flex-station-data 0.1.0

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data/.gitignore

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+/.bundle/
+/.yardoc
+/_yardoc/
+/coverage/
+/doc/
+/pkg/
+/spec/reports/
+/tmp/
+
+# rspec failure tracking
+.rspec_status

data/.rspec

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+--format documentation
+--color
+--require spec_helper

data/CODE_OF_CONDUCT.md

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+# Contributor Covenant Code of Conduct
+
+## Our Pledge
+
+In the interest of fostering an open and welcoming environment, we as
+contributors and maintainers pledge to making participation in our project and
+our community a harassment-free experience for everyone, regardless of age, body
+size, disability, ethnicity, gender identity and expression, level of experience,
+nationality, personal appearance, race, religion, or sexual identity and
+orientation.
+
+## Our Standards
+
+Examples of behavior that contributes to creating a positive environment
+include:
+
+* Using welcoming and inclusive language
+* Being respectful of differing viewpoints and experiences
+* Gracefully accepting constructive criticism
+* Focusing on what is best for the community
+* Showing empathy towards other community members
+
+Examples of unacceptable behavior by participants include:
+
+* The use of sexualized language or imagery and unwelcome sexual attention or
+advances
+* Trolling, insulting/derogatory comments, and personal or political attacks
+* Public or private harassment
+* Publishing others' private information, such as a physical or electronic
+  address, without explicit permission
+* Other conduct which could reasonably be considered inappropriate in a
+  professional setting
+
+## Our Responsibilities
+
+Project maintainers are responsible for clarifying the standards of acceptable
+behavior and are expected to take appropriate and fair corrective action in
+response to any instances of unacceptable behavior.
+
+Project maintainers have the right and responsibility to remove, edit, or
+reject comments, commits, code, wiki edits, issues, and other contributions
+that are not aligned to this Code of Conduct, or to ban temporarily or
+permanently any contributor for other behaviors that they deem inappropriate,
+threatening, offensive, or harmful.
+
+## Scope
+
+This Code of Conduct applies both within project spaces and in public spaces
+when an individual is representing the project or its community. Examples of
+representing a project or community include using an official project e-mail
+address, posting via an official social media account, or acting as an appointed
+representative at an online or offline event. Representation of a project may be
+further defined and clarified by project maintainers.
+
+## Enforcement
+
+Instances of abusive, harassing, or otherwise unacceptable behavior may be
+reported by contacting the project team at john@carney.id.au. All
+complaints will be reviewed and investigated and will result in a response that
+is deemed necessary and appropriate to the circumstances. The project team is
+obligated to maintain confidentiality with regard to the reporter of an incident.
+Further details of specific enforcement policies may be posted separately.
+
+Project maintainers who do not follow or enforce the Code of Conduct in good
+faith may face temporary or permanent repercussions as determined by other
+members of the project's leadership.
+
+## Attribution
+
+This Code of Conduct is adapted from the [Contributor Covenant][homepage], version 1.4,
+available at [http://contributor-covenant.org/version/1/4][version]
+
+[homepage]: http://contributor-covenant.org
+[version]: http://contributor-covenant.org/version/1/4/

data/Gemfile

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+source "https://rubygems.org"
+
+# Specify your gem's dependencies in flex-station-data.gemspec
+gemspec

