htslib 0.0.4 → 0.0.5
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- checksums.yaml +4 -4
- data/lib/hts/bam/record.rb +19 -0
- data/lib/hts/bam.rb +12 -12
- data/lib/hts/bcf/info.rb +58 -5
- data/lib/hts/bcf/record.rb +3 -9
- data/lib/hts/bcf.rb +8 -10
- data/lib/hts/faidx.rb +13 -4
- data/lib/hts/ffi_ext/struct.rb +1 -1
- data/lib/hts/libhts/constants.rb +11 -3
- data/lib/hts/libhts/vcf.rb +27 -0
- data/lib/hts/tabix.rb +13 -5
- data/lib/hts/version.rb +1 -1
- metadata +3 -4
- data/lib/hts/utils/open_method.rb +0 -17
checksums.yaml
CHANGED
@@ -1,7 +1,7 @@
|
|
1
1
|
---
|
2
2
|
SHA256:
|
3
|
-
metadata.gz:
|
4
|
-
data.tar.gz:
|
3
|
+
metadata.gz: 3eb6fbb228a9fa0642c55cdfa39d5189b311df191725954ae46b8dda135dc8c7
|
4
|
+
data.tar.gz: c90ad39aa4919cefa56e534a706bf9659bba9c6ad69eb374491092ce16ae93f6
|
5
5
|
SHA512:
|
6
|
-
metadata.gz:
|
7
|
-
data.tar.gz:
|
6
|
+
metadata.gz: 392ea7c6acb5b86e9e1c1a29f066049c54954edfd277be30541aee433a7349f9d74df09e663ec0269f30d6b87ff47d66a4a668470d3aa536e25a2058cf963546
|
7
|
+
data.tar.gz: 9cc235f8876b8fd4339593eb84a0f2cb8b605b9c82b624cd0f97691b4109a672f55ae90447bba17fb5efcb8257279ad8c2d87234bca390b1624c7aa8f9a7014e
|
data/lib/hts/bam/record.rb
CHANGED
@@ -21,6 +21,8 @@ module HTS
|
|
21
21
|
@bam1.to_ptr
|
22
22
|
end
|
23
23
|
|
24
|
+
attr_reader :header
|
25
|
+
|
24
26
|
# def initialize_copy
|
25
27
|
# super
|
26
28
|
# end
|
@@ -161,6 +163,23 @@ module HTS
|
|
161
163
|
Flag.new(@bam1[:core][:flag])
|
162
164
|
end
|
163
165
|
|
166
|
+
def tag(str)
|
167
|
+
aux = LibHTS.bam_aux_get(@bam1, str)
|
168
|
+
return nil if aux.null?
|
169
|
+
|
170
|
+
t = aux.read_string(1)
|
171
|
+
case t
|
172
|
+
when "i", "I", "c", "C", "s", "S"
|
173
|
+
LibHTS.bam_aux2i(aux)
|
174
|
+
when "f", "d"
|
175
|
+
LibHTS.bam_aux2f(aux)
|
176
|
+
when "Z", "H"
|
177
|
+
LibHTS.bam_aux2Z(aux)
|
178
|
+
when "A"
|
179
|
+
LibHTS.bam_aux2A(aux)
|
180
|
+
end
|
181
|
+
end
|
182
|
+
|
164
183
|
def to_s
|
165
184
|
kstr = LibHTS::KString.new
|
166
185
|
raise "Failed to format bam record" if LibHTS.sam_format1(@header.struct, @bam1, kstr) == -1
|
data/lib/hts/bam.rb
CHANGED
@@ -7,12 +7,10 @@ require_relative "bam/header"
|
|
7
7
|
require_relative "bam/cigar"
|
8
8
|
require_relative "bam/flag"
|
9
9
|
require_relative "bam/record"
|
10
|
-
require_relative "utils/open_method"
|
11
10
|
|
12
11
|
module HTS
|
13
12
|
class Bam
|
14
13
|
include Enumerable
|
15
|
-
extend Utils::OpenMethod
|
16
14
|
|
17
15
|
attr_reader :file_path, :mode, :header
|
18
16
|
# HtfFile is FFI::BitStruct
|
@@ -35,9 +33,6 @@ module HTS
|
|
35
33
|
@htf_file = LibHTS.hts_open(file_path, mode)
|
36
34
|
@header = Bam::Header.new(LibHTS.sam_hdr_read(htf_file))
