simple_bioc 0.0.7 → 0.0.8
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- checksums.yaml +4 -4
- data/lib/simple_bioc/bioc_merger.rb +8 -2
- data/lib/simple_bioc/version.rb +1 -1
- data/spec/file_check_spec.rb +29 -0
- data/xml/merge/output.xml +61 -3201
- metadata +2 -2
checksums.yaml
CHANGED
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@@ -1,7 +1,7 @@
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1
1
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---
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2
2
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SHA1:
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3
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-
metadata.gz:
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4
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-
data.tar.gz:
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3
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+
metadata.gz: 2db1d0eca30d1829335e2953febac31c9d9278f7
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4
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+
data.tar.gz: 3ccb2c94824e37ffc5cf9576b15328d37f880a68
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5
5
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SHA512:
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6
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-
metadata.gz:
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7
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-
data.tar.gz:
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6
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+
metadata.gz: 81deae4a51d85fc659680359ba4e3dc0d8d09b006f8c5272041a8a8773b0c59b9f4f78d851dcc408ec0cd5d12197e9cde49aa98072ccbfb11965741be78fb542
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|
7
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+
data.tar.gz: c9fd968958871c146bcba7f468c04f152269daaf6b91425ee98cab129bb0a32d2445d227b4c914c88a3336feeae246106dc92f6dcefe9762482189e909aad95a
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|
@@ -31,15 +31,17 @@ module BioCMerger
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|
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31
31
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|
32
32
|
doc_d.passages.each_with_index do |p_d, index|
|
|
33
33
|
p_s = doc_s.passages[index]
|
|
34
|
-
if
|
|
34
|
+
if p_d.nil? || p_s.nil?
|
|
35
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+
warnings << 'The number of sentences in pages should be the same'
|
|
36
|
+
elsif blank?(p_d.text) && blank?(p_s.text) && p_d.sentences.size != p_s.sentences.size
|
|
35
37
|
warnings << 'The number of sentences in pages should be the same'
|
|
36
38
|
end
|
|
37
39
|
end
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|
38
40
|
|
|
39
41
|
doc_d.passages.each_with_index do |p_d, index|
|
|
40
42
|
p_s = doc_s.passages[index]
|
|
43
|
+
next if p_d.nil? || p_s.nil?
|
|
41
44
|
copy_relations(doc_d, p_d, p_s, id_map)
|
|
42
|
-
|
|
43
45
|
if p_d.sentences.size == p_s.sentences.size
|
|
44
46
|
p_d.sentences.each_with_index do |s_d, index|
|
|
45
47
|
s_s = p_s.sentences[index]
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|
@@ -81,6 +83,7 @@ module BioCMerger
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|
|
81
83
|
end
|
|
82
84
|
|
|
83
85
|
def adjust_annotation_offset(obj)
|
|
86
|
+
return if obj.nil?
|
|
84
87
|
obj.annotations.each do |a|
|
|
85
88
|
positions = find_all_locations(obj, a.text)
|
|
86
89
|
a.locations.each do |l|
|
|
@@ -100,6 +103,7 @@ module BioCMerger
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|
|
100
103
|
end
|
|
101
104
|
|
|
102
105
|
def adjust_relation_refid(obj, id_map)
|
|
106
|
+
return if obj.nil?
|
|
103
107
|
obj.relations.each do |r|
|
|
104
108
|
next if r.original.nil?
|
|
105
109
|
r.nodes.each do |n|
|
|
@@ -111,12 +115,14 @@ module BioCMerger
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|
|
111
115
|
end
|
|
112
116
|
|
|
113
117
|
def copy_relations(doc, dest, src, id_map)
|
|
118
|
+
return if src.nil?
|
|
114
119
|
src.relations.each do |r|
|
|
115
120
|
copy_relation(doc, dest, r, id_map)
|
|
116
121
|
end
|
|
117
122
|
end
|
|
118
123
|
|
|
119
124
|
def copy_annotations(doc, dest, src, id_map)
|
|
125
|
+
return if src.nil?
|
|
120
126
|
src.annotations.each do |a|
|
|
121
127
|
copy_annotation(doc, dest, a, id_map)
|
|
122
128
|
end
|
data/lib/simple_bioc/version.rb
CHANGED
data/spec/file_check_spec.rb
CHANGED
|
@@ -22,5 +22,34 @@ describe "File Check" do
|
|
|
22
22
|
SimpleBioC.merge(col1, col4)
|
|
23
23
|
output = SimpleBioC.to_xml(col1)
|
|
24
24
|
File.write("./xml/merge/output.xml", output)
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|
25
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+
puts "merge1"
|
|
26
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+
col5 = SimpleBioC.from_xml("./xml/merge/output.xml")
|
|
25
27
|
end
|
|
28
|
+
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|
29
|
+
it "should merge documents successfully with different order" do
|
|
30
|
+
col4 = SimpleBioC.from_xml("./xml/merge/9864355.xml")
|
|
31
|
+
col3 = SimpleBioC.from_xml("./xml/merge/9864355_1.xml")
|
|
32
|
+
col1 = SimpleBioC.from_xml("./xml/merge/9864355_2.xml")
|
|
33
|
+
col2 = SimpleBioC.from_xml("./xml/merge/9864355_3.xml")
|
|
34
|
+
|
|
35
|
+
SimpleBioC.merge(col1, col2)
|
|
36
|
+
SimpleBioC.merge(col1, col3)
|
|
37
|
+
SimpleBioC.merge(col1, col4)
|
|
38
|
+
output = SimpleBioC.to_xml(col1)
|
|
39
|
+
File.write("./xml/merge/output.xml", output)
|
|
40
|
+
puts "merge12"
|
|
41
|
+
col5 = SimpleBioC.from_xml("./xml/merge/output.xml")
|
|
42
|
+
end
|
|
43
|
+
|
|
44
|
+
it "should merge documents successfully with abbr" do
|
|
45
|
+
col1 = SimpleBioC.from_xml("./xml/merge/9864355.xml")
|
|
46
|
+
col2 = SimpleBioC.from_xml("./xml/abbr.xml")
|
|
47
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+
|
|
48
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+
SimpleBioC.merge(col1, col2)
|
|
49
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+
output = SimpleBioC.to_xml(col1)
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|
50
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+
File.write("./xml/merge/output.xml", output)
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|
51
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+
puts "merge12"
|
|
52
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+
col5 = SimpleBioC.from_xml("./xml/merge/output.xml")
|
|
53
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+
end
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|
54
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+
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26
55
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end
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data/xml/merge/output.xml
CHANGED
|
@@ -35,11 +35,6 @@
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35
35
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<location offset="25" length="5"/>
|
|
36
36
|
<text>Ndc1p</text>
|
|
37
37
|
</annotation>
|
|
38
|
-
<annotation id="G1">
|
|
39
|
-
<infon key="type">Gene</infon>
|
|
40
|
-
<location offset="25" length="5"/>
|
|
41
|
-
<text>Ndc1p</text>
|
|
42
|
-
</annotation>
|
|
43
38
|
</passage>
|
|
44
39
|
<passage>
|
|
45
40
|
<infon key="type">abstract</infon>
|
|
@@ -145,107 +140,6 @@
|
|
|
145
140
|
<location offset="1154" length="189"/>
|
|
146
141
|
<text>Finally, we have found that a deletion of POM152, which encodes an abundant but nonessential nucleoporin, suppresses the SPB duplication defect associated with a mutation in the NDC1 gene</text>
|
|
147
142
|
</annotation>
|
|
148
|
-
<annotation id="G2">
|
|
149
|
-
<infon key="type">Gene</infon>
|
|
150
|
-
<location offset="234" length="35"/>
|
|
151
|
-
<text>Saccharomyces cerevisiae NDC1 gene</text>
|
|
152
|
-
</annotation>
|
|
153
|
-
<annotation id="G3">
|
|
154
|
-
<infon key="score">0.70</infon>
|
|
155
|
-
<infon key="GeneID">335236</infon>
|
|
156
|
-
<infon key="type">Gene</infon>
|
|
157
|
-
<location offset="428" length="4"/>
|
|
158
|
-
<text>NDC1</text>
|
|
159
|
-
</annotation>
|
|
160
|
-
<annotation id="G4">
|
|
161
|
-
<infon key="score">0.73</infon>
|
|
162
|
-
<infon key="GeneID">854977</infon>
|
|
163
|
-
<infon key="type">Gene</infon>
|
|
164
|
-
<location offset="606" length="5"/>
|
|
165
|
-
<text>Ndc1p</text>
|
|
166
|
-
</annotation>
|
|
167
|
-
<annotation id="G5">
|
|
168
|
-
<infon key="score">0.73</infon>
|
|
169
|
-
<infon key="GeneID">854977</infon>
|
|
170
|
-
<infon key="type">Gene</infon>
|
|
171
|
-
<location offset="734" length="5"/>
|
|
172
|
-
<text>Ndc1p</text>
|
|
173
|
-
</annotation>
|
|
174
|
-
<annotation id="G6">
|
|
175
|
-
<infon key="score">0.73</infon>
|
|
176
|
-
<infon key="GeneID">852703</infon>
|
|
177
|
-
<infon key="type">Gene</infon>
|
|
178
|
-
<location offset="837" length="6"/>
|
|
179
|
-
<text>Nup49p</text>
|
|
180
|
-
</annotation>
|
|
181
|
-
<annotation id="G7">
|
|
182
|
-
<infon key="score">0.73</infon>
|
|
183
|
-
<infon key="GeneID">854977</infon>
|
|
184
|
-
<infon key="type">Gene</infon>
|
|
185
|
-
<location offset="878" length="5"/>
|
|
186
|
-
<text>Ndc1p</text>
|
|
187
|
-
</annotation>
|
|
188
|
-
<annotation id="G8">
|
|
189
|
-
<infon key="score">0.73</infon>
|
|
190
|
-
<infon key="GeneID">853824</infon>
|
|
191
|
-
<infon key="type">Gene</infon>
|
|
192
|
-
<location offset="936" length="6"/>
|
|
193
|
-
<text>Spc42p</text>
|
|
194
|
-
</annotation>
|
|
195
|
-
<annotation id="G9">
|
|
196
|
-
<infon key="score">0.73</infon>
|
|
197
|
-
<infon key="GeneID">854977</infon>
|
|
198
|
-
<infon key="type">Gene</infon>
|
|
199
|
-
<location offset="982" length="5"/>
|
|
200
|
-
<text>Ndc1p</text>
|
|
201
|
-
</annotation>
|
|
202
|
-
<annotation id="G10">
|
|
203
|
-
<infon key="score">0.70</infon>
|
|
204
|
-
<infon key="GeneID">855159</infon>
|
|
205
|
-
<infon key="type">Gene</infon>
|
|
206
|
-
<location offset="1197" length="6"/>
|
|
207
|
-
<text>POM152</text>
|
|
208
|
-
</annotation>
|
|
209
|
-
<annotation id="G11">
|
|
210
|
-
<infon key="score">0.70</infon>
|
|
211
|
-
<infon key="GeneID">335236</infon>
|
|
212
|
-
<infon key="type">Gene</infon>
|
|
213
|
-
<location offset="1334" length="9"/>
|
|
214
|
-
<text>NDC1 gene</text>
|
|
215
|
-
</annotation>
|
|
216
|
-
<annotation id="G12">
|
|
217
|
-
<infon key="score">0.73</infon>
|
|
218
|
-
<infon key="GeneID">854977</infon>
|
|
219
|
-
<infon key="type">Gene</infon>
|
|
220
|
-
<location offset="1359" length="5"/>
|
|
221
|
-
<text>Ndc1p</text>
|
|
222
|
-
</annotation>
|
|
223
|
-
<annotation id="TEAM_277_0">
|
|
224
|
-
<infon key="type">Gene</infon>
|
|
225
|
-
<location offset="260" length="4"/>
|
|
226
|
-
<text>NDC1</text>
|
|
227
|
-
</annotation>
|
|
228
|
-
<annotation id="TEAM_277_1">
|
|
229
|
-
<infon key="type">Gene</infon>
|
|
230
|
-
<location offset="428" length="4"/>
|
|
231
|
-
<text>NDC1</text>
|
|
232
|
-
</annotation>
|
|
233
|
-
<annotation id="TEAM_277_2">
|
|
234
|
-
<infon key="type">Gene</infon>
|
|
235
|
-
<location offset="1197" length="6"/>
|
|
236
|
-
<text>POM152</text>
|
|
237
|
-
</annotation>
|
|
238
|
-
<annotation id="TEAM_277_3">
|
|
239
|
-
<infon key="type">Gene</infon>
|
|
240
|
-
<location offset="1334" length="4"/>
|
|
241
|
-
<text>NDC1</text>
|
|
242
|
-
</annotation>
|
|
243
|
-
<annotation id="0">
|
|
244
|
-
<infon key="type">ExperimentalMethod</infon>
|
|
245
|
-
<infon key="PSIMI">0416</infon>
|
|
246
|
-
<location offset="684" length="160"/>
|
|
247
|
-
<text>Indirect immunofluorescence microscopy shows that Ndc1p displays punctate, nuclear peripheral localization that colocalizes with a known NPC component, Nup49p.</text>
|
|
248
|
-
</annotation>
|
|
249
143
|
</passage>
|
|
250
144
|
<passage>
|
|
251
145
|
<infon key="type">paragraph</infon>
|
|
@@ -331,37 +225,6 @@
|
|
|
331
225
|
<location offset="2825" length="5"/>
|
|
332
226
|
<text>yeast</text>
|
|
333
227
|
</annotation>
|
|
334
|
-
<annotation id="G13">
|
|
335
|
-
<infon key="score">0.73</infon>
|
|
336
|
-
<infon key="GeneID">855159</infon>
|
|
337
|
-
<infon key="type">Gene</infon>
|
|
338
|
-
<location offset="2390" length="7"/>
|
|
339
|
-
<text>Pom152p</text>
|
|
340
|
-
</annotation>
|
|
341
|
-
<annotation id="G14">
|
|
342
|
-
<infon key="score">0.70</infon>
|
|
343
|
-
<infon key="GeneID">855159</infon>
|
|
344
|
-
<infon key="type">Gene</infon>
|
|
345
|
-
<location offset="2522" length="6"/>
|
|
346
|
-
<text>POM152</text>
|
|
347
|
-
</annotation>
|
|
348
|
-
<annotation id="G15">
|
|
349
|
-
<infon key="score">0.73</infon>
|
|
350
|
-
<infon key="GeneID">855159</infon>
|
|
351
|
-
<infon key="type">Gene</infon>
|
|
352
|
-
<location offset="2800" length="7"/>
|
|
353
|
-
<text>Pom152p</text>
|
|
354
|
-
</annotation>
|
|
355
|
-
<annotation id="TEAM_277_4">
|
|
356
|
-
<infon key="type">Gene</infon>
|
|
357
|
-
<location offset="2522" length="6"/>
|
|
358
|
-
<text>POM152</text>
|
|
359
|
-
</annotation>
|
|
360
|
-
<annotation id="TEAM_277_5">
|
|
361
|
-
<infon key="type">Gene</infon>
|
|
362
|
-
<location offset="2626" length="6"/>
|
|
363
|
-
<text>POM152</text>
|
|
364
|
-
</annotation>
|
|
365
228
|
</passage>
|
|
366
229
|
<passage>
|
|
367
230
|
<infon key="type">paragraph</infon>
|
|
@@ -414,42 +277,6 @@
|
|
|
414
277
|
<location offset="4612" length="5"/>
|
|
415
278
|
<text>Ndc1p</text>
|
|
416
279
|
</annotation>
|
|
417
|
-
<annotation id="G16">
|
|
418
|
-
<infon key="score">0.58</infon>
|
|
419
|
-
<infon key="GeneID">335236</infon>
|
|
420
|
-
<infon key="type">Gene</infon>
|
|
421
|
-
<location offset="3613" length="9"/>
|
|
422
|
-
<text>NDC1 gene</text>
|
|
423
|
-
</annotation>
|
|
424
|
-
<annotation id="G17">
|
|
425
|
-
<infon key="type">Gene</infon>
|
|
426
|
-
<location offset="3748" length="6"/>
|
|
427
|
-
<text>ndc1-1</text>
|
|
428
|
-
</annotation>
|
|
429
|
-
<annotation id="G18">
|
|
430
|
-
<infon key="score">0.70</infon>
|
|
431
|
-
<infon key="GeneID">335236</infon>
|
|
432
|
-
<infon key="type">Gene</infon>
|
|
433
|
-
<location offset="4470" length="4"/>
|
|
434
|
-
<text>NDC1</text>
|
|
435
|
-
</annotation>
|
|
436
|
-
<annotation id="G19">
|
|
437
|
-
<infon key="score">0.73</infon>
|
|
438
|
-
<infon key="GeneID">854977</infon>
|
|
439
|
-
<infon key="type">Gene</infon>
|
|
440
|
-
<location offset="4612" length="5"/>
|
|
441
|
-
<text>Ndc1p</text>
|
|
442
|
-
</annotation>
|
|
443
|
-
<annotation id="TEAM_277_6">
|
|
444
|
-
<infon key="type">Gene</infon>
|
|
445
|
-
<location offset="3613" length="4"/>
|
|
446
|
-
<text>NDC1</text>
|
|
447
|
-
</annotation>
|
|
448
|
-
<annotation id="TEAM_277_7">
|
|
449
|
-
<infon key="type">Gene</infon>
|
|
450
|
-
<location offset="4470" length="4"/>
|
|
451
|
-
<text>NDC1</text>
|
|
452
|
-
</annotation>
|
|
453
280
|
</passage>
|
|
454
281
|
<passage>
|
|
455
282
|
<infon key="type">paragraph</infon>
|
|
@@ -472,32 +299,6 @@
|
|
|
472
299
|
<location offset="4848" length="6"/>
|
|
473
300
|
<text>POM152</text>
|
|
474
301
|
</annotation>
|
|
475
|
-
<annotation id="G20">
|
|
476
|
-
<infon key="score">0.73</infon>
|
|
477
|
-
<infon key="GeneID">854977</infon>
|
|
478
|
-
<infon key="type">Gene</infon>
|
|
479
|
-
<location offset="4730" length="5"/>
|
|
480
|
-
<text>Ndc1p</text>
|
|
481
|
-
</annotation>
|
|
482
|
-
<annotation id="G21">
|
|
483
|
-
<infon key="score">0.70</infon>
|
|
484
|
-
<infon key="GeneID">855159</infon>
|
|
485
|
-
<infon key="type">Gene</infon>
|
|
486
|
-
<location offset="4848" length="6"/>
|
|
487
|
-
<text>POM152</text>
|
|
488
|
-
</annotation>
|
|
489
|
-
<annotation id="G22">
|
|
490
|
-
<infon key="score">0.70</infon>
|
|
491
|
-
<infon key="GeneID">100009194</infon>
|
|
492
|
-
<infon key="type">Gene</infon>
|
|
493
|
-
<location offset="4878" length="3"/>
|
|
494
|
-
<text>SPB</text>
|
|
495
|
-
</annotation>
|
|
496
|
-
<annotation id="TEAM_277_8">
|
|
497
|
-
<infon key="type">Gene</infon>
|
|
498
|
-
<location offset="4848" length="6"/>
|
|
499
|
-
<text>POM152</text>
|
|
500
|
-
</annotation>
|
|
501
302
|
</passage>
|
|
502
303
|
<passage>
|
|
503
304
|
<infon key="type">title_1</infon>
|
|
@@ -771,270 +572,6 @@
|
|
|
771
572
|
<location offset="5460" length="213"/>
|
|
772
573
|
<text>The ndc1-1, pom152 null strain [HC5-31c(1166); Table I] was constructed by replacing the entire POM152 open reading frame, plus an additional 163 bp after the stop codon, with the HIS3 gene (Baudin et al., 1993)</text>
|
|
773
574
|
</annotation>
|
|
774
|
-
<annotation id="G23">
|
|
775
|
-
<infon key="score">0.70</infon>
|
|
776
|
-
<infon key="GeneID">335236</infon>
|
|
777
|
-
<infon key="type">Gene</infon>
|
|
778
|
-
<location offset="5204" length="4"/>
|
|
779
|
-
<text>NDC1</text>
|
|
780
|
-
</annotation>
|
|
781
|
-
<annotation id="G24">
|
|
782
|
-
<infon key="score">0.70</infon>
|
|
783
|
-
<infon key="GeneID">7502638</infon>
|
|
784
|
-
<infon key="type">Gene</infon>
|
|
785
|
-
<location offset="5314" length="4"/>
|
|
786
|
-
<text>ProA</text>
|
|
787
|
-
</annotation>
|
|
788
|
-
<annotation id="G25">
|
|
789
|
-
<infon key="type">Gene</infon>
|
|
790
|
-
<location offset="5464" length="6"/>
|
|
791
|
-
<text>ndc1-1</text>
|
|
792
|
-
</annotation>
|
|
793
|
-
<annotation id="G26">
|
|
794
|
-
<infon key="score">0.70</infon>
|
|
795
|
-
<infon key="GeneID">855159</infon>
|
|
796
|
-
<infon key="type">Gene</infon>
|
|
797
|
-
<location offset="5472" length="6"/>
|
|
798
|
-
<text>pom152</text>
|
|
799
|
-
</annotation>
|
|
800
|
-
<annotation id="G27">
|
|
801
|
-
<infon key="score">0.75</infon>
|
|
802
|
-
<infon key="GeneID">2539698</infon>
|
|
803
|
-
<infon key="type">Gene</infon>
|
|
804
|
-
<location offset="5642" length="9"/>
|
|
805
|
-
<text>HIS3 gene</text>
|
|
806
|
-
</annotation>
|
|
807
|
-
<annotation id="G28">
|
|
808
|
-
<infon key="score">0.70</infon>
|
|
809
|
-
<infon key="GeneID">852703</infon>
|
|
810
|
-
<infon key="type">Gene</infon>
|
|
811
|
-
<location offset="5704" length="9"/>
|
|
812
|
-
<text>NUP49-GFP</text>
|
|
813
|
-
</annotation>
|
|
814
|
-
<annotation id="G29">
|
|
815
|
-
<infon key="type">Gene</infon>
|
|
816
|
-
<location offset="5847" length="7"/>
|
|
817
|
-
<text>nup49-1</text>
|
|
818
|
-
</annotation>
|
|
819
|
-
<annotation id="G30">
|
|
820
|
-
<infon key="type">Gene</infon>
|
|
821
|
-
<location offset="5865" length="14"/>
|
|
822
|
-
<text>nup49DeltaGFLG</text>
|
|
823
|
-
</annotation>
|
|
824
|
-
<annotation id="G31">
|
|
825
|
-
<infon key="score">0.70</infon>
|
|
826
|
-
<infon key="GeneID">852703</infon>
|
|
827
|
-
<infon key="type">Gene</infon>
|
|
828
|
-
<location offset="5927" length="9"/>
|
|
829
|
-
<text>NUP49-GFP</text>
|
|
830
|
-
</annotation>
|
|
831
|
-
<annotation id="G32">
|
|
832
|
-
<infon key="type">Gene</infon>
|
|
833
|
-
<location offset="5946" length="18"/>
|
|
834
|
-
<text>nup133 null allele</text>
|
|
835
|
-
</annotation>
|
|
836
|
-
<annotation id="G33">
|
|
837
|
-
<infon key="type">Gene</infon>
|
|
838
|
-
<location offset="6101" length="11"/>
|
|
839
|
-
<text>nup133Delta</text>
|
|
840
|
-
</annotation>
|
|
841
|
-
<annotation id="G34">
|
|
842
|
-
<infon key="score">0.75</infon>
|
|
843
|
-
<infon key="GeneID">2539698</infon>
|
|
844
|
-
<infon key="type">Gene</infon>
|
|
845
|
-
<location offset="6114" length="4"/>
|
|
846
|
-
<text>HIS3</text>
|
|
847
|
-
</annotation>
|
|
848
|
-
<annotation id="G35">
|
|
849
|
-
<infon key="type">Gene</infon>
|
|
850
|
-
<location offset="6120" length="7"/>
|
|
851
|
-
<text>nup49-1</text>
|
|
852
|
-
</annotation>
|
|
853
|
-
<annotation id="G36">
|
|
854
|
-
<infon key="type">Gene</infon>
|
|
855
|
-
<location offset="6139" length="14"/>
|
|
856
|
-
<text>nup49DeltaGFLG</text>
|
|
857
|
-
</annotation>
|
|
858
|
-
<annotation id="G37">
|
|
859
|
-
<infon key="type">Gene</infon>
|
|
860
|
-
<location offset="6197" length="10"/>
|
|
861
|
-
<text>NIC96-ProA</text>
|
|
862
|
-
</annotation>
|
|
863
|
-
<annotation id="G38">
|
|
864
|
-
<infon key="score">0.73</infon>
|
|
865
|
-
<infon key="GeneID">853824</infon>
|
|
866
|
-
<infon key="type">Gene</infon>
|
|
867
|
-
<location offset="6329" length="9"/>
|
|
868
|
-
<text>SPC42-GFP</text>
|
|
869
|
-
</annotation>
|
|
870
|
-
<annotation id="G39">
|
|
871
|
-
<infon key="type">Gene</infon>
|
|
872
|
-
<location offset="6355" length="12"/>
|
|
873
|
-
<text>HC23-11d/16a</text>
|
|
874
|
-
</annotation>
|
|
875
|
-
<annotation id="G40">
|
|
876
|
-
<infon key="score">0.70</infon>
|
|
877
|
-
