|
|
55
|
+
1. **Detection** – Organelle segmentation and outline visualization
|
|
56
|
+
2. **Lysosomal Counting** – Per-frame lysosome counts and plots
|
|
57
|
+
3. **Morphology Classification** – Elongated vs. punctate mitochondria
|
|
58
|
+
4. **Shape Feature Extraction** – Circularity, solidity, aspect ratio, orientation
|
|
59
|
+
5. **Shape Profiling** – Combined violin plots of mitochondrial and lysosomal metrics
|
|
60
|
+
6. **Tracking** – Cumulative organelle trajectories (mitochondria, lysosomes, composite)
|
|
61
|
+
7. **Tracking Overlay** – Tracks drawn on real-intensity images
|
|
62
|
+
8. **Motility Analysis** – Velocity and displacement distributions + scatter plots
|
|
63
|
+
9. **Colocalization** – Bright-blue overlap visualization with Manders and Pearson coefficients
|
|
64
|
+
10. **Integrated Summary** – Correlation matrix across all extracted metrics
|
|
65
|
+
|
|
66
|
+
---
|
|
67
|
+
|
|
68
|
+
## 📁 Installation
|
|
69
|
+
|
|
70
|
+
```bash
|
|
71
|
+
pip install automorphotrack
|
|
72
|
+
```
|
|
73
|
+
|
|
74
|
+
Or clone directly:
|
|
75
|
+
|
|
76
|
+
```bash
|
|
77
|
+
git clone https://github.com/abayatibrain/AutoMorphoTrack.git
|
|
78
|
+
cd AutoMorphoTrack
|
|
79
|
+
pip install -e .
|
|
80
|
+
```
|
|
81
|
+
|
|
82
|
+
---
|
|
83
|
+
|
|
84
|
+
## 🚀 Basic Usage
|
|
85
|
+
|
|
86
|
+
```python
|
|
87
|
+
from automorphotrack import *
|
|
88
|
+
|
|
89
|
+
tif_path = "Composite.tif"
|
|
90
|
+
|
|
91
|
+
detect_organelles(tif_path)
|
|
92
|
+
count_lysosomes_per_frame(tif_path)
|
|
93
|
+
classify_morphology(tif_path)
|
|
94
|
+
analyze_shape_features(tif_path)
|
|
95
|
+
profile_shape_data()
|
|
96
|
+
track_organelles(tif_path)
|
|
97
|
+
track_overlay(tif_path)
|
|
98
|
+
analyze_motility()
|
|
99
|
+
analyze_colocalization(tif_path)
|
|
100
|
+
summarize_integrated_data()
|
|
101
|
+
```
|
|
102
|
+
|
|
103
|
+
---
|
|
104
|
+
|
|
105
|
+
## 📦 Outputs
|
|
106
|
+
|
|
107
|
+
| Step | Output Type | Example Files |
|
|
108
|
+
|------|--------------|---------------|
|
|
109
|
+
| Detection | PNG + MP4 | `Mito_Frame0.png`, `Mitochondria_Detection.mp4` |
|
|
110
|
+
| Lysosome Count | PNG + CSV + MP4 | `Lyso_Count_Plot.png`, `Lysosome_Counts.csv` |
|
|
111
|
+
| Morphology | PNG + MP4 + CSV | `Morphology_Frame0_Labeled.png`, `Morphology_Labeled.mp4` |
|
|
112
|
+
| Shape Features | PNG + CSV | `Shape_Distributions.png`, `Mito_ShapeMetrics.csv` |
|
|
113
|
+
| Shape Profiling | PNG + CSV | `Shape_ViolinPlots.png`, `Combined_ShapeData.csv` |
|
|
114
|
+
| Tracking | PNG + MP4 + CSV | `Cumulative_Mito.png`, `Mito_Tracks.csv` |
|
|
115
|
+
| Tracking Overlay | PNG + MP4 | `Cumulative_Composite.png`, `Composite_CumulativeTracks.mp4` |
|
|
116
|
+
| Motility | PNG + CSV | `Motility_Distributions.png`, `Motility_Scatter.png` |
|
|
117
|
+
| Colocalization | PNG + MP4 + CSV | `Colocalization_Frame0.png`, `Colocalization.csv` |
|
|
118
|
+
| Summary | PNG + CSV | `Integrated_CorrelationMatrix.png`, `Integrated_Merged_Data.csv` |
|
|
119
|
+
|
|
120
|
+
---
|
|
121
|
+
|
|
122
|
+
## 🔧 Dependencies
|
|
123
|
+
|
|
124
|
+
- Python ≥ 3.9
|
|
125
|
+
- numpy, pandas, matplotlib, seaborn, opencv-python, scikit-image, scipy, tifffile
|
|
126
|
+
|
|
127
|
+
---
|
|
128
|
+
|
|
129
|
+
## 🧩 Citation
|
|
130
|
+
|
|
131
|
+
If you use this pipeline in your work, please cite:
|
|
132
|
+
|
|
133
|
+
> **Bayati, A. et al.** AutoMorphoTrack: Automated Organelle Tracking and Morphometric Profiling Toolkit (2025)
|
|
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|
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|
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12
|
name="automorphotrack",
|
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|
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version="2.0.
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version="2.0.2",
|
|
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14
|
author="Armin Bayati, Ph.D.",
|
|
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15
|
author_email="a.bayati.brain@gmail.com",
|
|
16
16
|
description="Automated pipeline for mitochondrial and lysosomal detection, tracking, morphology, and colocalization analysis in microscopy images.",
|
automorphotrack-2.0.0/PKG-INFO
DELETED
|
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Metadata-Version: 2.4
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Name: automorphotrack
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|
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Version: 2.0.0
|
|
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|
-
Summary: Automated pipeline for mitochondrial and lysosomal detection, tracking, morphology, and colocalization analysis in microscopy images.
|
|
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|
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Home-page: https://github.com/abayatibrain/AMTpackage
|
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Author: Armin Bayati, Ph.D.
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License: MIT
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Project-URL: Documentation, https://github.com/abayatibrain/AMTpackage
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Project-URL: Tracker, https://github.com/abayatibrain/AMTpackage/issues
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Classifier: Programming Language :: Python :: 3
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Name: automorphotrack
|
|
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Version: 2.0.0
|
|
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|
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Summary: Automated pipeline for mitochondrial and lysosomal detection, tracking, morphology, and colocalization analysis in microscopy images.
|
|
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Home-page: https://github.com/abayatibrain/AMTpackage
|
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Author: Armin Bayati, Ph.D.
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{automorphotrack-2.0.0 → automorphotrack-2.0.2}/automorphotrack.egg-info/dependency_links.txt
RENAMED
|
File without changes
|
|
File without changes
|
|
File without changes
|
|
File without changes
|
|
File without changes
|