data/Gemfile.lock

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+PATH
+  remote: .
+  specs:
+    flex-station-data (0.1.0)
+      activesupport
+      linefit
+
+GEM
+  remote: https://rubygems.org/
+  specs:
+    activesupport (6.0.0)
+      concurrent-ruby (~> 1.0, >= 1.0.2)
+      i18n (>= 0.7, < 2)
+      minitest (~> 5.1)
+      tzinfo (~> 1.1)
+      zeitwerk (~> 2.1, >= 2.1.8)
+    coderay (1.1.2)
+    concurrent-ruby (1.1.5)
+    diff-lcs (1.3)
+    i18n (1.6.0)
+      concurrent-ruby (~> 1.0)
+    linefit (0.3.4)
+    method_source (0.9.2)
+    minitest (5.11.3)
+    pry (0.12.2)
+      coderay (~> 1.1.0)
+      method_source (~> 0.9.0)
+    rake (10.5.0)
+    rspec (3.8.0)
+      rspec-core (~> 3.8.0)
+      rspec-expectations (~> 3.8.0)
+      rspec-mocks (~> 3.8.0)
+    rspec-core (3.8.2)
+      rspec-support (~> 3.8.0)
+    rspec-expectations (3.8.4)
+      diff-lcs (>= 1.2.0, < 2.0)
+      rspec-support (~> 3.8.0)
+    rspec-mocks (3.8.1)
+      diff-lcs (>= 1.2.0, < 2.0)
+      rspec-support (~> 3.8.0)
+    rspec-support (3.8.2)
+    thread_safe (0.3.6)
+    tzinfo (1.2.5)
+      thread_safe (~> 0.1)
+    zeitwerk (2.1.9)
+
+PLATFORMS
+  ruby
+
+DEPENDENCIES
+  bundler (~> 2.0)
+  flex-station-data!
+  pry
+  rake (~> 10.0)
+  rspec (~> 3.8)
+
+BUNDLED WITH
+   2.0.2

data/LICENSE.txt

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+The MIT License (MIT)
+
+Copyright (c) 2019 John Carney
+
+Permission is hereby granted, free of charge, to any person obtaining a copy
+of this software and associated documentation files (the "Software"), to deal
+in the Software without restriction, including without limitation the rights
+to use, copy, modify, merge, publish, distribute, sublicense, and/or sell
+copies of the Software, and to permit persons to whom the Software is
+furnished to do so, subject to the following conditions:
+
+The above copyright notice and this permission notice shall be included in
+all copies or substantial portions of the Software.
+
+THE SOFTWARE IS PROVIDED "AS IS", WITHOUT WARRANTY OF ANY KIND, EXPRESS OR
+IMPLIED, INCLUDING BUT NOT LIMITED TO THE WARRANTIES OF MERCHANTABILITY,
+FITNESS FOR A PARTICULAR PURPOSE AND NONINFRINGEMENT. IN NO EVENT SHALL THE
+AUTHORS OR COPYRIGHT HOLDERS BE LIABLE FOR ANY CLAIM, DAMAGES OR OTHER
+LIABILITY, WHETHER IN AN ACTION OF CONTRACT, TORT OR OTHERWISE, ARISING FROM,
+OUT OF OR IN CONNECTION WITH THE SOFTWARE OR THE USE OR OTHER DEALINGS IN
+THE SOFTWARE.

data/README.md

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+# FlexStationData
+
+Welcome to your new gem! In this directory, you'll find the files you need to be able to package up your Ruby library into a gem. Put your Ruby code in the file `lib/flex_station_data`. To experiment with that code, run `bin/console` for an interactive prompt.
+
+TODO: Delete this and the text above, and describe your gem
+
+## Installation
+
+Add this line to your application's Gemfile:
+
+```ruby
+gem 'flex-station-data'
+```
+
+And then execute:
+
+    $ bundle
+
+Or install it yourself as:
+
+    $ gem install flex-station-data
+
+## Usage
+
+TODO: Write usage instructions here
+
+## Development
+
+After checking out the repo, run `bin/setup` to install dependencies. Then, run `rake spec` to run the tests. You can also run `bin/console` for an interactive prompt that will allow you to experiment.
+
+To install this gem onto your local machine, run `bundle exec rake install`. To release a new version, update the version number in `version.rb`, and then run `bundle exec rake release`, which will create a git tag for the version, push git commits and tags, and push the `.gem` file to [rubygems.org](https://rubygems.org).
+
+## Contributing
+
+Bug reports and pull requests are welcome on GitHub at https://github.com/johncarney/flex-station-data. This project is intended to be a safe, welcoming space for collaboration, and contributors are expected to adhere to the [Contributor Covenant](http://contributor-covenant.org) code of conduct.
+
+## License
+
+The gem is available as open source under the terms of the [MIT License](https://opensource.org/licenses/MIT).
+
+## Code of Conduct
+
+Everyone interacting in the FlexStationData project’s codebases, issue trackers, chat rooms and mailing lists is expected to follow the [code of conduct](https://github.com/johncarney/flex-station-data/blob/master/CODE_OF_CONDUCT.md).

