bio-gff3 0.8.7 → 0.9.0
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- data/.travis.yml +12 -0
- data/Gemfile +4 -4
- data/Gemfile.lock +34 -20
- data/README.rdoc +22 -13
- data/Rakefile +24 -23
- data/VERSION +1 -1
- data/bin/gff3-fetch +15 -11
- data/bio-gff3.gemspec +30 -39
- data/lib/bio/db/gff/block/gffblockparser.rb +3 -4
- data/lib/bio/db/gff/digest/gfflrucache.rb +2 -1
- data/lib/bio/db/gff/digest/gffnocache.rb +2 -1
- data/lib/bio/db/gff/digest/gffparser.rb +5 -3
- data/lib/bio/db/gff/gffcomponent.rb +6 -5
- data/lib/bio/db/gff/gffsequence.rb +1 -1
- data/lib/bio/db/gff/gffvalidate.rb +2 -1
- data/spec/gff3_fileiterator_spec.rb +3 -4
- data/test/data/gff/m_hapla.WS232.annotations.part.gff3 +3725 -0
- data/test/data/gff/m_hapla.WS232.genomic.part.fa +3187 -0
- data/test/data/regression/test_ext_gff3.rtest +1 -5
- data/test/data/regression/test_gff3.rtest +1 -5
- data/test/data/regression/test_latest_wormbase_ext_gff3.ref +7885 -0
- data/test/data/regression/test_latest_wormbase_ext_gff3.rtest +280 -0
- data/test/data/regression/test_lrucache_ext_gff3.rtest +1 -3
- data/test/data/regression/test_lrucache_gff3.rtest +1 -3
- data/test/data/regression/test_nocache_ext_gff3.rtest +1 -3
- data/test/data/regression/test_nocache_gff3.rtest +1 -3
- data/test/helper.rb +1 -0
- data/test/{regressiontest.rb → regressiontest2.rb} +5 -5
- data/test/test_bio-gff3.rb +35 -10
- metadata +35 -36
data/.travis.yml
ADDED
@@ -0,0 +1,12 @@
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+
language: ruby
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rvm:
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- 1.8.7
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- 1.9.2
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- 1.9.3
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# - jruby-19mode # JRuby in 1.9 mode
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- rbx-19mode
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# - jruby-18mode # JRuby in 1.8 mode
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# - rbx-18mode
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# uncomment this line if your project needs to run something other than `rake`:
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# script: bundle exec rspec spec
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data/Gemfile
CHANGED
@@ -9,8 +9,8 @@ gem "bio-logger", "> 0.8.0"
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9
9
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# Include everything needed to run rake, tests, features, etc.
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group :development do
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gem "shoulda", ">= 0"
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-
gem "bundler", "~> 1.
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-
gem "jeweler", "~> 1.
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-
gem "
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-
gem "
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+
gem "bundler", "~> 1.1.5"
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gem "jeweler", "~> 1.8.3"
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gem "rspec", ">= 2.10.0"
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gem "regressiontest"
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end
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data/Gemfile.lock
CHANGED
@@ -1,27 +1,41 @@
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1
1
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GEM
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2
2
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remote: http://rubygems.org/
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3
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specs:
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-
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4
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+
activesupport (3.2.7)
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5
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i18n (~> 0.6)
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multi_json (~> 1.0)
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bio (1.4.2)
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bio-logger (1.0.0)
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9
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log4r (>= 1.1.9)
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diff-lcs (1.1.
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diff-lcs (1.1.3)
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git (1.2.5)
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-
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-
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+
i18n (0.6.0)
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jeweler (1.8.4)
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bundler (~> 1.0)
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git (>= 1.2.5)
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rake
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rspec
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rdoc
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json (1.7.4)
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log4r (1.1.10)
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multi_json (1.3.6)
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rake (0.9.2.2)
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rdoc (3.12)
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json (~> 1.4)
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+
regressiontest (0.0.1)
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rspec (2.10.0)
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rspec-core (~> 2.10.0)
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rspec-expectations (~> 2.10.0)
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rspec-mocks (~> 2.10.0)
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rspec-core (2.10.1)
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rspec-expectations (2.10.0)
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diff-lcs (~> 1.1.3)
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+
rspec-mocks (2.10.1)
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shoulda (3.1.1)
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shoulda-context (~> 1.0)
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shoulda-matchers (~> 1.2)
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shoulda-context (1.0.0)
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shoulda-matchers (1.2.0)
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activesupport (>= 3.0.0)
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PLATFORMS
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ruby
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@@ -29,9 +43,9 @@ PLATFORMS
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DEPENDENCIES
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bio (>= 1.3.1)
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bio-logger (> 0.8.0)
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-
bundler (~> 1.
