bettersam 0.0.3 → 0.1.0
Sign up to get free protection for your applications and to get access to all the features.
- checksums.yaml +4 -4
- data/lib/bettersam.rb +22 -8
- data/test/test_test.rb +10 -2
- metadata +42 -17
checksums.yaml
CHANGED
@@ -1,7 +1,7 @@
|
|
1
1
|
---
|
2
2
|
SHA1:
|
3
|
-
metadata.gz:
|
4
|
-
data.tar.gz:
|
3
|
+
metadata.gz: b784a8ef5c7c567134964e9d028157ae3ef14579
|
4
|
+
data.tar.gz: 7ac15b849c7402b8dcd3de17ef9a5d2c52cfabda
|
5
5
|
SHA512:
|
6
|
-
metadata.gz:
|
7
|
-
data.tar.gz:
|
6
|
+
metadata.gz: 8d492bc114f9a8a195eb47a34302539e26494d809bd4a01e2710358bd75e53ed8688dd58b7514cfb4f4c77835c213534bebf7a125a2084be2db5cd097f12a5c2
|
7
|
+
data.tar.gz: 66eed73b517ad1b0710e7f81664136476801753c1fda5e0e50f123f80ced5a35f6805a5998d0291c53ced983dd610c9b00881ef4261da55a32fc69cebb9b993b
|
data/lib/bettersam.rb
CHANGED
@@ -18,8 +18,9 @@ class BetterSam
|
|
18
18
|
0x400] # 11. read is PCR or optical duplicate
|
19
19
|
|
20
20
|
public
|
21
|
-
attr_accessor :name, :flag, :chrom, :pos, :mapq, :cigar, :mchrom, :mpos
|
22
|
-
attr_accessor :
|
21
|
+
attr_accessor :name, :flag, :chrom, :pos, :mapq, :cigar, :mchrom, :mpos
|
22
|
+
attr_accessor :insert, :seq, :qual, :tags
|
23
|
+
attr_accessor :snp, :length
|
23
24
|
attr_reader :cigar_list
|
24
25
|
|
25
26
|
def initialize(line=nil)
|
@@ -52,11 +53,15 @@ public
|
|
52
53
|
while i < f.size
|
53
54
|
tag = f[i]
|
54
55
|
i += 1
|
55
|
-
|
56
|
-
raise line if
|
57
|
-
|
58
|
-
|
59
|
-
|
56
|
+
a = tag.split(":")
|
57
|
+
raise line if a.length != 3
|
58
|
+
if a[1]=="i"
|
59
|
+
@tags[a[0].to_sym] = a[2].to_i
|
60
|
+
elsif a[1]=="Z"
|
61
|
+
@tags[a[0].to_sym] = a[2]
|
62
|
+
else
|
63
|
+
@tags[a[0].to_sym] = a[2]
|
64
|
+
end
|
60
65
|
end
|
61
66
|
|
62
67
|
return true;
|
@@ -123,10 +128,19 @@ public
|
|
123
128
|
!self.pair_opposite_strands?
|
124
129
|
end
|
125
130
|
|
131
|
+
def edit_distance
|
132
|
+
@tags[:NM]
|
133
|
+
end
|
134
|
+
|
135
|
+
def length
|
136
|
+
@length = @seq.length if !@length
|
137
|
+
return @length
|
138
|
+
end
|
139
|
+
|
126
140
|
# cigar parsing methods
|
127
141
|
|
128
142
|
def exact_match?
