genomer 0.0.5
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- data/.document +5 -0
- data/.gitignore +45 -0
- data/Gemfile +2 -0
- data/LICENSE.txt +20 -0
- data/README.rdoc +23 -0
- data/Rakefile +23 -0
- data/bin/genomer +18 -0
- data/cucumber.yml +2 -0
- data/features/api/annotation_ids.feature +152 -0
- data/features/api/annotation_location.feature +269 -0
- data/features/api/scaffold.feature +38 -0
- data/features/cli/create.feature +19 -0
- data/features/cli/error.feature +17 -0
- data/features/cli/help.feature +48 -0
- data/features/cli/man.feature +71 -0
- data/features/cli/plugins.feature +34 -0
- data/features/step_definitions/genomer_steps.rb +10 -0
- data/features/support/env.rb +13 -0
- data/genomer-plugin-simple/genomer-plugin-simple.gemspec +18 -0
- data/genomer-plugin-simple/lib/genomer-plugin-simple.rb +19 -0
- data/genomer-plugin-simple/man/genomer-simple-subcommand.ronn +6 -0
- data/genomer-plugin-simple/man/genomer-simple.ronn +6 -0
- data/genomer.gemspec +39 -0
- data/lib/genomer.rb +6 -0
- data/lib/genomer/error.rb +4 -0
- data/lib/genomer/plugin.rb +126 -0
- data/lib/genomer/runtime.rb +101 -0
- data/lib/genomer/version.rb +3 -0
- data/man/genomer-init.1.ronn +20 -0
- data/spec/genomer/plugin_spec.rb +341 -0
- data/spec/genomer/runtime_spec.rb +262 -0
- data/spec/spec_helper.rb +45 -0
- metadata +290 -0
data/.document
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data/.gitignore
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tmp
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*.gem
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# rcov generated
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coverage
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# rdoc generated
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rdoc
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# yard generated
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doc
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.yardoc
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# bundler
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.bundle
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# jeweler generated
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pkg
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# Have editor/IDE/OS specific files you need to ignore? Consider using a global gitignore:
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#
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# * Create a file at ~/.gitignore
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# * Include files you want ignored
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# * Run: git config --global core.excludesfile ~/.gitignore
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#
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# After doing this, these files will be ignored in all your git projects,
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# saving you from having to 'pollute' every project you touch with them
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#
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# Not sure what to needs to be ignored for particular editors/OSes? Here's some ideas to get you started. (Remember, remove the leading # of the line)
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#
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# For MacOS:
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#
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#.DS_Store
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#
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# For TextMate
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#*.tmproj
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#tmtags
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#
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# For emacs:
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#*~
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#\#*
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#.\#*
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#
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# For vim:
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#*.swp
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data/Gemfile
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data/LICENSE.txt
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Copyright (c) 2010 Michael Barton
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Permission is hereby granted, free of charge, to any person obtaining
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a copy of this software and associated documentation files (the
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"Software"), to deal in the Software without restriction, including
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without limitation the rights to use, copy, modify, merge, publish,
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distribute, sublicense, and/or sell copies of the Software, and to
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permit persons to whom the Software is furnished to do so, subject to
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the following conditions:
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The above copyright notice and this permission notice shall be
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included in all copies or substantial portions of the Software.
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THE SOFTWARE IS PROVIDED "AS IS", WITHOUT WARRANTY OF ANY KIND,
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EXPRESS OR IMPLIED, INCLUDING BUT NOT LIMITED TO THE WARRANTIES OF
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MERCHANTABILITY, FITNESS FOR A PARTICULAR PURPOSE AND
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NONINFRINGEMENT. IN NO EVENT SHALL THE AUTHORS OR COPYRIGHT HOLDERS BE
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LIABLE FOR ANY CLAIM, DAMAGES OR OTHER LIABILITY, WHETHER IN AN ACTION
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OF CONTRACT, TORT OR OTHERWISE, ARISING FROM, OUT OF OR IN CONNECTION
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WITH THE SOFTWARE OR THE USE OR OTHER DEALINGS IN THE SOFTWARE.
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data/README.rdoc
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== Synopsis
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Genomer is a plugin-based framework for genome finishing. Plugins are installed
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using the RubyGems package management system. Genome files are managed as a
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directory and updated at the command line using the `genomer` command.