<infon key="GeneID">54411</infon>
|
|
878
|
-
<infon key="type">Gene</infon>
|
|
879
|
-
<location offset="6364" length="3"/>
|
|
880
|
-
<text>16a</text>
|
|
881
|
-
</annotation>
|
|
882
|
-
<annotation id="G41">
|
|
883
|
-
<infon key="type">Gene</infon>
|
|
884
|
-
<location offset="6497" length="10"/>
|
|
885
|
-
<text>spc42Delta</text>
|
|
886
|
-
</annotation>
|
|
887
|
-
<annotation id="G42">
|
|
888
|
-
<infon key="score">0.59</infon>
|
|
889
|
-
<infon key="GeneID">668253</infon>
|
|
890
|
-
<infon key="type">Gene</infon>
|
|
891
|
-
<location offset="6509" length="4"/>
|
|
892
|
-
<text>LEU2</text>
|
|
893
|
-
</annotation>
|
|
894
|
-
<annotation id="G43">
|
|
895
|
-
<infon key="score">0.59</infon>
|
|
896
|
-
<infon key="GeneID">100342170</infon>
|
|
897
|
-
<infon key="type">Gene</infon>
|
|
898
|
-
<location offset="6519" length="4"/>
|
|
899
|
-
<text>TRP1</text>
|
|
900
|
-
</annotation>
|
|
901
|
-
<annotation id="G44">
|
|
902
|
-
<infon key="score">0.73</infon>
|
|
903
|
-
<infon key="GeneID">853824</infon>
|
|
904
|
-
<infon key="type">Gene</infon>
|
|
905
|
-
<location offset="6525" length="9"/>
|
|
906
|
-
<text>SPC42-GFP</text>
|
|
907
|
-
</annotation>
|
|
908
|
-
<annotation id="G45">
|
|
909
|
-
<infon key="score">0.70</infon>
|
|
910
|
-
<infon key="GeneID">335236</infon>
|
|
911
|
-
<infon key="type">Gene</infon>
|
|
912
|
-
<location offset="6649" length="4"/>
|
|
913
|
-
<text>NDC1</text>
|
|
914
|
-
</annotation>
|
|
915
|
-
<annotation id="G46">
|
|
916
|
-
<infon key="score">0.70</infon>
|
|
917
|
-
<infon key="GeneID">335236</infon>
|
|
918
|
-
<infon key="type">Gene</infon>
|
|
919
|
-
<location offset="6667" length="4"/>
|
|
920
|
-
<text>NDC1</text>
|
|
921
|
-
</annotation>
|
|
922
|
-
<annotation id="G47">
|
|
923
|
-
<infon key="type">Gene</infon>
|
|
924
|
-
<location offset="6723" length="9"/>
|
|
925
|
-
<text>ndc1Delta</text>
|
|
926
|
-
</annotation>
|
|
927
|
-
<annotation id="G48">
|
|
928
|
-
<infon key="type">Gene</infon>
|
|
929
|
-
<location offset="6734" length="16"/>
|
|
930
|
-
<text>HIS3 null allele</text>
|
|
931
|
-
</annotation>
|
|
932
|
-
<annotation id="G49">
|
|
933
|
-
<infon key="score">0.70</infon>
|
|
934
|
-
<infon key="GeneID">2539698</infon>
|
|
935
|
-
<infon key="type">Gene</infon>
|
|
936
|
-
<location offset="6823" length="9"/>
|
|
937
|
-
<text>HIS3 gene</text>
|
|
938
|
-
</annotation>
|
|
939
|
-
<annotation id="G50">
|
|
940
|
-
<infon key="type">Gene</infon>
|
|
941
|
-
<location offset="6860" length="9"/>
|
|
942
|
-
<text>ndc1Delta</text>
|
|
943
|
-
</annotation>
|
|
944
|
-
<annotation id="G51">
|
|
945
|
-
<infon key="type">Gene</infon>
|
|
946
|
-
<location offset="6872" length="17"/>
|
|
947
|
-
<text>KANMX null allele</text>
|
|
948
|
-
</annotation>
|
|
949
|
-
<annotation id="G52">
|
|
950
|
-
<infon key="score">0.70</infon>
|
|
951
|
-
<infon key="GeneID">335236</infon>
|
|
952
|
-
<infon key="type">Gene</infon>
|
|
953
|
-
<location offset="6987" length="4"/>
|
|
954
|
-
<text>NDC1</text>
|
|
955
|
-
</annotation>
|
|
956
|
-
<annotation id="G53">
|
|
957
|
-
<infon key="type">Gene</infon>
|
|
958
|
-
<location offset="7034" length="10"/>
|
|
959
|
-
<text>KANMX gene</text>
|
|
960
|
-
</annotation>
|
|
961
|
-
<annotation id="TEAM_277_9">
|
|
962
|
-
<infon key="type">Gene</infon>
|
|
963
|
-
<location offset="5204" length="4"/>
|
|
964
|
-
<text>NDC1</text>
|
|
965
|
-
</annotation>
|
|
966
|
-
<annotation id="TEAM_277_10">
|
|
967
|
-
<infon key="type">Gene</infon>
|
|
968
|
-
<location offset="5280" length="32"/>
|
|
969
|
-
<text>IgG binding domains of protein A</text>
|
|
970
|
-
</annotation>
|
|
971
|
-
<annotation id="TEAM_277_11">
|
|
972
|
-
<infon key="type">Gene</infon>
|
|
973
|
-
<location offset="5557" length="6"/>
|
|
974
|
-
<text>POM152</text>
|
|
975
|
-
</annotation>
|
|
976
|
-
<annotation id="TEAM_277_12">
|
|
977
|
-
<infon key="type">Gene</infon>
|
|
978
|
-
<infon key="GeneID">393202</infon>
|
|
979
|
-
<location offset="5642" length="4"/>
|
|
980
|
-
<text>HIS3</text>
|
|
981
|
-
</annotation>
|
|
982
|
-
<annotation id="TEAM_277_13">
|
|
983
|
-
<infon key="type">Gene</infon>
|
|
984
|
-
<infon key="GeneID">393202</infon>
|
|
985
|
-
<location offset="6114" length="4"/>
|
|
986
|
-
<text>HIS3</text>
|
|
987
|
-
</annotation>
|
|
988
|
-
<annotation id="TEAM_277_14">
|
|
989
|
-
<infon key="type">Gene</infon>
|
|
990
|
-
<infon key="GeneID">13745</infon>
|
|
991
|
-
<location offset="6129" length="4"/>
|
|
992
|
-
<text>URA3</text>
|
|
993
|
-
</annotation>
|
|
994
|
-
<annotation id="TEAM_277_15">
|
|
995
|
-
<infon key="type">Gene</infon>
|
|
996
|
-
<infon key="GeneID">9980</infon>
|
|
997
|
-
<location offset="6509" length="4"/>
|
|
998
|
-
<text>LEU2</text>
|
|
999
|
-
</annotation>
|
|
1000
|
-
<annotation id="TEAM_277_16">
|
|
1001
|
-
<infon key="type">Gene</infon>
|
|
1002
|
-
<location offset="6519" length="4"/>
|
|
1003
|
-
<text>TRP1</text>
|
|
1004
|
-
</annotation>
|
|
1005
|
-
<annotation id="TEAM_277_17">
|
|
1006
|
-
<infon key="type">Gene</infon>
|
|
1007
|
-
<location offset="6649" length="4"/>
|
|
1008
|
-
<text>NDC1</text>
|
|
1009
|
-
</annotation>
|
|
1010
|
-
<annotation id="TEAM_277_18">
|
|
1011
|
-
<infon key="type">Gene</infon>
|
|
1012
|
-
<location offset="6667" length="4"/>
|
|
1013
|
-
<text>NDC1</text>
|
|
1014
|
-
</annotation>
|
|
1015
|
-
<annotation id="TEAM_277_19">
|
|
1016
|
-
<infon key="type">Gene</infon>
|
|
1017
|
-
<infon key="GeneID">393202</infon>
|
|
1018
|
-
<location offset="6734" length="4"/>
|
|
1019
|
-
<text>HIS3</text>
|
|
1020
|
-
</annotation>
|
|
1021
|
-
<annotation id="TEAM_277_20">
|
|
1022
|
-
<infon key="type">Gene</infon>
|
|
1023
|
-
<infon key="GeneID">393202</infon>
|
|
1024
|
-
<location offset="6823" length="4"/>
|
|
1025
|
-
<text>HIS3</text>
|
|
1026
|
-
</annotation>
|
|
1027
|
-
<annotation id="TEAM_277_21">
|
|
1028
|
-
<infon key="type">Gene</infon>
|
|
1029
|
-
<location offset="6987" length="4"/>
|
|
1030
|
-
<text>NDC1</text>
|
|
1031
|
-
</annotation>
|
|
1032
|
-
<annotation id="TEAM_277_22">
|
|
1033
|
-
<infon key="type">Gene</infon>
|
|
1034
|
-
<infon key="GeneID">271781</infon>
|
|
1035
|
-
<location offset="7034" length="5"/>
|
|
1036
|
-
<text>KANMX</text>
|
|
1037
|
-
</annotation>
|
|
1038
575
|
</passage>
|
|
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|
<passage>
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|
<infon key="type">paragraph</infon>
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|
@@ -1260,106 +797,6 @@
|
|
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|
<location offset="8478" length="314"/>
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|
<text>The pRS315-NDC1-green fluorescent protein (GFP) plasmid was constructed by subcloning a PCR fragment containing GFP amplified from the pyEGFP3 plasmid (Cormack et al., 1997) with XbaI restriction sites into an AvrII linker introduced at the end of NDC1 cloned into the pRS315 vector (Sikorski and Hieter, 1989)</text>
|
|
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|
</annotation>
|
|
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|
-
<annotation id="G54">
|
|
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|
-
<infon key="score">0.70</infon>
|
|
1265
|
-
<infon key="GeneID">335236</infon>
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|
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|
-
<infon key="type">Gene</infon>
|
|
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|
-
<location offset="7961" length="13"/>
|
|
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|
-
<text>NDC1 fragment</text>
|
|
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|
-
</annotation>
|
|
1270
|
-
<annotation id="G55">
|
|
1271
|
-
<infon key="score">0.70</infon>
|
|
1272
|
-
<infon key="GeneID">55811</infon>
|
|
1273
|
-
<infon key="type">Gene</infon>
|
|
1274
|
-
<location offset="8006" length="10"/>
|
|
1275
|
-
<text>SacI sites</text>
|
|
1276
|
-
</annotation>
|
|
1277
|
-
<annotation id="G56">
|
|
1278
|
-
<infon key="type">Gene</infon>
|
|
1279
|
-
<location offset="8171" length="4"/>
|
|
1280
|
-
<text>URA3</text>
|
|
1281
|
-
</annotation>
|
|
1282
|
-
<annotation id="G57">
|
|
1283
|
-
<infon key="score">0.70</infon>
|
|
1284
|
-
<infon key="GeneID">33986</infon>
|
|
1285
|
-
<infon key="type">Gene</infon>
|
|
1286
|
-
<location offset="8180" length="4"/>
|
|
1287
|
-
<text>ADE3</text>
|
|
1288
|
-
</annotation>
|
|
1289
|
-
<annotation id="G58">
|
|
1290
|
-
<infon key="type">Gene</infon>
|
|
1291
|
-
<location offset="8346" length="11"/>
|
|
1292
|
-
<text>BglII sites</text>
|
|
1293
|
-
</annotation>
|
|
1294
|
-
<annotation id="G59">
|
|
1295
|
-
<infon key="type">Gene</infon>
|
|
1296
|
-
<location offset="8386" length="10"/>
|
|
1297
|
-
<text>BamHI site</text>
|
|
1298
|
-
</annotation>
|
|
1299
|
-
<annotation id="G60">
|
|
1300
|
-
<infon key="type">Gene</infon>
|
|
1301
|
-
<location offset="8435" length="13"/>
|
|
1302
|
-
<text>GAL1 promoter</text>
|
|
1303
|
-
</annotation>
|
|
1304
|
-
<annotation id="G61">
|
|
1305
|
-
<infon key="type">Gene</infon>
|
|
1306
|
-
<location offset="8482" length="37"/>
|
|
1307
|
-
<text>pRS315-NDC1-green fluorescent protein</text>
|
|
1308
|
-
</annotation>
|
|
1309
|
-
<annotation id="G62">
|
|
1310
|
-
<infon key="score">0.73</infon>
|
|
1311
|
-
<infon key="GeneID">7011691</infon>
|
|
1312
|
-
<infon key="type">Gene</infon>
|
|
1313
|
-
<location offset="8521" length="3"/>
|
|
1314
|
-
<text>GFP</text>
|
|
1315
|
-
</annotation>
|
|
1316
|
-
<annotation id="G63">
|
|
1317
|
-
<infon key="score">0.73</infon>
|
|
1318
|
-
<infon key="GeneID">7011691</infon>
|
|
1319
|
-
<infon key="type">Gene</infon>
|
|
1320
|
-
<location offset="8591" length="3"/>
|
|
1321
|
-
<text>GFP</text>
|
|
1322
|
-
</annotation>
|
|
1323
|
-
<annotation id="G64">
|
|
1324
|
-
<infon key="type">Gene</infon>
|
|
1325
|
-
<location offset="8659" length="23"/>
|
|
1326
|
-
<text>XbaI restriction sites</text>
|
|
1327
|
-
</annotation>
|
|
1328
|
-
<annotation id="G65">
|
|
1329
|
-
<infon key="score">0.70</infon>
|
|
1330
|
-
<infon key="GeneID">335236</infon>
|
|
1331
|
-
<infon key="type">Gene</infon>
|
|
1332
|
-
<location offset="8729" length="4"/>
|
|
1333
|
-
<text>NDC1</text>
|
|
1334
|
-
</annotation>
|
|
1335
|
-
<annotation id="TEAM_277_23">
|
|
1336
|
-
<infon key="type">Gene</infon>
|
|
1337
|
-
<location offset="7961" length="4"/>
|
|
1338
|
-
<text>NDC1</text>
|
|
1339
|
-
</annotation>
|
|
1340
|
-
<annotation id="TEAM_277_24">
|
|
1341
|
-
<infon key="type">Gene</infon>
|
|
1342
|
-
<infon key="GeneID">13745</infon>
|
|
1343
|
-
<location offset="8171" length="4"/>
|
|
1344
|
-
<text>URA3</text>
|
|
1345
|
-
</annotation>
|
|
1346
|
-
<annotation id="TEAM_277_25">
|
|
1347
|
-
<infon key="type">Gene</infon>
|
|
1348
|
-
<infon key="GeneID">11840</infon>
|
|
1349
|
-
<location offset="8435" length="4"/>
|
|
1350
|
-
<text>GAL1</text>
|
|
1351
|
-
</annotation>
|
|
1352
|
-
<annotation id="TEAM_277_26">
|
|
1353
|
-
<infon key="type">Gene</infon>
|
|
1354
|
-
<location offset="8729" length="4"/>
|
|
1355
|
-
<text>NDC1</text>
|
|
1356
|
-
</annotation>
|
|
1357
|
-
<annotation id="TEAM_277_27">
|
|
1358
|
-
<infon key="type">Gene</infon>
|
|
1359
|
-
<infon key="GeneID">12205</infon>
|
|
1360
|
-
<location offset="8751" length="6"/>
|
|
1361
|
-
<text>pRS315</text>
|
|
1362
|
-
</annotation>
|
|
1363
800
|
</passage>
|
|
1364
801
|
<passage>
|
|
1365
802
|
<infon key="type">title_2</infon>
|
|
@@ -1406,58 +843,6 @@
|
|
|
1406
843
|
<location offset="9666" length="6"/>
|
|
1407
844
|
<text>Nup57p</text>
|
|
1408
845
|
</annotation>
|
|
1409
|
-
<annotation id="G66">
|
|
1410
|
-
<infon key="type">Gene</infon>
|
|
1411
|
-
<location offset="8895" length="9"/>
|
|
1412
|
-
<text>Kilmartin</text>
|
|
1413
|
-
</annotation>
|
|
1414
|
-
<annotation id="G67">
|
|
1415
|
-
<infon key="type">Gene</infon>
|
|
1416
|
-
<location offset="8921" length="9"/>
|
|
1417
|
-
<text>Kilmartin</text>
|
|
1418
|
-
</annotation>
|
|
1419
|
-
<annotation id="G68">
|
|
1420
|
-
<infon key="score">0.73</infon>
|
|
1421
|
-
<infon key="GeneID">855159</infon>
|
|
1422
|
-
<infon key="type">Gene</infon>
|
|
1423
|
-
<location offset="9315" length="7"/>
|
|
1424
|
-
<text>Pom152p</text>
|
|
1425
|
-
</annotation>
|
|
1426
|
-
<annotation id="G69">
|
|
1427
|
-
<infon key="type">Gene</infon>
|
|
1428
|
-
<location offset="9422" length="9"/>
|
|
1429
|
-
<text>mAb 118C3</text>
|
|
1430
|
-
</annotation>
|
|
1431
|
-
<annotation id="G70">
|
|
1432
|
-
<infon key="score">0.73</infon>
|
|
1433
|
-
<infon key="GeneID">851957</infon>
|
|
1434
|
-
<infon key="type">Gene</infon>
|
|
1435
|
-
<location offset="9511" length="7"/>
|
|
1436
|
-
<text>Spc110p</text>
|
|
1437
|
-
</annotation>
|
|
1438
|
-
<annotation id="G71">
|
|
1439
|
-
<infon key="type">Gene</infon>
|
|
1440
|
-
<location offset="9648" length="9"/>
|
|
1441
|
-
<text>Kilmartin</text>
|
|
1442
|
-
</annotation>
|
|
1443
|
-
<annotation id="G72">
|
|
1444
|
-
<infon key="score">0.73</infon>
|
|
1445
|
-
<infon key="GeneID">853016</infon>
|
|
1446
|
-
<infon key="type">Gene</infon>
|
|
1447
|
-
<location offset="9666" length="6"/>
|
|
1448
|
-
<text>Nup57p</text>
|
|
1449
|
-
</annotation>
|
|
1450
|
-
<annotation id="TEAM_277_28">
|
|
1451
|
-
<infon key="type">Gene</infon>
|
|
1452
|
-
<location offset="9422" length="9"/>
|
|
1453
|
-
<text>mAb 118C3</text>
|
|
1454
|
-
</annotation>
|
|
1455
|
-
<annotation id="TEAM_277_29">
|
|
1456
|
-
<infon key="type">Gene</infon>
|
|
1457
|
-
<infon key="GeneID">683083</infon>
|
|
1458
|
-
<location offset="9705" length="7"/>
|
|
1459
|
-
<text>mAb 414</text>
|
|
1460
|
-
</annotation>
|
|
1461
846
|
</passage>
|
|
1462
847
|
<passage>
|
|
1463
848
|
<infon key="type">title_2</infon>
|
|
@@ -1509,12 +894,6 @@
|
|
|
1509
894
|
<location offset="10180" length="8"/>
|
|
1510
895
|
<text>Tween 20</text>
|
|
1511
896
|
</annotation>
|
|
1512
|
-
<annotation id="1">
|
|
1513
|
-
<infon key="type">ExperimentalMethod</infon>
|
|
1514
|
-
<infon key="PSIMI">0416</infon>
|
|
1515
|
-
<location offset="9849" length="341"/>
|
|
1516
|
-
<text>Buffer solutions used for immunofluorescence (IF) microscopy include PBSA (10 mg/ml NaCl, 0.2 mg/ml KCl, 1.43 mg/ml KH2PO4), solution A (1.2 M sorbitol, 100 mM KPO4, pH 7.5), spheroplasting solution (solution A containing 4.83 mug/ml Zymolyase 100T, 0.1 M beta-mercaptoethanol), and blocker (PBSA containing 10 mg/ml BSA, 0.1% Tween 20).</text>
|
|
1517
|
-
</annotation>
|
|
1518
897
|
</passage>
|
|
1519
898
|
<passage>
|
|
1520
899
|
<infon key="type">paragraph</infon>
|
|
@@ -1592,40 +971,11 @@
|
|
|
1592
971
|
<location offset="11171" length="6"/>
|
|
1593
972
|
<text>rabbit</text>
|
|
1594
973
|
</annotation>
|
|
1595
|
-
<annotation id="G73">
|
|
1596
|
-
<infon key="score">0.70</infon>
|
|
1597
|
-
<infon key="GeneID">7502638</infon>
|
|
1598
|
-
<infon key="type">Gene</infon>
|
|
1599
|
-
<location offset="10262" length="4"/>
|
|
1600
|
-
<text>ProA</text>
|
|
1601
|
-
</annotation>
|
|
1602
|
-
<annotation id="G74">
|
|
1603
|
-
<infon key="type">Gene</infon>
|
|
1604
|
-
<location offset="11140" length="43"/>
|
|
1605
|
-
<text>Texas red-conjugated goat anti-rabbit IgGs</text>
|
|
1606
|
-
</annotation>
|
|
1607
|
-
<annotation id="TEAM_277_30">
|
|
1608
|
-
<infon key="type">Gene</infon>
|
|
1609
|
-
<location offset="10239" length="19"/>
|
|
1610
|
-
<text>IgG binding domains</text>
|
|
1611
|
-
</annotation>
|
|
1612
|
-
<annotation id="TEAM_277_31">
|
|
1613
|
-
<infon key="type">Gene</infon>
|
|
1614
|
-
<infon key="GeneID">237249</infon>
|
|
1615
|
-
<location offset="10262" length="4"/>
|
|
1616
|
-
<text>ProA</text>
|
|
1617
|
-
</annotation>
|
|
1618
974
|
</passage>
|
|
1619
975
|
<passage>
|
|
1620
976
|
<infon key="type">paragraph</infon>
|
|
1621
977
|
<offset>11465</offset>
|
|
1622
978
|
<text>Standard fluorescence microscopy was carried out using a Zeiss fluorescence microscope equipped with an Empix CCD camera. Images were captured using MetaMorph imaging software (Universal Imaging, West Chester, PA). Deconvolution microscopy was carried out using a Leica DMRXA/RF4/V automated universal microscope equipped with a Cooke SensiCam high performance digital camera. Images were acquired and deconvolved using the Slidebook software package (Intelligent Imaging Innovations, Denver, CO).</text>
|
|
1623
|
-
<annotation id="2">
|
|
1624
|
-
<infon key="type">ExperimentalMethod</infon>
|
|
1625
|
-
<infon key="PSIMI">0416</infon>
|
|
1626
|
-
<location offset="11465" length="121"/>
|
|
1627
|
-
<text>Standard fluorescence microscopy was carried out using a Zeiss fluorescence microscope equipped with an Empix CCD camera.</text>
|
|
1628
|
-
</annotation>
|
|
1629
979
|
</passage>
|
|
1630
980
|
<passage>
|
|
1631
981
|
<infon key="type">paragraph</infon>
|
|
@@ -1821,16 +1171,6 @@
|
|
|
1821
1171
|
<location offset="14274" length="5"/>
|
|
1822
1172
|
<text>yeast</text>
|
|
1823
1173
|
</annotation>
|
|
1824
|
-
<annotation id="G75">
|
|
1825
|
-
<infon key="type">Gene</infon>
|
|
1826
|
-
<location offset="13631" length="12"/>
|
|
1827
|
-
<text>HC10-42a/42d</text>
|
|
1828
|
-
</annotation>
|
|
1829
|
-
<annotation id="G76">
|
|
1830
|
-
<infon key="type">Gene</infon>
|
|
1831
|
-
<location offset="13640" length="3"/>
|
|
1832
|
-
<text>42d</text>
|
|
1833
|
-
</annotation>
|
|
1834
1174
|
</passage>
|
|
1835
1175
|
<passage>
|
|
1836
1176
|
<infon key="type">title_1</infon>
|
|
@@ -1847,13 +1187,6 @@
|
|
|
1847
1187
|
<location offset="14332" length="5"/>
|
|
1848
1188
|
<text>Ndc1p</text>
|
|
1849
1189
|
</annotation>
|
|
1850
|
-
<annotation id="G77">
|
|
1851
|
-
<infon key="score">0.73</infon>
|
|
1852
|
-
<infon key="GeneID">854977</infon>
|
|
1853
|
-
<infon key="type">Gene</infon>
|
|
1854
|
-
<location offset="14332" length="5"/>
|
|
1855
|
-
<text>Ndc1p</text>
|
|
1856
|
-
</annotation>
|
|
1857
1190
|
</passage>
|
|
1858
1191
|
<passage>
|
|
1859
1192
|
<infon key="type">paragraph</infon>
|
|
@@ -1895,20 +1228,6 @@
|
|
|
1895
1228
|
<location offset="14908" length="5"/>
|
|
1896
1229
|
<text>Ndc1p</text>
|
|
1897
1230
|
</annotation>
|
|
1898
|
-
<annotation id="G78">
|
|
1899
|
-
<infon key="score">0.73</infon>
|
|
1900
|
-
<infon key="GeneID">854977</infon>
|
|
1901
|
-
<infon key="type">Gene</infon>
|
|
1902
|
-
<location offset="14709" length="5"/>
|
|
1903
|
-
<text>Ndc1p</text>
|
|
1904
|
-
</annotation>
|
|
1905
|
-
<annotation id="G79">
|
|
1906
|
-
<infon key="score">0.73</infon>
|
|
1907
|
-
<infon key="GeneID">854977</infon>
|
|
1908
|
-
<infon key="type">Gene</infon>
|
|
1909
|
-
<location offset="14875" length="5"/>
|
|
1910
|
-
<text>Ndc1p</text>
|
|
1911
|
-
</annotation>
|
|
1912
1231
|
</passage>
|
|
1913
1232
|
<passage>
|
|
1914
1233
|
<infon key="type">paragraph</infon>
|
|
@@ -1968,95 +1287,6 @@
|
|
|
1968
1287
|
<location offset="15644" length="4"/>
|
|
1969
1288
|
<text>NDC1</text>
|
|
1970
1289
|
</annotation>
|
|
1971
|
-
<annotation id="G80">
|
|
1972
|
-
<infon key="score">0.73</infon>
|
|
1973
|
-
<infon key="GeneID">854977</infon>
|
|
1974
|
-
<infon key="type">Gene</infon>
|
|
1975
|
-
<location offset="14961" length="5"/>
|
|
1976
|
-
<text>Ndc1p</text>
|
|
1977
|
-
</annotation>
|
|
1978
|
-
<annotation id="G81">
|
|
1979
|
-
<infon key="score">0.70</infon>
|
|
1980
|
-
<infon key="GeneID">335236</infon>
|
|
1981
|
-
<infon key="type">Gene</infon>
|
|
1982
|
-
<location offset="15034" length="4"/>
|
|
1983
|
-
<text>NDC1</text>
|
|
1984
|
-
</annotation>
|
|
1985
|
-
<annotation id="G82">
|
|
1986
|
-
<infon key="score">0.70</infon>
|
|
1987
|
-
<infon key="GeneID">7502638</infon>
|
|
1988
|
-
<infon key="type">Gene</infon>
|
|
1989
|
-
<location offset="15109" length="4"/>
|
|
1990
|
-
<text>ProA</text>
|
|
1991
|
-
</annotation>
|
|
1992
|
-
<annotation id="G83">
|
|
1993
|
-
<infon key="score">0.70</infon>
|
|
1994
|
-
<infon key="GeneID">335236</infon>
|
|
1995
|
-
<infon key="type">Gene</infon>
|
|
1996
|
-
<location offset="15158" length="4"/>
|
|
1997
|
-
<text>NDC1</text>
|
|
1998
|
-
</annotation>
|
|
1999
|
-
<annotation id="G84">
|
|
2000
|
-
<infon key="score">0.70</infon>
|
|
2001
|
-
<infon key="GeneID">335236</infon>
|
|
2002
|
-
<infon key="type">Gene</infon>
|
|
2003
|
-
<location offset="15224" length="4"/>
|
|
2004
|
-
<text>NDC1</text>
|
|
2005
|
-
</annotation>
|
|
2006
|
-
<annotation id="G85">
|
|
2007
|
-
<infon key="type">Gene</infon>
|
|
2008
|
-
<location offset="15264" length="13"/>
|
|
2009
|
-
<text>NDC1 promoter</text>
|
|