|
129
|
-
@
|
143
|
+
@tags[:NM]==0
|
130
144
|
end
|
131
145
|
|
132
146
|
def endpos
|
data/test/test_test.rb
CHANGED
@@ -8,12 +8,12 @@ class TestBetterSam < Test::Unit::TestCase
|
|
8
8
|
|
9
9
|
setup do
|
10
10
|
# this is run before each test
|
11
|
-
@l1 = BetterSam.new("FCC00CKABXX:2:1101:10117:6470#CAGATCAT 81 nivara_3s 1572276 40 100M = 1571527 -849 AGGATCGGGCCTCGTGAGCCGACGGTGAGCGAGTTGTTGTTGTTCCATACGGGGGCGCCGGAGTTGGTGCTCCACAGCGGGCCGTTGAACGAGCTCGACG ZbaX^_baX\_S]_ZdYccYebeffddZdbebdadc[bdVeeeceeeddggggggggggggggggegeggdffbfefegggggggggggggggggggggg AS:i:-24 XN:i:0 XM:i:4 XO:i:0 XG:i:0 NM:i:
|
11
|
+
@l1 = BetterSam.new("FCC00CKABXX:2:1101:10117:6470#CAGATCAT 81 nivara_3s 1572276 40 100M = 1571527 -849 AGGATCGGGCCTCGTGAGCCGACGGTGAGCGAGTTGTTGTTGTTCCATACGGGGGCGCCGGAGTTGGTGCTCCACAGCGGGCCGTTGAACGAGCTCGACG ZbaX^_baX\_S]_ZdYccYebeffddZdbebdadc[bdVeeeceeeddggggggggggggggggegeggdffbfefegggggggggggggggggggggg AS:i:-24 XN:i:0 XM:i:4 XO:i:0 XG:i:0 NM:i:0 MD:Z:1T1G3T0A91 YS:i:-5 YT:Z:DP")
|
12
12
|
@l2 = BetterSam.new("FCC00CKABXX:2:1101:10117:6470#CAGATCAT 81 chromosome03 1789384 24 4M5I91M = 1788782 -697 AGGATCGGGCCTCGTGAGCCGACGGTGAGCGAGTTGTTGTTGTTCCATACGGGGGCGCCGGAGTTGGTGCTCCACAGCGGGCCGTTGAACGAGCTCGACG ZbaX^_baX\_S]_ZdYccYebeffddZdbebdadc[bdVeeeceeeddggggggggggggggggegeggdffbfefegggggggggggggggggggggg AS:i:-38 XN:i:0 XM:i:3 XO:i:1 XG:i:5 NM:i:8 MD:Z:0C1T6G85 YS:i:-5 YT:Z:DP")
|
13
13
|
@l3 = BetterSam.new("FCC00CKABXX:2:1101:19524:66398#CAGATCAT 145 chromosome03 1789377 23 4M1I2M1D93M = 1788766 -711 GGAGGATCGGGCCTCGTGGGCCGACGGTGAGCGAGTTGTTGTTGTTCCATACGGGGGCGCCGGAGTTGGTGCTCCACAGCGGGCCGTTGAACGAGCTCGA Bc`aaT\Y_]RLMKKMHEMV_T[Y[deaeeeaadbaaa\_feecedddddadfcegdcXdggcggggggggg`gfbecbcggggggggeggggggggggg AS:i:-51 XN:i:0 XM:i:6 XO:i:2 XG:i:2 NM:i:8 MD:Z:2T0C2^A2T0A5G1A81 YS:i:0 YT:Z:DP")
|
14
14
|
@l4l = BetterSam.new("FCC00CKABXX:2:1101:16909:83925#CAGATCAT 145 nivara_3s 1572267 23 5M2D3M2I3M1I86M = 1571498 -868 GTCCTCCAGGAGGATCGGGCCTCGTGAGCCGACGGTGAGCGAGTTGTTGTTGTTCCATACGGGGGCGCCGGAGTTGGTGCTCCACAGCGGGCCGTTGAAC BBBBBB_Z`cU]^SZS][]USKV[L`ac`dedeageeefagegagffdd`egedgggedgggggggdggggggggefeeeQgeagggggggggggggggg AS:i:-53 XN:i:0 XM:i:4 XO:i:3 XG:i:5 NM:i:9 MD:Z:2G2^TG3T5T0A81 YS:i:0 YT:Z:DP")
|
15
15
|
@l4r = BetterSam.new("FCC00CKABXX:2:1101:16909:83925#CAGATCAT 145 chromosome03 1789378 23 7M4I3M5I81M = 1788753 -716 GTCCTCCAGGAGGATCGGGCCTCGTGAGCCGACGGTGAGCGAGTTGTTGTTGTTCCATACGGGGGCGCCGGAGTTGGTGCTCCACAGCGGGCCGTTGAAC BBBBBB_Z`cU]^SZS][]USKV[L`ac`dedeageeefagegagffdd`egedgggedgggggggdggggggggefeeeQgeagggggggggggggggg AS:i:-59 XN:i:0 XM:i:4 XO:i:2 XG:i:9 NM:i:13 MD:Z:3A1A2T6G75 YS:i:0 YT:Z:DP")
|
16
|
-