|
37
35
|
|
38
|
-
# FIXME: should be defined here?
|
39
|
-
@bam1 = LibHTS.bam_init1
|
40
|
-
|
41
36
|
# read
|
42
37
|
if mode[0] == "r"
|
43
38
|
# load index
|
@@ -87,24 +82,29 @@ module HTS
|
|
87
82
|
# LibHTS.bgzf_flush(@htf_file.fp.bgzf)
|
88
83
|
end
|
89
84
|
|
90
|
-
def each
|
85
|
+
def each
|
91
86
|
# Each does not always start at the beginning of the file.
|
92
87
|
# This is the common behavior of IO objects in Ruby.
|
93
88
|
# This may change in the future.
|
94
|
-
|
95
|
-
|
96
|
-
|
89
|
+
return to_enum(__method__) unless block_given?
|
90
|
+
|
91
|
+
while LibHTS.sam_read1(htf_file, header, bam1 = LibHTS.bam_init1) > 0
|
92
|
+
record = Record.new(bam1, header)
|
93
|
+
yield record
|
97
94
|
end
|
95
|
+
self
|
98
96
|
end
|
99
97
|
|
100
98
|
# query [WIP]
|
101
99
|
def query(region)
|
102
100
|
qiter = LibHTS.sam_itr_querys(@idx, header, region)
|
103
101
|
begin
|
104
|
-
|
102
|
+
bam1 = LibHTS.bam_init1
|
103
|
+
slen = LibHTS.sam_itr_next(htf_file, qiter, bam1)
|
105
104
|
while slen > 0
|
106
|
-
yield Record.new(
|
107
|
-
|
105
|
+
yield Record.new(bam1, header)
|
106
|
+
bam1 = LibHTS.bam_init1
|
107
|
+
slen = LibHTS.sam_itr_next(htf_file, qiter, bam1)
|
108
108
|
end
|
109
109
|
ensure
|
110
110
|
LibHTS.hts_itr_destroy(qiter)
|
data/lib/hts/bcf/info.rb
CHANGED
@@ -7,6 +7,7 @@ module HTS
|
|
7
7
|
@record = record
|
8
8
|
end
|
9
9
|
|
10
|
+
# @note Specify the type. If you don't specify a type, it will still work, but it will be slower.
|
10
11
|
def get(key, type = nil)
|
11
12
|
n = FFI::MemoryPointer.new(:int)
|
12
13
|
p1 = @record.p1
|
@@ -20,19 +21,71 @@ module HTS
|
|
20
21
|
p1.read_pointer
|
21
22
|
end
|
22
23
|
|
23
|
-
|
24
|
+
type ||= info_type_to_string(get_info_type(key))
|
25
|
+
|
26
|
+
case type&.to_sym
|
24
27
|
when :int, :int32
|
25
28
|
info_values.call(LibHTS::BCF_HT_INT)
|
26
29
|
.read_array_of_int32(n.read_int)
|
27
30
|
when :float, :real
|
28
31
|
info_values.call(LibHTS::BCF_HT_REAL)
|
29
32
|
.read_array_of_float(n.read_int)
|
30
|
-
when :flag
|
31
|
-
|
32
|
-
|
33
|
+
when :flag, :bool
|
34
|
+
case ret = LibHTS.bcf_get_info_flag(h, r, key, p1, n)
|
35
|
+
when 1 then true
|
36
|
+
when 0 then false
|
37
|
+
when -1 then nil
|
38
|
+
else
|
39
|
+
raise "Unknown return value from bcf_get_info_flag: #{ret}"
|
40
|
+
end
|
33
41
|
when :string, :str
|
34
42
|
info_values.call(LibHTS::BCF_HT_STR)
|
35
|
-
.