data/Rakefile

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+require "bundler/gem_tasks"
+require "rspec/core/rake_task"
+
+RSpec::Core::RakeTask.new(:spec)
+
+task :default => :spec

data/bin/flex-station

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+#!/usr/bin/env ruby
+
+require "pathname"
+
+case command = ARGV.first
+when "sample-data"
+  system(Pathname(__dir__).join("flex-station-sample-data").to_path, *ARGV.drop(1))
+when "linear-regression"
+  system(Pathname(__dir__).join("flex-station-linear-regression").to_path, *ARGV.drop(1))
+when nil
+  $stderr.puts "USAGE: flex-station <command>"
+  exit(1)
+else
+  $stderr.puts "Unrecognised command: #{command}"
+  exit(1)
+end

data/bin/flex-station-linear-regression

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+#!/usr/bin/env ruby
+
+#!/usr/bin/env ruby
+
+require "flex_station_data/services/load_plates"
+require "flex_station_data/linear_regression"
+
+module FlexStationData
+  class LinearRegressionApp
+    include Concerns::Service
+
+    attr_reader :args
+
+    def initialize(*args)
+      @args = args
+    end
+
+    def files
+      @files ||= args.map { |arg| Pathname(arg) }
+    end
+
+    def plates
+      @plates ||= files.map do |file|
+        [ file, LoadPlates.call(file) ]
+      end
+    end
+
+    def call
+      plates.each do |file, file_plates|
+        puts "File: #{file.to_path}"
+        file_plates.each_with_index.map do |plate, plate_index|
+          puts "Plate: #{plate_index + 1}"
+          plate.samples.each do |sample|
+            readings_labels = sample.readings.map(&:label)
+            puts "Sample #{sample.label}"
+            CSV do |out|
+              readings = [*sample.readings, sample.mean]
+              out << ["time", *readings.map(&:label)]
+              plate.times.zip(*readings.map(&:values)).each do |row|
+                out << row
+              end
+
+              regression_factory = LinearRegression.method(:new).curry(2)[plate.times]
+              regressions = readings.map(&:values).map(&regression_factory)
+              out << ["slope", *regressions.map(&:slope)]
+              out << ["R²", *regressions.map(&:r_squared)]
+
+              out << []
+            end
+          end
+        end
+      end
+    end
+  end
+end
+
+FlexStationData::LinearRegressionApp.call(*ARGV)

data/bin/flex-station-sample-data

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+#!/usr/bin/env ruby
+
+require "flex_station_data/services/load_plates"
+
+module FlexStationData
+  class SampleDataApp
+    include Concerns::Service
+
+    attr_reader :args
+
+    def initialize(*args)
+      @args = args
+    end
+
+    def files
+      @files ||= args.map { |arg| Pathname(arg) }
+    end
+
+    def plates
+      @plates ||= files.map do |file|
+        [ file, LoadPlates.call(file) ]
+      end
+    end
+
+    def call
+      plates.each do |file, file_plates|
+        puts "File: #{file.to_path}"
+        file_plates.each_with_index.map do |plate, plate_index|
+          puts "Plate: #{plate_index + 1}"
+          plate.samples.each do |sample|
+            readings_labels = sample.readings.map(&:label)
+            puts "Sample #{sample.label}"
+            CSV do |out|
+              mean = sample.mean
+              out << ["time", *sample.readings.map(&:label), mean.label]
+              plate.times.zip(*sample.readings.map(&:values), mean.values).each do |row|
+                out << row
+              end
+              out << []
+            end
+          end
+        end
+      end
+    end
+  end
+end
+
+FlexStationData::SampleDataApp.call(*ARGV)