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== Installing
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Ruby and RubyGems are required to use genomer and related plugins. Genomer is
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installed on the command line using:
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$ gem install genomer
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== Contact
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Genomer is developed by Michael Barton (www.michaelbarton.me.uk). The source
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code is available on github. Bug reports and feature requests should also be
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made there.
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== Copyright
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Genomer copyright (c) 2010 by Michael Barton. Scaffolder is licensed under the
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MIT license. See LICENSE.txt for further details.
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data/Rakefile
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require 'rubygems'
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require 'bundler'
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begin
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Bundler.setup(:default, :development)
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rescue Bundler::BundlerError => e
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$stderr.puts e.message
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$stderr.puts "Run `bundle install` to install missing gems"
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exit e.status_code
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end
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require 'rake/dsl_definition'
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require 'rspec/core'
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require 'rspec/core/rake_task'
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require 'cucumber/rake/task'
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Bundler::GemHelper.install_tasks
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RSpec::Core::RakeTask.new(:spec) do |spec|
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spec.pattern = FileList['spec/**/*_spec.rb']
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end
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Cucumber::Rake::Task.new(:features)
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task :default => :spec
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data/bin/genomer
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#!/usr/bin/env ruby
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$:.unshift File.join(File.dirname(__FILE__), '..', 'lib')
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require 'rubygems'
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require 'configliere'
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require 'genomer'
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require 'ronn'
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Settings.use :commandline
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Settings.resolve!
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begin
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STDOUT.puts Genomer::Runtime.new(Settings).execute!
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exit true
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rescue Genomer::Error => e
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STDERR.puts "Error. #{e.message}"
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exit false
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end
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data/cucumber.yml
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Feature: Changing annotation IDs
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In order to tailor annotation IDs to specific genomes
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A plugin developer can pass options to the #annotations method
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So that the annotation IDs are correspondingly changed
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@disable-bundler
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Scenario: Adding a prefix to annotation IDs
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Given I run the genomer command with the arguments "init project"
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And I cd to "project"
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And I append to "Gemfile" with:
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"""
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gem 'genomer', :path => '../../../'
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gem 'genomer-plugin-simple', :path => '../../../genomer-plugin-simple'
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"""
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And I append to "assembly/scaffold.yml" with:
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"""
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---
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-
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sequence:
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source: contig1
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"""
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And I append to "assembly/sequence.fna" with:
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"""
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>contig1
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ATGCATGC
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"""
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And I append to "assembly/annotations.gff" with:
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"""
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##gff-version 3
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contig1 . gene 1 4 . + 1 ID=gene1
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contig1 . gene 5 8 . + 1 ID=gene2
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"""
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When I run the genomer command with the arguments "simple annotations --prefix=pre_"
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Then the exit status should be 0
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And the output should contain:
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"""
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##gff-version 3
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scaffold . gene 1 4 . + 1 ID=pre_gene1
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scaffold . gene 5 8 . + 1 ID=pre_gene2
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"""
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@disable-bundler
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Scenario: Reset locus tag numbering from the sequence start
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Given I run the genomer command with the arguments "init project"
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And I cd to "project"
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And I append to "Gemfile" with:
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"""
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gem 'genomer', :path => '../../../'
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gem 'genomer-plugin-simple', :path => '../../../genomer-plugin-simple'
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"""
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And I append to "assembly/scaffold.yml" with:
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"""
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---
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-
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sequence:
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source: contig1
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"""
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And I append to "assembly/sequence.fna" with:
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"""
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>contig1
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ATGCATGC
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"""
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And I append to "assembly/annotations.gff" with:
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"""
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##gff-version 3
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contig1 . gene 1 4 . + 1 ID=gene1
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contig1 . gene 5 8 . + 1 ID=gene2
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"""
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When I run the genomer command with the arguments "simple annotations --reset_locus_numbering"
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Then the exit status should be 0
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And the output should contain:
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"""
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##gff-version 3
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scaffold . gene 1 4 . + 1 ID=000001
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scaffold . gene 5 8 . + 1 ID=000002
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"""
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@disable-bundler
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Scenario: Reset locus tag numbering with at a specific value
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Given I run the genomer command with the arguments "init project"
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And I cd to "project"
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And I append to "Gemfile" with:
|
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"""
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gem 'genomer', :path => '../../../'
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gem 'genomer-plugin-simple', :path => '../../../genomer-plugin-simple'
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"""
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And I append to "assembly/scaffold.yml" with:
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"""