@l5l = BetterSam.new("FCC2HFRACXX:7:2314:9299:67450#TGACCAAT 355 Sb02g000720.1 1186 18 71M = 1238 -150 CGTCATCTTCTCTCATATATTTGTATCACCCATCCATCCATCTGCCTTCGATATGCATCTCCACTCCGCCG __^cc]^\`eegea`ffdfghhfd]eghhfffef``degfhf_^gdfhfg_fghhhfdhffdfhffbeWcW AS:i:142 XN:i:0 XM:i:0 XO:i:0 XG:i:0
|
16
|
+
@l5l = BetterSam.new("FCC2HFRACXX:7:2314:9299:67450#TGACCAAT 355 Sb02g000720.1 1186 18 71M = 1238 -150 CGTCATCTTCTCTCATATATTTGTATCACCCATCCATCCATCTGCCTTCGATATGCATCTCCACTCCGCCG __^cc]^\`eegea`ffdfghhfd]eghhfffef``degfhf_^gdfhfg_fghhhfdhffdfhffbeWcW AS:i:142 XN:i:0 XM:i:0 XO:i:0 XG:i:0 MD:Z:71 YS:i:44 YT:Z:CP") # NM:i:0
|
17
17
|
end
|
18
18
|
|
19
19
|
should "be a paired read" do
|
@@ -94,5 +94,13 @@ class TestBetterSam < Test::Unit::TestCase
|
|
94
94
|
assert !@l5l.primary_aln?
|
95
95
|
end
|
96
96
|
|
97
|
+
should "get the edit distance" do
|
98
|
+
assert_equal 8, @l2.tags[:NM]
|
99
|
+
end
|
100
|
+
|
101
|
+
should "fail to get the edit distance" do
|
102
|
+
assert_equal nil, @l5l.tags[:NM]
|
103
|
+
end
|
104
|
+
|
97
105
|
end
|
98
106
|
end
|
metadata
CHANGED
@@ -1,86 +1,111 @@
|
|
1
1
|
--- !ruby/object:Gem::Specification
|
2
2
|
name: bettersam
|
3
3
|
version: !ruby/object:Gem::Version
|
4
|
-
version: 0.0
|
4
|
+
version: 0.1.0
|
5
5
|
platform: ruby
|
6
6
|
authors:
|
7
|
-
- Richard Smith
|
7
|
+
- Richard Smith-Unna
|
8
8
|
- Chris Boursnell
|
9
|
+
- Jesse Rodriguez
|
9
10
|
autorequire:
|
10
11
|
bindir: bin
|
11
12
|
cert_chain: []
|
12
|
-
date: 2014-
|
13
|
+
date: 2014-08-05 00:00:00.000000000 Z
|
13
14
|
dependencies:
|
14
15
|
- !ruby/object:Gem::Dependency
|
15
16
|
name: simplecov
|
16
17
|
requirement: !ruby/object:Gem::Requirement
|
17
18
|
requirements:
|
19
|
+
- - ~>
|
20
|
+
- !ruby/object:Gem::Version
|
21
|
+
version: '0.8'
|
18
22
|
- - '>='
|
19
23
|
- !ruby/object:Gem::Version
|
20
|
-
version:
|
24
|
+
version: 0.8.2
|
21
25
|
type: :development
|
22
26
|
prerelease: false
|
23
27
|
version_requirements: !ruby/object:Gem::Requirement
|
24
28
|
requirements:
|
29
|
+
- - ~>
|
30
|
+
- !ruby/object:Gem::Version
|
31
|
+
version: '0.8'
|
25
32
|
- - '>='
|
26
33
|
- !ruby/object:Gem::Version
|
27
|
-
version:
|
34
|
+
version: 0.8.2
|
28
35
|
- !ruby/object:Gem::Dependency
|
29
36
|
name: rake
|
30
37
|
requirement: !ruby/object:Gem::Requirement
|
31
38
|
requirements:
|
39
|
+
- - ~>
|
40
|
+
- !ruby/object:Gem::Version
|
41
|
+
version: '10.3'
|
32
42
|
- - '>='
|
33
43
|
- !ruby/object:Gem::Version
|
34
|
-
version:
|
44
|
+
version: 10.3.2
|
35
45
|
type: :development
|
36
46
|
prerelease: false
|
37
47
|
version_requirements: !ruby/object:Gem::Requirement
|
38
48
|
requirements:
|
49
|
+
- - ~>
|
50
|
+
- !ruby/object:Gem::Version
|
51
|
+
version: '10.3'