data/flex-station-data.gemspec

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+lib = File.expand_path("lib", __dir__)
+$LOAD_PATH.unshift(lib) unless $LOAD_PATH.include?(lib)
+require "flex_station_data/version"
+
+Gem::Specification.new do |spec|
+  spec.name          = "flex-station-data"
+  spec.version       = FlexStationData::VERSION
+  spec.authors       = ["John Carney"]
+  spec.email         = ["john@carney.id.au"]
+
+  spec.summary       = %q{Data analysis tool for FlexStation microplate reader}
+  spec.description   = %q{Data analysis tool for FlexStation microplate reader}
+  spec.homepage      = "https://github.com/johncarney/flex-station-data"
+  spec.license       = "MIT"
+
+  # spec.metadata["allowed_push_host"] = "TODO: Set to 'http://mygemserver.com'"
+
+  spec.metadata["homepage_uri"] = "https://github.com/johncarney/flex-station-data"
+  spec.metadata["source_code_uri"] = "https://github.com/johncarney/flex-station-data"
+  # spec.metadata["changelog_uri"] = "TODO: Put your gem's CHANGELOG.md URL here."
+
+  # Specify which files should be added to the gem when it is released.
+  # The `git ls-files -z` loads the files in the RubyGem that have been added into git.
+  spec.files = Dir.chdir(File.expand_path('..', __FILE__)) do
+    `git ls-files -z`.split("\x0").reject { |f| f.match(%r{^(test|spec|features)/}) }
+  end
+  spec.bindir        = "bin"
+  spec.executables   = spec.files.grep(%r{^bin/}) { |f| File.basename(f) }
+  spec.require_paths = ["lib"]
+
+  spec.required_ruby_version = "~> 2.3"
+
+  spec.add_development_dependency "bundler", "~> 2.0"
+  spec.add_development_dependency "rake", "~> 10.0"
+  spec.add_development_dependency "rspec", "~> 3.8"
+  spec.add_development_dependency "pry"
+
+  spec.add_runtime_dependency "activesupport"
+  spec.add_runtime_dependency "linefit"
+end

data/lib/flex_station_data/concerns/service.rb

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+require "active_support/concern"
+
+module FlexStationData
+  module Concerns
+    module Service
+      extend ActiveSupport::Concern
+
+      class_methods do
+        def call(*args, &block)
+          new(*args).call(&block)
+        end
+
+        def to_proc
+          Proc.new(&method(:call))
+        end
+      end
+    end
+  end
+end

data/lib/flex_station_data/linear_regression.rb

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+require "active_support/core_ext"
+require "linefit"
+
+module FlexStationData
+  class LinearRegression
+    attr_reader :x, :y
+
+    def initialize(x, y)
+      @x = x
+      @y = y
+    end
+
+    def slope
+      coefficients[1]
+    end
+
+    def intercept
+      coefficients[0]
+    end
+
+    def r_squared
+      @r_squared ||= line_fit.rSquared
+    rescue TypeError
+      nil
+    end
+
+    private
+
+    def coefficients
+      @coefficients ||= line_fit.coefficients
+    rescue TypeError
+      [ nil, nil ]
+    end
+
+    def line_fit
+      @line_fit ||= LineFit.new.tap { |lf| lf.setData(x, y) }
+    end
+  end
+end

data/lib/flex_station_data/plate.rb

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+module FlexStationData
+  class Plate
+    attr_reader :times, :temperatures, :samples
+
+    def initialize(times, temperatures, samples)
+      @times = times
+      @temperatures = temperatures
+      @samples = samples
+    end
+  end
+end

data/lib/flex_station_data/readings.rb

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+require "flex_station_data/services/compute_mean"
+
+module FlexStationData
+  class Readings
+    attr_reader :label, :values
+
+    def initialize(label, values)
+      @label = label
+      @values = values
+    end
+
+    def self.mean(*readings, label: "mean")
+      new(label, readings.map(&:values).transpose.map(&ComputeMean))
+    end
+  end
+end