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-
jeweler (~> 1.
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+
bundler (~> 1.1.5)
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jeweler (~> 1.8.3)
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log4r (> 1.1.6)
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-
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rspec (>= 2.
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regressiontest
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rspec (>= 2.10.0)
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shoulda
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data/README.rdoc
CHANGED
@@ -1,3 +1,9 @@
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{<img
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src="https://secure.travis-ci.org/pjotrp/bioruby-gff3-plugin.png"
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/>}[http://travis-ci.org/#!/pjotrp/bioruby-gff3-plugin]
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bio-gff3 is listed at http://biogems.info
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= bio-gff3
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8
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3
9
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GFF3 parser, aimed at parsing big data GFF3 to return sequences of any type,
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@@ -15,7 +21,8 @@ Currently the output is a FASTA file.
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You can use this plugin in two ways. First as a standalone program,
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second as a plugin library to BioRuby.
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-
Note: a really fast GFF3 parser is in the
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Note: a really fast GFF3 parser, and way more flexible, is in the
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works. See https://github.com/mamarjan/gff3-pltools.
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== Install and run gff3-fetch
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@@ -55,13 +62,9 @@ For a write-up see http://thebird.nl/bioruby/BioRuby_GFF3.html
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-------------------------------------------------------------------------------
|
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64
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-
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-
|
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-
Copyright (C) 2010,2011 Pjotr Prins <pjotr.prins@thebird.nl>
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-
|
65
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+
= Command line usage (CLI)
|
62
66
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|
63
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-
|
64
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-
Fetch and assemble GFF3 types (e.g. ORF, mRNA, CDS) + print in FASTA format.
|
67
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+
Fetch and assemble GFF3 types (ORF, mRNA, CDS) + print in FASTA format.
|
65
68
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|
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69
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gff3-fetch [options] type [filename.fa] filename.gff3
|
67
70
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@@ -94,10 +97,10 @@ Copyright (C) 2010,2011 Pjotr Prins <pjotr.prins@thebird.nl>
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94
97
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Where (NYI == Not Yet Implemented):
|
95
98
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|
96
99
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Multiple GFF3 files can be used. With external FASTA files, always the last
|
97
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-
one before the GFF3 filename is matched.
|
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+
one before the GFF3 filename is matched. Make sure the FASTA file
|
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+
comes before the GFF3 file on the command line.
|
98
102
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|
99
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-
Note that above switches are only partially implemented at this stage.
|
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-
feature support is projected Feb. 2011.
|
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+
Note that above switches are only partially implemented at this stage.
|
101
104
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|
102
105
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Examples:
|
103
106
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@@ -164,8 +167,7 @@ time gff3-fetch cds m_hapla.WS217.dna.fa m_hapla.WS217.gff3 2> /dev/null > test.
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|
164
167
|
|
165
168
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Cache real user sys gff3 version
|
166
169
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------------------------------------------------------------
|
167
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-
in preparation
|
168
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-
https://github.com/pjotrp/biolib_hpc/tree/master/modules/gff3
|
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+
in preparation
|
169
171
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------------------------------------------------------------
|
170
172
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|
171
173
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where
|
@@ -176,13 +178,20 @@ where
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|
176
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ruby 1.9.2p136 (2010-12-25 revision 30365) [x86_64-linux]
|
177
179
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on an 8 CPU, 2.6 GHz (6MB cache), 16 GB RAM machine.