2010
|
-
</annotation>
|
|
2011
|
-
<annotation id="G86">
|
|
2012
|
-
<infon key="type">Gene</infon>
|
|
2013
|
-
<location offset="15398" length="10"/>
|
|
2014
|
-
<text>Ndc1p-ProA</text>
|
|
2015
|
-
</annotation>
|
|
2016
|
-
<annotation id="G87">
|
|
2017
|
-
<infon key="score">0.70</infon>
|
|
2018
|
-
<infon key="GeneID">335236</infon>
|
|
2019
|
-
<infon key="type">Gene</infon>
|
|
2020
|
-
<location offset="15644" length="4"/>
|
|
2021
|
-
<text>NDC1</text>
|
|
2022
|
-
</annotation>
|
|
2023
|
-
<annotation id="G88">
|
|
2024
|
-
<infon key="score">0.70</infon>
|
|
2025
|
-
<infon key="GeneID">100327265</infon>
|
|
2026
|
-
<infon key="type">Gene</infon>
|
|
2027
|
-
<location offset="15685" length="5"/>
|
|
2028
|
-
<text>c-myc</text>
|
|
2029
|
-
</annotation>
|
|
2030
|
-
<annotation id="TEAM_277_32">
|
|
2031
|
-
<infon key="type">Gene</infon>
|
|
2032
|
-
<location offset="15034" length="4"/>
|
|
2033
|
-
<text>NDC1</text>
|
|
2034
|
-
</annotation>
|
|
2035
|
-
<annotation id="TEAM_277_33">
|
|
2036
|
-
<infon key="type">Gene</infon>
|
|
2037
|
-
<location offset="15085" length="28"/>
|
|
2038
|
-
<text>IgG binding domains of ProA</text>
|
|
2039
|
-
</annotation>
|
|
2040
|
-
<annotation id="TEAM_277_34">
|
|
2041
|
-
<infon key="type">Gene</infon>
|
|
2042
|
-
<location offset="15158" length="4"/>
|
|
2043
|
-
<text>NDC1</text>
|
|
2044
|
-
</annotation>
|
|
2045
|
-
<annotation id="TEAM_277_35">
|
|
2046
|
-
<infon key="type">Gene</infon>
|
|
2047
|
-
<location offset="15224" length="4"/>
|
|
2048
|
-
<text>NDC1</text>
|
|
2049
|
-
</annotation>
|
|
2050
|
-
<annotation id="TEAM_277_36">
|
|
2051
|
-
<infon key="type">Gene</infon>
|
|
2052
|
-
<location offset="15264" length="4"/>
|
|
2053
|
-
<text>NDC1</text>
|
|
2054
|
-
</annotation>
|
|
2055
|
-
<annotation id="TEAM_277_37">
|
|
2056
|
-
<infon key="type">Gene</infon>
|
|
2057
|
-
<location offset="15644" length="4"/>
|
|
2058
|
-
<text>NDC1</text>
|
|
2059
|
-
</annotation>
|
|
2060
1290
|
</passage>
|
|
2061
1291
|
<passage>
|
|
2062
1292
|
<infon key="type">paragraph</infon>
|
|
@@ -2086,85 +1316,6 @@
|
|
|
2086
1316
|
<location offset="16349" length="6"/>
|
|
2087
1317
|
<text>nup133</text>
|
|
2088
1318
|
</annotation>
|
|
2089
|
-
<annotation id="G89">
|
|
2090
|
-
<infon key="score">0.73</infon>
|
|
2091
|
-
<infon key="GeneID">852703</infon>
|
|
2092
|
-
<infon key="type">Gene</infon>
|
|
2093
|
-
<location offset="15815" length="6"/>
|
|
2094
|
-
<text>Nup49p</text>
|
|
2095
|
-
</annotation>
|
|
2096
|
-
<annotation id="G90">
|
|
2097
|
-
<infon key="score">0.70</infon>
|
|
2098
|
-
<infon key="GeneID">852703</infon>
|
|
2099
|
-
<infon key="type">Gene</infon>
|
|
2100
|
-
<location offset="15907" length="5"/>
|
|
2101
|
-
<text>NUP49</text>
|
|
2102
|
-
</annotation>
|
|
2103
|
-
<annotation id="G91">
|
|
2104
|
-
<infon key="score">0.73</infon>
|
|
2105
|
-
<infon key="GeneID">852703</infon>
|
|
2106
|
-
<infon key="type">Gene</infon>
|
|
2107
|
-
<location offset="16080" length="10"/>
|
|
2108
|
-
<text>Nup49p-GFP</text>
|
|
2109
|
-
</annotation>
|
|
2110
|
-
<annotation id="G92">
|
|
2111
|
-
<infon key="score">0.73</infon>
|
|
2112
|
-
<infon key="GeneID">852703</infon>
|
|
2113
|
-
<infon key="type">Gene</infon>
|
|
2114
|
-
<location offset="16187" length="10"/>
|
|
2115
|
-
<text>Nup49p-GFP</text>
|
|
2116
|
-
</annotation>
|
|
2117
|
-
<annotation id="G93">
|
|
2118
|
-
<infon key="score">0.70</infon>
|
|
2119
|
-
<infon key="GeneID">7502638</infon>
|
|
2120
|
-
<infon key="type">Gene</infon>
|
|
2121
|
-
<location offset="16211" length="12"/>
|
|
2122
|
-
<text>ProA epitope</text>
|
|
2123
|
-
</annotation>
|
|
2124
|
-
<annotation id="G94">
|
|
2125
|
-
<infon key="score">0.73</infon>
|
|
2126
|
-
<infon key="GeneID">854977</infon>
|
|
2127
|
-
<infon key="type">Gene</infon>
|
|
2128
|
-
<location offset="16227" length="5"/>
|
|
2129
|
-
<text>Ndc1p</text>
|
|
2130
|
-
</annotation>
|
|
2131
|
-
<annotation id="G95">
|
|
2132
|
-
<infon key="score">0.58</infon>
|
|
2133
|
-
<infon key="GeneID">406852</infon>
|
|
2134
|
-
<infon key="type">Gene</infon>
|
|
2135
|
-
<location offset="16349" length="6"/>
|
|
2136
|
-
<text>nup133</text>
|
|
2137
|
-
</annotation>
|
|
2138
|
-
<annotation id="G96">
|
|
2139
|
-
<infon key="score">0.73</infon>
|
|
2140
|
-
<infon key="GeneID">852703</infon>
|
|
2141
|
-
<infon key="type">Gene</infon>
|
|
2142
|
-
<location offset="16520" length="10"/>
|
|
2143
|
-
<text>Nup49p-GFP</text>
|
|
2144
|
-
</annotation>
|
|
2145
|
-
<annotation id="G97">
|
|
2146
|
-
<infon key="type">Gene</infon>
|
|
2147
|
-
<location offset="16582" length="10"/>
|
|
2148
|
-
<text>Ndc1p-ProA</text>
|
|
2149
|
-
</annotation>
|
|
2150
|
-
<annotation id="G98">
|
|
2151
|
-
<infon key="score">0.73</infon>
|
|
2152
|
-
<infon key="GeneID">852703</infon>
|
|
2153
|
-
<infon key="type">Gene</infon>
|
|
2154
|
-
<location offset="16759" length="10"/>
|
|
2155
|
-
<text>Nup49p-GFP</text>
|
|
2156
|
-
</annotation>
|
|
2157
|
-
<annotation id="TEAM_277_38">
|
|
2158
|
-
<infon key="type">Gene</infon>
|
|
2159
|
-
<infon key="GeneID">4415</infon>
|
|
2160
|
-
<location offset="15907" length="5"/>
|
|
2161
|
-
<text>NUP49</text>
|
|
2162
|
-
</annotation>
|
|
2163
|
-
<annotation id="TEAM_277_39">
|
|
2164
|
-
<infon key="type">Gene</infon>
|
|
2165
|
-
<location offset="16520" length="10"/>
|
|
2166
|
-
<text>Nup49p-GFP</text>
|
|
2167
|
-
</annotation>
|
|
2168
1319
|
</passage>
|
|
2169
1320
|
<passage>
|
|
2170
1321
|
<infon key="type">paragraph</infon>
|
|
@@ -2217,56 +1368,6 @@
|
|
|
2217
1368
|
<location offset="17555" length="7"/>
|
|
2218
1369
|
<text>Pom152p</text>
|
|
2219
1370
|
</annotation>
|
|
2220
|
-
<annotation id="G99">
|
|
2221
|
-
<infon key="type">Gene</infon>
|
|
2222
|
-
<location offset="16899" length="10"/>
|
|
2223
|
-
<text>Ndc1p-ProA</text>
|
|
2224
|
-
</annotation>
|
|
2225
|
-
<annotation id="G100">
|
|
2226
|
-
<infon key="score">0.73</infon>
|
|
2227
|
-
<infon key="GeneID">855159</infon>
|
|
2228
|
-
<infon key="type">Gene</infon>
|
|
2229
|
-
<location offset="16980" length="7"/>
|
|
2230
|
-
<text>Pom152p</text>
|
|
2231
|
-
</annotation>
|
|
2232
|
-
<annotation id="G101">
|
|
2233
|
-
<infon key="score">0.73</infon>
|
|
2234
|
-
<infon key="GeneID">854977</infon>
|
|
2235
|
-
<infon key="type">Gene</infon>
|
|
2236
|
-
<location offset="17237" length="5"/>
|
|
2237
|
-
<text>Ndc1p</text>
|
|
2238
|
-
</annotation>
|
|
2239
|
-
<annotation id="G102">
|
|
2240
|
-
<infon key="score">0.73</infon>
|
|
2241
|
-
<infon key="GeneID">853016</infon>
|
|
2242
|
-
<infon key="type">Gene</infon>
|
|
2243
|
-
<location offset="17493" length="6"/>
|
|
2244
|
-
<text>Nup57p</text>
|
|
2245
|
-
</annotation>
|
|
2246
|
-
<annotation id="G103">
|
|
2247
|
-
<infon key="score">0.73</infon>
|
|
2248
|
-
<infon key="GeneID">855159</infon>
|
|
2249
|
-
<infon key="type">Gene</infon>
|
|
2250
|
-
<location offset="17555" length="7"/>
|
|
2251
|
-
<text>Pom152p</text>
|
|
2252
|
-
</annotation>
|
|
2253
|
-
<annotation id="G104">
|
|
2254
|
-
<infon key="type">Gene</infon>
|
|
2255
|
-
<location offset="17884" length="10"/>
|
|
2256
|
-
<text>Ndc1p-ProA</text>
|
|
2257
|
-
</annotation>
|
|
2258
|
-
<annotation id="TEAM_277_40">
|
|
2259
|
-
<infon key="type">Gene</infon>
|
|
2260
|
-
<infon key="GeneID">203327</infon>
|
|
2261
|
-
<location offset="16868" length="3"/>
|
|
2262
|
-
<text>NEs</text>
|
|
2263
|
-
</annotation>
|
|
2264
|
-
<annotation id="TEAM_277_41">
|
|
2265
|
-
<infon key="type">Gene</infon>
|
|
2266
|
-
<infon key="GeneID">4415</infon>
|
|
2267
|
-
<location offset="17493" length="6"/>
|
|
2268
|
-
<text>Nup57p</text>
|
|
2269
|
-
</annotation>
|
|
2270
1371
|
</passage>
|
|
2271
1372
|
<passage>
|
|
2272
1373
|
<infon key="type">title_2</infon>
|
|
@@ -2278,19 +1379,6 @@
|
|
|
2278
1379
|
<location offset="17937" length="5"/>
|
|
2279
1380
|
<text>Ndc1p</text>
|
|
2280
1381
|
</annotation>
|
|
2281
|
-
<annotation id="G105">
|
|
2282
|
-
<infon key="score">0.73</infon>
|
|
2283
|
-
<infon key="GeneID">854977</infon>
|
|
2284
|
-
<infon key="type">Gene</infon>
|
|
2285
|
-
<location offset="17937" length="5"/>
|
|
2286
|
-
<text>Ndc1p</text>
|
|
2287
|
-
</annotation>
|
|
2288
|
-
<annotation id="TEAM_277_42">
|
|
2289
|
-
<infon key="type">Gene</infon>
|
|
2290
|
-
<infon key="GeneID">10277</infon>
|
|
2291
|
-
<location offset="17961" length="4"/>
|
|
2292
|
-
<text>SPBs</text>
|
|
2293
|
-
</annotation>
|
|
2294
1382
|
</passage>
|
|
2295
1383
|
<passage>
|
|
2296
1384
|
<infon key="type">paragraph</infon>
|
|
@@ -2314,40 +1402,6 @@
|
|
|
2314
1402
|
<location offset="17966" length="108"/>
|
|
2315
1403
|
<text>Because NDC1 function is required for proper SPB duplication, we tested whether Ndc1p also localizes to SPBs</text>
|
|
2316
1404
|
</annotation>
|
|
2317
|
-
<annotation id="G106">
|
|
2318
|
-
<infon key="score">0.70</infon>
|
|
2319
|
-
<infon key="GeneID">335236</infon>
|
|
2320
|
-
<infon key="type">Gene</infon>
|
|
2321
|
-
<location offset="17974" length="4"/>
|
|
2322
|
-
<text>NDC1</text>
|
|
2323
|
-
</annotation>
|
|
2324
|
-
<annotation id="G107">
|
|
2325
|
-
<infon key="score">0.73</infon>
|
|
2326
|
-
<infon key="GeneID">854977</infon>
|
|
2327
|
-
<infon key="type">Gene</infon>
|
|
2328
|
-
<location offset="18046" length="5"/>
|
|
2329
|
-
<text>Ndc1p</text>
|
|
2330
|
-
</annotation>
|
|
2331
|
-
<annotation id="G108">
|
|
2332
|
-
<infon key="type">Gene</infon>
|
|
2333
|
-
<location offset="18378" length="10"/>
|
|
2334
|
-
<text>Ndc1p-ProA</text>
|
|
2335
|
-
</annotation>
|
|
2336
|
-
<annotation id="G109">
|
|
2337
|
-
<infon key="type">Gene</infon>
|
|
2338
|
-
<location offset="18399" length="9"/>
|
|
2339
|
-
<text>Kilmartin</text>
|
|
2340
|
-
</annotation>
|
|
2341
|
-
<annotation id="G110">
|
|
2342
|
-
<infon key="type">Gene</infon>
|
|
2343
|
-
<location offset="18467" length="10"/>
|
|
2344
|
-
<text>Ndc1p-ProA</text>
|
|
2345
|
-
</annotation>
|
|
2346
|
-
<annotation id="TEAM_277_43">
|
|
2347
|
-
<infon key="type">Gene</infon>
|
|
2348
|
-
<location offset="17974" length="4"/>
|
|
2349
|
-
<text>NDC1</text>
|
|
2350
|
-
</annotation>
|
|
2351
1405
|
</passage>
|
|
2352
1406
|
<passage>
|
|
2353
1407
|
<infon key="type">paragraph</infon>
|
|
@@ -2411,174 +1465,40 @@
|
|
|
2411
1465
|
<location offset="19558" length="6"/>
|
|
2412
1466
|
<text>Nic96p</text>
|
|
2413
1467
|
</annotation>
|
|
2414
|
-
|
|
2415
|
-
|
|
2416
|
-
|
|
1468
|
+
</passage>
|
|
1469
|
+
<passage>
|
|
1470
|
+
<infon key="type">paragraph</infon>
|
|
1471
|
+
<offset>19836</offset>
|
|
1472
|
+
<text>We were able to distinguish between NPC and SPB signals using this technology. We observed a distinct spot of Ndc1p-ProA staining that colocalized with Spc42p-GFP signal in 91% of the SPBs examined (n = 63) (Fig. 3 A). In contrast, Nic96p-ProA staining did not exhibit colocalization with Spc42p-GFP signal in 82% of the SPBs examined (n = 51) (Fig. 3 B). Both Ndc1p and Nic96p contained the ProA epitope tag, allowing us to use the same antibodies to detect their localization. Additionally, the localization of Spc42p-GFP was visualized using GFP autofluorescence and therefore did not rely on potentially cross-reacting antibodies. </text>
|
|
1473
|
+
<annotation id="C278">
|
|
1474
|
+
<infon key="NCBI Gene">854977</infon>
|
|
2417
1475
|
<infon key="type">Gene</infon>
|
|
2418
|
-
<location offset="
|
|
1476
|
+
<location offset="20201" length="5"/>
|
|
2419
1477
|
<text>Ndc1p</text>
|
|
2420
1478
|
</annotation>
|
|
2421
|
-
<annotation id="
|
|
2422
|
-
<infon key="
|
|
2423
|
-
<infon key="GeneID">853824</infon>
|
|
1479
|
+
<annotation id="C279">
|
|
1480
|
+
<infon key="NCBI Gene">850552</infon>
|
|
2424
1481
|
<infon key="type">Gene</infon>
|
|
2425
|
-
<location offset="
|
|
2426
|
-
<text>
|
|
1482
|
+
<location offset="20211" length="6"/>
|
|
1483
|
+
<text>Nic96p</text>
|
|
2427
1484
|
</annotation>
|
|
2428
|
-
<annotation id="
|
|
2429
|
-
<infon key="
|
|
2430
|
-
<infon key="GeneID">853824</infon>
|
|
1485
|
+
<annotation id="C280">
|
|
1486
|
+
<infon key="NCBI Gene">854977</infon>
|
|
2431
1487
|
<infon key="type">Gene</infon>
|
|
2432
|
-
<location offset="
|
|
2433
|
-
<text>
|
|
1488
|
+
<location offset="20388" length="3"/>
|
|
1489
|
+
<text>GFP</text>
|
|
2434
1490
|
</annotation>
|
|
2435
|
-
<annotation id="
|
|
2436
|
-
<infon key="
|
|
2437
|
-
<
|
|
2438
|
-
<
|
|
2439
|
-
|
|
2440
|
-
<annotation id="G115">
|
|
2441
|
-
<infon key="type">Gene</infon>
|
|
2442
|
-
<location offset="19340" length="9"/>
|
|
2443
|
-
<text>Kilmartin</text>
|
|
2444
|
-
</annotation>
|
|
2445
|
-
<annotation id="G116">
|
|
2446
|
-
<infon key="score">0.73</infon>
|
|
2447
|
-
<infon key="GeneID">853824</infon>
|
|
2448
|
-
<infon key="type">Gene</infon>
|
|
2449
|
-
<location offset="19446" length="9"/>
|
|
2450
|
-
<text>SPC42-GFP</text>
|
|
2451
|
-
</annotation>
|
|
2452
|
-
<annotation id="G117">
|
|
2453
|
-
<infon key="score">0.73</infon>
|
|
2454
|
-
<infon key="GeneID">850552</infon>
|
|
2455
|
-
<infon key="type">Gene</infon>
|
|
2456
|
-
<location offset="19558" length="6"/>
|
|
2457
|
-
<text>Nic96p</text>
|
|
2458
|
-
</annotation>
|
|
2459
|
-
<annotation id="TEAM_277_44">
|
|
2460
|
-
<infon key="type">Gene</infon>
|
|
2461
|
-
<infon key="GeneID">10855</infon>
|
|
2462
|
-
<location offset="19177" length="5"/>
|
|
2463
|
-
<text>SPC42</text>
|
|
2464
|
-
</annotation>
|
|
2465
|
-
<annotation id="TEAM_277_45">
|
|
2466
|
-
<infon key="type">Gene</infon>
|
|
2467
|
-
<infon key="GeneID">10277</infon>
|
|
2468
|
-
<location offset="19691" length="4"/>
|
|
2469
|
-
<text>SPBs</text>
|
|
2470
|
-
</annotation>
|
|
2471
|
-
<annotation id="3">
|
|
2472
|
-
<infon key="type">ExperimentalMethod</infon>
|
|
2473
|
-
<infon key="PSIMI">0416</infon>
|
|
2474
|
-
<location offset="18781" length="311"/>
|
|
2475
|
-
<text>Therefore, we used whole cell indirect IF deconvolution microscopy to test whether any of the distinct spots of Ndc1p staining colocalize with signal from a known SPB component. This high-resolution fluorescence microscopy technique could potentially resolve SPB- and NPC-associated signals in the yeast NE.</text>
|
|
2476
|
-
</annotation>
|
|
2477
|
-
</passage>
|
|
2478
|
-
<passage>
|
|
2479
|
-
<infon key="type">paragraph</infon>
|
|
2480
|
-
<offset>19836</offset>
|
|
2481
|
-
<text>We were able to distinguish between NPC and SPB signals using this technology. We observed a distinct spot of Ndc1p-ProA staining that colocalized with Spc42p-GFP signal in 91% of the SPBs examined (n = 63) (Fig. 3 A). In contrast, Nic96p-ProA staining did not exhibit colocalization with Spc42p-GFP signal in 82% of the SPBs examined (n = 51) (Fig. 3 B). Both Ndc1p and Nic96p contained the ProA epitope tag, allowing us to use the same antibodies to detect their localization. Additionally, the localization of Spc42p-GFP was visualized using GFP autofluorescence and therefore did not rely on potentially cross-reacting antibodies. </text>
|
|
2482
|
-
<annotation id="C278">
|
|
2483
|
-
<infon key="NCBI Gene">854977</infon>
|
|
2484
|
-
<infon key="type">Gene</infon>
|
|
2485
|
-
<location offset="20201" length="5"/>
|
|
2486
|
-
<text>Ndc1p</text>
|
|
2487
|
-
</annotation>
|
|
2488
|
-
<annotation id="C279">
|
|
2489
|
-
<infon key="NCBI Gene">850552</infon>
|
|
2490
|
-
<infon key="type">Gene</infon>
|
|
2491
|
-
<location offset="20211" length="6"/>
|
|
2492
|
-
<text>Nic96p</text>
|
|
2493
|
-
</annotation>
|
|
2494
|
-
<annotation id="C280">
|
|
2495
|
-
<infon key="NCBI Gene">854977</infon>
|
|
2496
|
-
<infon key="type">Gene</infon>
|
|
2497
|
-
<location offset="20388" length="3"/>
|
|
2498
|
-
<text>GFP</text>
|
|
2499
|
-
</annotation>
|
|
2500
|
-
<annotation id="E8">
|
|
2501
|
-
<infon key="score">0.080469183</infon>
|
|
2502
|
-
<infon key="type">PPImention</infon>
|
|
2503
|
-
<location offset="20196" length="123"/>
|
|
2504
|
-
<text>Both Ndc1p and Nic96p contained the ProA epitope tag, allowing us to use the same antibodies to detect their localization</text>
|
|
2505
|
-
</annotation>
|
|
2506
|
-
<annotation id="G118">
|
|
2507
|
-
<infon key="type">Gene</infon>
|
|
2508
|
-
<location offset="19947" length="10"/>
|
|
2509
|
-
<text>Ndc1p-ProA</text>
|
|
2510
|
-
</annotation>
|
|
2511
|
-
<annotation id="G119">
|
|
2512
|
-
<infon key="score">0.73</infon>
|
|
2513
|
-
<infon key="GeneID">853824</infon>
|
|
2514
|
-
<infon key="type">Gene</infon>
|
|
2515
|
-
<location offset="19989" length="10"/>
|
|
2516
|
-
<text>Spc42p-GFP</text>
|
|
2517
|
-
</annotation>
|
|
2518
|
-
<annotation id="G120">
|
|
2519
|
-
<infon key="type">Gene</infon>
|
|
2520
|
-
<location offset="20071" length="11"/>
|
|
2521
|
-
<text>Nic96p-ProA</text>
|
|
2522
|
-
</annotation>
|
|
2523
|
-
<annotation id="G121">
|
|
2524
|
-
<infon key="score">0.73</infon>
|
|
2525
|
-
<infon key="GeneID">853824</infon>
|
|
2526
|
-
<infon key="type">Gene</infon>
|
|
2527
|
-
<location offset="20128" length="10"/>
|
|
2528
|
-
<text>Spc42p-GFP</text>
|
|
2529
|
-
</annotation>
|
|
2530
|
-
<annotation id="G122">
|
|
2531
|
-
<infon key="score">0.73</infon>
|
|
2532
|
-
<infon key="GeneID">854977</infon>
|
|
2533
|
-
<infon key="type">Gene</infon>
|
|
2534
|
-
<location offset="20201" length="5"/>
|
|
2535
|
-
<text>Ndc1p</text>
|
|
2536
|
-
</annotation>
|
|
2537
|
-
<annotation id="G123">
|
|
2538
|
-
<infon key="score">0.73</infon>
|
|
2539
|
-
<infon key="GeneID">850552</infon>
|
|
2540
|
-
<infon key="type">Gene</infon>
|
|
2541
|
-
<location offset="20211" length="6"/>
|
|
2542
|
-
<text>Nic96p</text>
|
|
2543
|
-
</annotation>
|
|
2544
|
-
<annotation id="G124">
|
|
2545
|
-
<infon key="score">0.70</infon>
|
|
2546
|
-
<infon key="GeneID">7502638</infon>
|
|
2547
|
-
<infon key="type">Gene</infon>
|
|
2548
|
-
<location offset="20233" length="12"/>
|
|
2549
|
-
<text>ProA epitope</text>
|
|
2550
|
-
</annotation>
|
|
2551
|
-
<annotation id="G125">
|
|
2552
|
-
<infon key="score">0.73</infon>
|
|
2553
|
-
<infon key="GeneID">853824</infon>
|
|
2554
|
-
<infon key="type">Gene</infon>
|
|
2555
|
-
<location offset="20356" length="10"/>
|
|
2556
|
-
<text>Spc42p-GFP</text>
|
|
1491
|
+
<annotation id="E8">
|
|
1492
|
+
<infon key="score">0.080469183</infon>
|
|
1493
|
+
<infon key="type">PPImention</infon>
|
|
1494
|
+
<location offset="20196" length="123"/>
|
|
1495
|
+
<text>Both Ndc1p and Nic96p contained the ProA epitope tag, allowing us to use the same antibodies to detect their localization</text>
|
|
2557
1496
|
</annotation>
|
|
2558
1497
|
</passage>
|
|
2559
1498
|
<passage>
|
|
2560
1499
|
<infon key="type">paragraph</infon>
|
|
2561
1500
|
<offset>20480</offset>
|
|
2562
1501
|
<text>As described earlier, when examining the colocalization of Ndc1p-ProA with Nup49p-GFP in a NPC clustering mutant strain, we occasionally observed either one or two distinct spots of Ndc1p-ProA staining that did not overlap with Nup49p-GFP signal (Fig. 3 C). It is likely that these additional spots of Ndc1p-ProA staining correspond to SPB localization. Previous work demonstrated that the SPB often localizes within NPC clusters (Heath et al., 1995), explaining why we observed extra spots of Ndc1p-ProA staining only occasionally.</text>
|
|
2563
|
-
<annotation id="G126">
|
|
2564
|
-
<infon key="score">0.73</infon>
|
|
2565
|
-
<infon key="GeneID">852703</infon>
|
|
2566
|
-
<infon key="type">Gene</infon>
|
|
2567
|
-
<location offset="20556" length="10"/>
|
|
2568
|
-
<text>Nup49p-GFP</text>
|
|
2569
|
-
</annotation>
|
|
2570
|
-
<annotation id="G127">
|
|
2571
|
-
<infon key="type">Gene</infon>
|
|
2572
|
-
<location offset="20665" length="10"/>
|
|
2573
|
-
<text>Ndc1p-ProA</text>
|
|
2574
|
-
</annotation>
|
|
2575
|
-
<annotation id="G128">
|
|
2576
|
-
<infon key="score">0.73</infon>
|
|
2577
|
-
<infon key="GeneID">852703</infon>
|
|
2578
|
-
<infon key="type">Gene</infon>
|
|
2579
|
-
<location offset="20712" length="10"/>
|
|
2580
|
-
<text>Nup49p-GFP</text>
|
|
2581
|
-
</annotation>
|
|
2582
1502
|
</passage>
|
|
2583
1503
|
<passage>
|
|
2584
1504
|
<infon key="type">paragraph</infon>
|
|
@@ -2590,74 +1510,11 @@
|
|
|
2590
1510
|
<location offset="21431" length="5"/>
|
|
2591
1511
|
<text>yeast</text>
|
|
2592
1512
|
</annotation>
|
|
2593
|
-
<annotation id="G129">
|
|
2594
|
-
<infon key="score">0.73</infon>
|
|
2595
|
-
<infon key="GeneID">854977</infon>
|
|
2596
|
-
<infon key="type">Gene</infon>
|
|
2597
|
-
<location offset="21062" length="9"/>
|
|
2598
|
-
<text>Ndc1p-GFP</text>
|
|
2599
|
-
</annotation>
|
|
2600
|
-