data/lib/flex_station_data/sample.rb

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+require "flex_station_data/readings"
+
+module FlexStationData
+  class Sample
+    attr_reader :label, :readings
+
+    def initialize(label, readings)
+      @label = label
+      @readings = readings
+    end
+
+    def mean
+      @mean ||= Readings.mean(*readings)
+    end
+  end
+end

data/lib/flex_station_data/services/compute_mean.rb

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+require "active_support/core_ext"
+
+require "flex_station_data/concerns/service"
+
+module FlexStationData
+  class ComputeMean
+    include Concerns::Service
+
+    attr_reader :values
+
+    def initialize(values)
+      @values = values
+    end
+
+    def call
+      Float(values.sum) / values.size
+    rescue ArgumentError, TypeError, NoMethodError
+      nil
+    end
+  end
+end

data/lib/flex_station_data/services/load_plates.rb

@@ -0,0 +1,28 @@
+require "csv"
+require "active_support/core_ext"
+
+require "flex_station_data/services/parse_plate"
+
+module FlexStationData
+  class LoadPlates
+    include Concerns::Service
+
+    attr_reader :file
+
+    def initialize(file)
+      @file = file
+    end
+
+    def data
+      CSV.read(file, headers: false).to_a
+    end
+
+    def data_blocks
+      @data_blocks ||= data.split { |row| row[0] == "Plate:" }.drop(1)
+    end
+
+    def call
+      data_blocks.map(&FlexStationData::ParsePlate)
+    end
+  end
+end

data/lib/flex_station_data/services/parse_plate.rb

@@ -0,0 +1,27 @@
+require "active_support/core_ext"
+
+require "flex_station_data/services/parse_plate_readings"
+require "flex_station_data/services/parse_plate_samples"
+require "flex_station_data/plate"
+
+module FlexStationData
+  class ParsePlate
+    include Concerns::Service
+
+    attr_reader :plate_data
+
+    def initialize(plate_data)
+      @plate_data = plate_data
+    end
+
+    def data_blocks
+      plate_data.split { |row| row[0] == "~End" }
+    end
+
+    def call
+      times, temperatures, wells = ParsePlateReadings.call(data_blocks[0])
+      samples = ParsePlateSamples.call(data_blocks[1], wells)
+      Plate.new(times, temperatures, samples)
+    end
+  end
+end

data/lib/flex_station_data/services/parse_plate_readings.rb

@@ -0,0 +1,91 @@
+require "active_support/core_ext"
+
+require "flex_station_data/wells"
+require "flex_station_data/concerns/service"
+
+module FlexStationData
+  class ParsePlateReadings
+    include Concerns::Service
+
+    delegate :parse_time, :parse_value, :parse_row, to: :class
+
+    def initialize(plate_readings_block)
+      @plate_readings_block = plate_readings_block
+    end
+
+    def column_count
+      @column_count ||= headers.reverse.drop_while(&:blank?).size
+    end
+
+    def parsed_rows
+      @parsed_rows ||= begin
+        rows = plate_readings_data.map { |row| parse_row(row[0...column_count]) }
+        rows.select { |row| row.any?(&:present?) }
+      end
+    end
+
+    def parsed_columns
+      @parsed_columns ||= parsed_rows.transpose
+    end
+
+    def times
+      @times ||= parsed_columns[0].compact
+    end
+
+    def temperatures
+      @temperatures ||= parsed_columns[1].compact
+    end
+
+    def values
+      @values ||= parsed_columns.drop(2)
+    end
+
+    def wells_row_count
+      parsed_rows.size / times.size
+    end
+
+    def plate_readings_matrix
+      values.map do |values_column|
+        values_column.each_slice(wells_row_count).to_a.transpose
+      end.transpose
+    end
+
+    def wells
+      Wells.new(plate_readings_matrix)
+    end
+
+    def call
+      [ times, temperatures, wells ]
+    end
+
+    class << self
+      def parse_time(t)
+        return if t.blank?
+
+        h, m, s = t.split(":").map(&:to_i)
+        h * 60.0 + m + s / 60.0
+      end
+
+      def parse_value(v)
+        return Float(v) rescue v.presence
+      end
+
+      def parse_row(row)
+        time, temperature, *values = row
+        [ parse_time(time), parse_value(temperature), *values.map(&method(:parse_value)) ]
+      end
+    end
+
+    private
+
+    def headers
+      plate_readings_block.first
+    end
+
+    def plate_readings_data
+      @plate_readings_data ||= plate_readings_block.drop(1)
+    end
+
+    attr_reader :plate_readings_block
+  end
+end