|
43
|
+
.read_string
|
44
|
+
end
|
45
|
+
end
|
46
|
+
|
47
|
+
def fields
|
48
|
+
n_info = @record.struct[:n_info]
|
49
|
+
Array.new(n_info) do |i|
|
50
|
+
fld = LibHTS::BcfInfo.new(
|
51
|
+
@record.struct[:d][:info] +
|
52
|
+
i * LibHTS::BcfInfo.size
|
53
|
+
)
|
54
|
+
{
|
55
|
+
name: LibHTS.bcf_hdr_int2id(
|
56
|
+
@record.bcf.header.struct, LibHTS::BCF_DT_ID, fld[:key]
|
57
|
+
),
|
58
|
+
n: LibHTS.bcf_hdr_id2number(
|
59
|
+
@record.bcf.header.struct, LibHTS::BCF_HL_INFO, fld[:key]
|
60
|
+
),
|
61
|
+
vtype: fld[:type], i: fld[:key]
|
62
|
+
}
|
63
|
+
end
|
64
|
+
end
|
65
|
+
|
66
|
+
private
|
67
|
+
|
68
|
+
def get_info_type(key)
|
69
|
+
@record.struct[:n_info].times do |i|
|
70
|
+
fld = LibHTS::BcfInfo.new(
|
71
|
+
@record.struct[:d][:info] +
|
72
|
+
i * LibHTS::BcfInfo.size
|
73
|
+
)
|
74
|
+
id = LibHTS.bcf_hdr_int2id(
|
75
|
+
@record.bcf.header.struct, LibHTS::BCF_DT_ID, fld[:key]
|
76
|
+
)
|
77
|
+
return fld[:type] if id == key
|
78
|
+
end
|
79
|
+
end
|
80
|
+
|
81
|
+
def info_type_to_string(t)
|
82
|
+
case t
|
83
|
+
when 0 then :flag
|
84
|
+
when 1, 2, 3, 4 then :int
|
85
|
+
when 5 then :float
|
86
|
+
when 7 then :string
|
87
|
+
else
|
88
|
+
raise "Unknown info type: #{t}"
|
36
89
|
end
|
37
90
|
end
|
38
91
|
end
|
data/lib/hts/bcf/record.rb
CHANGED
@@ -29,12 +29,7 @@ module HTS
|
|
29
29
|
hdr = @bcf.header.struct
|
30
30
|
rid = @bcf1[:rid]
|
31
31
|
|
32
|
-
|
33
|
-
|
34
|
-
LibHTS::BcfIdpair.new(
|
35
|
-
hdr[:id][LibHTS::BCF_DT_CTG].to_ptr +
|
36
|
-
LibHTS::BcfIdpair.size * rid # offset
|
37
|
-
)[:key]
|
32
|
+
LibHTS.bcf_hdr_id2name(hdr, rid)
|
38
33
|
end
|
39
34
|
|
40
35
|
def pos
|
@@ -106,9 +101,8 @@ module HTS
|
|
106
101
|
|
107
102
|
def to_s
|
108
103
|
ksr = LibHTS::KString.new
|
109
|
-
if LibHTS.vcf_format(@bcf.header.struct, @bcf1, ksr) == -1
|
110
|
-
|
111
|
-
end
|
104
|
+
raise "Failed to format record" if LibHTS.vcf_format(@bcf.header.struct, @bcf1, ksr) == -1
|
105
|
+
|
112
106
|
ksr[:s]
|
113
107
|
end
|
114
108
|
end
|
data/lib/hts/bcf.rb
CHANGED
@@ -4,15 +4,13 @@
|
|
4
4
|
# https://github.com/quinlan-lab/hts-python
|
5
5
|
|
6
6
|
require_relative "bcf/header"
|
7
|
-
require_relative "bcf/record"
|
8
7
|
require_relative "bcf/info"
|
9
8
|
require_relative "bcf/format"
|
10
|
-
require_relative "
|
9
|
+
require_relative "bcf/record"
|
11
10
|
|
12
11
|
module HTS
|
13
12
|
class Bcf
|
14
13
|
include Enumerable
|
15
|
-
extend Utils::OpenMethod
|
16
14
|
|
17
15
|
attr_reader :file_path, :mode, :header
|
18
16
|
# HtfFile is FFI::BitStruct
|
@@ -35,9 +33,6 @@ module HTS
|
|
35
33
|
@htf_file = LibHTS.hts_open(file_path, mode)
|
36
34
|
@header = Bcf::Header.new(LibHTS.bcf_hdr_read(htf_file))
|
37
35
|
|
38
|
-
# FIXME: should be defined here?
|
39
|
-
@bcf1 = LibHTS.bcf_init
|
40
|
-
|
41
36
|
# IO like API
|
42
37
|
if block_given?
|
43
38
|
begin
|
@@ -61,11 +56,14 @@ module HTS
|
|
61
56
|
LibHTS.hts_close(htf_file)
|
62
57
|
end
|
63
58
|
|
64
|
-
def each
|
65
|
-
|
66
|
-
|
67
|
-
|
59
|
+
def each
|
60
|
+
return to_enum(__method__) unless block_given?