|
178
180
|
|
181
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+
Note: the new parser by Marjan is magnitudes faster, and way more
|
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flexible. See https://github.com/mamarjan/gff3-pltools
|
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+
|
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184
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== Cite
|
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185
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|
181
186
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If you use this software, please cite
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187
|
|
188
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http://dx.doi.org/10.1093/bioinformatics/bts080
|
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+
|
190
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+
or
|
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+
|
183
192
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http://dx.doi.org/10.1093/bioinformatics/btq475
|
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== Copyright
|
186
195
|
|
187
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-
Copyright (C) 2010
|
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+
Copyright (C) 2010-2012 Pjotr Prins <pjotr.prins@thebird.nl>
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197
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data/Rakefile
CHANGED
@@ -1,12 +1,12 @@
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require 'rubygems'
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-
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require 'bundler'
|
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begin
|
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Bundler.setup(:default, :development)
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5
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rescue Bundler::BundlerError => e
|
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$stderr.puts e.message
|
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$stderr.puts "Run `bundle install` to install missing gems"
|
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exit e.status_code
|
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end
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require 'rake'
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require 'jeweler'
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@@ -33,7 +33,8 @@ Jeweler::RubygemsDotOrgTasks.new
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33
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require 'rake/testtask'
|
34
34
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Rake::TestTask.new(:test) do |test|
|
35
35
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test.libs << 'lib' << 'test'
|
36
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-
test.pattern = 'test/**/test_*.rb'
|
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# test.pattern = 'test/**/test_*.rb' # breaks in 1.9.3
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test.test_files = Dir.glob("test/**/test_*.rb")
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test.verbose = true
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Kernel.system('rspec spec/*.rb')
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end
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@@ -47,21 +48,21 @@ end
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#end
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require 'rcov/rcovtask'
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Rcov::RcovTask.new do |test|
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-
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-
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-
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end
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# require 'rcov/rcovtask'
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# Rcov::RcovTask.new do |test|
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# test.libs << 'test'
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# test.pattern = 'test/**/test_*.rb'
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# test.verbose = true
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# end
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task :default => :test
|
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require 'rake/rdoctask'
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Rake::RDocTask.new do |rdoc|
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# require 'rake/rdoctask'
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# Rake::RDocTask.new do |rdoc|
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# version = File.exist?('VERSION') ? File.read('VERSION') : ""
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rdoc.rdoc_dir = 'rdoc'
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-
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rdoc.rdoc_files.include('README*')
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rdoc.rdoc_files.include('lib/**/*.rb')
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end
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# rdoc.rdoc_dir = 'rdoc'
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# rdoc.title = "bio-gff3 #{version}"
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# rdoc.rdoc_files.include('README*')
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# rdoc.rdoc_files.include('lib/**/*.rb')
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# end
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data/VERSION
CHANGED
@@ -1 +1 @@
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1
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-
0.
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0.9.0
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data/bin/gff3-fetch
CHANGED
@@ -1,14 +1,13 @@
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1
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#! /usr/bin/ruby
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#! /usr/bin/env ruby
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#
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# Author:: Pjotr Prins
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-
# Copyright:: August 2010
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# License:: Ruby License
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-
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COPYRIGHT = "Copyright (C) 2010-2012 Pjotr Prins <pjotr.prins@thebird.nl>"
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USAGE = <<EOM
|
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-
Fetch and assemble GFF3 types (
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Fetch and assemble GFF3 types (ORF, mRNA, CDS) + print in FASTA format.
|
12
11
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13
12
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gff3-fetch [options] type [filename.fa] filename.gff3
|
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@@ -41,10 +40,10 @@ USAGE = <<EOM
|
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41
40
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Where (NYI == Not Yet Implemented):
|
42
41
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|
43
42
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Multiple GFF3 files can be used. With external FASTA files, always the last
|
44
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-
one before the GFF3 filename is matched.
|
43
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+
one before the GFF3 filename is matched. Make sure the FASTA file
|
44
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+
comes before the GFF3 file on the command line.
|
45
45
|
|
46
|
-
Note that above switches are only partially implemented at this stage.
|
47
|
-
feature support is projected Feb. 2011.
|
46
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+
Note that above switches are only partially implemented at this stage.
|
48
47
|
|
49
48
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Examples:
|
50
49
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@@ -126,11 +125,15 @@ on an 8 CPU, 2.6 GHz (6MB cache), 16 GB RAM machine.
|
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126
125
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|
127
126
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If you use this software, please cite
|
128
127
|
|
128
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+
http://dx.doi.org/10.1093/bioinformatics/bts080
|
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+
|
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or
|
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+
|
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132
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http://dx.doi.org/10.1093/bioinformatics/btq475
|
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133
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== Copyright
|
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135
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-
|
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+
#{COPYRIGHT}
|
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137
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EOM
|
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@@ -144,7 +147,7 @@ require 'optparse'
|
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144
147
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require 'ostruct'
|
145
148
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require 'bio/output/gfffastawriter'
|
146
149
|
|
147
|
-
$stderr.print "
|
150
|
+
$stderr.print "gff3-fetch (biogem Ruby #{RUBY_VERSION}) "+GFF3_VERSION+" "+COPYRIGHT+"\n\n"
|
148
151
|
|
149
152
|
if ARGV.size == 0
|
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print USAGE
|
@@ -158,7 +161,7 @@ options = OpenStruct.new()
|
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# ---- Default options
|
159
162
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options.parser = :line
|
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163
|
|
161
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-
|
164
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+
opts2 = OptionParser.new() { |opts|
|
162
165
|
opts.on_tail("-h", "--help", "Show help and examples") {
|
163
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print(opts)
|
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print USAGE
|
@@ -239,6 +242,7 @@ opts = OptionParser.new() { |opts|
|
|
239
242
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end
|
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243
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241
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}
|
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+
opts = opts2
|
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opts.parse!(ARGV)
|
243
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|
244
248
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Bio::Log::CLI.configure('bio-gff3')
|
data/bio-gff3.gemspec
CHANGED
@@ -4,23 +4,21 @@
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4
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# -*- encoding: utf-8 -*-
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Gem::Specification.new do |s|
|
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s.name =
|
8
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-
s.version = "0.