<annotation id="G130">
|
|
2601
|
-
<infon key="score">0.73</infon>
|
|
2602
|
-
<infon key="GeneID">854977</infon>
|
|
2603
|
-
<infon key="type">Gene</infon>
|
|
2604
|
-
<location offset="21165" length="9"/>
|
|
2605
|
-
<text>Ndc1p-GFP</text>
|
|
2606
|
-
</annotation>
|
|
2607
|
-
<annotation id="G131">
|
|
2608
|
-
<infon key="score">0.73</infon>
|
|
2609
|
-
<infon key="GeneID">854977</infon>
|
|
2610
|
-
<infon key="type">Gene</infon>
|
|
2611
|
-
<location offset="21239" length="9"/>
|
|
2612
|
-
<text>Ndc1p-GFP</text>
|
|
2613
|
-
</annotation>
|
|
2614
|
-
<annotation id="G132">
|
|
2615
|
-
<infon key="score">0.73</infon>
|
|
2616
|
-
<infon key="GeneID">854977</infon>
|
|
2617
|
-
<infon key="type">Gene</infon>
|
|
2618
|
-
<location offset="21583" length="9"/>
|
|
2619
|
-
<text>Ndc1p-GFP</text>
|
|
2620
|
-
</annotation>
|
|
2621
|
-
<annotation id="G133">
|
|
2622
|
-
<infon key="score">0.73</infon>
|
|
2623
|
-
<infon key="GeneID">854977</infon>
|
|
2624
|
-
<infon key="type">Gene</infon>
|
|
2625
|
-
<location offset="21975" length="9"/>
|
|
2626
|
-
<text>Ndc1p-GFP</text>
|
|
2627
|
-
</annotation>
|
|
2628
|
-
<annotation id="TEAM_277_46">
|
|
2629
|
-
<infon key="type">Gene</infon>
|
|
2630
|
-
<location offset="21239" length="9"/>
|
|
2631
|
-
<text>Ndc1p-GFP</text>
|
|
2632
|
-
</annotation>
|
|
2633
|
-
<annotation id="TEAM_277_47">
|
|
2634
|
-
<infon key="type">Gene</infon>
|
|
2635
|
-
<location offset="21583" length="9"/>
|
|
2636
|
-
<text>Ndc1p-GFP</text>
|
|
2637
|
-
</annotation>
|
|
2638
1513
|
</passage>
|
|
2639
1514
|
<passage>
|
|
2640
1515
|
<infon key="type">title_2</infon>
|
|
2641
1516
|
<offset>22047</offset>
|
|
2642
1517
|
<text>Immunoelectron Microscopy Demonstrates that Ndc1p-GFP Localizes to the Membrane-associated Regions of NPCs and SPBs</text>
|
|
2643
|
-
<annotation id="G134">
|
|
2644
|
-
<infon key="score">0.73</infon>
|
|
2645
|
-
<infon key="GeneID">854977</infon>
|
|
2646
|
-
<infon key="type">Gene</infon>
|
|
2647
|
-
<location offset="22091" length="9"/>
|
|
2648
|
-
<text>Ndc1p-GFP</text>
|
|
2649
|
-
</annotation>
|
|
2650
|
-
<annotation id="TEAM_277_48">
|
|
2651
|
-
<infon key="type">Gene</infon>
|
|
2652
|
-
<location offset="22091" length="9"/>
|
|
2653
|
-
<text>Ndc1p-GFP</text>
|
|
2654
|
-
</annotation>
|
|
2655
|
-
<annotation id="TEAM_277_49">
|
|
2656
|
-
<infon key="type">Gene</infon>
|
|
2657
|
-
<infon key="GeneID">10277</infon>
|
|
2658
|
-
<location offset="22159" length="4"/>
|
|
2659
|
-
<text>SPBs</text>
|
|
2660
|
-
</annotation>
|
|
2661
1518
|
</passage>
|
|
2662
1519
|
<passage>
|
|
2663
1520
|
<infon key="type">paragraph</infon>
|
|
@@ -2687,42 +1544,11 @@
|
|
|
2687
1544
|
<location offset="22489" length="3"/>
|
|
2688
1545
|
<text>GFP</text>
|
|
2689
1546
|
</annotation>
|
|
2690
|
-
<annotation id="G135">
|
|
2691
|
-
<infon key="score">0.73</infon>
|
|
2692
|
-
<infon key="GeneID">854977</infon>
|
|
2693
|
-
<infon key="type">Gene</infon>
|
|
2694
|
-
<location offset="22250" length="5"/>
|
|
2695
|
-
<text>Ndc1p</text>
|
|
2696
|
-
</annotation>
|
|
2697
|
-
<annotation id="G136">
|
|
2698
|
-
<infon key="score">0.73</infon>
|
|
2699
|
-
<infon key="GeneID">854977</infon>
|
|
2700
|
-
<infon key="type">Gene</infon>
|
|
2701
|
-
<location offset="22374" length="9"/>
|
|
2702
|
-
<text>Ndc1p-GFP</text>
|
|
2703
|
-
</annotation>
|
|
2704
|
-
<annotation id="G137">
|
|
2705
|
-
<infon key="score">0.73</infon>
|
|
2706
|
-
<infon key="GeneID">854977</infon>
|
|
2707
|
-
<infon key="type">Gene</infon>
|
|
2708
|
-
<location offset="22597" length="9"/>
|
|
2709
|
-
<text>Ndc1p-GFP</text>
|
|
2710
|
-
</annotation>
|
|
2711
|
-
<annotation id="G138">
|
|
2712
|
-
<infon key="type">Gene</infon>
|
|
2713
|
-
<location offset="22903" length="10"/>
|
|
2714
|
-
<text>Ndc1p-ProA</text>
|
|
2715
|
-
</annotation>
|
|
2716
1547
|
</passage>
|
|
2717
1548
|
<passage>
|
|
2718
1549
|
<infon key="type">title_2</infon>
|
|
2719
1550
|
<offset>23036</offset>
|
|
2720
1551
|
<text>ndc1-1 Strains Do Not Exhibit Defects in NPC Functions</text>
|
|
2721
|
-
<annotation id="G139">
|
|
2722
|
-
<infon key="type">Gene</infon>
|
|
2723
|
-
<location offset="23036" length="14"/>
|
|
2724
|
-
<text>ndc1-1 Strains</text>
|
|
2725
|
-
</annotation>
|
|
2726
1552
|
</passage>
|
|
2727
1553
|
<passage>
|
|
2728
1554
|
<infon key="type">paragraph</infon>
|
|
@@ -2752,55 +1578,6 @@
|
|
|
2752
1578
|
<location offset="23613" length="4"/>
|
|
2753
1579
|
<text>NDC1</text>
|
|
2754
1580
|
</annotation>
|
|
2755
|
-
<annotation id="G140">
|
|
2756
|
-
<infon key="score">0.73</infon>
|
|
2757
|
-
<infon key="GeneID">854977</infon>
|
|
2758
|
-
<infon key="type">Gene</infon>
|
|
2759
|
-
<location offset="23102" length="5"/>
|
|
2760
|
-
<text>Ndc1p</text>
|
|
2761
|
-
</annotation>
|
|
2762
|
-
<annotation id="G141">
|
|
2763
|
-
<infon key="score">0.73</infon>
|
|
2764
|
-
<infon key="GeneID">854977</infon>
|
|
2765
|
-
<infon key="type">Gene</infon>
|
|
2766
|
-
<location offset="23197" length="5"/>
|
|
2767
|
-
<text>Ndc1p</text>
|
|
2768
|
-
</annotation>
|
|
2769
|
-
<annotation id="G142">
|
|
2770
|
-
<infon key="score">0.73</infon>
|
|
2771
|
-
<infon key="GeneID">854977</infon>
|
|
2772
|
-
<infon key="type">Gene</infon>
|
|
2773
|
-
<location offset="23277" length="5"/>
|
|
2774
|
-
<text>Ndc1p</text>
|
|
2775
|
-
</annotation>
|
|
2776
|
-
<annotation id="G143">
|
|
2777
|
-
<infon key="type">Gene</infon>
|
|
2778
|
-
<location offset="23421" length="6"/>
|
|
2779
|
-
<text>ndc1-1</text>
|
|
2780
|
-
</annotation>
|
|
2781
|
-
<annotation id="G144">
|
|
2782
|
-
<infon key="score">0.70</infon>
|
|
2783
|
-
<infon key="GeneID">335236</infon>
|
|
2784
|
-
<infon key="type">Gene</infon>
|
|
2785
|
-
<location offset="23613" length="4"/>
|
|
2786
|
-
<text>NDC1</text>
|
|
2787
|
-
</annotation>
|
|
2788
|
-
<annotation id="G145">
|
|
2789
|
-
<infon key="type">Gene</infon>
|
|
2790
|
-
<location offset="23764" length="6"/>
|
|
2791
|
-
<text>ndc1-1</text>
|
|
2792
|
-
</annotation>
|
|
2793
|
-
<annotation id="TEAM_277_50">
|
|
2794
|
-
<infon key="type">Gene</infon>
|
|
2795
|
-
<infon key="GeneID">10277</infon>
|
|
2796
|
-
<location offset="23111" length="4"/>
|
|
2797
|
-
<text>SPBs</text>
|
|
2798
|
-
</annotation>
|
|
2799
|
-
<annotation id="TEAM_277_51">
|
|
2800
|
-
<infon key="type">Gene</infon>
|
|
2801
|
-
<location offset="23613" length="4"/>
|
|
2802
|
-
<text>NDC1</text>
|
|
2803
|
-
</annotation>
|
|
2804
1581
|
</passage>
|
|
2805
1582
|
<passage>
|
|
2806
1583
|
<infon key="type">paragraph</infon>
|
|
@@ -2847,97 +1624,6 @@
|
|
|
2847
1624
|
<location offset="25042" length="6"/>
|
|
2848
1625
|
<text>Nic96p</text>
|
|
2849
1626
|
</annotation>
|
|
2850
|
-
<annotation id="G146">
|
|
2851
|
-
<infon key="type">Gene</infon>
|
|
2852
|
-
<location offset="23854" length="6"/>
|
|
2853
|
-
<text>ndc1-1</text>
|
|
2854
|
-
</annotation>
|
|
2855
|
-
<annotation id="G147">
|
|
2856
|
-
<infon key="type">Gene</infon>
|
|
2857
|
-
<location offset="23902" length="13"/>
|
|
2858
|
-
<text>histone H2B1</text>
|
|
2859
|
-
</annotation>
|
|
2860
|
-
<annotation id="G148">
|
|
2861
|
-
<infon key="score">0.73</infon>
|
|
2862
|
-
<infon key="GeneID">7011691</infon>
|
|
2863
|
-
<infon key="type">Gene</infon>
|
|
2864
|
-
<location offset="23964" length="3"/>
|
|
2865
|
-
<text>GFP</text>
|
|
2866
|
-
</annotation>
|
|
2867
|
-
<annotation id="G149">
|
|
2868
|
-
<infon key="type">Gene</infon>
|
|
2869
|
-
<location offset="24057" length="9"/>
|
|
2870
|
-
<text>H2B1p-GFP</text>
|
|
2871
|
-
</annotation>
|
|
2872
|
-
<annotation id="G150">
|
|
2873
|
-
<infon key="type">Gene</infon>
|
|
2874
|
-
<location offset="24371" length="27"/>
|
|
2875
|
-
<text>yeast ribosomal protein L29</text>
|
|
2876
|
-
</annotation>
|
|
2877
|
-
<annotation id="G151">
|
|
2878
|
-
<infon key="score">0.70</infon>
|
|
2879
|
-
<infon key="GeneID">12091</infon>
|
|
2880
|
-
<infon key="type">Gene</infon>
|
|
2881
|
-
<location offset="24408" length="18"/>
|
|
2882
|
-
<text>beta-galactosidase</text>
|
|
2883
|
-
</annotation>
|
|
2884
|
-
<annotation id="G152">
|
|
2885
|
-
<infon key="type">Gene</infon>
|
|
2886
|
-
<location offset="24477" length="14"/>
|
|
2887
|
-
<text>GAL10 promoter</text>
|
|
2888
|
-
</annotation>
|
|
2889
|
-
<annotation id="G153">
|
|
2890
|
-
<infon key="score">0.70</infon>
|
|
2891
|
-
<infon key="GeneID">12091</infon>
|
|
2892
|
-
<infon key="type">Gene</infon>
|
|
2893
|
-
<location offset="24592" length="18"/>
|
|
2894
|
-
<text>beta-galactosidase</text>
|
|
2895
|
-
</annotation>
|
|
2896
|
-
<annotation id="G154">
|
|
2897
|
-
<infon key="type">Gene</infon>
|
|
2898
|
-
<location offset="24642" length="6"/>
|
|
2899
|
-
<text>ndc1-1</text>
|
|
2900
|
-
</annotation>
|
|
2901
|
-
<annotation id="G155">
|
|
2902
|
-
<infon key="type">Gene</infon>
|
|
2903
|
-
<location offset="24760" length="6"/>
|
|
2904
|
-
<text>ndc1-1</text>
|
|
2905
|
-
</annotation>
|
|
2906
|
-
<annotation id="G156">
|
|
2907
|
-
<infon key="type">Gene</infon>
|
|
2908
|
-
<location offset="25021" length="6"/>
|
|
2909
|
-
<text>ndc1-1</text>
|
|
2910
|
-
</annotation>
|
|
2911
|
-
<annotation id="G157">
|
|
2912
|
-
<infon key="score">0.73</infon>
|
|
2913
|
-
<infon key="GeneID">850552</infon>
|
|
2914
|
-
<infon key="type">Gene</infon>
|
|
2915
|
-
<location offset="25042" length="6"/>
|
|
2916
|
-
<text>Nic96p</text>
|
|
2917
|
-
</annotation>
|
|
2918
|
-
<annotation id="TEAM_277_52">
|
|
2919
|
-
<infon key="type">Gene</infon>
|
|
2920
|
-
<location offset="24057" length="9"/>
|
|
2921
|
-
<text>H2B1p-GFP</text>
|
|
2922
|
-
</annotation>
|
|
2923
|
-
<annotation id="TEAM_277_53">
|
|
2924
|
-
<infon key="type">Gene</infon>
|
|
2925
|
-
<infon key="GeneID">1081679</infon>
|
|
2926
|
-
<location offset="24408" length="18"/>
|
|
2927
|
-
<text>beta-galactosidase</text>
|
|
2928
|
-
</annotation>
|
|
2929
|
-
<annotation id="TEAM_277_54">
|
|
2930
|
-
<infon key="type">Gene</infon>
|
|
2931
|
-
<infon key="GeneID">119197</infon>
|
|
2932
|
-
<location offset="24477" length="14"/>
|
|
2933
|
-
<text>GAL10 promoter</text>
|
|
2934
|
-
</annotation>
|
|
2935
|
-
<annotation id="TEAM_277_55">
|
|
2936
|
-
<infon key="type">Gene</infon>
|
|
2937
|
-
<infon key="GeneID">1081679</infon>
|
|
2938
|
-
<location offset="24592" length="18"/>
|
|
2939
|
-
<text>beta-galactosidase</text>
|
|
2940
|
-
</annotation>
|
|
2941
1627
|
</passage>
|
|
2942
1628
|
<passage>
|
|
2943
1629
|
<infon key="type">paragraph</infon>
|
|
@@ -2983,73 +1669,6 @@
|
|
|
2983
1669
|
<location offset="25808" length="5"/>
|
|
2984
1670
|
<text>yeast</text>
|
|
2985
1671
|
</annotation>
|
|
2986
|
-
<annotation id="G158">
|
|
2987
|
-
<infon key="type">Gene</infon>
|
|
2988
|
-
<location offset="25330" length="6"/>
|
|
2989
|
-
<text>ndc1-1</text>
|
|
2990
|
-
</annotation>
|
|
2991
|
-
<annotation id="G159">
|
|
2992
|
-
<infon key="score">0.73</infon>
|
|
2993
|
-
<infon key="GeneID">850552</infon>
|
|
2994
|
-
<infon key="type">Gene</infon>
|
|
2995
|
-
<location offset="25393" length="6"/>
|
|
2996
|
-
<text>Nic96p</text>
|
|
2997
|
-
</annotation>
|
|
2998
|
-
<annotation id="G160">
|
|
2999
|
-
<infon key="type">Gene</infon>
|
|
3000
|
-
<location offset="25422" length="6"/>
|
|
3001
|
-
<text>ndc1-1</text>
|
|
3002
|
-
</annotation>
|
|
3003
|
-
<annotation id="G161">
|
|
3004
|
-
<infon key="type">Gene</infon>
|
|
3005
|
-
<location offset="25450" length="6"/>
|
|
3006
|
-
<text>ndc1-1</text>
|
|
3007
|
-
</annotation>
|
|
3008
|
-
<annotation id="G162">
|
|
3009
|
-
<infon key="type">Gene</infon>
|
|
3010
|
-
<location offset="25513" length="10"/>
|
|
3011
|
-
<text>NIC96-ProA</text>
|
|
3012
|
-
</annotation>
|
|
3013
|
-
<annotation id="G163">
|
|
3014
|
-
<infon key="score">0.70</infon>
|
|
3015
|
-
<infon key="GeneID">7502638</infon>
|
|
3016
|
-
<infon key="type">Gene</infon>
|
|
3017
|
-
<location offset="25597" length="4"/>
|
|
3018
|
-
<text>ProA</text>
|
|
3019
|
-
</annotation>
|
|
3020
|
-
<annotation id="G164">
|
|
3021
|
-
<infon key="type">Gene</infon>
|
|
3022
|
-
<location offset="25638" length="11"/>
|
|
3023
|
-
<text>Nic96p-ProA</text>
|
|
3024
|
-
</annotation>
|
|
3025
|
-
<annotation id="G165">
|
|
3026
|
-
<infon key="score">0.70</infon>
|
|
3027
|
-
<infon key="GeneID">850552</infon>
|
|
3028
|
-
<infon key="type">Gene</infon>
|
|
3029
|
-
<location offset="25734" length="5"/>
|
|
3030
|
-
<text>NIC96</text>
|
|
3031
|
-
</annotation>
|
|
3032
|
-
<annotation id="G166">
|
|
3033
|
-
<infon key="type">Gene</infon>
|
|
3034
|
-
<location offset="25881" length="10"/>
|
|
3035
|
-
<text>NIC96-ProA</text>
|
|
3036
|
-
</annotation>
|
|
3037
|
-
<annotation id="G167">
|
|
3038
|
-
<infon key="type">Gene</infon>
|
|
3039
|
-
<location offset="25923" length="6"/>
|
|
3040
|
-
<text>ndc1-1</text>
|
|
3041
|
-
</annotation>
|
|
3042
|
-
<annotation id="G168">
|
|
3043
|
-
<infon key="type">Gene</infon>
|
|
3044
|
-
<location offset="26151" length="11"/>
|
|
3045
|
-
<text>Nic96p-ProA</text>
|
|
3046
|
-
</annotation>
|
|
3047
|
-
<annotation id="TEAM_277_56">
|
|
3048
|
-
<infon key="type">Gene</infon>
|
|
3049
|
-
<infon key="GeneID">224119</infon>
|
|
3050
|
-
<location offset="25734" length="5"/>
|
|
3051
|
-
<text>NIC96</text>
|
|
3052
|
-
</annotation>
|
|
3053
1672
|
</passage>
|
|
3054
1673
|
<passage>
|
|
3055
1674
|
<infon key="type">paragraph</infon>
|
|
@@ -3067,54 +1686,11 @@
|
|
|
3067
1686
|
<location offset="27258" length="5"/>
|
|
3068
1687
|
<text>Wx340</text>
|
|
3069
1688
|
</annotation>
|
|
3070
|
-
<annotation id="G169">
|
|
3071
|
-
<infon key="type">Gene</infon>
|
|
3072
|
-
<location offset="26939" length="15"/>
|
|
3073
|
-
<text>tub2-403 allele</text>
|
|
3074
|
-
</annotation>
|
|
3075
|
-
<annotation id="G170">
|
|
3076
|
-
<infon key="score">0.58</infon>
|
|
3077
|
-
<infon key="GeneID">37888</infon>
|
|
3078
|
-
<infon key="type">Gene</infon>
|
|
3079
|
-
<location offset="26987" length="12"/>
|
|
3080
|
-
<text>beta-tubulin</text>
|
|
3081
|
-
</annotation>
|
|
3082
|
-
<annotation id="G171">
|
|
3083
|
-
<infon key="type">Gene</infon>
|
|
3084
|
-
<location offset="27053" length="10"/>
|
|
3085
|
-
<text>ndc1-1 SPB</text>
|
|
3086
|
-
</annotation>
|
|
3087
|
-
<annotation id="G172">
|
|
3088
|
-
<infon key="type">Gene</infon>
|
|
3089
|
-
<location offset="27128" length="6"/>
|
|
3090
|
-
<text>ndc1-1</text>
|
|
3091
|
-
</annotation>
|
|
3092
|
-
<annotation id="G173">
|
|
3093
|
-
<infon key="type">Gene</infon>
|
|
3094
|
-
<location offset="27235" length="15"/>
|
|
3095
|
-
<text>tub2-403 allele</text>
|
|
3096
|
-
</annotation>
|
|
3097
|
-
<annotation id="TEAM_277_57">
|
|
3098
|
-
<infon key="type">Gene</infon>
|
|
3099
|
-
<infon key="GeneID">130530</infon>
|
|
3100
|
-
<location offset="26987" length="12"/>
|
|
3101
|
-
<text>beta-tubulin</text>
|
|
3102
|
-
</annotation>
|
|
3103
1689
|
</passage>
|
|
3104
1690
|
<passage>
|
|
3105
1691
|
<infon key="type">paragraph</infon>
|
|
3106
1692
|
<offset>27344</offset>
|
|
3107
1693
|
<text>Samples were prepared as previously described (Winey et al., 1997) to directly count the number of NPCs in three nuclei from each strain after a shift to the nonpermissive temperature. Nuclei from the tub2-403 cells contained 162, 179, and 232 NPCs, and nuclei from the ndc1-1, tub2-403 double mutant cells contained 180, 193, and 209 NPCs. The maximum number of NPCs observed in wild-type cells occurs in early mitosis, with an average of 142 +- 16.4 NPCs (Winey et al., 1997). Our analysis demonstrated that both strains contained more NPCs than observed in wild-type cells at any point in the cell cycle, indicating that NPC assembly continues to occur when these cells are arrested.</text>
|
|
3108
|
-
<annotation id="G174">
|
|
3109
|
-
<infon key="type">Gene</infon>
|
|
3110
|
-
<location offset="27618" length="6"/>
|
|
3111
|
-
<text>ndc1-1</text>
|
|
3112
|
-
</annotation>
|
|
3113
|
-
<annotation id="G175">
|
|
3114
|
-
<infon key="type">Gene</infon>
|
|
3115
|
-
<location offset="27626" length="8"/>
|
|
3116
|
-
<text>tub2-403</text>
|
|
3117
|
-
</annotation>
|
|
3118
1694
|
</passage>
|
|
3119
1695
|
<passage>
|
|
3120
1696
|
<infon key="type">title_2</infon>
|
|
@@ -3131,16 +1707,6 @@
|
|
|
3131
1707
|
<location offset="28055" length="6"/>
|
|
3132
1708
|
<text>POM152</text>
|
|
3133
1709
|
</annotation>
|
|
3134
|
-
<annotation id="G176">
|
|
3135
|
-
<infon key="type">Gene</infon>
|
|
3136
|
-
<location offset="28077" length="10"/>
|
|
3137
|
-
<text>ndc1-1 SPB</text>
|
|
3138
|
-
</annotation>
|
|
3139
|
-
<annotation id="TEAM_277_58">
|
|
3140
|
-
<infon key="type">Gene</infon>
|
|
3141
|
-
<location offset="28055" length="6"/>
|
|
3142
|
-
<text>POM152</text>
|
|
3143
|
-
</annotation>
|
|
3144
1710
|
</passage>
|
|
3145
1711
|
<passage>
|
|
3146
1712
|
<infon key="type">paragraph</infon>
|
|
@@ -3213,64 +1779,6 @@
|
|
|
3213
1779
|
<location offset="28108" length="115"/>
|
|
3214
1780
|
<text>To define a potential functional role for Ndc1p in NPCs, we examined genetic interactions between NDC1 and POM152</text>
|
|
3215
1781
|
</annotation>
|
|
3216
|
-
<annotation id="G177">
|
|
3217
|
-
<infon key="score">0.73</infon>
|
|
3218
|
-
<infon key="GeneID">854977</infon>
|
|
3219
|
-
<infon key="type">Gene</infon>
|
|
3220
|
-
<location offset="28150" length="5"/>
|
|
3221
|
-
<text>Ndc1p</text>
|
|
3222
|
-
</annotation>
|
|
3223
|
-
<annotation id="G178">
|
|
3224
|
-
<infon key="score">0.70</infon>
|
|
3225
|
-
<infon key="GeneID">335236</infon>
|
|
3226
|
-
<infon key="type">Gene</infon>
|
|
3227
|
-
<location offset="28207" length="4"/>
|
|
3228
|
-
<text>NDC1</text>
|
|
3229
|
-
</annotation>
|
|
3230
|
-
<annotation id="G179">
|
|
3231
|
-
<infon key="score">0.70</infon>
|
|
3232
|
-
<infon key="GeneID">855159</infon>
|
|
3233
|
-
<infon key="type">Gene</infon>
|
|
3234
|
-
<location offset="28217" length="6"/>
|
|
3235
|
-
<text>POM152</text>
|
|
3236
|
-
</annotation>
|
|
3237
|
-
<annotation id="G180">
|
|
3238
|
-
<infon key="score">0.70</infon>
|
|
3239
|
-
<infon key="GeneID">855159</infon>
|
|
3240
|
-
<infon key="type">Gene</infon>
|
|
3241
|
-
<location offset="28400" length="6"/>
|
|
3242
|
-
<text>POM152</text>
|
|
3243
|
-
</annotation>
|
|
3244
|
-
<annotation id="G181">
|
|
3245
|
-
<infon key="type">Gene</infon>
|
|
3246
|
-
<location offset="28539" length="6"/>
|
|
3247
|
-
<text>ndc1-1</text>
|
|
3248
|
-
</annotation>
|
|
3249
|
-
<annotation id="G182">
|
|
3250
|
-
<infon key="type">Gene</infon>
|
|
3251
|
-
<location offset="28555" length="18"/>
|
|
3252
|
-
<text>pom152 null allele</text>
|
|
3253
|
-
</annotation>
|
|
3254
|
-
<annotation id="TEAM_277_59">
|
|
3255
|
-
<infon key="type">Gene</infon>
|
|
3256
|
-
<location offset="28207" length="4"/>
|
|
3257
|
-
<text>NDC1</text>
|
|
3258
|
-
</annotation>
|
|
3259
|
-
<annotation id="TEAM_277_60">
|
|
3260
|
-
<infon key="type">Gene</infon>
|
|
3261
|
-
<location offset="28217" length="6"/>
|
|
3262
|
-
<text>POM152</text>
|
|
3263
|
-
</annotation>
|
|
3264
|
-
<annotation id="TEAM_277_61">
|
|
3265
|
-
<infon key="type">Gene</infon>
|
|
3266
|
-
<location offset="28260" length="6"/>
|
|
3267
|
-
<text>POM152</text>
|
|
3268
|
-
</annotation>
|
|
3269
|
-
<annotation id="TEAM_277_62">
|
|
3270
|
-
<infon key="type">Gene</infon>
|
|
3271
|
-
<location offset="28400" length="6"/>
|
|
3272
|
-
<text>POM152</text>
|
|
3273
|
-
</annotation>
|
|
3274
1782
|
</passage>
|
|
3275
1783
|
<passage>
|
|
3276
1784
|
<infon key="type">paragraph</infon>
|
|
@@ -3337,85 +1845,6 @@
|
|
|
3337
1845
|
<location offset="29270" length="4"/>
|
|
3338
1846
|
<text>ndc1</text>
|
|
3339
1847
|
</annotation>
|
|
3340
|
-
<annotation id="G183">
|
|
3341
|
-
<infon key="type">Gene</infon>
|
|
3342
|
-
<location offset="28687" length="18"/>
|
|
3343
|
-
<text>pom152 null allele</text>
|
|
3344
|
-
</annotation>
|
|
3345
|
-
<annotation id="G184">
|
|
3346
|
-
<infon key="type">Gene</infon>
|
|
3347
|
-
<location offset="28721" length="6"/>
|
|
3348
|
-
<text>ndc1-1</text>
|
|
3349
|
-
</annotation>
|
|
3350
|
-
<annotation id="G185">
|
|
3351
|
-
<infon key="type">Gene</infon>
|
|
3352
|
-
<location offset="28754" length="6"/>
|
|
3353
|
-
<text>ndc1-1</text>
|
|
3354
|
-
</annotation>
|
|
3355
|
-
<annotation id="G186">
|
|
3356
|
-
<infon key="type">Gene</infon>
|
|
3357
|
-
<location offset="28785" length="18"/>
|
|
3358
|
-
<text>pom152 null allele</text>
|
|
3359
|
-
</annotation>
|
|
3360
|
-
<annotation id="G187">
|
|
3361
|
-
<infon key="type">Gene</infon>
|
|
3362
|
-
<location offset="28887" length="6"/>