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---
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-
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sequence:
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source: contig1
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"""
|
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And I append to "assembly/sequence.fna" with:
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"""
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>contig1
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ATGCATGC
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"""
|
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And I append to "assembly/annotations.gff" with:
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"""
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##gff-version 3
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contig1 . gene 1 4 . + 1 ID=gene1
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contig1 . gene 5 8 . + 1 ID=gene2
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"""
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When I run the genomer command with the arguments "simple annotations --reset_locus_numbering=10"
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Then the exit status should be 0
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And the output should contain:
|
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"""
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##gff-version 3
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scaffold . gene 1 4 . + 1 ID=000010
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scaffold . gene 5 8 . + 1 ID=000011
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"""
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|
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@disable-bundler
|
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Scenario: Reseting locus tag numbering and adding a prefix
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Given I run the genomer command with the arguments "init project"
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And I cd to "project"
|
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And I append to "Gemfile" with:
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"""
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gem 'genomer', :path => '../../../'
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gem 'genomer-plugin-simple', :path => '../../../genomer-plugin-simple'
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"""
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And I append to "assembly/scaffold.yml" with:
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"""
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---
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-
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sequence:
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source: contig1
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|
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"""
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And I append to "assembly/sequence.fna" with:
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"""
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>contig1
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ATGCATGC
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"""
|
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And I append to "assembly/annotations.gff" with:
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"""
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##gff-version 3
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contig1 . gene 1 4 . + 1 ID=gene1
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contig1 . gene 5 8 . + 1 ID=gene2
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"""
|
145
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When I run the genomer command with the arguments "simple annotations --reset_locus_numbering --prefix=pre_"
|
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Then the exit status should be 0
|
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And the output should contain:
|
148
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"""
|
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##gff-version 3
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scaffold . gene 1 4 . + 1 ID=pre_000001
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scaffold . gene 5 8 . + 1 ID=pre_000002
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"""
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@@ -0,0 +1,269 @@
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1
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Feature: Accessing scaffold annotations
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2
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In order to access gff annotations in a genomer plugin
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A plugin developer can access annotations from the #annotations method
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4
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So that these annotations are available for use
|
5
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+
|
6
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@disable-bundler
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Scenario: Two annotations on a single contig
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Given I run the genomer command with the arguments "init project"
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And I cd to "project"
|
10
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And I append to "Gemfile" with:
|
11
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"""
|
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gem 'genomer', :path => '../../../'
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gem 'genomer-plugin-simple', :path => '../../../genomer-plugin-simple'
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14
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"""
|
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And I append to "assembly/scaffold.yml" with:
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"""
|
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---
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-
|
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sequence:
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source: contig1
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+
|
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"""
|
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And I append to "assembly/sequence.fna" with:
|
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"""
|
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>contig1
|
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ATGCATGC
|
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"""
|
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And I append to "assembly/annotations.gff" with:
|
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"""
|
30
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##gff-version 3
|
31
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contig1 . gene 1 3 . + 1 .
|
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contig1 . gene 5 7 . + 1 .
|
33
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"""
|
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When I run the genomer command with the arguments "simple annotations"
|
35
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+
Then the exit status should be 0
|
36
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+
And the output should contain:
|
37
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+
"""
|
38
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##gff-version 3
|
39
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scaffold . gene 1 3 . + 1 .
|
40
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scaffold . gene 5 7 . + 1 .
|
41
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+
"""
|
42
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+
|
43
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@disable-bundler
|
44
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Scenario: Two annotations on a two contigs
|
45
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+
Given I run the genomer command with the arguments "init project"
|
46
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And I cd to "project"
|
47
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+
And I append to "Gemfile" with:
|
48
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+
"""
|
49
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+
gem 'genomer', :path => '../../../'
|
50
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+
gem 'genomer-plugin-simple', :path => '../../../genomer-plugin-simple'
|
51
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+
"""
|
52
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+
And I append to "assembly/scaffold.yml" with:
|
53
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+
"""
|
54
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---
|
55
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+
-
|
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sequence:
|
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source: contig1
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+
-
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sequence:
|
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source: contig2
|
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+
|
62
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"""
|
63
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And I append to "assembly/sequence.fna" with:
|
64
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"""
|
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>contig1
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ATGCATGC
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>contig2
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ATGCATGC
|
69
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+
"""
|
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And I append to "assembly/annotations.gff" with:
|
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+
"""
|
72
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##gff-version 3
|
73
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contig1 . gene 1 3 . + 1 .
|
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+
contig2 . gene 5 7 . + 1 .