|
39
52
|
- - '>='
|
40
53
|
- !ruby/object:Gem::Version
|
41
|
-
version:
|
54
|
+
version: 10.3.2
|
42
55
|
- !ruby/object:Gem::Dependency
|
43
56
|
name: turn
|
44
57
|
requirement: !ruby/object:Gem::Requirement
|
45
58
|
requirements:
|
59
|
+
- - ~>
|
60
|
+
- !ruby/object:Gem::Version
|
61
|
+
version: '0.9'
|
46
62
|
- - '>='
|
47
63
|
- !ruby/object:Gem::Version
|
48
|
-
version:
|
64
|
+
version: 0.9.7
|
49
65
|
type: :development
|
50
66
|
prerelease: false
|
51
67
|
version_requirements: !ruby/object:Gem::Requirement
|
52
68
|
requirements:
|
69
|
+
- - ~>
|
70
|
+
- !ruby/object:Gem::Version
|
71
|
+
version: '0.9'
|
53
72
|
- - '>='
|
54
73
|
- !ruby/object:Gem::Version
|
55
|
-
version:
|
74
|
+
version: 0.9.7
|
56
75
|
- !ruby/object:Gem::Dependency
|
57
76
|
name: shoulda-context
|
58
77
|
requirement: !ruby/object:Gem::Requirement
|
59
78
|
requirements:
|
79
|
+
- - ~>
|
80
|
+
- !ruby/object:Gem::Version
|
81
|
+
version: '1.2'
|
60
82
|
- - '>='
|
61
83
|
- !ruby/object:Gem::Version
|
62
|
-
version:
|
84
|
+
version: 1.2.1
|
63
85
|
type: :development
|
64
86
|
prerelease: false
|
65
87
|
version_requirements: !ruby/object:Gem::Requirement
|
66
88
|
requirements:
|
89
|
+
- - ~>
|
90
|
+
- !ruby/object:Gem::Version
|
91
|
+
version: '1.2'
|
67
92
|
- - '>='
|
68
93
|
- !ruby/object:Gem::Version
|
69
|
-
version:
|
94
|
+
version: 1.2.1
|
70
95
|
- !ruby/object:Gem::Dependency
|
71
96
|
name: coveralls
|
72
97
|
requirement: !ruby/object:Gem::Requirement
|
73
98
|
requirements:
|
74
|
-
- -
|
99
|
+
- - ~>
|
75
100
|
- !ruby/object:Gem::Version
|
76
|
-
version: '0'
|
101
|
+
version: '0.7'
|
77
102
|
type: :development
|
78
103
|
prerelease: false
|
79
104
|
version_requirements: !ruby/object:Gem::Requirement
|
80
105
|
requirements:
|
81
|
-
- -
|
106
|
+
- - ~>
|
82
107
|
- !ruby/object:Gem::Version
|
83
|
-
version: '0'
|
108
|
+
version: '0.7'
|
84
109
|
description: Extended SAM (Sequence Alignment/Map) file parsing
|
85
110
|
email: rds45@cam.ac.uk
|
86
111
|
executables: []
|
@@ -89,8 +114,8 @@ extra_rdoc_files: []
|
|
89
114
|
files:
|
90
115
|
- Rakefile
|
91
116
|
- lib/bettersam.rb
|
92
|
-
- test/helper.rb
|
93
117
|
- test/test_test.rb
|
118
|
+
- test/helper.rb
|
94
119
|
- README.md
|
95
120
|
- LICENSE
|
96
121
|
homepage: https://github.com/blahah/bettersam
|
@@ -113,7 +138,7 @@ required_rubygems_version: !ruby/object:Gem::Requirement
|
|
113
138
|
version: '0'
|
114
139
|
requirements: []
|
115
140
|
rubyforge_project:
|
116
|
-
rubygems_version: 2.
|
141
|
+
rubygems_version: 2.1.4
|
117
142
|
signing_key:
|
118
143
|
specification_version: 4
|
119
144
|
summary: Extended SAM file parsing
|