|
61
|
+
|
62
|
+
while LibHTS.bcf_read(htf_file, header, bcf1 = LibHTS.bcf_init) != -1
|
63
|
+
record = Record.new(bcf1, self)
|
64
|
+
yield record
|
68
65
|
end
|
66
|
+
self
|
69
67
|
end
|
70
68
|
|
71
69
|
def n_samples
|
data/lib/hts/faidx.rb
CHANGED
@@ -3,17 +3,26 @@
|
|
3
3
|
# Based on hts-python
|
4
4
|
# https://github.com/quinlan-lab/hts-python
|
5
5
|
|
6
|
-
require_relative "utils/open_method"
|
7
|
-
|
8
6
|
module HTS
|
9
7
|
class Faidx
|
10
|
-
extend Utils::OpenMethod
|
11
|
-
|
12
8
|
attr_reader :file_path
|
13
9
|
|
10
|
+
class << self
|
11
|
+
alias open new
|
12
|
+
end
|
13
|
+
|
14
14
|
def initialize(file_path)
|
15
15
|
@file_path = File.expand_path(file_path)
|
16
16
|
@fai = LibHTS.fai_load(file_path)
|
17
|
+
|
18
|
+
# IO like API
|
19
|
+
if block_given?
|
20
|
+
begin
|
21
|
+
yield self
|
22
|
+
ensure
|
23
|
+
close
|
24
|
+
end
|
25
|
+
end
|
17
26
|
end
|
18
27
|
|
19
28
|
def close
|
data/lib/hts/ffi_ext/struct.rb
CHANGED
data/lib/hts/libhts/constants.rb
CHANGED
@@ -289,7 +289,7 @@ module HTS
|
|
289
289
|
:isize, :hts_pos_t
|
290
290
|
end
|
291
291
|
|
292
|
-
class Bam1 < FFI::
|
292
|
+
class Bam1 < FFI::ManagedStruct
|
293
293
|
layout \
|
294
294
|
:core, Bam1Core,
|
295
295
|
:id, :uint64,
|
@@ -301,6 +301,10 @@ module HTS
|
|
301
301
|
# bit_fields :_mempolicy,
|
302
302
|
# :mempolicy, 2,
|
303
303
|
# :_reserved, 30
|
304
|
+
|
305
|
+
def self.release(ptr)
|
306
|
+
LibHTS.bam_destroy1(ptr) unless ptr.null?
|
307
|
+
end
|
304
308
|
end
|
305
309
|
|
306
310
|
typedef :pointer, :bam_plp
|
@@ -457,7 +461,7 @@ module HTS
|
|
457
461
|
:id, :string,
|
458
462
|
:als, :pointer, # (\\0-separated string)
|
459
463
|
:allele, :pointer,
|
460
|
-
:info, BcfInfo.ptr,
|
464
|
+
:info, :pointer, # BcfInfo.ptr,
|
461
465
|
:fmt, BcfFmt.ptr,
|
462
466
|
:var, BcfVariant.ptr,
|
463
467
|
:n_var, :int,
|
@@ -466,7 +470,7 @@ module HTS
|
|
466
470
|
:indiv_dirty, :int
|
467
471
|
end
|
468
472
|
|
469
|
-
class Bcf1 < FFI::
|
473
|
+
class Bcf1 < FFI::ManagedBitStruct
|
470
474
|
layout \
|
471
475
|
:pos, :hts_pos_t,
|
472
476
|
:rlen, :hts_pos_t,
|
@@ -489,6 +493,10 @@ module HTS
|
|
489
493
|
bit_fields :_n_fmt_sample,
|
490
494
|
:n_fmt, 8,
|
491
495
|
:n_sample, 24
|
496
|
+
|
497
|
+
def self.release(ptr)
|
498
|
+
LibHTS.bcf_destroy(ptr) unless ptr.null?