|
75
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+
"""
|
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+
When I run the genomer command with the arguments "simple annotations"
|
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Then the exit status should be 0
|
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And the output should contain:
|
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"""
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##gff-version 3
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|
+
scaffold . gene 1 3 . + 1 .
|
82
|
+
scaffold . gene 13 15 . + 1 .
|
83
|
+
"""
|
84
|
+
|
85
|
+
@disable-bundler
|
86
|
+
Scenario: Two annotations on a single contig with an unused annotation
|
87
|
+
Given I run the genomer command with the arguments "init project"
|
88
|
+
And I cd to "project"
|
89
|
+
And I append to "Gemfile" with:
|
90
|
+
"""
|
91
|
+
gem 'genomer', :path => '../../../'
|
92
|
+
gem 'genomer-plugin-simple', :path => '../../../genomer-plugin-simple'
|
93
|
+
"""
|
94
|
+
And I append to "assembly/scaffold.yml" with:
|
95
|
+
"""
|
96
|
+
---
|
97
|
+
-
|
98
|
+
sequence:
|
99
|
+
source: contig1
|
100
|
+
|
101
|
+
"""
|
102
|
+
And I append to "assembly/sequence.fna" with:
|
103
|
+
"""
|
104
|
+
>contig1
|
105
|
+
ATGCATGC
|
106
|
+
"""
|
107
|
+
And I append to "assembly/annotations.gff" with:
|
108
|
+
"""
|
109
|
+
##gff-version 3
|
110
|
+
contig2 . gene 5 7 . + 1 .
|
111
|
+
contig1 . gene 1 3 . + 1 .
|
112
|
+
contig1 . gene 5 7 . + 1 .
|
113
|
+
"""
|
114
|
+
When I run the genomer command with the arguments "simple annotations"
|
115
|
+
Then the exit status should be 0
|
116
|
+
And the output should contain:
|
117
|
+
"""
|
118
|
+
##gff-version 3
|
119
|
+
scaffold . gene 1 3 . + 1 .
|
120
|
+
scaffold . gene 5 7 . + 1 .
|
121
|
+
"""
|
122
|
+
|
123
|
+
@disable-bundler
|
124
|
+
Scenario: Three unordered annotations on a single contig
|
125
|
+
Given I run the genomer command with the arguments "init project"
|
126
|
+
And I cd to "project"
|
127
|
+
And I append to "Gemfile" with:
|
128
|
+
"""
|
129
|
+
gem 'genomer', :path => '../../../'
|
130
|
+
gem 'genomer-plugin-simple', :path => '../../../genomer-plugin-simple'
|
131
|
+
"""
|
132
|
+
And I append to "assembly/scaffold.yml" with:
|
133
|
+
"""
|
134
|
+
---
|
135
|
+
-
|
136
|
+
sequence:
|
137
|
+
source: contig1
|
138
|
+
|
139
|
+
"""
|
140
|
+
And I append to "assembly/sequence.fna" with:
|
141
|
+
"""
|
142
|
+
>contig1
|
143
|
+
ATGCATGCATGC
|
144
|
+
"""
|
145
|
+
And I append to "assembly/annotations.gff" with:
|
146
|
+
"""
|
147
|
+
##gff-version 3
|
148
|
+
contig1 . gene 9 11 . + 1 .
|
149
|
+
contig1 . gene 1 3 . + 1 .
|
150
|
+
contig1 . gene 5 7 . + 1 .
|
151
|
+
"""
|
152
|
+
When I run the genomer command with the arguments "simple annotations"
|
153
|
+
Then the exit status should be 0
|
154
|
+
And the output should contain:
|
155
|
+
"""
|
156
|
+
##gff-version 3
|
157
|
+
scaffold . gene 1 3 . + 1 .
|
158
|
+
scaffold . gene 5 7 . + 1 .
|
159
|
+
scaffold . gene 9 11 . + 1 .