|
|
3363
|
-
<text>ndc1-1</text>
|
|
3364
|
-
</annotation>
|
|
3365
|
-
<annotation id="G188">
|
|
3366
|
-
<infon key="score">0.70</infon>
|
|
3367
|
-
<infon key="GeneID">855159</infon>
|
|
3368
|
-
<infon key="type">Gene</infon>
|
|
3369
|
-
<location offset="28958" length="6"/>
|
|
3370
|
-
<text>POM152</text>
|
|
3371
|
-
</annotation>
|
|
3372
|
-
<annotation id="G189">
|
|
3373
|
-
<infon key="score">0.70</infon>
|
|
3374
|
-
<infon key="GeneID">855159</infon>
|
|
3375
|
-
<infon key="type">Gene</infon>
|
|
3376
|
-
<location offset="29004" length="6"/>
|
|
3377
|
-
<text>POM152</text>
|
|
3378
|
-
</annotation>
|
|
3379
|
-
<annotation id="G190">
|
|
3380
|
-
<infon key="type">Gene</infon>
|
|
3381
|
-
<location offset="29064" length="6"/>
|
|
3382
|
-
<text>ndc1-1</text>
|
|
3383
|
-
</annotation>
|
|
3384
|
-
<annotation id="G191">
|
|
3385
|
-
<infon key="type">Gene</infon>
|
|
3386
|
-
<location offset="29174" length="13"/>
|
|
3387
|
-
<text>ndc1-1 allele</text>
|
|
3388
|
-
</annotation>
|
|
3389
|
-
<annotation id="G192">
|
|
3390
|
-
<infon key="type">Gene</infon>
|
|
3391
|
-
<location offset="29201" length="18"/>
|
|
3392
|
-
<text>pom152 null allele</text>
|
|
3393
|
-
</annotation>
|
|
3394
|
-
<annotation id="G193">
|
|
3395
|
-
<infon key="type">Gene</infon>
|
|
3396
|
-
<location offset="29270" length="16"/>
|
|
3397
|
-
<text>ndc1 null allele</text>
|
|
3398
|
-
</annotation>
|
|
3399
|
-
<annotation id="TEAM_277_63">
|
|
3400
|
-
<infon key="type">Gene</infon>
|
|
3401
|
-
<location offset="28785" length="6"/>
|
|
3402
|
-
<text>pom152</text>
|
|
3403
|
-
</annotation>
|
|
3404
|
-
<annotation id="TEAM_277_64">
|
|
3405
|
-
<infon key="type">Gene</infon>
|
|
3406
|
-
<location offset="28958" length="6"/>
|
|
3407
|
-
<text>POM152</text>
|
|
3408
|
-
</annotation>
|
|
3409
|
-
<annotation id="TEAM_277_65">
|
|
3410
|
-
<infon key="type">Gene</infon>
|
|
3411
|
-
<location offset="29004" length="6"/>
|
|
3412
|
-
<text>POM152</text>
|
|
3413
|
-
</annotation>
|
|
3414
|
-
<annotation id="TEAM_277_66">
|
|
3415
|
-
<infon key="type">Gene</infon>
|
|
3416
|
-
<location offset="29270" length="4"/>
|
|
3417
|
-
<text>ndc1</text>
|
|
3418
|
-
</annotation>
|
|
3419
1848
|
</passage>
|
|
3420
1849
|
<passage>
|
|
3421
1850
|
<infon key="type">paragraph</infon>
|
|
@@ -3460,82 +1889,6 @@
|
|
|
3460
1889
|
<location offset="30295" length="6"/>
|
|
3461
1890
|
<text>pom152</text>
|
|
3462
1891
|
</annotation>
|
|
3463
|
-
<annotation id="G194">
|
|
3464
|
-
<infon key="score">0.70</infon>
|
|
3465
|
-
<infon key="GeneID">335236</infon>
|
|
3466
|
-
<infon key="type">Gene</infon>
|
|
3467
|
-
<location offset="29308" length="4"/>
|
|
3468
|
-
<text>NDC1</text>
|
|
3469
|
-
</annotation>
|
|
3470
|
-
<annotation id="G195">
|
|
3471
|
-
<infon key="score">0.70</infon>
|
|
3472
|
-
<infon key="GeneID">855159</infon>
|
|
3473
|
-
<infon key="type">Gene</infon>
|
|
3474
|
-
<location offset="29507" length="6"/>
|
|
3475
|
-
<text>POM152</text>
|
|
3476
|
-
</annotation>
|
|
3477
|
-
<annotation id="G196">
|
|
3478
|
-
<infon key="type">Gene</infon>
|
|
3479
|
-
<location offset="29530" length="10"/>
|
|
3480
|
-
<text>ndc1-1 SPB</text>
|
|
3481
|
-
</annotation>
|
|
3482
|
-
<annotation id="G197">
|
|
3483
|
-
<infon key="type">Gene</infon>
|
|
3484
|
-
<location offset="29784" length="6"/>
|
|
3485
|
-
<text>ndc1-1</text>
|
|
3486
|
-
</annotation>
|
|
3487
|
-
<annotation id="G198">
|
|
3488
|
-
<infon key="type">Gene</infon>
|
|
3489
|
-
<location offset="29939" length="6"/>
|
|
3490
|
-
<text>ndc1-1</text>
|
|
3491
|
-
</annotation>
|
|
3492
|
-
<annotation id="G199">
|
|
3493
|
-
<infon key="type">Gene</infon>
|
|
3494
|
-
<location offset="30123" length="6"/>
|
|
3495
|
-
<text>ndc1-1</text>
|
|
3496
|
-
</annotation>
|
|
3497
|
-
<annotation id="G200">
|
|
3498
|
-
<infon key="score">0.70</infon>
|
|
3499
|
-
<infon key="GeneID">855159</infon>
|
|
3500
|
-
<infon key="type">Gene</infon>
|
|
3501
|
-
<location offset="30131" length="6"/>
|
|
3502
|
-
<text>pom152</text>
|
|
3503
|
-
</annotation>
|
|
3504
|
-
<annotation id="G201">
|
|
3505
|
-
<infon key="type">Gene</infon>
|
|
3506
|
-
<location offset="30186" length="6"/>
|
|
3507
|
-
<text>ndc1-1</text>
|
|
3508
|
-
</annotation>
|
|
3509
|
-
<annotation id="G202">
|
|
3510
|
-
<infon key="type">Gene</infon>
|
|
3511
|
-
<location offset="30295" length="18"/>
|
|
3512
|
-
<text>pom152 null allele</text>
|
|
3513
|
-
</annotation>
|
|
3514
|
-
<annotation id="G203">
|
|
3515
|
-
<infon key="type">Gene</infon>
|
|
3516
|
-
<location offset="30329" length="10"/>
|
|
3517
|
-
<text>ndc1-1 SPB</text>
|
|
3518
|
-
</annotation>
|
|
3519
|
-
<annotation id="TEAM_277_67">
|
|
3520
|
-
<infon key="type">Gene</infon>
|
|
3521
|
-
<location offset="29308" length="4"/>
|
|
3522
|
-
<text>NDC1</text>
|
|
3523
|
-
</annotation>
|
|
3524
|
-
<annotation id="TEAM_277_68">
|
|
3525
|
-
<infon key="type">Gene</infon>
|
|
3526
|
-
<location offset="29507" length="6"/>
|
|
3527
|
-
<text>POM152</text>
|
|
3528
|
-
</annotation>
|
|
3529
|
-
<annotation id="TEAM_277_69">
|
|
3530
|
-
<infon key="type">Gene</infon>
|
|
3531
|
-
<location offset="30107" length="9"/>
|
|
3532
|
-
<text>Fig. 6 B</text>
|
|
3533
|
-
</annotation>
|
|
3534
|
-
<annotation id="TEAM_277_70">
|
|
3535
|
-
<infon key="type">Gene</infon>
|
|
3536
|
-
<location offset="30262" length="8"/>
|
|
3537
|
-
<text>Fig. 6 B</text>
|
|
3538
|
-
</annotation>
|
|
3539
1892
|
</passage>
|
|
3540
1893
|
<passage>
|
|
3541
1894
|
<infon key="type">title_1</infon>
|
|
@@ -3575,27 +1928,6 @@
|
|
|
3575
1928
|
<location offset="30952" length="5"/>
|
|
3576
1929
|
<text>Ndc1p</text>
|
|
3577
1930
|
</annotation>
|
|
3578
|
-
<annotation id="G204">
|
|
3579
|
-
<infon key="score">0.73</infon>
|
|
3580
|
-
<infon key="GeneID">854977</infon>
|
|
3581
|
-
<infon key="type">Gene</infon>
|
|
3582
|
-
<location offset="30737" length="5"/>
|
|
3583
|
-
<text>Ndc1p</text>
|
|
3584
|
-
</annotation>
|
|
3585
|
-
<annotation id="G205">
|
|
3586
|
-
<infon key="score">0.73</infon>
|
|
3587
|
-
<infon key="GeneID">854977</infon>
|
|
3588
|
-
<infon key="type">Gene</infon>
|
|
3589
|
-
<location offset="30832" length="5"/>
|
|
3590
|
-
<text>Ndc1p</text>
|
|
3591
|
-
</annotation>
|
|
3592
|
-
<annotation id="G206">
|
|
3593
|
-
<infon key="score">0.73</infon>
|
|
3594
|
-
<infon key="GeneID">854977</infon>
|
|
3595
|
-
<infon key="type">Gene</infon>
|
|
3596
|
-
<location offset="30952" length="5"/>
|
|
3597
|
-
<text>Ndc1p</text>
|
|
3598
|
-
</annotation>
|
|
3599
1931
|
</passage>
|
|
3600
1932
|
<passage>
|
|
3601
1933
|
<infon key="type">paragraph</infon>
|
|
@@ -3619,27 +1951,6 @@
|
|
|
3619
1951
|
<location offset="31642" length="5"/>
|
|
3620
1952
|
<text>Ndc1p</text>
|
|
3621
1953
|
</annotation>
|
|
3622
|
-
<annotation id="G207">
|
|
3623
|
-
<infon key="score">0.73</infon>
|
|
3624
|
-
<infon key="GeneID">854977</infon>
|
|
3625
|
-
<infon key="type">Gene</infon>
|
|
3626
|
-
<location offset="31038" length="5"/>
|
|
3627
|
-
<text>Ndc1p</text>
|
|
3628
|
-
</annotation>
|
|
3629
|
-
<annotation id="G208">
|
|
3630
|
-
<infon key="score">0.73</infon>
|
|
3631
|
-
<infon key="GeneID">854977</infon>
|
|
3632
|
-
<infon key="type">Gene</infon>
|
|
3633
|
-
<location offset="31420" length="5"/>
|
|
3634
|
-
<text>Ndc1p</text>
|
|
3635
|
-
</annotation>
|
|
3636
|
-
<annotation id="G209">
|
|
3637
|
-
<infon key="score">0.73</infon>
|
|
3638
|
-
<infon key="GeneID">854977</infon>
|
|
3639
|
-
<infon key="type">Gene</infon>
|
|
3640
|
-
<location offset="31642" length="5"/>
|
|
3641
|
-
<text>Ndc1p</text>
|
|
3642
|
-
</annotation>
|
|
3643
1954
|
</passage>
|
|
3644
1955
|
<passage>
|
|
3645
1956
|
<infon key="type">paragraph</infon>
|
|
@@ -3669,43 +1980,6 @@
|
|
|
3669
1980
|
<location offset="32511" length="5"/>
|
|
3670
1981
|
<text>Ndc1p</text>
|
|
3671
1982
|
</annotation>
|
|
3672
|
-
<annotation id="G210">
|
|
3673
|
-
<infon key="type">Gene</infon>
|
|
3674
|
-
<location offset="31855" length="10"/>
|
|
3675
|
-
<text>Ndc1p- GFP</text>
|
|
3676
|
-
</annotation>
|
|
3677
|
-
<annotation id="G211">
|
|
3678
|
-
<infon key="score">0.73</infon>
|
|
3679
|
-
<infon key="GeneID">854977</infon>
|
|
3680
|
-
<infon key="type">Gene</infon>
|
|
3681
|
-
<location offset="31962" length="9"/>
|
|
3682
|
-
<text>Ndc1p-GFP</text>
|
|
3683
|
-
</annotation>
|
|
3684
|
-
<annotation id="G212">
|
|
3685
|
-
<infon key="score">0.73</infon>
|
|
3686
|
-
<infon key="GeneID">854977</infon>
|
|
3687
|
-
<infon key="type">Gene</infon>
|
|
3688
|
-
<location offset="32151" length="9"/>
|
|
3689
|
-
<text>Ndc1p-GFP</text>
|
|
3690
|
-
</annotation>
|
|
3691
|
-
<annotation id="G213">
|
|
3692
|
-
<infon key="score">0.73</infon>
|
|
3693
|
-
<infon key="GeneID">854977</infon>
|
|
3694
|
-
<infon key="type">Gene</infon>
|
|
3695
|
-
<location offset="32511" length="5"/>
|
|
3696
|
-
<text>Ndc1p</text>
|
|
3697
|
-
</annotation>
|
|
3698
|
-
<annotation id="TEAM_277_71">
|
|
3699
|
-
<infon key="type">Gene</infon>
|
|
3700
|
-
<location offset="31962" length="9"/>
|
|
3701
|
-
<text>Ndc1p-GFP</text>
|
|
3702
|
-
</annotation>
|
|
3703
|
-
<annotation id="4">
|
|
3704
|
-
<infon key="type">ExperimentalMethod</infon>
|
|
3705
|
-
<infon key="PSIMI">0040</infon>
|
|
3706
|
-
<location offset="31755" length="84"/>
|
|
3707
|
-
<text>However, SPBs and NPCs are most clearly distinguished by using electron microscopy.</text>
|
|
3708
|
-
</annotation>
|
|
3709
1983
|
</passage>
|
|
3710
1984
|
<passage>
|
|
3711
1985
|
<infon key="type">paragraph</infon>
|
|
@@ -3723,54 +1997,6 @@
|
|
|
3723
1997
|
<location offset="32855" length="4"/>
|
|
3724
1998
|
<text>NDC1</text>
|
|
3725
1999
|
</annotation>
|
|
3726
|
-
<annotation id="G214">
|
|
3727
|
-
<infon key="score">0.73</infon>
|
|
3728
|
-
<infon key="GeneID">2542161</infon>
|
|
3729
|
-
<infon key="type">Gene</infon>
|
|
3730
|
-
<location offset="32613" length="11"/>
|
|
3731
|
-
<text>cut11+ gene</text>
|
|
3732
|
-
</annotation>
|
|
3733
|
-
<annotation id="G215">
|
|
3734
|
-
<infon key="score">0.70</infon>
|
|
3735
|
-
<infon key="GeneID">335236</infon>
|
|
3736
|
-
<infon key="type">Gene</infon>
|
|
3737
|
-
<location offset="32661" length="4"/>
|
|
3738
|
-
<text>NDC1</text>
|
|
3739
|
-
</annotation>
|
|
3740
|
-
<annotation id="G216">
|
|
3741
|
-
<infon key="type">Gene</infon>
|
|
3742
|
-
<location offset="32799" length="6"/>
|
|
3743
|
-
<text>Cut11p</text>
|
|
3744
|
-
</annotation>
|
|
3745
|
-
<annotation id="G217">
|
|
3746
|
-
<infon key="score">0.73</infon>
|
|
3747
|
-
<infon key="GeneID">2542161</infon>
|
|
3748
|
-
<infon key="type">Gene</infon>
|
|
3749
|
-
<location offset="32844" length="6"/>
|
|
3750
|
-
<text>cut11+</text>
|
|
3751
|
-
</annotation>
|
|
3752
|
-
<annotation id="G218">
|
|
3753
|
-
<infon key="score">0.70</infon>
|
|
3754
|
-
<infon key="GeneID">335236</infon>
|
|
3755
|
-
<infon key="type">Gene</infon>
|
|
3756
|
-
<location offset="32855" length="4"/>
|
|
3757
|
-
<text>NDC1</text>
|
|
3758
|
-
</annotation>
|
|
3759
|
-
<annotation id="G219">
|
|
3760
|
-
<infon key="type">Gene</infon>
|
|
3761
|
-
<location offset="32944" length="6"/>
|
|
3762
|
-
<text>Cut11p</text>
|
|
3763
|
-
</annotation>
|
|
3764
|
-
<annotation id="TEAM_277_72">
|
|
3765
|
-
<infon key="type">Gene</infon>
|
|
3766
|
-
<location offset="32661" length="4"/>
|
|
3767
|
-
<text>NDC1</text>
|
|
3768
|
-
</annotation>
|
|
3769
|
-
<annotation id="TEAM_277_73">
|
|
3770
|
-
<infon key="type">Gene</infon>
|
|
3771
|
-
<location offset="32855" length="4"/>
|
|
3772
|
-
<text>NDC1</text>
|
|
3773
|
-
</annotation>
|
|
3774
2000
|
</passage>
|
|
3775
2001
|
<passage>
|
|
3776
2002
|
<infon key="type">paragraph</infon>
|
|
@@ -3811,77 +2037,6 @@
|
|
|
3811
2037
|
<location offset="33879" length="4"/>
|
|
3812
2038
|
<text>NDC1</text>
|
|
3813
2039
|
</annotation>
|
|
3814
|
-
<annotation id="G220">
|
|
3815
|
-
<infon key="score">0.73</infon>
|
|
3816
|
-
<infon key="GeneID">854977</infon>
|
|
3817
|
-
<infon key="type">Gene</infon>
|
|
3818
|
-
<location offset="32999" length="5"/>
|
|
3819
|
-
<text>Ndc1p</text>
|
|
3820
|
-
</annotation>
|
|
3821
|
-
<annotation id="G221">
|
|
3822
|
-
<infon key="type">Gene</infon>
|
|
3823
|
-
<location offset="33338" length="13"/>
|
|
3824
|
-
<text>ndc1-1 allele</text>
|
|
3825
|
-
</annotation>
|
|
3826
|
-
<annotation id="G222">
|
|
3827
|
-
<infon key="score">0.70</infon>
|
|
3828
|
-
<infon key="GeneID">335236</infon>
|
|
3829
|
-
<infon key="type">Gene</infon>
|
|
3830
|
-
<location offset="33424" length="4"/>
|
|
3831
|
-
<text>NDC1</text>
|
|
3832
|
-
</annotation>
|
|
3833
|
-
<annotation id="G223">
|
|
3834
|
-
<infon key="score">0.70</infon>
|
|
3835
|
-
<infon key="GeneID">335236</infon>
|
|
3836
|
-
<infon key="type">Gene</infon>
|
|
3837
|
-
<location offset="33478" length="9"/>
|
|
3838
|
-
<text>NDC1 gene</text>
|
|
3839
|
-
</annotation>
|
|
3840
|
-
<annotation id="G224">
|
|
3841
|
-
<infon key="type">Gene</infon>
|
|
3842
|
-
<location offset="33510" length="13"/>
|
|
3843
|
-
<text>ndc1-1 allele</text>
|
|
3844
|
-
</annotation>
|
|
3845
|
-
<annotation id="G225">
|
|
3846
|
-
<infon key="score">0.70</infon>
|
|
3847
|
-
<infon key="GeneID">335236</infon>
|
|
3848
|
-
<infon key="type">Gene</infon>
|
|
3849
|
-
<location offset="33855" length="4"/>
|
|
3850
|
-
<text>NDC1</text>
|
|
3851
|
-
</annotation>
|
|
3852
|
-
<annotation id="G226">
|
|
3853
|
-
<infon key="score">0.70</infon>
|
|
3854
|
-
<infon key="GeneID">335236</infon>
|
|
3855
|
-
<infon key="type">Gene</infon>
|
|
3856
|
-
<location offset="33879" length="4"/>
|
|
3857
|
-
<text>NDC1</text>
|
|
3858
|
-
</annotation>
|
|
3859
|
-
<annotation id="TEAM_277_74">
|
|
3860
|
-
<infon key="type">Gene</infon>
|
|
3861
|
-
<infon key="GeneID">10277</infon>
|
|
3862
|
-
<location offset="33012" length="3"/>
|
|
3863
|
-
<text>SPB</text>
|
|
3864
|
-
</annotation>
|
|
3865
|
-
<annotation id="TEAM_277_75">
|
|
3866
|
-
<infon key="type">Gene</infon>
|
|
3867
|
-
<location offset="33424" length="4"/>
|
|
3868
|
-
<text>NDC1</text>
|
|
3869
|
-
</annotation>
|
|
3870
|
-
<annotation id="TEAM_277_76">
|
|
3871
|
-
<infon key="type">Gene</infon>
|
|
3872
|
-
<location offset="33478" length="4"/>
|
|
3873
|
-
<text>NDC1</text>
|
|
3874
|
-
</annotation>
|
|
3875
|
-
<annotation id="TEAM_277_77">
|
|
3876
|
-
<infon key="type">Gene</infon>
|
|
3877
|
-
<location offset="33855" length="4"/>
|
|
3878
|
-
<text>NDC1</text>
|
|
3879
|
-
</annotation>
|
|
3880
|
-
<annotation id="TEAM_277_78">
|
|
3881
|
-
<infon key="type">Gene</infon>
|
|
3882
|
-
<location offset="33879" length="4"/>
|
|
3883
|
-
<text>NDC1</text>
|
|
3884
|
-
</annotation>
|
|
3885
2040
|
</passage>
|
|
3886
2041
|
<passage>
|
|
3887
2042
|
<infon key="type">paragraph</infon>
|
|
@@ -4010,127 +2165,6 @@
|
|
|
4010
2165
|
<location offset="34820" length="187"/>
|
|
4011
2166
|
<text>Additionally, synthetic lethal interactions have been identified between mutant alleles of NIC96, NUP188, and POM152 (Aitchison et al., 1995b; Nehrbass et al., 1996; Zabel et al., 1996)</text>
|
|
4012
2167
|
</annotation>
|
|
4013
|
-
<annotation id="G227">
|
|
4014
|
-
<infon key="score">0.70</infon>
|
|
4015
|
-
<infon key="GeneID">850552</infon>
|
|
4016
|
-
<infon key="type">Gene</infon>
|
|
4017
|
-
<location offset="34100" length="10"/>
|
|
4018
|
-
<text>NIC96 gene</text>
|
|
4019
|
-
</annotation>
|
|
4020
|
-
<annotation id="G228">
|
|
4021
|
-
<infon key="score">0.70</infon>
|
|
4022
|
-
<infon key="GeneID">850552</infon>
|
|
4023
|
-
<infon key="type">Gene</infon>
|
|
4024
|
-
<location offset="34379" length="5"/>
|
|
4025
|
-
<text>NIC96</text>
|
|
4026
|
-
</annotation>
|
|
4027
|
-
<annotation id="G229">
|
|
4028
|
-
<infon key="score">0.58</infon>
|
|
4029
|
-
<infon key="GeneID">380283</infon>
|
|
4030
|
-
<infon key="type">Gene</infon>
|
|
4031
|
-
<location offset="34393" length="5"/>
|
|
4032
|
-
<text>NUP93</text>
|
|
4033
|
-
</annotation>
|
|
4034
|
-
<annotation id="G230">
|
|
4035
|
-
<infon key="type">Gene</infon>
|
|
4036
|
-
<location offset="34464" length="6"/>
|
|
4037
|
-
<text>Nup93p</text>
|
|
4038
|
-
</annotation>
|
|
4039
|
-
<annotation id="G231">
|
|
4040
|
-
<infon key="score">0.73</infon>
|
|
4041
|
-
<infon key="GeneID">850552</infon>
|
|
4042
|
-
<infon key="type">Gene</infon>
|
|
4043
|
-
<location offset="34590" length="6"/>
|
|
4044
|
-
<text>Nic96p</text>
|
|
4045
|
-
</annotation>
|
|
4046
|
-
<annotation id="G232">
|
|
4047
|
-
<infon key="score">0.73</infon>
|
|
4048
|
-
<infon key="GeneID">853409</infon>
|
|
4049
|
-
<infon key="type">Gene</infon>
|
|
4050
|
-
<location offset="34692" length="5"/>
|
|
4051
|
-
<text>Nsp1p</text>
|
|
4052
|
-
</annotation>
|
|
4053
|
-
<annotation id="G233">
|
|
4054
|
-
<infon key="score">0.73</infon>
|
|
4055
|
-
<infon key="GeneID">852703</infon>
|
|
4056
|
-
<infon key="type">Gene</infon>
|
|
4057
|
-
<location offset="34699" length="6"/>
|
|
4058
|
-
<text>Nup49p</text>
|
|
4059
|
-
</annotation>
|
|
4060
|
-
<annotation id="G234">
|
|
4061
|
-
<infon key="score">0.73</infon>
|
|
4062
|
-
<infon key="GeneID">853016</infon>
|
|
4063
|
-
<infon key="type">Gene</infon>
|
|
4064
|
-
<location offset="34711" length="6"/>
|
|
4065
|
-
<text>Nup57p</text>
|
|
4066
|
-
</annotation>
|
|
4067
|
-
<annotation id="G235">
|
|
4068
|
-
<infon key="score">0.70</infon>
|
|
4069
|
-
<infon key="GeneID">850552</infon>
|
|
4070
|
-
<infon key="type">Gene</infon>
|
|
4071
|
-
<location offset="34912" length="5"/>
|
|
4072
|
-
<text>NIC96</text>
|
|
4073
|
-
</annotation>
|
|
4074
|
-
<annotation id="G236">
|
|
4075
|
-
<infon key="score">0.58</infon>
|
|
4076
|
-
<infon key="GeneID">100135081</infon>
|
|
4077
|
-
<infon key="type">Gene</infon>
|
|
4078
|
-
<location offset="34919" length="6"/>
|
|
4079
|
-
<text>NUP188</text>
|
|
4080
|
-
</annotation>
|
|
4081
|
-
<annotation id="TEAM_277_79">
|
|
4082
|
-
<infon key="type">Gene</infon>
|
|
4083
|
-
<infon key="GeneID">224119</infon>
|
|
4084
|
-
<location offset="34100" length="5"/>
|
|
4085
|
-
<text>NIC96</text>
|
|
4086
|
-
</annotation>
|
|
4087
|
-
<annotation id="TEAM_277_80">
|
|
4088
|
-
<infon key="type">Gene</infon>
|
|
4089
|
-
<infon key="GeneID">224119</infon>
|
|
4090
|
-
<location offset="34379" length="5"/>
|
|
4091
|
-
<text>NIC96</text>
|
|
4092
|
-
</annotation>
|
|
4093
|
-
<annotation id="TEAM_277_81">
|
|
4094
|
-
<infon key="type">Gene</infon>
|
|
4095
|
-
<infon key="GeneID">4415</infon>
|
|
4096
|
-
<location offset="34393" length="5"/>
|
|
4097
|
-
<text>NUP93</text>
|
|
4098
|
-
</annotation>
|
|
4099
|
-
<annotation id="TEAM_277_82">
|
|
4100
|
-
<infon key="type">Gene</infon>
|
|
4101
|
-
<infon key="GeneID">4415</infon>
|
|
4102
|
-
<location offset="34464" length="6"/>
|
|
4103
|
-
<text>Nup93p</text>
|
|
4104
|
-
</annotation>
|
|
4105
|
-
<annotation id="TEAM_277_83">
|
|
4106
|
-
<infon key="type">Gene</infon>
|
|
4107
|
-
<infon key="GeneID">461059</infon>
|
|
4108
|
-
<location offset="34692" length="5"/>
|
|
4109
|
-
<text>Nsp1p</text>
|
|
4110
|
-
</annotation>
|
|
4111
|
-
<annotation id="TEAM_277_84">
|
|
4112
|
-
<infon key="type">Gene</infon>
|
|
4113
|
-
<infon key="GeneID">4415</infon>
|
|
4114
|
-
<location offset="34699" length="6"/>
|
|
4115
|
-
<text>Nup49p</text>
|
|
4116
|
-
</annotation>
|
|
4117
|
-
<annotation id="TEAM_277_85">
|
|
4118
|
-
<infon key="type">Gene</infon>
|
|
4119
|
-
<infon key="GeneID">224119</infon>
|
|
4120
|
-
<location offset="34912" length="5"/>
|
|
4121
|
-
<text>NIC96</text>
|
|
4122
|
-
</annotation>
|
|
4123
|
-
<annotation id="TEAM_277_86">
|
|
4124
|
-
<infon key="type">Gene</infon>
|
|
4125
|
-
<infon key="GeneID">4415</infon>
|
|
4126
|
-
<location offset="34919" length="6"/>
|
|
4127
|
-
<text>NUP188</text>
|
|
4128
|
-
</annotation>
|
|
4129
|
-
<annotation id="TEAM_277_87">
|
|
4130
|
-
<infon key="type">Gene</infon>
|
|
4131
|
-
<location offset="34931" length="6"/>
|
|
4132
|
-
<text>POM152</text>
|
|
4133
|
-
</annotation>
|
|
4134
2168
|
</passage>
|
|
4135
2169
|
<passage>
|
|
4136
2170
|
<infon key="type">paragraph</infon>
|
|
@@ -4176,52 +2210,6 @@
|
|
|
4176
2210
|
<location offset="35495" length="172"/>
|
|
4177
2211
|
<text>Because a function for Ndc1p at NPCs has not been determined, we do not know if the suppression of ndc1-1 by the pom152 null allele has any functional consequence for NPCs</text>
|
|
4178
2212
|
</annotation>
|
|
4179
|
-
<annotation id="G237">
|
|
4180
|
-
<infon key="type">Gene</infon>
|
|
4181
|
-