data/lib/flex_station_data/services/parse_plate_samples.rb

@@ -0,0 +1,49 @@
+require "flex_station_data/readings"
+require "flex_station_data/concerns/service"
+
+require "flex_station_data/sample"
+
+module FlexStationData
+  class ParsePlateSamples
+    include Concerns::Service
+
+    def initialize(sample_map_block, wells)
+      @sample_map_block = sample_map_block
+      @wells = wells
+    end
+
+    def map_rows
+      @map_rows ||= begin
+        rows = sample_map_block.drop_while { |row| row[0] != "Sample" }.drop(1)
+        rows = rows.map { |row| row.map(&:presence) }
+        rows.take_while { |row| row.any?(&:present?) }.to_a
+      end
+    end
+
+    def map_columns
+      @map_columns ||= map_rows.transpose
+    end
+
+    def labels
+      @labels ||= map_columns[0].compact
+    end
+
+    def wells_per_sample
+      map_rows.size / labels.size
+    end
+
+    def sample_map
+      @sample_map ||= map_columns[1].each_slice(wells_per_sample).to_a
+    end
+
+    def call
+      labels.zip(sample_map).map do |label, well_labels|
+        Sample.new(label, well_labels.map(&wells.method(:readings)))
+      end
+    end
+
+    private
+
+    attr_reader :sample_map_block, :wells
+  end
+end

data/lib/flex_station_data/version.rb

@@ -0,0 +1,3 @@
+module FlexStationData
+  VERSION = "0.1.0"
+end

data/lib/flex_station_data/wells.rb

@@ -0,0 +1,29 @@
+require "flex_station_data/readings"
+
+module FlexStationData
+  class Wells
+    def initialize(plate_readings_matrix)
+      @plate_readings_matrix = plate_readings_matrix
+    end
+
+    def readings(well_label)
+      well_index[well_label] ||= begin
+        row, column = parse_well_label(well_label)
+        Readings.new(well_label, plate_readings_matrix[row][column])
+      end
+    end
+
+    def parse_well_label(well_label)
+      row, column = well_label.scan(/\A([A-Z])(\d+)\z/).first
+      [ row.ord - "A".ord, column.to_i - 1 ]
+    end
+
+    private
+
+    def well_index
+      @well_index ||= {}
+    end
+
+    attr_reader :plate_readings_matrix
+  end
+end

data/lib/flex_station_data.rb

@@ -0,0 +1,5 @@
+require "flex_station_data/version"
+
+module FlexStationData
+  class Error < StandardError; end
+end