|
160
|
+
"""
|
161
|
+
|
162
|
+
@disable-bundler
|
163
|
+
Scenario: Four unordered annotations on a two contigs
|
164
|
+
Given I run the genomer command with the arguments "init project"
|
165
|
+
And I cd to "project"
|
166
|
+
And I append to "Gemfile" with:
|
167
|
+
"""
|
168
|
+
gem 'genomer', :path => '../../../'
|
169
|
+
gem 'genomer-plugin-simple', :path => '../../../genomer-plugin-simple'
|
170
|
+
"""
|
171
|
+
And I append to "assembly/scaffold.yml" with:
|
172
|
+
"""
|
173
|
+
---
|
174
|
+
-
|
175
|
+
sequence:
|
176
|
+
source: contig1
|
177
|
+
-
|
178
|
+
sequence:
|
179
|
+
source: contig2
|
180
|
+
|
181
|
+
"""
|
182
|
+
And I append to "assembly/sequence.fna" with:
|
183
|
+
"""
|
184
|
+
>contig1
|
185
|
+
ATGCATGC
|
186
|
+
>contig2
|
187
|
+
ATGCATGC
|
188
|
+
"""
|
189
|
+
And I append to "assembly/annotations.gff" with:
|
190
|
+
"""
|
191
|
+
##gff-version 3
|
192
|
+
contig2 . gene 5 7 . + 1 .
|
193
|
+
contig2 . gene 1 3 . + 1 .
|
194
|
+
contig1 . gene 1 3 . + 1 .
|
195
|
+
contig1 . gene 5 7 . + 1 .
|
196
|
+
"""
|
197
|
+
When I run the genomer command with the arguments "simple annotations"
|
198
|
+
Then the exit status should be 0
|
199
|
+
And the output should contain:
|
200
|
+
"""
|
201
|
+
##gff-version 3
|
202
|
+
scaffold . gene 1 3 . + 1 .
|
203
|
+
scaffold . gene 5 7 . + 1 .
|
204
|
+
scaffold . gene 9 11 . + 1 .
|
205
|
+
scaffold . gene 13 15 . + 1 .
|
206
|
+
|
207
|
+
"""
|
208
|
+
|
209
|
+
@disable-bundler
|
210
|
+
Scenario: Annotations on reversed and trimmed contigs with inserts
|
211
|
+
Given I run the genomer command with the arguments "init project"
|
212
|
+
And I cd to "project"
|
213
|
+
And I append to "Gemfile" with:
|
214
|
+
"""
|
215
|
+
gem 'genomer', :path => '../../../'
|
216
|
+
gem 'genomer-plugin-simple', :path => '../../../genomer-plugin-simple'
|
217
|
+
"""
|
218
|
+
And I append to "assembly/scaffold.yml" with:
|
219
|
+
"""
|
220
|
+
---
|
221
|
+
- sequence:
|
222
|
+
source: contig1
|
223
|
+
stop: 6
|
224
|
+
- sequence:
|
225
|
+
source: contig2
|
226
|
+
reverse: true
|
227
|
+
inserts:
|
228
|
+
-
|
229
|
+
source: insert1
|
230
|
+
open: 6
|
231
|
+
close: 7
|
232
|
+
- sequence:
|
233
|
+
source: contig3
|
234
|
+
start: 3
|
235
|
+
|
236
|
+
"""
|
237
|
+
And I append to "assembly/sequence.fna" with:
|
238
|
+
"""
|
239
|
+
> contig1
|
240
|
+
AAAAAGGG
|
241
|
+
> contig2
|
242
|
+
AAAAAGGGGGC
|
243
|
+
> contig3
|
244
|
+
AAAAAGGG
|
245
|
+
> insert1
|
246
|
+
TTT
|
247
|
+
"""
|
248
|
+
And I append to "assembly/annotations.gff" with:
|
249
|
+
"""
|
250
|
+
##gff-version 3
|
251
|
+
contig1 . gene 1 4 . + 1 ID=gene1
|
252
|
+
contig1 . gene 5 8 . + 1 ID=gene2
|
253
|
+
contig2 . gene 1 4 . + 1 ID=gene3
|
254
|
+
contig2 . gene 8 11 . + 1 ID=gene4
|
255
|
+
contig3 . gene 1 3 . + 1 ID=gene5
|
256
|
+
contig3 . gene 4 8 . + 1 ID=gene6
|
257
|
+
|
258
|
+
"""
|
259
|
+
When I run the genomer command with the arguments "simple annotations"
|
260
|
+
Then the exit status should be 0
|
261
|
+
And the output should contain:
|
262
|
+
"""
|
263
|
+
##gff-version 3
|
264
|
+
scaffold . gene 1 4 . + 1 ID=gene1
|
265
|
+
scaffold . gene 7 10 . - 1 ID=gene4
|
266
|
+
scaffold . gene 15 18 . - 1 ID=gene3
|
267
|
+
scaffold . gene 20 24 . + 1 ID=gene6
|
268
|
+
|
269
|
+
"""
|