<location offset="35084" length="18"/>
|
|
4182
|
-
<text>pom152 null allele</text>
|
|
4183
|
-
</annotation>
|
|
4184
|
-
<annotation id="G238">
|
|
4185
|
-
<infon key="score">0.73</infon>
|
|
4186
|
-
<infon key="GeneID">855159</infon>
|
|
4187
|
-
<infon key="type">Gene</infon>
|
|
4188
|
-
<location offset="35243" length="7"/>
|
|
4189
|
-
<text>Pom152p</text>
|
|
4190
|
-
</annotation>
|
|
4191
|
-
<annotation id="G239">
|
|
4192
|
-
<infon key="type">Gene</infon>
|
|
4193
|
-
<location offset="35318" length="10"/>
|
|
4194
|
-
<text>ndc1-1 SPB</text>
|
|
4195
|
-
</annotation>
|
|
4196
|
-
<annotation id="G240">
|
|
4197
|
-
<infon key="type">Gene</infon>
|
|
4198
|
-
<location offset="35388" length="10"/>
|
|
4199
|
-
<text>ndc1-1 SPB</text>
|
|
4200
|
-
</annotation>
|
|
4201
|
-
<annotation id="G241">
|
|
4202
|
-
<infon key="score">0.73</infon>
|
|
4203
|
-
<infon key="GeneID">854977</infon>
|
|
4204
|
-
<infon key="type">Gene</infon>
|
|
4205
|
-
<location offset="35518" length="5"/>
|
|
4206
|
-
<text>Ndc1p</text>
|
|
4207
|
-
</annotation>
|
|
4208
|
-
<annotation id="G242">
|
|
4209
|
-
<infon key="type">Gene</infon>
|
|
4210
|
-
<location offset="35595" length="6"/>
|
|
4211
|
-
<text>ndc1-1</text>
|
|
4212
|
-
</annotation>
|
|
4213
|
-
<annotation id="G243">
|
|
4214
|
-
<infon key="type">Gene</infon>
|
|
4215
|
-
<location offset="35609" length="18"/>
|
|
4216
|
-
<text>pom152 null allele</text>
|
|
4217
|
-
</annotation>
|
|
4218
|
-
<annotation id="G244">
|
|
4219
|
-
<infon key="score">0.73</infon>
|
|
4220
|
-
<infon key="GeneID">443043</infon>
|
|
4221
|
-
<infon key="type">Gene</infon>
|
|
4222
|
-
<location offset="35730" length="4"/>
|
|
4223
|
-
<text>SPBs</text>
|
|
4224
|
-
</annotation>
|
|
4225
2213
|
</passage>
|
|
4226
2214
|
<passage>
|
|
4227
2215
|
<infon key="type">paragraph</infon>
|
|
@@ -4311,79 +2299,6 @@
|
|
|
4311
2299
|
<location offset="35824" length="178"/>
|
|
4312
2300
|
<text>The mechanism by which the pom152 null allele suppresses the ndc1-1 SPB duplication defect is not known, but it appears as if wild-type POM152 is antagonistic in ndc1-1 strains</text>
|
|
4313
2301
|
</annotation>
|
|
4314
|
-
<annotation id="G245">
|
|
4315
|
-
<infon key="type">Gene</infon>
|
|
4316
|
-
<location offset="35851" length="18"/>
|
|
4317
|
-
<text>pom152 null allele</text>
|
|
4318
|
-
</annotation>
|
|
4319
|
-
<annotation id="G246">
|
|
4320
|
-
<infon key="type">Gene</infon>
|
|
4321
|
-
<location offset="35885" length="10"/>
|
|
4322
|
-
<text>ndc1-1 SPB</text>
|
|
4323
|
-
</annotation>
|
|
4324
|
-
<annotation id="G247">
|
|
4325
|
-
<infon key="type">Gene</infon>
|
|
4326
|
-
<location offset="35988" length="6"/>
|
|
4327
|
-
<text>ndc1-1</text>
|
|
4328
|
-
</annotation>
|
|
4329
|
-
<annotation id="G248">
|
|
4330
|
-
<infon key="score">0.73</infon>
|
|
4331
|
-
<infon key="GeneID">855159</infon>
|
|
4332
|
-
<infon key="type">Gene</infon>
|
|
4333
|
-
<location offset="36083" length="7"/>
|
|
4334
|
-
<text>Pom152p</text>
|
|
4335
|
-
</annotation>
|
|
4336
|
-
<annotation id="G249">
|
|
4337
|
-
<infon key="score">0.70</infon>
|
|
4338
|
-
<infon key="GeneID">855159</infon>
|
|
4339
|
-
<infon key="type">Gene</infon>
|
|
4340
|
-
<location offset="36172" length="11"/>
|
|
4341
|
-
<text>POM152 gene</text>
|
|
4342
|
-
</annotation>
|
|
4343
|
-
<annotation id="G250">
|
|
4344
|
-
<infon key="score">0.70</infon>
|
|
4345
|
-
<infon key="GeneID">855159</infon>
|
|
4346
|
-
<infon key="type">Gene</infon>
|
|
4347
|
-
<location offset="36225" length="6"/>
|
|
4348
|
-
<text>POM152</text>
|
|
4349
|
-
</annotation>
|
|
4350
|
-
<annotation id="G251">
|
|
4351
|
-
<infon key="score">0.70</infon>
|
|
4352
|
-
<infon key="GeneID">855159</infon>
|
|
4353
|
-
<infon key="type">Gene</infon>
|
|
4354
|
-
<location offset="36328" length="6"/>
|
|
4355
|
-
<text>POM152</text>
|
|
4356
|
-
</annotation>
|
|
4357
|
-
<annotation id="TEAM_277_88">
|
|
4358
|
-
<infon key="type">Gene</infon>
|
|
4359
|
-
<location offset="35851" length="6"/>
|
|
4360
|
-
<text>pom152</text>
|
|
4361
|
-
</annotation>
|
|
4362
|
-
<annotation id="TEAM_277_89">
|
|
4363
|
-
<infon key="type">Gene</infon>
|
|
4364
|
-
<location offset="35961" length="6"/>
|
|
4365
|
-
<text>POM152</text>
|
|
4366
|
-
</annotation>
|
|
4367
|
-
<annotation id="TEAM_277_90">
|
|
4368
|
-
<infon key="type">Gene</infon>
|
|
4369
|
-
<location offset="36172" length="6"/>
|
|
4370
|
-
<text>POM152</text>
|
|
4371
|
-
</annotation>
|
|
4372
|
-
<annotation id="TEAM_277_91">
|
|
4373
|
-
<infon key="type">Gene</infon>
|
|
4374
|
-
<location offset="36225" length="6"/>
|
|
4375
|
-
<text>POM152</text>
|
|
4376
|
-
</annotation>
|
|
4377
|
-
<annotation id="TEAM_277_92">
|
|
4378
|
-
<infon key="type">Gene</infon>
|
|
4379
|
-
<location offset="36328" length="6"/>
|
|
4380
|
-
<text>POM152</text>
|
|
4381
|
-
</annotation>
|
|
4382
|
-
<annotation id="TEAM_277_93">
|
|
4383
|
-
<infon key="type">Gene</infon>
|
|
4384
|
-
<location offset="36464" length="6"/>
|
|
4385
|
-
<text>POM152</text>
|
|
4386
|
-
</annotation>
|
|
4387
2302
|
</passage>
|
|
4388
2303
|
<passage>
|
|
4389
2304
|
<infon key="type">paragraph</infon>
|
|
@@ -4402,101 +2317,34 @@
|
|
|
4402
2317
|
<text>Ndc1p</text>
|
|
4403
2318
|
</annotation>
|
|
4404
2319
|
<annotation id="C363">
|
|
4405
|
-
<infon key="NCBI Gene">855159</infon>
|
|
4406
|
-
<infon key="type">Gene</infon>
|
|
4407
|
-
<location offset="37053" length="7"/>
|
|
4408
|
-
<text>Pom152p</text>
|
|
4409
|
-
</annotation>
|
|
4410
|
-
<annotation id="C364">
|
|
4411
|
-
<infon key="NCBI Gene">855159</infon>
|
|
4412
|
-
<infon key="type">Gene</infon>
|
|
4413
|
-
<location offset="37267" length="7"/>
|
|
4414
|
-
<text>Pom152p</text>
|
|
4415
|
-
</annotation>
|
|
4416
|
-
<annotation id="C365">
|
|
4417
|
-
<infon key="NCBI Gene">855159</infon>
|
|
4418
|
-
<infon key="type">Gene</infon>
|
|
4419
|
-
<location offset="37329" length="7"/>
|
|
4420
|
-
<text>Pom152p</text>
|
|
4421
|
-
</annotation>
|
|
4422
|
-
<annotation id="C366">
|
|
4423
|
-
<infon key="NCBI Gene">855159</infon>
|
|
4424
|
-
<infon key="type">Gene</infon>
|
|
4425
|
-
<location offset="37512" length="7"/>
|
|
4426
|
-
<text>Pom152p</text>
|
|
4427
|
-
</annotation>
|
|
4428
|
-
<annotation id="E15">
|
|
4429
|
-
<infon key="score">0.080469183</infon>
|
|
4430
|
-
<infon key="type">PPImention</infon>
|
|
4431
|
-
<location offset="37023" length="229"/>
|
|
4432
|
-
<text>It is possible that Ndc1p and Pom152p may directly interact at NPCs, because immunoelectron microscopy shows that these two proteins localize to similar regions of the NPC (Wozniak et al., 1994), and both are membrane proteins</text>
|
|
4433
|
-
</annotation>
|
|
4434
|
-
<annotation id="G252">
|
|
4435
|
-
<infon key="score">0.73</infon>
|
|
4436
|
-
<infon key="GeneID">855159</infon>
|
|
4437
|
-
<infon key="type">Gene</infon>
|
|
4438
|
-
<location offset="36514" length="7"/>
|
|
4439
|
-
<text>Pom152p</text>
|
|
4440
|
-
</annotation>
|
|
4441
|
-
<annotation id="G253">
|
|
4442
|
-
<infon key="type">Gene</infon>
|
|
4443
|
-
<location offset="36623" length="7"/>
|
|
4444
|
-
<text>Ndc1-1p</text>
|
|
4445
|
-
</annotation>
|
|
4446
|
-
<annotation id="G254">
|
|
4447
|
-
<infon key="type">Gene</infon>
|
|
4448
|
-
<location offset="36814" length="13"/>
|
|
4449
|
-
<text>ndc1-1 allele</text>
|
|
4450
|
-
</annotation>
|
|
4451
|
-
<annotation id="G255">
|
|
4452
|
-
<infon key="type">Gene</infon>
|
|
4453
|
-
<location offset="36841" length="6"/>
|
|
4454
|
-
<text>ndc1-1</text>
|
|
4455
|
-
</annotation>
|
|
4456
|
-
<annotation id="G256">
|
|
4457
|
-
<infon key="type">Gene</infon>
|
|
4458
|
-
<location offset="36890" length="7"/>
|
|
4459
|
-
<text>Ndc1-1p</text>
|
|
4460
|
-
</annotation>
|
|
4461
|
-
<annotation id="G257">
|
|
4462
|
-
<infon key="score">0.73</infon>
|
|
4463
|
-
<infon key="GeneID">854977</infon>
|
|
4464
|
-
<infon key="type">Gene</infon>
|
|
4465
|
-
<location offset="37043" length="5"/>
|
|
4466
|
-
<text>Ndc1p</text>
|
|
4467
|
-
</annotation>
|
|
4468
|
-
<annotation id="G258">
|
|
4469
|
-
<infon key="score">0.73</infon>
|
|
4470
|
-
<infon key="GeneID">855159</infon>
|
|
2320
|
+
<infon key="NCBI Gene">855159</infon>
|
|
4471
2321
|
<infon key="type">Gene</infon>
|
|
4472
2322
|
<location offset="37053" length="7"/>
|
|
4473
2323
|
<text>Pom152p</text>
|
|
4474
2324
|
</annotation>
|
|
4475
|
-
<annotation id="
|
|
4476
|
-
<infon key="
|
|
4477
|
-
<infon key="GeneID">855159</infon>
|
|
2325
|
+
<annotation id="C364">
|
|
2326
|
+
<infon key="NCBI Gene">855159</infon>
|
|
4478
2327
|
<infon key="type">Gene</infon>
|
|
4479
2328
|
<location offset="37267" length="7"/>
|
|
4480
2329
|
<text>Pom152p</text>
|
|
4481
2330
|
</annotation>
|
|
4482
|
-
<annotation id="
|
|
4483
|
-
<infon key="
|
|
4484
|
-
<infon key="GeneID">855159</infon>
|
|
2331
|
+
<annotation id="C365">
|
|
2332
|
+
<infon key="NCBI Gene">855159</infon>
|
|
4485
2333
|
<infon key="type">Gene</infon>
|
|
4486
2334
|
<location offset="37329" length="7"/>
|
|
4487
2335
|
<text>Pom152p</text>
|
|
4488
2336
|
</annotation>
|
|
4489
|
-
<annotation id="
|
|
4490
|
-
<infon key="
|
|
4491
|
-
<infon key="GeneID">855159</infon>
|
|
2337
|
+
<annotation id="C366">
|
|
2338
|
+
<infon key="NCBI Gene">855159</infon>
|
|
4492
2339
|
<infon key="type">Gene</infon>
|
|
4493
2340
|
<location offset="37512" length="7"/>
|
|
4494
2341
|
<text>Pom152p</text>
|
|
4495
2342
|
</annotation>
|
|
4496
|
-
<annotation id="
|
|
4497
|
-
<infon key="
|
|
4498
|
-
<
|
|
4499
|
-
<
|
|
2343
|
+
<annotation id="E15">
|
|
2344
|
+
<infon key="score">0.080469183</infon>
|
|
2345
|
+
<infon key="type">PPImention</infon>
|
|
2346
|
+
<location offset="37023" length="229"/>
|
|
2347
|
+
<text>It is possible that Ndc1p and Pom152p may directly interact at NPCs, because immunoelectron microscopy shows that these two proteins localize to similar regions of the NPC (Wozniak et al., 1994), and both are membrane proteins</text>
|
|
4500
2348
|
</annotation>
|
|
4501
2349
|
</passage>
|
|
4502
2350
|
<passage>
|
|
@@ -4509,13 +2357,6 @@
|
|
|
4509
2357
|
<location offset="37971" length="5"/>
|
|
4510
2358
|
<text>Ndc1p</text>
|
|
4511
2359
|
</annotation>
|
|
4512
|
-
<annotation id="G263">
|
|
4513
|
-
<infon key="score">0.73</infon>
|
|
4514
|
-
<infon key="GeneID">854977</infon>
|
|
4515
|
-
<infon key="type">Gene</infon>
|
|
4516
|
-
<location offset="37971" length="5"/>
|
|
4517
|
-
<text>Ndc1p</text>
|
|
4518
|
-
</annotation>
|
|
4519
2360
|
</passage>
|
|
4520
2361
|
<passage>
|
|
4521
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|
<infon key="type">title_1</infon>
|
|
@@ -5076,530 +2917,39 @@
|
|
|
5076
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|
</annotation>
|
|
5077
2918
|
<annotation id="C552">
|
|
5078
2919
|
<infon key="tmVar">|DEL||1</infon>
|
|
5079
|
-
<infon key="type">DNAMutation</infon>
|
|
5080
|
-
<location offset="39428" length="6"/>
|
|
5081
|
-
<text>Delta1</text>
|
|
5082
|
-
</annotation>
|
|
5083
|
-
<annotation id="C553">
|
|
5084
|
-
<infon key="tmVar">|DEL||1</infon>
|
|
5085
|
-
<infon key="type">DNAMutation</infon>
|
|
5086
|
-
<location offset="39417" length="6"/>
|
|
5087
|
-
<text>Delta1</text>
|
|
5088
|
-
</annotation>
|
|
5089
|
-
<annotation id="C554">
|
|
5090
|
-
<infon key="tmVar">p|SUB|S|65|T</infon>
|
|
5091
|
-
<infon key="type">ProteinMutation</infon>
|
|
5092
|
-
<location offset="38781" length="4"/>
|
|
5093
|
-
<text>S65T</text>
|
|
5094
|
-
</annotation>
|
|
5095
|
-
<annotation id="C555">
|
|
5096
|
-
<infon key="tmVar">p|SUB|S|65|T</infon>
|
|
5097
|
-
<infon key="type">ProteinMutation</infon>
|
|
5098
|
-
<location offset="38751" length="4"/>
|
|
5099
|
-
<text>S65T</text>
|
|
5100
|
-
</annotation>
|
|
5101
|
-
<annotation id="C556">
|
|
5102
|
-
<infon key="tmVar">p|SUB|S|65|T</infon>
|
|
5103
|
-
<infon key="type">ProteinMutation</infon>
|
|
5104
|
-
<location offset="38543" length="4"/>
|
|
5105
|
-
<text>S65T</text>
|
|
5106
|
-
</annotation>
|
|
5107
|
-
<annotation id="C557">
|
|
5108
|
-
<infon key="tmVar">p|SUB|S|65|T</infon>
|
|
5109
|
-
<infon key="type">ProteinMutation</infon>
|
|
5110
|
-
<location offset="38513" length="4"/>
|
|
5111
|
-
<text>S65T</text>
|
|
5112
|
-
</annotation>
|
|
5113
|
-
<annotation id="G264">
|
|
5114
|
-
<infon key="type">Gene</infon>
|
|
5115
|
-
<location offset="38142" length="325"/>
|
|
5116
|
-
<text>ProA5-4d/4a MATa/MATalpha NDC1-ProA-HIS3-URA3/NDC1-ProA-HIS3-URA3 ura3-52/ura3-52 his3Delta200/his3Delta200 trp1-1/trp1-1 leu2-3,112/leu2-3,112 lys2-801/lys2-801 ProA5-4d MATaNDC1-ProA-HIS3-URA3 ura3-52 his3Delta200 trp1-1 leu2-3,112 lys2-801 HC26-15a/1a MATa/MATalpha NDC1-ProA-HIS3-URA3/NDC1-ProA-HIS3-URA3 nup49-1</text>
|
|
5117
|
-
</annotation>
|
|
5118
|
-
<annotation id="G265">
|
|
5119
|
-
<infon key="type">Gene</infon>
|
|
5120
|
-
<location offset="38151" length="8"/>
|
|
5121
|
-
<text>4a MATa</text>
|
|
5122
|
-
</annotation>
|
|
5123
|
-
<annotation id="G266">
|
|
5124
|
-
<infon key="type">Gene</infon>
|
|
5125
|
-
<location offset="38160" length="28"/>
|
|
5126
|
-
<text>MATalpha NDC1-ProA-HIS3-URA3</text>
|
|
5127
|
-
</annotation>
|
|
5128
|
-
<annotation id="G267">
|
|
5129
|
-
<infon key="type">Gene</infon>
|
|
5130
|
-
<location offset="38189" length="27"/>
|
|
5131
|
-
<text>NDC1-ProA-HIS3-URA3 ura3-52</text>
|
|
5132
|
-
</annotation>
|
|
5133
|
-
<annotation id="G268">
|
|
5134
|
-
<infon key="type">Gene</infon>
|
|
5135
|
-
<location offset="38217" length="20"/>
|
|
5136
|
-
<text>ura3-52 his3Delta200</text>
|
|
5137
|
-
</annotation>
|
|
5138
|
-
<annotation id="G269">
|
|
5139
|
-
<infon key="type">Gene</infon>
|
|
5140
|
-
<location offset="38238" length="19"/>
|
|
5141
|
-
<text>his3Delta200 trp1-1</text>
|
|
5142
|
-
</annotation>
|
|
5143
|
-
<annotation id="G270">
|
|
5144
|
-
<infon key="type">Gene</infon>
|
|
5145
|
-
<location offset="38258" length="21"/>
|
|
5146
|
-
<text>trp1-1 leu2-3,112</text>
|
|
5147
|
-
</annotation>
|
|
5148
|
-
<annotation id="G271">
|
|
5149
|
-
<infon key="type">Gene</infon>
|
|
5150
|
-
<location offset="38280" length="19"/>
|
|
5151
|
-
<text>leu2-3,112 lys2-801</text>
|
|
5152
|
-
</annotation>
|
|
5153
|
-
<annotation id="G272">
|
|
5154
|
-
<infon key="type">Gene</infon>
|
|
5155
|
-
<location offset="38300" length="101"/>
|
|
5156
|
-
<text>lys2-801 ProA5-4d MATaNDC1-ProA-HIS3-URA3 ura3-52 his3Delta200 trp1-1 leu2-3,112 lys2-801 HC26-15a</text>
|
|
5157
|
-
</annotation>
|
|
5158
|
-
<annotation id="G273">
|
|
5159
|
-
<infon key="type">Gene</infon>
|
|
5160
|
-
<location offset="38402" length="8"/>
|
|
5161
|
-
<text>1a MATa</text>
|
|
5162
|
-
</annotation>
|
|
5163
|
-
<annotation id="G274">
|
|
5164
|
-
<infon key="type">Gene</infon>
|
|
5165
|
-
<location offset="38411" length="28"/>
|
|
5166
|
-
<text>MATalpha NDC1-ProA-HIS3-URA3</text>
|
|
5167
|
-
</annotation>
|
|
5168
|
-
<annotation id="G275">
|
|
5169
|
-
<infon key="type">Gene</infon>
|
|
5170
|
-
<location offset="38440" length="27"/>
|
|
5171
|
-
<text>NDC1-ProA-HIS3-URA3 nup49-1</text>
|
|
5172
|
-
</annotation>
|
|
5173
|
-
<annotation id="G276">
|
|
5174
|
-
<infon key="type">Gene</infon>
|
|
5175
|
-
<location offset="38483" length="19"/>
|
|
5176
|
-
<text>URA3 nup49DeltaGFLG</text>
|
|
5177
|
-
</annotation>
|
|
5178
|
-
<annotation id="G277">
|
|
5179
|
-
<infon key="type">Gene</infon>
|
|
5180
|
-
<location offset="38539" length="110"/>
|
|
5181
|
-
<text>GFP-S65T-TRP1 ura3/ura3 his3/his3 trp1-1/trp1-1 leu2-3,112/leu2-3,112 HC27-16d/16b MATa/MATalpha nup133Delta</text>
|
|
5182
|
-
</annotation>
|
|
5183
|
-
<annotation id="G278">
|
|
5184
|
-
<infon key="type">Gene</infon>
|
|
5185
|
-
<location offset="38558" length="9"/>
|
|
5186
|
-
<text>ura3 his3</text>
|
|
5187
|
-
</annotation>
|
|
5188
|
-
<annotation id="G279">
|
|
5189
|
-
<infon key="type">Gene</infon>
|
|
5190
|
-
<location offset="38568" length="11"/>
|
|
5191
|
-
<text>his3 trp1-1</text>
|
|
5192
|
-
</annotation>
|
|
5193
|
-
<annotation id="G280">
|
|
5194
|
-
<infon key="type">Gene</infon>
|
|
5195
|
-
<location offset="38580" length="17"/>
|
|
5196
|
-
<text>trp1-1 leu2-3,112</text>
|
|
5197
|
-
</annotation>
|
|
5198
|
-
<annotation id="G281">
|
|
5199
|
-
<infon key="type">Gene</infon>
|
|
5200
|
-
<location offset="38598" length="20"/>
|
|
5201
|
-
<text>leu2-3,112 HC27-16d</text>
|
|
5202
|
-
</annotation>
|
|
5203
|
-
<annotation id="G282">
|
|
5204
|
-
<infon key="type">Gene</infon>
|
|
5205
|
-
<location offset="38619" length="9"/>
|
|
5206
|
-
<text>16b MATa</text>
|
|
5207
|
-
</annotation>
|
|
5208
|
-
<annotation id="G283">
|
|
5209
|
-
<infon key="type">Gene</infon>
|
|
5210
|
-
<location offset="38629" length="20"/>
|
|
5211
|
-
<text>MATalpha nup133Delta</text>
|
|
5212
|
-
</annotation>
|
|
5213
|
-
<annotation id="G284">
|
|
5214
|
-
<infon key="type">Gene</infon>
|
|
5215
|
-
<location offset="38669" length="37"/>
|
|
5216
|
-
<text>HIS3 NDC1-ProA-HIS3-URA3/NDC1 nup49-1</text>
|
|
5217
|
-
</annotation>
|
|
5218
|
-
<annotation id="G285">
|
|
5219
|
-
<infon key="type">Gene</infon>
|
|
5220
|
-
<location offset="38722" length="23"/>
|
|
5221
|
-
<text>URA3 nup49DeltaGFLG</text>
|
|
5222
|
-
</annotation>
|
|
5223
|
-
<annotation id="G286">
|
|
5224
|
-
<infon key="type">Gene</infon>
|
|
5225
|
-
<location offset="38747" length="28"/>
|
|
5226
|
-
<text>GFP-S65T-TRP1/nup49DeltaGFLG</text>
|
|
5227
|
-
</annotation>
|
|
5228
|
-
<annotation id="G287">
|
|
5229
|
-
<infon key="type">Gene</infon>
|
|
5230
|
-
<location offset="38777" length="54"/>
|
|
5231
|
-
<text>GFP-S65T-TRP1 ura3/ura3 his3/his3 trp1-1/trp1-1 leu2-3</text>
|
|
5232
|
-
</annotation>
|
|
5233
|
-
<annotation id="G288">
|
|
5234
|
-
<infon key="type">Gene</infon>
|
|
5235
|
-
<location offset="38860" length="64"/>
|
|
5236
|
-
<text>MATa/MATalpha NDC1-ProA-HIS3-URA3/NDC1-ProA-HIS3-URA3 spc42Delta</text>
|
|
5237
|
-
</annotation>
|
|
5238
|
-
<annotation id="G289">
|
|
5239
|
-
<infon key="type">Gene</infon>
|
|
5240
|
-
<location offset="38865" length="28"/>
|
|
5241
|
-
<text>MATalpha NDC1-ProA-HIS3-URA3</text>
|
|
5242
|
-
</annotation>
|
|
5243
|
-
<annotation id="G290">
|
|
5244
|
-
<infon key="type">Gene</infon>
|
|
5245
|
-
<location offset="38894" length="30"/>
|
|
5246
|
-
<text>NDC1-ProA-HIS3-URA3 spc42Delta</text>
|
|
5247
|
-
</annotation>
|
|
5248
|
-
<annotation id="G291">
|
|
5249
|
-
<infon key="type">Gene</infon>
|
|
5250
|
-
<location offset="38926" length="15"/>
|
|
5251
|
-
<text>LEU2/spc42Delta</text>
|
|
5252
|
-
</annotation>
|
|
5253
|
-
<annotation id="G292">
|
|
5254
|
-
<infon key="type">Gene</infon>
|
|
5255
|
-
<location offset="38931" length="10"/>
|
|
5256
|
-
<text>spc42Delta</text>
|
|
5257
|
-
</annotation>
|
|
5258
|
-
<annotation id="G293">
|
|
5259
|
-
<infon key="type">Gene</infon>
|
|
5260
|
-
<location offset="38943" length="13"/>
|
|
5261
|
-
<text>LEU2 TRP1</text>
|
|
5262
|
-
</annotation>
|
|
5263
|
-
<annotation id="G294">
|
|
5264
|
-
<infon key="score">0.73</infon>
|
|
5265
|
-
<infon key="GeneID">853824</infon>
|
|
5266
|
-
<infon key="type">Gene</infon>
|
|
5267
|
-
<location offset="38958" length="9"/>
|
|
5268
|
-
<text>SPC42-GFP</text>
|
|
5269
|
-
</annotation>
|
|
5270
|
-
<annotation id="G295">
|
|
5271
|
-
<infon key="type">Gene</infon>
|
|
5272
|
-
<location offset="38978" length="165"/>
|
|
5273
|
-
<text>SPC42-GFP(3x) ura3/ura3 his3/his3 trp1-1/trp1-1 leu2-3,112/leu2-3,112 ade2-1/ade2-1 HC23-11d/16a MATa/MATalpha NIC96-ProA-HIS3-URA3/NIC96-ProA-HIS3-URA3 spc42Delta</text>
|
|
5274
|
-
</annotation>
|
|
5275
|
-
<annotation id="G296">
|
|
5276
|
-
<infon key="type">Gene</infon>
|
|
5277
|
-
<location offset="38997" length="9"/>
|
|
5278
|
-
<text>ura3 his3</text>
|
|
5279
|
-
</annotation>
|
|
5280
|
-
<annotation id="G297">
|
|
5281
|
-
<infon key="type">Gene</infon>
|
|
5282
|
-
<location offset="39007" length="11"/>
|
|
5283
|
-
<text>his3 trp1-1</text>
|
|
5284
|
-
</annotation>
|
|
5285
|
-
<annotation id="G298">
|
|
5286
|
-
<infon key="type">Gene</infon>
|
|
5287
|
-
<location offset="39019" length="17"/>
|
|
5288
|
-
<text>trp1-1 leu2-3,112</text>
|
|
5289
|
-
</annotation>
|
|
5290
|
-
<annotation id="G299">
|
|
5291
|
-
<infon key="type">Gene</infon>
|
|
5292
|
-
<location offset="39037" length="17"/>
|
|
5293
|
-
<text>leu2-3,112 ade2-1</text>
|
|
5294
|
-
</annotation>
|
|
5295
|
-
<annotation id="G300">
|
|
5296
|
-
<infon key="type">Gene</infon>
|
|
5297
|
-
<location offset="39055" length="16"/>
|
|
5298
|
-
<text>ade2-1 HC23-11d</text>
|
|
5299
|
-
</annotation>
|
|
5300
|
-