metadata

@@ -0,0 +1,158 @@
+--- !ruby/object:Gem::Specification
+name: flex-station-data
+version: !ruby/object:Gem::Version
+  version: 0.1.0
+platform: ruby
+authors:
+- John Carney
+autorequire: 
+bindir: bin
+cert_chain: []
+date: 2019-08-22 00:00:00.000000000 Z
+dependencies:
+- !ruby/object:Gem::Dependency
+  name: bundler
+  requirement: !ruby/object:Gem::Requirement
+    requirements:
+    - - "~>"
+      - !ruby/object:Gem::Version
+        version: '2.0'
+  type: :development
+  prerelease: false
+  version_requirements: !ruby/object:Gem::Requirement
+    requirements:
+    - - "~>"
+      - !ruby/object:Gem::Version
+        version: '2.0'
+- !ruby/object:Gem::Dependency
+  name: rake
+  requirement: !ruby/object:Gem::Requirement
+    requirements:
+    - - "~>"
+      - !ruby/object:Gem::Version
+        version: '10.0'
+  type: :development
+  prerelease: false
+  version_requirements: !ruby/object:Gem::Requirement
+    requirements:
+    - - "~>"
+      - !ruby/object:Gem::Version
+        version: '10.0'
+- !ruby/object:Gem::Dependency
+  name: rspec
+  requirement: !ruby/object:Gem::Requirement
+    requirements:
+    - - "~>"
+      - !ruby/object:Gem::Version
+        version: '3.8'
+  type: :development
+  prerelease: false
+  version_requirements: !ruby/object:Gem::Requirement
+    requirements:
+    - - "~>"
+      - !ruby/object:Gem::Version
+        version: '3.8'
+- !ruby/object:Gem::Dependency
+  name: pry
+  requirement: !ruby/object:Gem::Requirement
+    requirements:
+    - - ">="
+      - !ruby/object:Gem::Version
+        version: '0'
+  type: :development
+  prerelease: false
+  version_requirements: !ruby/object:Gem::Requirement
+    requirements:
+    - - ">="
+      - !ruby/object:Gem::Version
+        version: '0'
+- !ruby/object:Gem::Dependency
+  name: activesupport
+  requirement: !ruby/object:Gem::Requirement
+    requirements:
+    - - ">="
+      - !ruby/object:Gem::Version
+        version: '0'
+  type: :runtime
+  prerelease: false
+  version_requirements: !ruby/object:Gem::Requirement
+    requirements:
+    - - ">="
+      - !ruby/object:Gem::Version
+        version: '0'
+- !ruby/object:Gem::Dependency
+  name: linefit
+  requirement: !ruby/object:Gem::Requirement
+    requirements:
+    - - ">="
+      - !ruby/object:Gem::Version
+        version: '0'
+  type: :runtime
+  prerelease: false
+  version_requirements: !ruby/object:Gem::Requirement
+    requirements:
+    - - ">="
+      - !ruby/object:Gem::Version
+        version: '0'
+description: Data analysis tool for FlexStation microplate reader
+email:
+- john@carney.id.au
+executables:
+- flex-station
+- flex-station-linear-regression
+- flex-station-sample-data
+extensions: []
+extra_rdoc_files: []
+files:
+- ".gitignore"
+- ".rspec"
+- CODE_OF_CONDUCT.md
+- Gemfile
+- Gemfile.lock
+- LICENSE.txt
+- README.md
+- Rakefile
+- bin/flex-station
+- bin/flex-station-linear-regression
+- bin/flex-station-sample-data
+- flex-station-data.gemspec
+- lib/flex_station_data.rb
+- lib/flex_station_data/concerns/service.rb
+- lib/flex_station_data/linear_regression.rb
+- lib/flex_station_data/plate.rb
+- lib/flex_station_data/readings.rb
+- lib/flex_station_data/sample.rb
+- lib/flex_station_data/services/compute_mean.rb
+- lib/flex_station_data/services/load_plates.rb
+- lib/flex_station_data/services/parse_plate.rb
+- lib/flex_station_data/services/parse_plate_readings.rb
+- lib/flex_station_data/services/parse_plate_samples.rb
+- lib/flex_station_data/version.rb
+- lib/flex_station_data/wells.rb
+homepage: https://github.com/johncarney/flex-station-data
+licenses:
+- MIT
+metadata:
+  homepage_uri: https://github.com/johncarney/flex-station-data
+  source_code_uri: https://github.com/johncarney/flex-station-data
+post_install_message: 
+rdoc_options: []
+require_paths:
+- lib
+required_ruby_version: !ruby/object:Gem::Requirement
+  requirements:
+  - - "~>"
+    - !ruby/object:Gem::Version
+      version: '2.3'
+required_rubygems_version: !ruby/object:Gem::Requirement
+  requirements:
+  - - ">="
+    - !ruby/object:Gem::Version
+      version: '0'
+requirements: []
+rubyforge_project: 
+rubygems_version: 2.7.6
+signing_key: 
+specification_version: 4
+summary: Data analysis tool for FlexStation microplate reader
+test_files: []