|
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+
s.name = "bio-gff3"
|
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+
s.version = "0.9.0"
|
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9
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|
10
10
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s.required_rubygems_version = Gem::Requirement.new(">= 0") if s.respond_to? :required_rubygems_version=
|
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-
s.authors = [
|
12
|
-
s.date =
|
13
|
-
s.description =
|
14
|
-
|
15
|
-
|
16
|
-
}
|
17
|
-
s.email = %q{pjotr.prins@thebird.nl}
|
18
|
-
s.executables = [%q{gff3-fetch}]
|
11
|
+
s.authors = ["Pjotr Prins"]
|
12
|
+
s.date = "2012-08-20"
|
13
|
+
s.description = "GFF3 (genome browser) information and digest mRNA and CDS sequences.\nOptions for low memory use and caching of records.\nSupport for external FASTA files.\n"
|
14
|
+
s.email = "pjotr.prins@thebird.nl"
|
15
|
+
s.executables = ["gff3-fetch"]
|
19
16
|
s.extra_rdoc_files = [
|
20
17
|
"LICENSE.txt",
|
21
18
|
"README.rdoc"
|
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]
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s.files = [
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+
".travis.yml",
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"Gemfile",
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"Gemfile.lock",
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"LICENSE.txt",
|
@@ -59,6 +57,8 @@ Support for external FASTA files.
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"test/data/gff/MhA1_Contig1133.gff3",
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"test/data/gff/MhA1_Contig125.fa",
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"test/data/gff/MhA1_Contig125.gff3",
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"test/data/gff/m_hapla.WS232.annotations.part.gff3",
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"test/data/gff/m_hapla.WS232.genomic.part.fa",
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"test/data/gff/standard.gff3",
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"test/data/gff/test-cds.gff3",
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"test/data/gff/test-ext-fasta.fa",
|
@@ -66,30 +66,21 @@ Support for external FASTA files.
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"test/data/gff/test.gff3",
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"test/data/regression/test_ext_gff3.rtest",
|
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"test/data/regression/test_gff3.rtest",
|
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+
"test/data/regression/test_latest_wormbase_ext_gff3.ref",
|
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+
"test/data/regression/test_latest_wormbase_ext_gff3.rtest",
|
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"test/data/regression/test_lrucache_ext_gff3.rtest",
|
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"test/data/regression/test_lrucache_gff3.rtest",
|
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"test/data/regression/test_nocache_ext_gff3.rtest",
|
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"test/data/regression/test_nocache_gff3.rtest",
|
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|
"test/helper.rb",
|
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|
-
"test/
|
75
|
-
"test/test_bio-gff3.rb"
|
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|
-
]
|
77
|
-
s.homepage = %q{https://github.com/pjotrp/bioruby-gff3-plugin}
|
78
|
-
s.licenses = [%q{MIT}]
|
79
|
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s.require_paths = [%q{lib}]
|
80
|
-
s.rubygems_version = %q{1.8.6}
|
81
|
-
s.summary = %q{GFF3 parser for big data}
|
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|
-
s.test_files = [
|
83
|
-
"spec/gff3_assemble2_spec.rb",
|
84
|
-
"spec/gff3_assemble3_spec.rb",
|
85
|
-
"spec/gff3_assemble_spec.rb",
|
86
|
-
"spec/gff3_fileiterator_spec.rb",
|
87
|
-
"spec/gffdb_spec.rb",
|
88
|
-
"spec/gffparserec.rb",
|
89
|
-
"test/helper.rb",
|
90
|
-
"test/regressiontest.rb",
|
76
|
+
"test/regressiontest2.rb",
|
91
77
|
"test/test_bio-gff3.rb"
|
92
78
|
]
|
79
|
+
s.homepage = "https://github.com/pjotrp/bioruby-gff3-plugin"
|
80
|
+
s.licenses = ["MIT"]
|
81
|
+
s.require_paths = ["lib"]
|
82
|
+
s.rubygems_version = "1.8.10"
|
83
|
+
s.summary = "GFF3 parser for big data"
|
93
84
|
|
94
85
|
if s.respond_to? :specification_version then
|
95
86
|
s.specification_version = 3
|
@@ -99,29 +90,29 @@ Support for external FASTA files.