<annotation id="G301">
|
|
5301
|
-
<infon key="type">Gene</infon>
|
|
5302
|
-
<location offset="39072" length="9"/>
|
|
5303
|
-
<text>16a MATa</text>
|
|
5304
|
-
</annotation>
|
|
5305
|
-
<annotation id="G302">
|
|
5306
|
-
<infon key="type">Gene</infon>
|
|
5307
|
-
<location offset="39082" length="29"/>
|
|
5308
|
-
<text>MATalpha NIC96-ProA-HIS3-URA3</text>
|
|
5309
|
-
</annotation>
|
|
5310
|
-
<annotation id="G303">
|
|
5311
|
-
<infon key="type">Gene</infon>
|
|
5312
|
-
<location offset="39112" length="31"/>
|
|
5313
|
-
<text>NIC96-ProA-HIS3-URA3 spc42Delta</text>
|
|
5314
|
-
</annotation>
|
|
5315
|
-
<annotation id="G304">
|
|
5316
|
-
<infon key="type">Gene</infon>
|
|
5317
|
-
<location offset="39145" length="15"/>
|
|
5318
|
-
<text>LEU2/spc42Delta</text>
|
|
5319
|
-
</annotation>
|
|
5320
|
-
<annotation id="G305">
|
|
5321
|
-
<infon key="type">Gene</infon>
|
|
5322
|
-
<location offset="39150" length="10"/>
|
|
5323
|
-
<text>spc42Delta</text>
|
|
5324
|
-
</annotation>
|
|
5325
|
-
<annotation id="G306">
|
|
5326
|
-
<infon key="type">Gene</infon>
|
|
5327
|
-
<location offset="39162" length="9"/>
|
|
5328
|
-
<text>LEU2 TRP1</text>
|
|
5329
|
-
</annotation>
|
|
5330
|
-
<annotation id="G307">
|
|
5331
|
-
<infon key="type">Gene</infon>
|
|
5332
|
-
<location offset="39173" length="14"/>
|
|
5333
|
-
<text>SPC42- GFP</text>
|
|
5334
|
-
</annotation>
|
|
5335
|
-
<annotation id="G308">
|
|
5336
|
-
<infon key="type">Gene</infon>
|
|
5337
|
-
<location offset="39198" length="93"/>
|
|
5338
|
-
<text>SPC42-GFP(3x) ura3/ura3 his3/his3 trp1-1/trp1-1 leu2-3,112/leu2-3,112 ade2-1/ade2-1 HC14-10c</text>
|
|
5339
|
-
</annotation>
|
|
5340
|
-
<annotation id="G309">
|
|
5341
|
-
<infon key="type">Gene</infon>
|
|
5342
|
-
<location offset="39307" length="23"/>
|
|
5343
|
-
<text>MATa/MATalpha ndc1Delta</text>
|
|
5344
|
-
</annotation>
|
|
5345
|
-
<annotation id="G310">
|
|
5346
|
-
<infon key="type">Gene</infon>
|
|
5347
|
-
<location offset="39312" length="18"/>
|
|
5348
|
-
<text>MATalpha ndc1Delta</text>
|
|
5349
|
-
</annotation>
|
|
5350
|
-
<annotation id="G311">
|
|
5351
|
-
<infon key="type">Gene</infon>
|
|
5352
|
-
<location offset="39349" length="150"/>
|
|
5353
|
-
<text>HIS3 ura3-52/ura3-52 his3Delta200/his3Delta200 trp1Delta63/TRP1 leu2Delta1/leu2Delta1 LYS2/ lys2-801 ade2Delta426/ade2Delta426 ade3Delta/ade3Delta</text>
|
|
5354
|
-
</annotation>
|
|
5355
|
-
<annotation id="G312">
|
|
5356
|
-
<infon key="type">Gene</infon>
|
|
5357
|
-
<location offset="39362" length="20"/>
|
|
5358
|
-
<text>ura3-52 his3Delta200</text>
|
|
5359
|
-
</annotation>
|
|
5360
|
-
<annotation id="G313">
|
|
5361
|
-
<infon key="type">Gene</infon>
|
|
5362
|
-
<location offset="39383" length="24"/>
|
|
5363
|
-
<text>his3Delta200 trp1Delta63</text>
|
|
5364
|
-
</annotation>
|
|
5365
|
-
<annotation id="G314">
|
|
5366
|
-
<infon key="type">Gene</infon>
|
|
5367
|
-
<location offset="39408" length="15"/>
|
|
5368
|
-
<text>TRP1 leu2Delta1</text>
|
|
5369
|
-
</annotation>
|
|
5370
|
-
<annotation id="G315">
|
|
5371
|
-
<infon key="type">Gene</infon>
|
|
5372
|
-
<location offset="39424" length="15"/>
|
|
5373
|
-
<text>leu2Delta1 LYS2</text>
|
|
5374
|
-
</annotation>
|
|
5375
|
-
<annotation id="G316">
|
|
5376
|
-
<infon key="type">Gene</infon>
|
|
5377
|
-
<location offset="39445" length="21"/>
|
|
5378
|
-
<text>lys2-801 ade2Delta426</text>
|
|
5379
|
-
</annotation>
|
|
5380
|
-
<annotation id="G317">
|
|
5381
|
-
<infon key="type">Gene</infon>
|
|
5382
|
-
<location offset="39467" length="22"/>
|
|
5383
|
-
<text>ade2Delta426 ade3Delta</text>
|
|
5384
|
-
</annotation>
|
|
5385
|
-
<annotation id="G318">
|
|
5386
|
-
<infon key="type">Gene</infon>
|
|
5387
|
-
<location offset="39490" length="9"/>
|
|
5388
|
-
<text>ade3Delta</text>
|
|
5389
|
-
</annotation>
|
|
5390
|
-
<annotation id="G319">
|
|
5391
|
-
<infon key="type">Gene</infon>
|
|
5392
|
-
<location offset="39501" length="15"/>
|
|
5393
|
-
<text>pRS315-NDC1-GFP</text>
|
|
5394
|
-
</annotation>
|
|
5395
|
-
<annotation id="G320">
|
|
5396
|
-
<infon key="type">Gene</infon>
|
|
5397
|
-
<location offset="39533" length="511"/>
|
|
5398
|
-
<text>MATa/MATalpha ura3-52/ura3-52 ade2/ade2 his4-539/HIS4 HC10-42a/42d MATa/MATalpha ndc1-1/ndc1-1 ura3-52/ura3-52 ade2/ade2 his4-539/HIS4 HC13-1c MATalpha NIC96-ProA-HIS3-URA3 ura3-52 his3Delta200 trp1-1 leu2-3,112 lys2-801 Wx340-3c MATalpha ndc1-1 tub2-403 ura3-52 his4-539 lys2-801 Wx340-3d MATalpha tub2-403 ura3-52 his4-539 HC14-10c MATaura3-52 his3Delta200 trp1Delta63 leu2Delta1 ade2Delta426 ade3Delta HC5-31c MATandc1-1 ura3-52 his3Delta200 trp1Delta63 leu2Delta1 ade2Delta426 ade3Delta HC5-31c</text>
|
|
5399
|
-
</annotation>
|
|
5400
|
-
<annotation id="G321">
|
|
5401
|
-
<infon key="type">Gene</infon>
|
|
5402
|
-
<location offset="39538" length="16"/>
|
|
5403
|
-
<text>MATalpha ura3-52</text>
|
|
5404
|
-
</annotation>
|
|
5405
|
-
<annotation id="G322">
|
|
5406
|
-
<infon key="type">Gene</infon>
|
|
5407
|
-
<location offset="39555" length="12"/>
|
|
5408
|
-
<text>ura3-52 ade2</text>
|
|
5409
|
-
</annotation>
|
|
5410
|
-
<annotation id="G323">
|
|
5411
|
-
<infon key="type">Gene</infon>
|
|
5412
|
-
<location offset="39568" length="13"/>
|
|
5413
|
-
<text>ade2 his4-539</text>
|
|
5414
|
-
</annotation>
|
|
5415
|
-
<annotation id="G324">
|
|
5416
|
-
<infon key="type">Gene</infon>
|
|
5417
|
-
<location offset="39582" length="14"/>
|
|
5418
|
-
<text>HIS4 HC10-42a</text>
|
|
5419
|
-
</annotation>
|
|
5420
|
-
<annotation id="G325">
|
|
5421
|
-
<infon key="type">Gene</infon>
|
|
5422
|
-
<location offset="39597" length="9"/>
|
|
5423
|
-
<text>42d MATa</text>
|
|
5424
|
-
</annotation>
|
|
5425
|
-
<annotation id="G326">
|
|
5426
|
-
<infon key="type">Gene</infon>
|
|
5427
|
-
<location offset="39607" length="15"/>
|
|
5428
|
-
<text>MATalpha ndc1-1</text>
|
|
5429
|
-
</annotation>
|
|
5430
|
-
<annotation id="G327">
|
|
5431
|
-
<infon key="type">Gene</infon>
|
|
5432
|
-
<location offset="39623" length="14"/>
|
|
5433
|
-
<text>ndc1-1 ura3-52</text>
|
|
5434
|
-
</annotation>
|
|
5435
|
-
<annotation id="G328">
|
|
5436
|
-
<infon key="type">Gene</infon>
|
|
5437
|
-
<location offset="39638" length="12"/>
|
|
5438
|
-
<text>ura3-52 ade2</text>
|
|
5439
|
-
</annotation>
|
|
5440
|
-
<annotation id="G329">
|
|
5441
|
-
<infon key="type">Gene</infon>
|
|
5442
|
-
<location offset="39651" length="13"/>
|
|
5443
|
-
<text>ade2 his4-539</text>
|
|
5444
|
-
</annotation>
|
|
5445
|
-
<annotation id="G330">
|
|
5446
|
-
<infon key="type">Gene</infon>
|
|
5447
|
-
<location offset="39665" length="379"/>
|
|
5448
|
-
<text>HIS4 HC13-1c MATalpha NIC96-ProA-HIS3-URA3 ura3-52 his3Delta200 trp1-1 leu2-3,112 lys2-801 Wx340-3c MATalpha ndc1-1 tub2-403 ura3-52 his4-539 lys2-801 Wx340-3d MATalpha tub2-403 ura3-52 his4-539 HC14-10c MATaura3-52 his3Delta200 trp1Delta63 leu2Delta1 ade2Delta426 ade3Delta HC5-31c MATandc1-1 ura3-52 his3Delta200 trp1Delta63 leu2Delta1 ade2Delta426 ade3Delta HC5-31c</text>
|
|
5449
|
-
</annotation>
|
|
5450
|
-
<annotation id="G331">
|
|
5451
|
-
<infon key="type">Gene</infon>
|
|
5452
|
-
<location offset="40052" length="22"/>
|
|
5453
|
-
<text>MATandc1-1 pom152Delta</text>
|
|
5454
|
-
</annotation>
|
|
5455
|
-
<annotation id="G332">
|
|
5456
|
-
<infon key="type">Gene</infon>
|
|
5457
|
-
<location offset="40076" length="71"/>
|
|
5458
|
-
<text>HIS3 ura3-52 his3Delta200 trp1Delta63 leu2Delta1 ade2Delta426 ade3Delta</text>
|
|
5459
|
-
</annotation>
|
|
5460
|
-
<annotation id="TEAM_277_94">
|
|
5461
|
-
<infon key="type">Gene</infon>
|
|
5462
|
-
<location offset="38160" length="307"/>
|
|
5463
|
-
<text>MATalpha NDC1-ProA-HIS3-URA3/NDC1-ProA-HIS3-URA3 ura3-52/ura3-52 his3Delta200/his3Delta200 trp1-1/trp1-1 leu2-3,112/leu2-3,112 lys2-801/lys2-801 ProA5-4d MATaNDC1-ProA-HIS3-URA3 ura3-52 his3Delta200 trp1-1 leu2-3,112 lys2-801 HC26-15a/1a MATa/MATalpha NDC1-ProA-HIS3-URA3/NDC1-ProA-HIS3-URA3 nup49-1</text>
|
|
5464
|
-
</annotation>
|
|
5465
|
-
<annotation id="TEAM_277_95">
|
|
5466
|
-
<infon key="type">Gene</infon>
|
|
5467
|
-
<infon key="GeneID">13745</infon>
|
|
5468
|
-
<location offset="38469" length="4"/>
|
|
5469
|
-
<text>URA3</text>
|
|
5470
|
-
</annotation>
|
|
5471
|
-
<annotation id="TEAM_277_96">
|
|
5472
|
-
<infon key="type">Gene</infon>
|
|
5473
|
-
<location offset="38539" length="28"/>
|
|
5474
|
-
<text>GFP-S65T-TRP1 ura3/ura3 his3</text>
|
|
5475
|
-
</annotation>
|
|
5476
|
-
<annotation id="TEAM_277_97">
|
|
5477
|
-
<infon key="type">Gene</infon>
|
|
5478
|
-
<location offset="38568" length="81"/>
|
|
5479
|
-
<text>his3 trp1-1/trp1-1 leu2-3,112/leu2-3,112 HC27-16d/16b MATa/MATalpha nup133Delta</text>
|
|
5480
|
-
</annotation>
|
|
5481
|
-
<annotation id="TEAM_277_98">
|
|
5482
|
-
<infon key="type">Gene</infon>
|
|
5483
|
-
<infon key="GeneID">393202</infon>
|
|
5484
|
-
<location offset="38651" length="4"/>
|
|
5485
|
-
<text>HIS3</text>
|
|
5486
|
-
</annotation>
|
|
5487
|
-
<annotation id="TEAM_277_99">
|
|
5488
|
-
<infon key="type">Gene</infon>
|
|
5489
|
-
<infon key="GeneID">393202</infon>
|
|
5490
|
-
<location offset="38669" length="4"/>
|
|
5491
|
-
<text>HIS3</text>
|
|
5492
|
-
</annotation>
|
|
5493
|
-
<annotation id="TEAM_277_100">
|
|
5494
|
-
<infon key="type">Gene</infon>
|
|
5495
|
-
<location offset="38694" length="4"/>
|
|
5496
|
-
<text>NDC1</text>
|
|
5497
|
-
</annotation>
|
|
5498
|
-
<annotation id="TEAM_277_101">
|
|
5499
|
-
<infon key="type">Gene</infon>
|
|
5500
|
-
<infon key="GeneID">13745</infon>
|
|
5501
|
-
<location offset="38708" length="4"/>
|
|
5502
|
-
<text>URA3</text>
|
|
5503
|
-
</annotation>
|
|
5504
|
-
<annotation id="TEAM_277_102">
|
|
5505
|
-
<infon key="type">Gene</infon>
|
|
5506
|
-
<location offset="38777" length="28"/>
|
|
5507
|
-
<text>GFP-S65T-TRP1 ura3/ura3 his3</text>
|
|
5508
|
-
</annotation>
|
|
5509
|
-
<annotation id="TEAM_277_103">
|
|
5510
|
-
<infon key="type">Gene</infon>
|
|
5511
|
-
<location offset="38806" length="87"/>
|
|
5512
|
-
<text>his3 trp1-1/trp1-1 leu2-3,112/leu2-3,112 HC22-2b/1c MATa/MATalpha NDC1-ProA-HIS3-URA3</text>
|
|
5513
|
-
</annotation>
|
|
5514
|
-
<annotation id="TEAM_277_104">
|
|
5515
|
-
<infon key="type">Gene</infon>
|
|
5516
|
-
<infon key="GeneID">9980</infon>
|
|
5517
|
-
<location offset="38926" length="4"/>
|
|
5518
|
-
<text>LEU2</text>
|
|
5519
|
-
</annotation>
|
|
5520
|
-
<annotation id="TEAM_277_105">
|
|
5521
|
-
<infon key="type">Gene</infon>
|
|
5522
|
-
<infon key="GeneID">9980</infon>
|
|
5523
|
-
<location offset="38943" length="4"/>
|
|
5524
|
-
<text>LEU2</text>
|
|
5525
|
-
</annotation>
|
|
5526
|
-
<annotation id="TEAM_277_106">
|
|
5527
|
-
<infon key="type">Gene</infon>
|
|
5528
|
-
<location offset="38952" length="4"/>
|
|
5529
|
-
<text>TRP1</text>
|
|
5530
|
-
</annotation>
|
|
5531
|
-
<annotation id="TEAM_277_107">
|
|
5532
|
-
<infon key="type">Gene</infon>
|
|
5533
|
-
<location offset="38972" length="4"/>
|
|
5534
|
-
<text>TRP1</text>
|
|
5535
|
-
</annotation>
|
|
5536
|
-
<annotation id="TEAM_277_108">
|
|
5537
|
-
<infon key="type">Gene</infon>
|
|
5538
|
-
<location offset="38978" length="28"/>
|
|
5539
|
-
<text>SPC42-GFP(3x) ura3/ura3 his3</text>
|
|
5540
|
-
</annotation>
|
|
5541
|
-
<annotation id="TEAM_277_109">
|
|
5542
|
-
<infon key="type">Gene</infon>
|
|
5543
|
-
<location offset="39007" length="104"/>
|
|
5544
|
-
<text>his3 trp1-1/trp1-1 leu2-3,112/leu2-3,112 ade2-1/ade2-1 HC23-11d/16a MATa/MATalpha NIC96-ProA-HIS3-URA3</text>
|
|
5545
|
-
</annotation>
|
|
5546
|
-
<annotation id="TEAM_277_110">
|
|
5547
|
-
<infon key="type">Gene</infon>
|
|
5548
|
-
<infon key="GeneID">9980</infon>
|
|
5549
|
-
<location offset="39145" length="4"/>
|
|
5550
|
-
<text>LEU2</text>
|
|
5551
|
-
</annotation>
|
|
5552
|
-
<annotation id="TEAM_277_111">
|
|
5553
|
-
<infon key="type">Gene</infon>
|
|
5554
|
-
<infon key="GeneID">9980</infon>
|
|
5555
|
-
<location offset="39162" length="4"/>
|
|
5556
|
-
<text>LEU2</text>
|
|
5557
|
-
</annotation>
|
|
5558
|
-
<annotation id="TEAM_277_112">
|
|
5559
|
-
<infon key="type">Gene</infon>
|
|
5560
|
-
<location offset="39167" length="4"/>
|
|
5561
|
-
<text>TRP1</text>
|
|
5562
|
-
</annotation>
|
|
5563
|
-
<annotation id="TEAM_277_113">
|
|
5564
|
-
<infon key="type">Gene</infon>
|
|
5565
|
-
<location offset="39173" length="23"/>
|
|
5566
|
-
<text>SPC42- GFP(3x)/TRP1</text>
|
|
5567
|
-
</annotation>
|
|
5568
|
-
<annotation id="TEAM_277_114">
|
|
5569
|
-
<infon key="type">Gene</infon>
|
|
5570
|
-
<location offset="39198" length="28"/>
|
|
5571
|
-
<text>SPC42-GFP(3x) ura3/ura3 his3</text>
|
|
5572
|
-
</annotation>
|
|
5573
|
-
<annotation id="TEAM_277_115">
|
|
5574
|
-
<infon key="type">Gene</infon>
|
|
5575
|
-
<location offset="39227" length="84"/>
|
|
5576
|
-
<text>his3 trp1-1/trp1-1 leu2-3,112/leu2-3,112 ade2-1/ade2-1 HC14-10c(1198)/HC29-6b MATa</text>
|
|
2920
|
+
<infon key="type">DNAMutation</infon>
|
|
2921
|
+
<location offset="39428" length="6"/>
|
|
2922
|
+
<text>Delta1</text>
|
|
5577
2923
|
</annotation>
|
|
5578
|
-
<annotation id="
|
|
5579
|
-
<infon key="
|
|
5580
|
-
<infon key="
|
|
5581
|
-
<location offset="
|
|
5582
|
-
<text>
|
|
2924
|
+
<annotation id="C553">
|
|
2925
|
+
<infon key="tmVar">|DEL||1</infon>
|
|
2926
|
+
<infon key="type">DNAMutation</infon>
|
|
2927
|
+
<location offset="39417" length="6"/>
|
|
2928
|
+
<text>Delta1</text>
|
|
5583
2929
|
</annotation>
|
|
5584
|
-
<annotation id="
|
|
5585
|
-
<infon key="
|
|
5586
|
-
<
|
|
5587
|
-
<
|
|
2930
|
+
<annotation id="C554">
|
|
2931
|
+
<infon key="tmVar">p|SUB|S|65|T</infon>
|
|
2932
|
+
<infon key="type">ProteinMutation</infon>
|
|
2933
|
+
<location offset="38781" length="4"/>
|
|
2934
|
+
<text>S65T</text>
|
|
5588
2935
|
</annotation>
|
|
5589
|
-
<annotation id="
|
|
5590
|
-
<infon key="
|
|
5591
|
-
<
|
|
5592
|
-
<
|
|
2936
|
+
<annotation id="C555">
|
|
2937
|
+
<infon key="tmVar">p|SUB|S|65|T</infon>
|
|
2938
|
+
<infon key="type">ProteinMutation</infon>
|
|
2939
|
+
<location offset="38751" length="4"/>
|
|
2940
|
+
<text>S65T</text>
|
|
5593
2941
|
</annotation>
|
|
5594
|
-
<annotation id="
|
|
5595
|
-
<infon key="
|
|
5596
|
-
<
|
|
5597
|
-
<
|
|
2942
|
+
<annotation id="C556">
|
|
2943
|
+
<infon key="tmVar">p|SUB|S|65|T</infon>
|
|
2944
|
+
<infon key="type">ProteinMutation</infon>
|
|
2945
|
+
<location offset="38543" length="4"/>
|
|
2946
|
+
<text>S65T</text>
|
|
5598
2947
|
</annotation>
|
|
5599
|
-
<annotation id="
|
|
5600
|
-
<infon key="
|
|
5601
|
-
<
|
|
5602
|
-
<
|
|
2948
|
+
<annotation id="C557">
|
|
2949
|
+
<infon key="tmVar">p|SUB|S|65|T</infon>
|
|
2950
|
+
<infon key="type">ProteinMutation</infon>
|
|
2951
|
+
<location offset="38513" length="4"/>
|
|
2952
|
+
<text>S65T</text>
|
|
5603
2953
|
</annotation>
|
|
5604
2954
|
</passage>
|
|
5605
2955
|
<passage>
|
|
@@ -5659,68 +3009,6 @@
|
|
|
5659
3009
|
<location offset="41093" length="3"/>
|
|
5660
3010
|
<text>GFP</text>
|
|
5661
3011
|
</annotation>
|
|
5662
|
-
<annotation id="G333">
|
|
5663
|
-
<infon key="type">Gene</infon>
|
|
5664
|
-
<location offset="40065" length="10"/>
|
|
5665
|
-
<text>Ndc1p-ProA</text>
|
|
5666
|
-
</annotation>
|
|
5667
|
-
<annotation id="G334">
|
|
5668
|
-
<infon key="type">Gene</infon>
|
|
5669
|
-
<location offset="40454" length="10"/>
|
|
5670
|
-
<text>Ndc1p-ProA</text>
|
|
5671
|
-
</annotation>
|
|
5672
|
-
<annotation id="G335">
|
|
5673
|
-
<infon key="score">0.73</infon>
|
|
5674
|
-
<infon key="GeneID">852703</infon>
|
|
5675
|
-
<infon key="type">Gene</infon>
|
|
5676
|
-
<location offset="40469" length="10"/>
|
|
5677
|
-
<text>Nup49p-GFP</text>
|
|
5678
|
-
</annotation>
|
|
5679
|
-
<annotation id="G336">
|
|
5680
|
-
<infon key="score">0.73</infon>
|
|
5681
|
-
<infon key="GeneID">852703</infon>
|
|
5682
|
-
<infon key="type">Gene</infon>
|
|
5683
|
-
<location offset="40596" length="10"/>
|
|
5684
|
-
<text>Nup49p-GFP</text>
|
|
5685
|
-
</annotation>
|
|
5686
|
-
<annotation id="G337">
|
|
5687
|
-
<infon key="score">0.73</infon>
|
|
5688
|
-
<infon key="GeneID">7011691</infon>
|
|
5689
|
-
<infon key="type">Gene</infon>
|
|
5690
|
-
<location offset="40710" length="3"/>
|
|
5691
|
-
<text>GFP</text>
|
|
5692
|
-
</annotation>
|
|
5693
|
-
<annotation id="G338">
|
|
5694
|
-
<infon key="type">Gene</infon>
|
|
5695
|
-
<location offset="40795" length="10"/>
|
|
5696
|
-
<text>Ndc1p-ProA</text>
|
|
5697
|
-
</annotation>
|
|
5698
|
-
<annotation id="G339">
|
|
5699
|
-
<infon key="score">0.73</infon>
|
|
5700
|
-
<infon key="GeneID">852703</infon>
|
|
5701
|
-
<infon key="type">Gene</infon>
|
|
5702
|
-
<location offset="40823" length="10"/>
|
|
5703
|
-
<text>Nup49p-GFP</text>
|
|
5704
|
-
</annotation>
|
|
5705
|
-
<annotation id="G340">
|
|
5706
|
-
<infon key="type">Gene</infon>
|
|
5707
|
-
<location offset="40871" length="18"/>
|
|
5708
|
-
<text>nup133 null allele</text>
|
|
5709
|
-
</annotation>
|
|
5710
|
-
<annotation id="G341">
|
|
5711
|
-
<infon key="score">0.73</infon>
|
|
5712
|
-
<infon key="GeneID">852703</infon>
|
|
5713
|
-
<infon key="type">Gene</infon>
|
|
5714
|
-
<location offset="41007" length="10"/>
|
|
5715
|
-
<text>Nup49p-GFP</text>
|
|
5716
|
-
</annotation>
|
|
5717
|
-
<annotation id="G342">
|
|
5718
|
-
<infon key="score">0.73</infon>
|
|
5719
|
-
<infon key="GeneID">7011691</infon>
|
|
5720
|
-
<infon key="type">Gene</infon>
|
|
5721
|
-
<location offset="41093" length="3"/>
|
|
5722
|
-
<text>GFP</text>
|
|
5723
|
-
</annotation>
|
|
5724
3012
|
</passage>
|
|
5725
3013
|
<passage>
|
|
5726
3014
|
<infon key="type">fig_caption</infon>
|
|
@@ -5804,79 +3092,6 @@
|
|
|
5804
3092
|
<location offset="41579" length="134"/>
|
|
5805
3093
|
<text>Pom152p is a NPC component (Wozniak et al., 1994), and Spc110p is a SPB component (Rout and Kilmartin, 1990; Kilmartin et al., 1993)</text>
|
|
5806
3094
|
</annotation>
|
|
5807
|
-
<annotation id="G343">
|
|
5808
|
-
<infon key="type">Gene</infon>
|
|
5809
|
-
<location offset="41161" length="10"/>
|
|
5810
|
-
<text>Ndc1p-ProA</text>
|
|
5811
|
-
</annotation>
|
|
5812
|
-
<annotation id="G344">
|
|
5813
|
-
<infon key="type">Gene</infon>
|
|
5814
|
-
<location offset="41297" length="9"/>
|
|
5815
|
-
<text>Kilmartin</text>
|
|
5816
|
-
</annotation>
|
|
5817
|
-
<annotation id="G345">
|
|
5818
|
-
<infon key="type">Gene</infon>
|
|
5819
|
-
<location offset="41324" length="9"/>
|
|
5820
|
-
<text>Kilmartin</text>
|
|
5821
|
-
</annotation>
|
|
5822
|
-
<annotation id="G346">
|
|
5823
|
-
<infon key="score">0.73</infon>
|
|
5824
|
-
<infon key="GeneID">855159</infon>
|
|
5825
|
-
<infon key="type">Gene</infon>
|
|
5826
|
-
<location offset="41546" length="7"/>
|
|
5827
|
-
<text>Pom152p</text>
|
|
5828
|
-
</annotation>
|
|
5829
|
-
<annotation id="G347">
|
|
5830
|
-
<infon key="score">0.73</infon>
|
|
5831
|
-
<infon key="GeneID">851957</infon>
|
|
5832
|
-
<infon key="type">Gene</infon>
|
|
5833
|
-
<location offset="41570" length="7"/>
|
|
5834
|
-
<text>Spc110p</text>
|
|
5835
|
-
</annotation>
|
|
5836
|
-
<annotation id="G348">
|
|
5837
|
-
<infon key="score">0.73</infon>
|
|
5838
|
-
<infon key="GeneID">855159</infon>
|
|
5839
|
-
<infon key="type">Gene</infon>
|
|
5840
|
-
<location offset="41579" length="7"/>
|
|
5841
|
-
<text>Pom152p</text>
|
|
5842
|
-
</annotation>
|
|
5843
|
-
<annotation id="G349">
|
|
5844
|
-
<infon key="score">0.73</infon>
|
|
5845
|
-
<infon key="GeneID">851957</infon>
|
|
5846
|
-
<infon key="type">Gene</infon>
|
|
5847
|
-
<location offset="41635" length="7"/>
|
|
5848
|
-
<text>Spc110p</text>
|
|
5849
|
-
</annotation>
|
|
5850
|
-
<annotation id="G350">
|
|
5851
|
-
<infon key="type">Gene</infon>
|
|
5852
|
-
<location offset="41672" length="9"/>
|
|
5853
|
-
<text>Kilmartin</text>
|
|
5854
|
-
</annotation>
|
|
5855
|
-
<annotation id="G351">
|
|
5856
|
-
<infon key="type">Gene</infon>
|
|
5857
|
-
<location offset="42156" length="10"/>
|
|
5858
|
-
<text>Ndc1p-ProA</text>
|
|
5859
|
-
</annotation>
|
|
5860
|
-
<annotation id="G352">
|
|
5861
|
-
<infon key="score">0.73</infon>
|
|
5862
|
-
<infon key="GeneID">855159</infon>
|
|
5863
|
-
<infon key="type">Gene</infon>
|
|
5864
|
-
<location offset="42363" length="7"/>
|
|
5865
|
-
<text>Pom152p</text>
|
|
5866
|
-
</annotation>
|
|
5867
|
-
<annotation id="G353">
|
|
5868
|
-
<infon key="score">0.73</infon>
|
|
5869
|
-
<infon key="GeneID">853016</infon>
|
|
5870
|
-
<infon key="type">Gene</infon>
|
|
5871
|
-
<location offset="42376" length="6"/>
|
|
5872
|
-
<text>Nup57p</text>
|
|
5873
|
-
</annotation>