|
499
|
+
end
|
492
500
|
end
|
493
501
|
end
|
494
502
|
end
|
data/lib/hts/libhts/vcf.rb
CHANGED
@@ -167,6 +167,33 @@ module HTS
|
|
167
167
|
LibHTS::BcfIdpair.size * int_id # offsets
|
168
168
|
)[:key]
|
169
169
|
end
|
170
|
+
|
171
|
+
def bcf_hdr_name2id(hdr, id)
|
172
|
+
bcf_hdr_id2int(hdr, BCF_DT_CTG, id)
|
173
|
+
end
|
174
|
+
|
175
|
+
def bcf_hdr_id2name(hdr, rid)
|
176
|
+
return nil if hdr.null? || rid < 0 || rid >= hdr[:n][LibHTS::BCF_DT_CTG]
|
177
|
+
|
178
|
+
LibHTS::BcfIdpair.new(
|
179
|
+
hdr[:id][LibHTS::BCF_DT_CTG].to_ptr +
|
180
|
+
LibHTS::BcfIdpair.size * rid # offset
|
181
|
+
)[:key]
|
182
|
+
end
|
183
|
+
|
184
|
+
def bcf_hdr_id2length(hdr, type, int_id)
|
185
|
+
LibHTS::BcfIdpair.new(
|
186
|
+
hdr[:id][LibHTS::BCF_DT_ID].to_ptr +
|
187
|
+
LibHTS::BcfIdpair.size * int_id # offset
|
188
|
+
)[:val][:info][type] >> 8 & 0xf
|
189
|
+
end
|
190
|
+
|
191
|
+
def bcf_hdr_id2number(hdr, type, int_id)
|
192
|
+
LibHTS::BcfIdpair.new(
|
193
|
+
hdr[:id][LibHTS::BCF_DT_ID].to_ptr +
|
194
|
+
LibHTS::BcfIdpair.size * int_id # offset
|
195
|
+
)[:val][:info][type] >> 12
|
196
|
+
end
|
170
197
|
end
|
171
198
|
|
172
199
|
# constants
|
data/lib/hts/tabix.rb
CHANGED
@@ -3,13 +3,21 @@
|
|
3
3
|
# Based on hts-python
|
4
4
|
# https://github.com/quinlan-lab/hts-python
|
5
5
|
|
6
|
-
require_relative "utils/open_method"
|
7
|
-
|
8
6
|
module HTS
|
9
7
|
class Tabix
|
10
|
-
|
11
|
-
|
12
|
-
|
8
|
+
class << self
|
9
|
+
alias open new
|
10
|
+
end
|
11
|
+
def initialize
|
12
|
+
# IO like API
|
13
|
+
if block_given?
|
14
|
+
begin
|
15
|
+
yield self
|
16
|
+
ensure
|
17
|
+
close
|
18
|
+
end
|
19
|
+
end
|
20
|
+
end
|
13
21
|
|
14
22
|
def build; end
|
15
23
|
|
data/lib/hts/version.rb
CHANGED
metadata
CHANGED
@@ -1,14 +1,14 @@
|
|
1
1
|
--- !ruby/object:Gem::Specification
|
2
2
|
name: htslib
|
3
3
|
version: !ruby/object:Gem::Version
|
4
|
-
version: 0.0.
|
4
|
+
version: 0.0.5
|
5
5
|
platform: ruby
|
6
6
|
authors:
|
7
7
|
- kojix2
|
8
8
|
autorequire:
|
9
9
|
bindir: bin
|
10
10
|
cert_chain: []
|
11
|
-
date: 2021-12-
|
11
|
+
date: 2021-12-26 00:00:00.000000000 Z
|
12
12
|
dependencies:
|
13
13
|
- !ruby/object:Gem::Dependency
|
14
14
|
name: ffi
|
@@ -155,7 +155,6 @@ files:
|
|
155
155
|
- lib/hts/libhts/tbx.rb
|
156
156
|
- lib/hts/libhts/vcf.rb
|
157
157
|
- lib/hts/tabix.rb
|
158
|
-
- lib/hts/utils/open_method.rb
|
159
158
|
- lib/hts/version.rb
|
160
159
|
- lib/htslib.rb
|
161
160
|
homepage: https://github.com/kojix2/ruby-htslib
|
@@ -177,7 +176,7 @@ required_rubygems_version: !ruby/object:Gem::Requirement
|
|
177
176
|
- !ruby/object:Gem::Version
|
178
177
|
version: '0'
|
179
178
|
requirements: []
|
180
|
-
rubygems_version: 3.
|
179
|
+
rubygems_version: 3.3.3
|
181
180
|
signing_key:
|
182
181
|
specification_version: 4
|
183
182
|
summary: HTSlib bindings for Ruby
|