|
|
99
90
|
s.add_runtime_dependency(%q<log4r>, ["> 1.1.6"])
|
100
91
|
s.add_runtime_dependency(%q<bio-logger>, ["> 0.8.0"])
|
101
92
|
s.add_development_dependency(%q<shoulda>, [">= 0"])
|
102
|
-
s.add_development_dependency(%q<bundler>, ["~> 1.
|
103
|
-
s.add_development_dependency(%q<jeweler>, ["~> 1.
|
104
|
-
s.add_development_dependency(%q<
|
105
|
-
s.add_development_dependency(%q<
|
93
|
+
s.add_development_dependency(%q<bundler>, ["~> 1.1.5"])
|
94
|
+
s.add_development_dependency(%q<jeweler>, ["~> 1.8.3"])
|
95
|
+
s.add_development_dependency(%q<rspec>, [">= 2.10.0"])
|
96
|
+
s.add_development_dependency(%q<regressiontest>, [">= 0"])
|
106
97
|
else
|
107
98
|
s.add_dependency(%q<bio>, [">= 1.3.1"])
|
108
99
|
s.add_dependency(%q<log4r>, ["> 1.1.6"])
|
109
100
|
s.add_dependency(%q<bio-logger>, ["> 0.8.0"])
|
110
101
|
s.add_dependency(%q<shoulda>, [">= 0"])
|
111
|
-
s.add_dependency(%q<bundler>, ["~> 1.
|
112
|
-
s.add_dependency(%q<jeweler>, ["~> 1.
|
113
|
-
s.add_dependency(%q<
|
114
|
-
s.add_dependency(%q<
|
102
|
+
s.add_dependency(%q<bundler>, ["~> 1.1.5"])
|
103
|
+
s.add_dependency(%q<jeweler>, ["~> 1.8.3"])
|
104
|
+
s.add_dependency(%q<rspec>, [">= 2.10.0"])
|
105
|
+
s.add_dependency(%q<regressiontest>, [">= 0"])
|
115
106
|
end
|
116
107
|
else
|
117
108
|
s.add_dependency(%q<bio>, [">= 1.3.1"])
|
118
109
|
s.add_dependency(%q<log4r>, ["> 1.1.6"])
|
119
110
|
s.add_dependency(%q<bio-logger>, ["> 0.8.0"])
|
120
111
|
s.add_dependency(%q<shoulda>, [">= 0"])
|
121
|
-
s.add_dependency(%q<bundler>, ["~> 1.
|
122
|
-
s.add_dependency(%q<jeweler>, ["~> 1.
|
123
|
-
s.add_dependency(%q<
|
124
|
-
s.add_dependency(%q<
|
112
|
+
s.add_dependency(%q<bundler>, ["~> 1.1.5"])
|
113
|
+
s.add_dependency(%q<jeweler>, ["~> 1.8.3"])
|
114
|
+
s.add_dependency(%q<rspec>, [">= 2.10.0"])
|
115
|
+
s.add_dependency(%q<regressiontest>, [">= 0"])
|
125
116
|
end
|
126
117
|
end
|
127
118
|
|
@@ -69,23 +69,22 @@ module Bio
|
|
69
69
|
|
70
70
|
def each_seq(gfftype)
|
71
71
|
parse(gfftype) { | id, seq | yield id,seq }
|
72
|
-
|
73
72
|
end
|
74
73
|
|
75
74
|
def each_gene_seq
|
76
75
|
each_seq('gene') { | id, seq | yield id,seq }
|
77
76
|
end
|
77
|
+
|
78
78
|
def each_mRNA_seq
|
79
79
|
each_seq('mrna') { | id, seq | yield id,seq }
|
80
|
-
|
81
80
|
end
|
81
|
+
|
82
82
|
def each_exon_seq
|
83
83
|
each_seq('exon') { | id, seq | yield id,seq }
|
84
|
-
|
85
84
|
end
|
85
|
+
|
86
86
|
def each_CDS_seq
|
87
87
|
each_seq('cds') { | id, seq | yield id,seq }
|
88
|
-
|
89
88
|
end
|
90
89
|
end
|
91
90
|
end
|