|
|
5874
|
-
<annotation id="TEAM_277_121">
|
|
5875
|
-
<infon key="type">Gene</infon>
|
|
5876
|
-
<infon key="GeneID">4415</infon>
|
|
5877
|
-
<location offset="42376" length="6"/>
|
|
5878
|
-
<text>Nup57p</text>
|
|
5879
|
-
</annotation>
|
|
5880
3095
|
</passage>
|
|
5881
3096
|
<passage>
|
|
5882
3097
|
<infon key="type">fig_caption</infon>
|
|
@@ -5924,70 +3139,6 @@
|
|
|
5924
3139
|
<location offset="43188" length="6"/>
|
|
5925
3140
|
<text>nup133</text>
|
|
5926
3141
|
</annotation>
|
|
5927
|
-
<annotation id="G354">
|
|
5928
|
-
<infon key="type">Gene</infon>
|
|
5929
|
-
<location offset="42384" length="10"/>
|
|
5930
|
-
<text>Ndc1p-ProA</text>
|
|
5931
|
-
</annotation>
|
|
5932
|
-
<annotation id="G355">
|
|
5933
|
-
<infon key="type">Gene</infon>
|
|
5934
|
-
<location offset="42514" length="10"/>
|
|
5935
|
-
<text>Ndc1p-ProA</text>
|
|
5936
|
-
</annotation>
|
|
5937
|
-
<annotation id="G356">
|
|
5938
|
-
<infon key="score">0.73</infon>
|
|
5939
|
-
<infon key="GeneID">853824</infon>
|
|
5940
|
-
<infon key="type">Gene</infon>
|
|
5941
|
-
<location offset="42530" length="10"/>
|
|
5942
|
-
<text>Spc42p-GFP</text>
|
|
5943
|
-
</annotation>
|
|
5944
|
-
<annotation id="G357">
|
|
5945
|
-
<infon key="score">0.73</infon>
|
|
5946
|
-
<infon key="GeneID">853824</infon>
|
|
5947
|
-
<infon key="type">Gene</infon>
|
|
5948
|
-
<location offset="42622" length="10"/>
|
|
5949
|
-
<text>Spc42p-GFP</text>
|
|
5950
|
-
</annotation>
|
|
5951
|
-
<annotation id="G358">
|
|
5952
|
-
<infon key="type">Gene</infon>
|
|
5953
|
-
<location offset="42886" length="11"/>
|
|
5954
|
-
<text>Nic96p-ProA</text>
|
|
5955
|
-
</annotation>
|
|
5956
|
-
<annotation id="G359">
|
|
5957
|
-
<infon key="score">0.73</infon>
|
|
5958
|
-
<infon key="GeneID">853824</infon>
|
|
5959
|
-
<infon key="type">Gene</infon>
|
|
5960
|
-
<location offset="42902" length="10"/>
|
|
5961
|
-
<text>Spc42p-GFP</text>
|
|
5962
|
-
</annotation>
|
|
5963
|
-
<annotation id="G360">
|
|
5964
|
-
<infon key="score">0.73</infon>
|
|
5965
|
-
<infon key="GeneID">852703</infon>
|
|
5966
|
-
<infon key="type">Gene</infon>
|
|
5967
|
-
<location offset="43172" length="10"/>
|
|
5968
|
-
<text>Nup49p-GFP</text>
|
|
5969
|
-
</annotation>
|
|
5970
|
-
<annotation id="G361">
|
|
5971
|
-
<infon key="score">0.70</infon>
|
|
5972
|
-
<infon key="GeneID">406852</infon>
|
|
5973
|
-
<infon key="type">Gene</infon>
|
|
5974
|
-
<location offset="43188" length="6"/>
|
|
5975
|
-
<text>nup133</text>
|
|
5976
|
-
</annotation>
|
|
5977
|
-
<annotation id="G362">
|
|
5978
|
-
<infon key="score">0.73</infon>
|
|
5979
|
-
<infon key="GeneID">852703</infon>
|
|
5980
|
-
<infon key="type">Gene</infon>
|
|
5981
|
-
<location offset="43302" length="10"/>
|
|
5982
|
-
<text>Nup49p-GFP</text>
|
|
5983
|
-
</annotation>
|
|
5984
|
-
<annotation id="G363">
|
|
5985
|
-
<infon key="score">0.73</infon>
|
|
5986
|
-
<infon key="GeneID">852703</infon>
|
|
5987
|
-
<infon key="type">Gene</infon>
|
|
5988
|
-
<location offset="43571" length="10"/>
|
|
5989
|
-
<text>Nup49p-GFP</text>
|
|
5990
|
-
</annotation>
|
|
5991
3142
|
</passage>
|
|
5992
3143
|
<passage>
|
|
5993
3144
|
<infon key="type">fig_caption</infon>
|
|
@@ -6011,53 +3162,6 @@
|
|
|
6011
3162
|
<location offset="43835" length="3"/>
|
|
6012
3163
|
<text>GFP</text>
|
|
6013
3164
|
</annotation>
|
|
6014
|
-
<annotation id="G364">
|
|
6015
|
-
<infon key="score">0.73</infon>
|
|
6016
|
-
<infon key="GeneID">854977</infon>
|
|
6017
|
-
<infon key="type">Gene</infon>
|
|
6018
|
-
<location offset="43592" length="9"/>
|
|
6019
|
-
<text>Ndc1p-GFP</text>
|
|
6020
|
-
</annotation>
|
|
6021
|
-
<annotation id="G365">
|
|
6022
|
-
<infon key="type">Gene</infon>
|
|
6023
|
-
<location offset="43754" length="16"/>
|
|
6024
|
-
<text>ndc1 null allele</text>
|
|
6025
|
-
</annotation>
|
|
6026
|
-
<annotation id="G366">
|
|
6027
|
-
<infon key="score">0.70</infon>
|
|
6028
|
-
<infon key="GeneID">335236</infon>
|
|
6029
|
-
<infon key="type">Gene</infon>
|
|
6030
|
-
<location offset="43830" length="8"/>
|
|
6031
|
-
<text>NDC1-GFP</text>
|
|
6032
|
-
</annotation>
|
|
6033
|
-
<annotation id="G367">
|
|
6034
|
-
<infon key="score">0.73</infon>
|
|
6035
|
-
<infon key="GeneID">854977</infon>
|
|
6036
|
-
<infon key="type">Gene</infon>
|
|
6037
|
-
<location offset="44143" length="9"/>
|
|
6038
|
-
<text>Ndc1p-GFP</text>
|
|
6039
|
-
</annotation>
|
|
6040
|
-
<annotation id="TEAM_277_122">
|
|
6041
|
-
<infon key="type">Gene</infon>
|
|
6042
|
-
<location offset="43667" length="9"/>
|
|
6043
|
-
<text>Ndc1p-GFP</text>
|
|
6044
|
-
</annotation>
|
|
6045
|
-
<annotation id="TEAM_277_123">
|
|
6046
|
-
<infon key="type">Gene</infon>
|
|
6047
|
-
<location offset="43877" length="9"/>
|
|
6048
|
-
<text>Ndc1p-GFP</text>
|
|
6049
|
-
</annotation>
|
|
6050
|
-
<annotation id="TEAM_277_124">
|
|
6051
|
-
<infon key="type">Gene</infon>
|
|
6052
|
-
<location offset="44143" length="9"/>
|
|
6053
|
-
<text>Ndc1p-GFP</text>
|
|
6054
|
-
</annotation>
|
|
6055
|
-
<annotation id="5">
|
|
6056
|
-
<infon key="type">ExperimentalMethod</infon>
|
|
6057
|
-
<infon key="PSIMI">0416</infon>
|
|
6058
|
-
<location offset="43877" length="226"/>
|
|
6059
|
-
<text>Ndc1p-GFP localization was observed in living cells using deconvolution fluorescence microscopy (A). Additionally, this localization was examined using immunoelectron microscopy of whole cells (see Materials and Methods).</text>
|
|
6060
|
-
</annotation>
|
|
6061
3165
|
</passage>
|
|
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|
<passage>
|
|
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|
<infon key="type">fig_caption</infon>
|
|
@@ -6101,38 +3205,6 @@
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|
|
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3205
|
<location offset="45043" length="4"/>
|
|
6102
3206
|
<text>FITC</text>
|
|
6103
3207
|
</annotation>
|
|
6104
|
-
<annotation id="G368">
|
|
6105
|
-
<infon key="type">Gene</infon>
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|
6106
|
-
<location offset="44326" length="6"/>
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|
6107
|
-
<text>ndc1-1</text>
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|
6108
|
-
</annotation>
|
|
6109
|
-
<annotation id="G369">
|
|
6110
|
-
<infon key="type">Gene</infon>
|
|
6111
|
-
<location offset="44495" length="12"/>
|
|
6112
|
-
<text>HC10-42b/42c</text>
|
|
6113
|
-
</annotation>
|
|
6114
|
-
<annotation id="G370">
|
|
6115
|
-
<infon key="score">0.70</infon>
|
|
6116
|
-
<infon key="GeneID">20194</infon>
|
|
6117
|
-
<infon key="type">Gene</infon>
|
|
6118
|
-
<location offset="44504" length="3"/>
|
|
6119
|
-
<text>42c</text>
|
|
6120
|
-
</annotation>
|
|
6121
|
-
<annotation id="G371">
|
|
6122
|
-
<infon key="type">Gene</infon>
|
|
6123
|
-
<location offset="44571" length="10"/>
|
|
6124
|
-
<text>NIC96-ProA</text>
|
|
6125
|
-
</annotation>
|
|
6126
|
-
<annotation id="G372">
|
|
6127
|
-
<infon key="type">Gene</infon>
|
|
6128
|
-
<location offset="44647" length="15"/>
|
|
6129
|
-
<text>pGAL-NIC96-ProA</text>
|
|
6130
|
-
</annotation>
|
|
6131
|
-
<annotation id="G373">
|
|
6132
|
-
<infon key="type">Gene</infon>
|
|
6133
|
-
<location offset="44749" length="14"/>
|
|
6134
|
-
<text>ndc1-1 culture</text>
|
|
6135
|
-
</annotation>
|
|
6136
3208
|
</passage>
|
|
6137
3209
|
<passage>
|
|
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|
<infon key="type">fig_caption</infon>
|
|
@@ -6245,129 +3317,6 @@
|
|
|
6245
3317
|
<location offset="45954" length="6"/>
|
|
6246
3318
|
<text>pom152</text>
|
|
6247
3319
|
</annotation>
|
|
6248
|
-
<annotation id="G374">
|
|
6249
|
-
<infon key="score">0.70</infon>
|
|
6250
|
-
<infon key="GeneID">855159</infon>
|
|
6251
|
-
<infon key="type">Gene</infon>
|
|
6252
|
-
<location offset="45121" length="6"/>
|
|
6253
|
-
<text>POM152</text>
|
|
6254
|
-
</annotation>
|
|
6255
|
-
<annotation id="G375">
|
|
6256
|
-
<infon key="type">Gene</infon>
|
|
6257
|
-
<location offset="45143" length="10"/>
|
|
6258
|
-
<text>ndc1-1 SPB</text>
|
|
6259
|
-
</annotation>
|
|
6260
|
-
<annotation id="G376">
|
|
6261
|
-
<infon key="type">Gene</infon>
|
|
6262
|
-
<location offset="45202" length="6"/>
|
|
6263
|
-
<text>ndc1-1</text>
|
|
6264
|
-
</annotation>
|
|
6265
|
-
<annotation id="G377">
|
|
6266
|
-
<infon key="type">Gene</infon>
|
|
6267
|
-
<location offset="45212" length="6"/>
|
|
6268
|
-
<text>ndc1-1</text>
|
|
6269
|
-
</annotation>
|
|
6270
|
-
<annotation id="G378">
|
|
6271
|
-
<infon key="score">0.70</infon>
|
|
6272
|
-
<infon key="GeneID">855159</infon>
|
|
6273
|
-
<infon key="type">Gene</infon>
|
|
6274
|
-
<location offset="45220" length="6"/>
|
|
6275
|
-
<text>pom152</text>
|
|
6276
|
-
</annotation>
|
|
6277
|
-
<annotation id="G379">
|
|
6278
|
-
<infon key="type">Gene</infon>
|
|
6279
|
-
<location offset="45317" length="10"/>
|
|
6280
|
-
<text>pURA3-NDC1</text>
|
|
6281
|
-
</annotation>
|
|
6282
|
-
<annotation id="G380">
|
|
6283
|
-
<infon key="type">Gene</infon>
|
|
6284
|
-
<location offset="45381" length="10"/>
|
|
6285
|
-
<text>pURA3-NDC1</text>
|
|
6286
|
-
</annotation>
|
|
6287
|
-
<annotation id="G381">
|
|
6288
|
-
<infon key="type">Gene</infon>
|
|
6289
|
-
<location offset="45429" length="5"/>
|
|
6290
|
-
<text>pLEU2</text>
|
|
6291
|
-
</annotation>
|
|
6292
|
-
<annotation id="G382">
|
|
6293
|
-
<infon key="type">Gene</infon>
|
|
6294
|
-
<location offset="45439" length="7"/>
|
|
6295
|
-
<text>pPM1-HA</text>
|
|
6296
|
-
</annotation>
|
|
6297
|
-
<annotation id="G383">
|
|
6298
|
-
<infon key="type">Gene</infon>
|
|
6299
|
-
<location offset="45593" length="6"/>
|
|
6300
|
-
<text>ndc1-1</text>
|
|
6301
|
-
</annotation>
|
|
6302
|
-
<annotation id="G384">
|
|
6303
|
-
<infon key="type">Gene</infon>
|
|
6304
|
-
<location offset="45615" length="10"/>
|
|
6305
|
-
<text>pURA3-NDC1</text>
|
|
6306
|
-
</annotation>
|
|
6307
|
-
<annotation id="G385">
|
|
6308
|
-
<infon key="type">Gene</infon>
|
|
6309
|
-
<location offset="45656" length="6"/>
|
|
6310
|
-
<text>ndc1-1</text>
|
|
6311
|
-
</annotation>
|
|
6312
|
-
<annotation id="G386">
|
|
6313
|
-
<infon key="score">0.70</infon>
|
|
6314
|
-
<infon key="GeneID">855159</infon>
|
|
6315
|
-
<infon key="type">Gene</infon>
|
|
6316
|
-
<location offset="45664" length="6"/>
|
|
6317
|
-
<text>pom152</text>
|
|
6318
|
-
</annotation>
|
|
6319
|
-
<annotation id="G387">
|
|
6320
|
-
<infon key="type">Gene</infon>
|
|
6321
|
-
<location offset="45860" length="6"/>
|
|
6322
|
-
<text>ndc1-1</text>
|
|
6323
|
-
</annotation>
|
|
6324
|
-
<annotation id="G388">
|
|
6325
|
-
<infon key="type">Gene</infon>
|
|
6326
|
-
<location offset="45933" length="6"/>
|
|
6327
|
-
<text>ndc1-1</text>
|
|
6328
|
-
</annotation>
|
|
6329
|
-
<annotation id="G389">
|
|
6330
|
-
<infon key="type">Gene</infon>
|
|
6331
|
-
<location offset="45946" length="6"/>
|
|
6332
|
-
<text>ndc1-1</text>
|
|
6333
|
-
</annotation>
|
|
6334
|
-
<annotation id="G390">
|
|
6335
|
-
<infon key="type">Gene</infon>
|
|
6336
|
-
<location offset="45981" length="6"/>
|
|
6337
|
-
<text>ndc1-1</text>
|
|
6338
|
-
</annotation>
|
|
6339
|
-
<annotation id="G391">
|
|
6340
|
-
<infon key="type">Gene</infon>
|
|
6341
|
-
<location offset="45989" length="11"/>
|
|
6342
|
-
<text>pom152Delta</text>
|
|
6343
|
-
</annotation>
|
|
6344
|
-
<annotation id="G392">
|
|
6345
|
-
<infon key="type">Gene</infon>
|
|
6346
|
-
<location offset="46385" length="6"/>
|
|
6347
|
-
<text>ndc1-1</text>
|
|
6348
|
-
</annotation>
|
|
6349
|
-
<annotation id="TEAM_277_125">
|
|
6350
|
-
<infon key="type">Gene</infon>
|
|
6351
|
-
<location offset="45121" length="6"/>
|
|
6352
|
-
<text>POM152</text>
|
|
6353
|
-
</annotation>
|
|
6354
|
-
<annotation id="TEAM_277_126">
|
|
6355
|
-
<infon key="type">Gene</infon>
|
|
6356
|
-
<infon key="GeneID">12205</infon>
|
|
6357
|
-
<location offset="45421" length="6"/>
|
|
6358
|
-
<text>pRS315</text>
|
|
6359
|
-
</annotation>
|
|
6360
|
-
<annotation id="TEAM_277_127">
|
|
6361
|
-
<infon key="type">Gene</infon>
|
|
6362
|
-
<location offset="45781" length="6"/>
|
|
6363
|
-
<text>POM152</text>
|
|
6364
|
-
</annotation>
|
|
6365
|
-
<annotation id="TEAM_277_128">
|
|
6366
|
-
<infon key="type">Gene</infon>
|
|
6367
|
-
<infon key="GeneID">200184</infon>
|
|
6368
|
-
<location offset="45789" length="12"/>
|
|
6369
|
-
<text>pLEU2-POM152</text>
|
|
6370
|
-
</annotation>
|
|
6371
3320
|
</passage>
|
|
6372
3321
|
<passage>
|
|
6373
3322
|
<infon key="type">title</infon>
|
|
@@ -6434,13 +3383,6 @@
|
|
|
6434
3383
|
<infon key="type">paragraph</infon>
|
|
6435
3384
|
<offset>46575</offset>
|
|
6436
3385
|
<text>ProA</text>
|
|
6437
|
-
<annotation id="G393">
|
|
6438
|
-
<infon key="score">0.70</infon>
|
|
6439
|
-
<infon key="GeneID">7502638</infon>
|
|
6440
|
-
<infon key="type">Gene</infon>
|
|
6441
|
-
<location offset="46575" length="4"/>
|
|
6442
|
-
<text>ProA</text>
|
|
6443
|
-
</annotation>
|
|
6444
3386
|
</passage>
|
|
6445
3387
|
<passage>
|
|
6446
3388
|
<infon key="type">paragraph</infon>
|
|
@@ -6543,12 +3485,6 @@
|
|
|
6543
3485
|
<location offset="46887" length="7"/>
|
|
6544
3486
|
<text>Pom152p</text>
|
|
6545
3487
|
</annotation>
|
|
6546
|
-
<annotation id="TEAM_277_129">
|
|
6547
|
-
<infon key="type">Gene</infon>
|
|
6548
|
-
<infon key="GeneID">4415</infon>
|
|
6549
|
-
<location offset="46751" length="7"/>
|
|
6550
|
-
<text>Nup157p</text>
|
|
6551
|
-
</annotation>
|
|
6552
3488
|
</passage>
|
|
6553
3489
|
<passage>
|
|
6554
3490
|
<infon key="citation">Ausubel, J.D., R. Brent, R.E. Kingston, D.D. Moore, J.G. Seidman, J.A. Smith, and K. Struhl. 1994. Current Protocols in Molecular Biology. John Wiley and Sons, New York.</infon>
|
|
@@ -6683,12 +3619,6 @@
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|
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|
<infon key="lpage">709</infon>
|
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6684
3620
|
<offset>47346</offset>
|
|
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3621
|
<text>Identification and characterization of a nuclear pore complex protein</text>
|
|
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|
-
<annotation id="TEAM_277_130">
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|
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<infon key="type">Gene</infon>
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|
6688
|
-
<infon key="GeneID">156510</infon>
|
|
6689
|
-
<location offset="47387" length="28"/>
|
|
6690
|
-
<text>nuclear pore complex protein</text>
|
|
6691
|
-
</annotation>
|
|
6692
3622
|
</passage>
|
|
6693
3623
|
<passage>
|
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6694
3624
|
<infon key="source">Proc Natl Acad Sci USA</infon>
|
|
@@ -6853,12 +3783,6 @@
|
|
|
6853
3783
|
<location offset="48396" length="5"/>
|
|
6854
3784
|
<text>NIC96</text>
|
|
6855
3785
|
</annotation>
|
|
6856
|
-
<annotation id="TEAM_277_131">
|
|
6857
|
-
<infon key="type">Gene</infon>
|
|
6858
|
-
<infon key="GeneID">795388</infon>
|
|
6859
|
-
<location offset="48307" length="4"/>
|
|
6860
|
-
<text>NSP1</text>
|
|
6861
|
-
</annotation>
|
|
6862
3786
|
</passage>
|
|
6863
3787
|
<passage>
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6864
3788
|
<infon key="source">EMBO (Eur Mol Biol Organ) J</infon>
|
|
@@ -6898,12 +3822,6 @@
|
|
|
6898
3822
|
<location offset="48519" length="6"/>
|
|
6899
3823
|
<text>Nup57p</text>
|
|
6900
3824
|
</annotation>
|
|
6901
|
-
<annotation id="TEAM_277_132">
|
|
6902
|
-
<infon key="type">Gene</infon>
|
|
6903
|
-
<infon key="GeneID">461059</infon>
|
|
6904
|
-
<location offset="48485" length="5"/>
|
|
6905
|
-
<text>Nsp1p</text>
|
|
6906
|
-
</annotation>
|
|
6907
3825
|
</passage>
|
|
6908
3826
|
<passage>
|
|
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3827
|
<infon key="source">Mol Biol Cell</infon>
|
|
@@ -6940,12 +3858,6 @@
|
|
|
6940
3858
|
<location offset="48565" length="6"/>
|
|
6941
3859
|
<text>Nic96p</text>
|
|
6942
3860
|
</annotation>
|
|
6943
|
-
<annotation id="TEAM_277_133">
|
|
6944
|
-
<infon key="type">Gene</infon>
|
|
6945
|
-
<infon key="GeneID">4415</infon>
|
|
6946
|
-
<location offset="48526" length="5"/>
|
|
6947
|
-
<text>Nup93</text>
|
|
6948
|
-
</annotation>
|
|
6949
3861
|
</passage>
|
|
6950
3862
|
<passage>
|
|
6951
3863
|
<infon key="source">J Cell Biol</infon>
|
|
@@ -6975,12 +3887,6 @@
|
|
|
6975
3887
|
<location offset="48799" length="4"/>
|
|
6976
3888
|
<text>RAT2</text>
|
|
6977
3889
|
</annotation>
|
|
6978
|
-
<annotation id="TEAM_277_134">
|
|
6979
|
-
<infon key="type">Gene</infon>
|
|
6980
|
-
<infon key="GeneID">10114</infon>
|
|
6981
|
-
<location offset="48799" length="4"/>
|
|
6982
|
-
<text>RAT2</text>
|
|
6983
|
-
</annotation>
|
|
6984
3890
|
</passage>
|
|
6985
3891
|
<passage>
|
|
6986
3892
|
<infon key="source">Mol Biol Cell</infon>
|
|
@@ -7022,12 +3928,6 @@
|
|
|
7022
3928
|
<infon key="lpage">2010</infon>
|
|
7023
3929
|
<offset>48912</offset>
|
|
7024
3930
|
<text>Diverse effects of beta-tubulin mutations on microtubule formation and function</text>
|
|
7025
|
-
<annotation id="TEAM_277_135">
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|
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|
-
<infon key="type">Gene</infon>
|
|
7027
|
-
<infon key="GeneID">130530</infon>
|
|
7028
|
-
<location offset="48931" length="12"/>
|
|
7029
|
-
<text>beta-tubulin</text>
|
|
7030
|
-
</annotation>
|
|
7031
3931
|
</passage>
|
|
7032
3932
|
<passage>
|
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7033
3933
|
<infon key="source">J Gen Microbiol</infon>
|
|
@@ -7262,24 +4162,6 @@
|
|
|
7262
4162
|
<location offset="49827" length="6"/>
|
|
7263
4163
|
<text>Nup57p</text>
|
|
7264
4164
|
</annotation>
|
|
7265
|
-
<annotation id="TEAM_277_136">
|
|
7266
|
-
<infon key="type">Gene</infon>
|
|
7267
|
-
<infon key="GeneID">461059</infon>
|
|
7268
|
-
<location offset="49808" length="5"/>
|
|
7269
|
-
<text>Nsp1p</text>
|
|
7270
|
-
</annotation>
|
|
7271
|
-
<annotation id="TEAM_277_137">
|
|
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|
-
<infon key="type">Gene</infon>
|
|
7273
|
-
<infon key="GeneID">4415</infon>
|
|
7274
|
-
<location offset="49815" length="6"/>
|
|
7275
|
-
<text>Nup49p</text>
|
|
7276
|
-
</annotation>
|
|
7277
|
-
<annotation id="TEAM_277_138">
|
|
7278
|
-
<infon key="type">Gene</infon>
|
|
7279
|
-
<infon key="GeneID">4415</infon>
|
|
7280
|
-
<location offset="49827" length="6"/>
|
|
7281
|
-
<text>Nup57p</text>
|
|
7282
|
-
</annotation>
|
|
7283
4165
|
</passage>
|
|
7284
4166
|
<passage>
|
|
7285
4167
|
<infon key="source">Proc Natl Acad Sci USA</infon>
|
|
@@ -7308,12 +4190,6 @@
|
|
|
7308
4190
|
<location offset="49860" length="5"/>
|
|
7309
4191
|
<text>yeast</text>
|
|
7310
4192
|
</annotation>
|
|
7311
|
-
<annotation id="TEAM_277_139">
|
|
7312
|
-
<infon key="type">Gene</infon>
|
|
7313
|
-
<infon key="GeneID">11988</infon>
|
|
7314
|
-
<location offset="49870" length="3"/>
|
|
7315
|
-
<text>TC4</text>
|
|
7316
|
-
</annotation>
|
|
7317
4193
|
</passage>
|
|
7318
4194
|
<passage>
|
|
7319
4195
|
<infon key="source">Mol Biol Cell</infon>
|
|
@@ -7445,11 +4321,6 @@
|
|
|
7445
4321
|
<location offset="50444" length="5"/>
|
|
7446
4322
|
<text>yeast</text>
|
|
7447
4323
|
</annotation>
|
|
7448
|
-
<annotation id="TEAM_277_140">
|
|
7449
|
-
<infon key="type">Gene</infon>
|
|
7450
|
-
<location offset="50450" length="21"/>
|
|
7451
|
-
<text>ribosomal protein L29</text>
|
|
7452
|
-
</annotation>
|
|
7453
4324
|
</passage>
|
|
7454
4325
|
<passage>
|
|
7455
4326
|
<infon key="source">Yeast</infon>
|
|
@@ -7560,11 +4431,6 @@
|
|
|
7560
4431
|
<location offset="50904" length="5"/>
|
|
7561
4432
|
<text>yeast</text>
|
|
7562
4433
|
</annotation>
|
|
7563
|
-
<annotation id="TEAM_277_141">
|
|
7564
|
-
<infon key="type">Gene</infon>
|
|
7565
|
-
<location offset="50855" length="4"/>
|
|
7566
|
-
<text>NDC1</text>
|
|
7567
|
-
</annotation>
|
|
7568
4434
|
</passage>
|
|
7569
4435
|
<passage>
|
|
7570
4436
|
<infon key="source">J Cell Biol</infon>
|
|
@@ -7703,12 +4569,6 @@
|
|
|
7703
4569
|
<location offset="51607" length="7"/>
|
|
7704
4570
|
<text>Nup188p</text>
|
|
7705
4571
|
</annotation>
|
|
7706
|
-
<annotation id="TEAM_277_142">
|
|
7707
|
-
<infon key="type">Gene</infon>
|
|
7708
|
-
<infon key="GeneID">4415</infon>
|
|
7709
|
-
<location offset="51607" length="7"/>
|
|
7710
|
-
<text>Nup188p</text>
|
|
7711
|
-
</annotation>
|
|
7712
4572
|
</passage>
|
|
7713
4573
|
</document>
|
|
7714
4574
|
</collection>
|